Planarian EST Database


Dr_sW_024_O17

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_024_O17
         (554 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P42648|14331_ENTHI  14-3-3 protein 1 (14-3-3-1)                229   3e-60
sp|P42649|14332_ENTHI  14-3-3 protein 2 (14-3-3-2)                227   1e-59
sp|P92177|1433E_DROME  14-3-3 protein epsilon (Suppressor of...   226   4e-59
sp|P42650|14333_ENTHI  14-3-3 protein 3 (14-3-3-3)                223   2e-58
sp|Q99002|1433_TRIHA  14-3-3 protein homolog (Th1433)             220   2e-57
sp|Q96453|1433D_SOYBN  14-3-3-like protein D (SGF14D)             219   3e-57
sp|P48347|14310_ARATH  14-3-3-like protein GF14 epsilon (Gen...   219   4e-57
sp|P93212|1437_LYCES  14-3-3 protein 7                            219   5e-57
sp|P34730|BMH2_YEAST  Protein BMH2                                218   6e-57
sp|P93208|14332_LYCES  14-3-3 protein 2                           218   6e-57
>sp|P42648|14331_ENTHI 14-3-3 protein 1 (14-3-3-1)
          Length = 239

 Score =  229 bits (585), Expect = 3e-60
 Identities = 117/184 (63%), Positives = 152/184 (82%), Gaps = 2/184 (1%)
 Frame = +3

Query: 9   MADREENVYRAKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASW 188
           MA RE+ VY AKLAEQ+ERYDEMV+ MK VA  + ELS+EERNLLSVA+KNVIG++RASW
Sbjct: 1   MASREDCVYTAKLAEQSERYDEMVQCMKQVAEMEAELSIEERNLLSVAYKNVIGAKRASW 60

Query: 189 RILSTIEQREEAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQV 368
           RI+S++EQ+E+AKG+ +  +E+ K YR ++EKEL+  CD+V+KV+   LLPKAS +ES+V
Sbjct: 61  RIISSLEQKEQAKGNDK-HVEIIKGYRAKIEKELSTCCDDVLKVIQENLLPKASTSESKV 119

Query: 369 FYLKMKGDYYRYRAEFAIEKEREEVAQKSLGAYESA--MTEAESLSATHPIRLGLALNFS 542
           F+ KM+GDYYRY AEF ++++R+EVA KSL AY  A  ++ AE L+ THPIRLGLALNFS
Sbjct: 120 FFKKMEGDYYRYFAEFTVDEKRKEVADKSLAAYTEATEISNAE-LAPTHPIRLGLALNFS 178

Query: 543 VFYF 554
           VFYF
Sbjct: 179 VFYF 182
>sp|P42649|14332_ENTHI 14-3-3 protein 2 (14-3-3-2)
          Length = 238

 Score =  227 bits (579), Expect = 1e-59
 Identities = 112/182 (61%), Positives = 150/182 (82%)
 Frame = +3

Query: 9   MADREENVYRAKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASW 188
           M  RE+ VY +KLAEQ+ERY+EMV+ MK VA   TELSVEERNL+SVA+KNV+GSRRASW
Sbjct: 1   MTSREDLVYLSKLAEQSERYEEMVQYMKQVAEMGTELSVEERNLISVAYKNVVGSRRASW 60

Query: 189 RILSTIEQREEAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQV 368
           RI+S++EQ+E+AKG++Q R+EL K YR ++E+EL++ CD+V+K++   LL  +++ ES+V
Sbjct: 61  RIISSLEQKEQAKGNTQ-RVELIKTYRAKIEQELSQKCDDVLKIITEFLLKNSTSIESKV 119

Query: 369 FYLKMKGDYYRYRAEFAIEKEREEVAQKSLGAYESAMTEAESLSATHPIRLGLALNFSVF 548
           F+ KM+GDYYRY AEF ++++R+EVA KSL AY+ A   A SL  THPIRLGLALNFSVF
Sbjct: 120 FFKKMEGDYYRYYAEFTVDEKRKEVADKSLAAYQEATDTAASLVPTHPIRLGLALNFSVF 179

Query: 549 YF 554
           Y+
Sbjct: 180 YY 181
>sp|P92177|1433E_DROME 14-3-3 protein epsilon (Suppressor of Ras1 3-9)
          Length = 262

 Score =  226 bits (575), Expect = 4e-59
 Identities = 114/183 (62%), Positives = 149/183 (81%), Gaps = 1/183 (0%)
 Frame = +3

Query: 9   MADREENVYRAKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASW 188
           M +RE NVY+AKLAEQAERYDEMV++MK VA+ D EL+VEERNLLSVA+KNVIG+RRASW
Sbjct: 1   MTERENNVYKAKLAEQAERYDEMVEAMKKVASMDVELTVEERNLLSVAYKNVIGARRASW 60

Query: 189 RILSTIEQREEAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQV 368
           RI+++IEQ+EE KG+ + ++E+ K YR QVEKEL ++C +++ VL+  L+P A++ ES+V
Sbjct: 61  RIITSIEQKEENKGAEE-KLEMIKTYRGQVEKELRDICSDILNVLEKHLIPCATSGESKV 119

Query: 369 FYLKMKGDYYRYRAEFAIEKEREEVAQKSLGAYESAMTEA-ESLSATHPIRLGLALNFSV 545
           FY KMKGDY+RY AEFA   +R++ A+ SL AY++A   A   L  THPIRLGLALNFSV
Sbjct: 120 FYYKMKGDYHRYLAEFATGSDRKDAAENSLIAYKAASDIAMNDLPPTHPIRLGLALNFSV 179

Query: 546 FYF 554
           FY+
Sbjct: 180 FYY 182
>sp|P42650|14333_ENTHI 14-3-3 protein 3 (14-3-3-3)
          Length = 236

 Score =  223 bits (569), Expect = 2e-58
 Identities = 113/179 (63%), Positives = 147/179 (82%), Gaps = 1/179 (0%)
 Frame = +3

Query: 21  EENVYRAKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASWRILS 200
           E+ V+ +KLAEQ+ERYDEMV+ MK VAA +TELSVEERNLLSVA+KNVIGSRRASWRI++
Sbjct: 2   EDCVFLSKLAEQSERYDEMVQYMKQVAALNTELSVEERNLLSVAYKNVIGSRRASWRIIT 61

Query: 201 TIEQREEAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVFYLK 380
           ++EQ+E+AKG+ +  +E+ K YR ++E ELA+ CD+V+KV+   LLP AS +ES+VFY K
Sbjct: 62  SLEQKEQAKGNDK-HVEIIKGYRAKIEDELAKYCDDVLKVIKENLLPNASTSESKVFYKK 120

Query: 381 MKGDYYRYRAEFAIEKEREEVAQKSLGAY-ESAMTEAESLSATHPIRLGLALNFSVFYF 554
           M+GDYYRY AEF ++++R+EVA KSL AY E+       L+ THPIRLGLALNFSVFY+
Sbjct: 121 MEGDYYRYYAEFTVDEKRQEVADKSLAAYTEATEISNADLAPTHPIRLGLALNFSVFYY 179
>sp|Q99002|1433_TRIHA 14-3-3 protein homolog (Th1433)
          Length = 262

 Score =  220 bits (560), Expect = 2e-57
 Identities = 111/179 (62%), Positives = 149/179 (83%), Gaps = 1/179 (0%)
 Frame = +3

Query: 21  EENVYRAKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASWRILS 200
           E+ VY AKLAEQAERY+EMV++MK VA+ D +L+VEERNLLSVA+KNVIG+RRASWRI++
Sbjct: 4   EDAVYLAKLAEQAERYEEMVENMKIVASEDRDLTVEERNLLSVAYKNVIGARRASWRIVT 63

Query: 201 TIEQREEAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVFYLK 380
           +IEQ+EE+KG+S  ++ L K YR+++E ELA++CD++++VLD  L+P A + ES+VFY K
Sbjct: 64  SIEQKEESKGNSS-QVTLIKEYRQKIENELAKICDDILEVLDQHLIPSAKSGESKVFYHK 122

Query: 381 MKGDYYRYRAEFAIEKEREEVAQKSLGAYESAMTEAES-LSATHPIRLGLALNFSVFYF 554
           +KGDY+RY AEFAI   R++ A KSL AY++A   A++ L  THPIRLGLALNFSVFY+
Sbjct: 123 IKGDYHRYLAEFAIGDRRKDSADKSLEAYKAATEVAQTELPPTHPIRLGLALNFSVFYY 181
>sp|Q96453|1433D_SOYBN 14-3-3-like protein D (SGF14D)
          Length = 261

 Score =  219 bits (559), Expect = 3e-57
 Identities = 115/181 (63%), Positives = 143/181 (79%), Gaps = 1/181 (0%)
 Frame = +3

Query: 15  DREENVYRAKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASWRI 194
           DRE  VY AKLAEQAERY+EMV+SMK VA  D EL+VEERNLLSV +KNVIG+RRASWRI
Sbjct: 6   DRENFVYIAKLAEQAERYEEMVESMKNVANLDVELTVEERNLLSVGYKNVIGARRASWRI 65

Query: 195 LSTIEQREEAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVFY 374
           LS+IEQ+EE KG +++  +  K YR++VE EL+ +C++V++V+D  L+P A+  ES VFY
Sbjct: 66  LSSIEQKEETKG-NELNAKRIKEYRQKVELELSNICNDVMRVIDEHLIPSAAAGESTVFY 124

Query: 375 LKMKGDYYRYRAEFAIEKEREEVAQKSLGAYESAMTEAES-LSATHPIRLGLALNFSVFY 551
            KMKGDYYRY AEF    E++E A +S+ AYESA   AE+ L  THPIRLGLALNFSVFY
Sbjct: 125 YKMKGDYYRYLAEFKSGNEKKEAADQSMKAYESATAAAEADLPPTHPIRLGLALNFSVFY 184

Query: 552 F 554
           +
Sbjct: 185 Y 185
>sp|P48347|14310_ARATH 14-3-3-like protein GF14 epsilon (General regulatory factor 10)
          Length = 254

 Score =  219 bits (558), Expect = 4e-57
 Identities = 110/181 (60%), Positives = 147/181 (81%), Gaps = 1/181 (0%)
 Frame = +3

Query: 15  DREENVYRAKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASWRI 194
           +RE+ VY AKL+EQ ERYDEMV++MK VA  D EL+VEERNL+SV +KNVIG+RRASWRI
Sbjct: 4   EREKQVYLAKLSEQTERYDEMVEAMKKVAQLDVELTVEERNLVSVGYKNVIGARRASWRI 63

Query: 195 LSTIEQREEAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVFY 374
           LS+IEQ+EE+KG+ +  ++  K YRK+VE ELA+VC++++ V+D  L+P ++  ES VF+
Sbjct: 64  LSSIEQKEESKGNDE-NVKRLKNYRKRVEDELAKVCNDILSVIDKHLIPSSNAVESTVFF 122

Query: 375 LKMKGDYYRYRAEFAIEKEREEVAQKSLGAYESAMTEAES-LSATHPIRLGLALNFSVFY 551
            KMKGDYYRY AEF+   ER+E A +SL AY++A+  AE+ L+ THP+RLGLALNFSVFY
Sbjct: 123 YKMKGDYYRYLAEFSSGAERKEAADQSLEAYKAAVAAAENGLAPTHPVRLGLALNFSVFY 182

Query: 552 F 554
           +
Sbjct: 183 Y 183
>sp|P93212|1437_LYCES 14-3-3 protein 7
          Length = 252

 Score =  219 bits (557), Expect = 5e-57
 Identities = 109/181 (60%), Positives = 143/181 (79%), Gaps = 1/181 (0%)
 Frame = +3

Query: 15  DREENVYRAKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASWRI 194
           +RE+ VY A+LAEQAERYDEMV++MK +A  D EL+VEERNL+SV +KNVIG+RRASWRI
Sbjct: 4   EREKQVYLARLAEQAERYDEMVEAMKAIAKMDVELTVEERNLVSVGYKNVIGARRASWRI 63

Query: 195 LSTIEQREEAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVFY 374
           LS+IEQ+EE+KG  Q  ++  K YR++VE EL ++C +++ V+D  L+P ++  ES VFY
Sbjct: 64  LSSIEQKEESKGHEQ-NVKRIKTYRQRVEDELTKICSDILSVIDEHLVPSSTTGESTVFY 122

Query: 375 LKMKGDYYRYRAEFAIEKEREEVAQKSLGAYESAMTEAES-LSATHPIRLGLALNFSVFY 551
            KMKGDYYRY AEF    +R+E +++SL AYE+A   A S L+ THPIRLGLALNFSVFY
Sbjct: 123 YKMKGDYYRYLAEFKAGDDRKEASEQSLKAYEAATATASSDLAPTHPIRLGLALNFSVFY 182

Query: 552 F 554
           +
Sbjct: 183 Y 183
>sp|P34730|BMH2_YEAST Protein BMH2
          Length = 273

 Score =  218 bits (556), Expect = 6e-57
 Identities = 110/180 (61%), Positives = 145/180 (80%), Gaps = 1/180 (0%)
 Frame = +3

Query: 18  REENVYRAKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASWRIL 197
           RE++VY AKLAEQAERY+EMV++MK VA+S  ELSVEERNLLSVA+KNVIG+RRASWRI+
Sbjct: 5   REDSVYLAKLAEQAERYEEMVENMKAVASSGQELSVEERNLLSVAYKNVIGARRASWRIV 64

Query: 198 STIEQREEAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVFYL 377
           S+IEQ+EE+K  S+ ++EL ++YR ++E EL ++ D+++ VLD+ L+P A+  ES+VFY 
Sbjct: 65  SSIEQKEESKEKSEHQVELIRSYRSKIETELTKISDDILSVLDSHLIPSATTGESKVFYY 124

Query: 378 KMKGDYYRYRAEFAIEKEREEVAQKSLGAYESAMTEAES-LSATHPIRLGLALNFSVFYF 554
           KMKGDY+RY AEF+    RE+    SL AY++A   A + L  THPIRLGLALNFSVFY+
Sbjct: 125 KMKGDYHRYLAEFSSGDAREKATNSSLEAYKTASEIATTELPPTHPIRLGLALNFSVFYY 184
>sp|P93208|14332_LYCES 14-3-3 protein 2
          Length = 254

 Score =  218 bits (556), Expect = 6e-57
 Identities = 111/182 (60%), Positives = 148/182 (81%), Gaps = 3/182 (1%)
 Frame = +3

Query: 18  REENVYRAKLAEQAERYDEMVKSMKCVAAS--DTELSVEERNLLSVAFKNVIGSRRASWR 191
           REENVY AKLAEQAERY+EMV+ M+ V+ S    EL+VEERNLLSVA+KNVIG+RRASWR
Sbjct: 3   REENVYMAKLAEQAERYEEMVQFMEKVSTSLGSEELTVEERNLLSVAYKNVIGARRASWR 62

Query: 192 ILSTIEQREEAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVF 371
           I+S+IEQ+EE++G+ +  ++  K YR ++E EL+++CD ++K+LD+ L+P ASN +S+VF
Sbjct: 63  IISSIEQKEESRGNEE-HVKCIKEYRSKIESELSDICDGILKLLDSNLIPSASNGDSKVF 121

Query: 372 YLKMKGDYYRYRAEFAIEKEREEVAQKSLGAYESAMTEAES-LSATHPIRLGLALNFSVF 548
           YLKMKGDY+RY AEF    ER+E A+ +L AY++A   A + L+ THPIRLGLALNFSVF
Sbjct: 122 YLKMKGDYHRYLAEFKTGAERKEAAESTLSAYKAAQDIANTELAPTHPIRLGLALNFSVF 181

Query: 549 YF 554
           Y+
Sbjct: 182 YY 183
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57,401,276
Number of Sequences: 369166
Number of extensions: 1016321
Number of successful extensions: 3757
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3431
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3501
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 3931403200
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)