Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_024_O03
(805 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P35603|AP50_CAEEL Clathrin coat assembly protein AP50 (C... 70 9e-12
sp|P84092|AP2M1_RAT Clathrin coat assembly protein AP50 (Cl... 67 4e-11
sp|P54672|AP50_DICDI Clathrin coat assembly protein AP50 (C... 49 2e-05
sp|Q9BXS5|AP1M1_HUMAN Adaptor-related protein complex 1, mu... 45 2e-04
sp|P35585|AP1M1_MOUSE Adaptor-related protein complex 1, mu... 45 2e-04
sp|Q00776|AP54_YEAST Clathrin coat assembly protein AP54 (C... 44 5e-04
sp|P35602|AP47_CAEEL Clathrin coat assembly protein AP47 (C... 39 0.022
sp|Q9Y2T2|AP3M1_HUMAN Adapter-related protein complex 3 mu ... 38 0.037
sp|Q9JKC8|AP3M1_MOUSE Adapter-related protein complex 3 mu ... 38 0.037
sp|P53676|AP3M1_RAT Adapter-related protein complex 3 mu 1 ... 38 0.037
>sp|P35603|AP50_CAEEL Clathrin coat assembly protein AP50 (Clathrin coat-associated
protein AP50) (Plasma membrane adaptor AP-2 50 kDa
protein) (Clathrin assembly protein complex 2 medium
chain) (Dumpy protein 23)
Length = 441
Score = 69.7 bits (169), Expect = 9e-12
Identities = 30/42 (71%), Positives = 39/42 (92%)
Frame = +3
Query: 96 RVNVIHA*QQVRNPVTSIARTSFFHVKQGSIWLCAVAKTDVN 221
RVNVIHA QQVR+PVT++ARTSFFHVK+G++W+CAV + +VN
Sbjct: 33 RVNVIHARQQVRSPVTNMARTSFFHVKRGNVWICAVTRQNVN 74
Score = 47.0 bits (110), Expect = 6e-05
Identities = 21/40 (52%), Positives = 27/40 (67%)
Frame = +2
Query: 230 FQFLHRLFDIMEQYFDTVNEGKIKNNFIHIYELLDGNWEF 349
F+FL R D M+ YF +NE +KNNF+ IYELLD +F
Sbjct: 79 FEFLKRFADTMQSYFGKLNEENVKNNFVLIYELLDEILDF 118
>sp|P84092|AP2M1_RAT Clathrin coat assembly protein AP50 (Clathrin coat-associated
protein AP50) (Plasma membrane adaptor AP-2 50 kDa
protein) (Clathrin assembly protein complex 2 medium
chain) (AP-2 mu 2 chain)
sp|P84091|AP2M1_MOUSE Clathrin coat assembly protein AP50 (Clathrin coat-associated
protein AP50) (Plasma membrane adaptor AP-2 50 kDa
protein) (Clathrin assembly protein complex 2 medium
chain) (AP-2 mu 2 chain)
sp|Q96CW1|AP2M1_HUMAN Clathrin coat assembly protein AP50 (Clathrin coat-associated
protein AP50) (Plasma membrane adaptor AP-2 50 kDa
protein) (HA2 50 kDa subunit) (Clathrin assembly protein
complex 2 medium chain) (AP-2 mu 2 chain)
Length = 435
Score = 67.4 bits (163), Expect = 4e-11
Identities = 32/42 (76%), Positives = 37/42 (88%)
Frame = +3
Query: 96 RVNVIHA*QQVRNPVTSIARTSFFHVKQGSIWLCAVAKTDVN 221
RVNVIHA QQVR+PVT+IARTSFFHVK+ +IWL AV K +VN
Sbjct: 33 RVNVIHARQQVRSPVTNIARTSFFHVKRSNIWLAAVTKQNVN 74
Score = 47.8 bits (112), Expect = 4e-05
Identities = 24/55 (43%), Positives = 35/55 (63%)
Frame = +2
Query: 185 YLALCSGQN*C*YCEFQFLHRLFDIMEQYFDTVNEGKIKNNFIHIYELLDGNWEF 349
+LA + QN F+FL+++ D+M YF ++E IKNNF+ IYELLD +F
Sbjct: 64 WLAAVTKQNVNAAMVFEFLYKMCDVMAAYFGKISEENIKNNFVLIYELLDEILDF 118
>sp|P54672|AP50_DICDI Clathrin coat assembly protein AP50 (Clathrin coat-associated
protein AP50) (Plasma membrane adaptor AP-2 50 kDa
protein) (Clathrin assembly protein complex 2 medium
chain)
Length = 439
Score = 48.5 bits (114), Expect = 2e-05
Identities = 24/55 (43%), Positives = 34/55 (61%)
Frame = +2
Query: 185 YLALCSGQN*C*YCEFQFLHRLFDIMEQYFDTVNEGKIKNNFIHIYELLDGNWEF 349
Y+ S QN F+ LH+L DI + YFD ++E I+NNF+ +YELLD +F
Sbjct: 63 YIVGVSRQNVNACMVFEVLHQLVDIFKSYFDNLDEDSIRNNFVLVYELLDEILDF 117
Score = 34.7 bits (78), Expect = 0.31
Identities = 14/34 (41%), Positives = 24/34 (70%)
Frame = +3
Query: 120 QQVRNPVTSIARTSFFHVKQGSIWLCAVAKTDVN 221
Q+ R+PV I TSF ++K G+I++ V++ +VN
Sbjct: 40 QETRSPVKLIGSTSFMYIKVGNIYIVGVSRQNVN 73
>sp|Q9BXS5|AP1M1_HUMAN Adaptor-related protein complex 1, mu 1 subunit (Mu-adaptin 1)
(Adaptor protein complex AP-1 mu-1 subunit) (Golgi
adaptor HA1/AP1 adaptin mu-1 subunit) (Clathrin assembly
protein assembly protein complex 1 medium chain 1)
(Clathrin coat assembly protein AP47) (Clathrin
coat-associated protein AP47) (AP-mu chain family member
mu1A)
Length = 423
Score = 45.1 bits (105), Expect = 2e-04
Identities = 21/55 (38%), Positives = 32/55 (58%)
Frame = +2
Query: 185 YLALCSGQN*C*YCEFQFLHRLFDIMEQYFDTVNEGKIKNNFIHIYELLDGNWEF 349
YL S +N C F FL+++ + +YF + E I++NF+ IYELLD +F
Sbjct: 66 YLVATSKKNACVSLVFSFLYKVVQVFSEYFKELEEESIRDNFVIIYELLDELMDF 120
>sp|P35585|AP1M1_MOUSE Adaptor-related protein complex 1, mu 1 subunit (Mu-adaptin 1)
(Adaptor protein complex AP-1 mu-1 subunit) (Golgi
adaptor HA1/AP1 adaptin mu-1 subunit) (Clathrin assembly
protein assembly protein complex 1 medium chain 1)
(Clathrin coat assembly protein AP47) (Clathrin
coat-associated protein AP47) (AP-mu chain family member
mu1A)
Length = 423
Score = 45.1 bits (105), Expect = 2e-04
Identities = 21/55 (38%), Positives = 32/55 (58%)
Frame = +2
Query: 185 YLALCSGQN*C*YCEFQFLHRLFDIMEQYFDTVNEGKIKNNFIHIYELLDGNWEF 349
YL S +N C F FL+++ + +YF + E I++NF+ IYELLD +F
Sbjct: 66 YLVATSKKNACVSLVFSFLYKVVQVFSEYFKELEEESIRDNFVIIYELLDELMDF 120
>sp|Q00776|AP54_YEAST Clathrin coat assembly protein AP54 (Clathrin coat-associated
protein AP54) (Golgi adaptor AP-1 54 kDa protein) (HA1
54 kDa subunit) (Clathrin assembly protein complex 1
medium chain)
Length = 475
Score = 43.9 bits (102), Expect = 5e-04
Identities = 18/35 (51%), Positives = 25/35 (71%)
Frame = +2
Query: 230 FQFLHRLFDIMEQYFDTVNEGKIKNNFIHIYELLD 334
F FLH+L +++ Y TV E I++NF+ IYELLD
Sbjct: 82 FTFLHKLVEVLSDYLKTVEEESIRDNFVIIYELLD 116
>sp|P35602|AP47_CAEEL Clathrin coat assembly protein AP47 (Clathrin coat-associated
protein AP47) (Golgi adaptor AP-1 47 kDa protein) (HA1
47 kDa subunit) (Clathrin assembly protein assembly
protein complex 1 medium chain) (Uncoordinated protein
101)
Length = 422
Score = 38.5 bits (88), Expect = 0.022
Identities = 15/38 (39%), Positives = 25/38 (65%)
Frame = +2
Query: 236 FLHRLFDIMEQYFDTVNEGKIKNNFIHIYELLDGNWEF 349
FL++ ++ +YF V E +++NF+ IYELLD +F
Sbjct: 83 FLYKCVEVFSEYFKDVEEESVRDNFVVIYELLDEMMDF 120
>sp|Q9Y2T2|AP3M1_HUMAN Adapter-related protein complex 3 mu 1 subunit (Mu-adaptin 3A)
(AP-3 adapter complex mu3A subunit)
Length = 418
Score = 37.7 bits (86), Expect = 0.037
Identities = 15/34 (44%), Positives = 23/34 (67%)
Frame = +2
Query: 233 QFLHRLFDIMEQYFDTVNEGKIKNNFIHIYELLD 334
+FLHR+ D + YF +E IK+N + +YELL+
Sbjct: 81 EFLHRVADTFQDYFGECSEAAIKDNVVIVYELLE 114
>sp|Q9JKC8|AP3M1_MOUSE Adapter-related protein complex 3 mu 1 subunit (Mu-adaptin 3A)
(AP-3 adapter complex mu3A subunit)
Length = 418
Score = 37.7 bits (86), Expect = 0.037
Identities = 15/34 (44%), Positives = 23/34 (67%)
Frame = +2
Query: 233 QFLHRLFDIMEQYFDTVNEGKIKNNFIHIYELLD 334
+FLHR+ D + YF +E IK+N + +YELL+
Sbjct: 81 EFLHRVADTFQDYFGECSEAAIKDNVVIVYELLE 114
>sp|P53676|AP3M1_RAT Adapter-related protein complex 3 mu 1 subunit (Mu-adaptin 3A)
(AP-3 adapter complex mu3A subunit) (Clathrin coat
assembly protein AP47 homolog 1) (Clathrin
coat-associated protein AP47 homolog 1) (Golgi adaptor
AP-1 47 kDa protein homolog 1) (HA1 47 kDa subunit
homolog 1) (Clathrin assembly protein assembly protein
complex 1 medium chain homolog 1) (P47A)
Length = 418
Score = 37.7 bits (86), Expect = 0.037
Identities = 15/34 (44%), Positives = 23/34 (67%)
Frame = +2
Query: 233 QFLHRLFDIMEQYFDTVNEGKIKNNFIHIYELLD 334
+FLHR+ D + YF +E IK+N + +YELL+
Sbjct: 81 EFLHRVADTFQDYFGECSEAAIKDNVVIVYELLE 114
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,398,323
Number of Sequences: 369166
Number of extensions: 1636860
Number of successful extensions: 3216
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3065
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3198
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7618580670
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)