Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_024_N12
(801 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P04190|BLA2_BACCE Beta-lactamase II precursor (Penicilli... 60 5e-09
sp|P14488|BLAB_BACCE Beta-lactamase II precursor (Penicilli... 59 2e-08
sp|P10425|BLA2_BAC17 Beta-lactamase II precursor (Penicilli... 53 1e-06
sp|P25910|BLAB_BACFR Beta-lactamase type II precursor (Peni... 51 3e-06
sp|Q08889|GLO2_BUCAP Probable hydroxyacylglutathione hydrol... 50 5e-06
sp|P57336|GLO2_BUCAI Probable hydroxyacylglutathione hydrol... 48 3e-05
sp|P54435|YRKH_BACSU Hypothetical protein yrkH 47 5e-05
sp|P75849|YCBL_ECOLI Hypothetical protein ycbL 46 1e-04
sp|Q05584|GLO2_YEAST Hydroxyacylglutathione hydrolase, cyto... 46 1e-04
sp|Q89AN4|GLO2_BUCBP Probable hydroxyacylglutathione hydrol... 46 1e-04
>sp|P04190|BLA2_BACCE Beta-lactamase II precursor (Penicillinase) (Cephalosporinase)
Length = 257
Score = 60.5 bits (145), Expect = 5e-09
Identities = 58/226 (25%), Positives = 103/226 (45%), Gaps = 16/226 (7%)
Frame = +3
Query: 51 DKTMHK*NIMLPSIPKISQIVPGITRILGMNPSAMTLQGTNSYLIGTNTSKLLIDTSNLH 230
+KT+ K SI ++++ V T + N A+ +N ++ T+ +L+D+S
Sbjct: 35 EKTVIKNETGTISISQLNKNVWVHTELGSFNGEAVP---SNGLVLNTSKGLVLVDSS--- 88
Query: 231 QNAYIDNLRQYLIESCENS---TISDIIVTHWHPDHIGGVSDI-------FDLAKSLNWK 380
+ D L + LIE E ++D+I+TH H D IGG+ + A +
Sbjct: 89 ---WDDKLTKELIEMVEKKFQKRVTDVIITHAHADRIGGIKTLKERGIKAHSTALTAELA 145
Query: 381 KPNVFKYPSLDHSSECNILKSDEVISLSKYGMGTLKVLYT-PGHTDDHICLLLQPENILF 557
K N ++ P D + N+ K+G ++ Y GHT+D+I + L NIL
Sbjct: 146 KKNGYEEPLGDLQTVTNL----------KFGNMKVETFYPGKGHTEDNIVVWLPQYNILV 195
Query: 558 SGDLILGNGSTTFENLT-LYMN----SLKTVESIGNSLSMILPGHG 680
G L+ + N+ Y+N S++ V +++ ++PGHG
Sbjct: 196 GGCLVKSTSAKDLGNVADAYVNEWSTSIENVLKRYRNINAVVPGHG 241
>sp|P14488|BLAB_BACCE Beta-lactamase II precursor (Penicillinase) (Cephalosporinase)
Length = 256
Score = 58.5 bits (140), Expect = 2e-08
Identities = 50/187 (26%), Positives = 86/187 (45%), Gaps = 16/187 (8%)
Frame = +3
Query: 168 TNSYLIGTNTSKLLIDTSNLHQNAYIDNLRQYLIESCENS---TISDIIVTHWHPDHIGG 338
+N ++ T+ +L+D+S + D L + LIE E ++D+I+TH H D IGG
Sbjct: 70 SNGLVLNTSKGLVLVDSS------WDDKLTKELIEMVEKKFKKRVTDVIITHAHADRIGG 123
Query: 339 VSDI-------FDLAKSLNWKKPNVFKYPSLDHSSECNILKSDEVISLSKYGMGTLKVLY 497
+ + A + K N ++ P D S N+ K+G ++ Y
Sbjct: 124 MKTLKERGIKAHSTALTAELAKKNGYEEPLGDLQSVTNL----------KFGNMKVETFY 173
Query: 498 T-PGHTDDHICLLLQPENILFSGDLILGNGSTTFENLT-LYMN----SLKTVESIGNSLS 659
GHT+D+I + L IL G L+ S N+ Y+N S++ V +++
Sbjct: 174 PGKGHTEDNIVVWLPQYQILAGGCLVKSASSKDLGNVADAYVNEWSTSIENVLKRYGNIN 233
Query: 660 MILPGHG 680
+++PGHG
Sbjct: 234 LVVPGHG 240
>sp|P10425|BLA2_BAC17 Beta-lactamase II precursor (Penicillinase)
Length = 257
Score = 52.8 bits (125), Expect = 1e-06
Identities = 46/189 (24%), Positives = 88/189 (46%), Gaps = 18/189 (9%)
Frame = +3
Query: 168 TNSYLIGTNTSKLLIDTSNLHQNAYIDNLRQYLIESCENS---TISDIIVTHWHPDHIGG 338
+N ++ T+ +L+D+S + + L + LIE E ++D+I+TH H D IGG
Sbjct: 71 SNGLVLNTSKGLVLVDSS------WDNKLTKELIEMVEKKFQKRVTDVIITHAHADRIGG 124
Query: 339 VSDI-------FDLAKSLNWKKPNVFKYPSLDHSSECNILKSDEVISLSKYGMGTLKVLY 497
++ + A + K + ++ P D + N+ K+G ++ Y
Sbjct: 125 ITALKERGIKAHSTALTAELAKKSGYEEPLGDLQTVTNL----------KFGNTKVETFY 174
Query: 498 T-PGHTDDHICLLLQPENILFSGDLI-------LGNGSTTFENLTLYMNSLKTVESIGNS 653
GHT+D+I + L IL G L+ LGN + + N + S++ + +
Sbjct: 175 PGKGHTEDNIVVWLPQYQILAGGCLVKSAEAKNLGNVADAYVN--EWSTSIENMLKRYRN 232
Query: 654 LSMILPGHG 680
+++++PGHG
Sbjct: 233 INLVVPGHG 241
>sp|P25910|BLAB_BACFR Beta-lactamase type II precursor (Penicillinase) (Cephalosporinase)
(Imipenem-cefoxitin hydrolyzing enzyme)
Length = 249
Score = 51.2 bits (121), Expect = 3e-06
Identities = 48/189 (25%), Positives = 82/189 (43%), Gaps = 18/189 (9%)
Frame = +3
Query: 168 TNSYLIGTNTSKLLIDTSNLHQNAYIDNLRQYLIESCENSTISDIIVTHWHPDHIGGVSD 347
+N ++ N L+DT +A + L ++ +S ++ ++ I HWH D IGG+
Sbjct: 54 SNGMIVINNHQAALLDTPI--NDAQTEMLVNWVTDSL-HAKVTTFIPNHWHGDCIGGLGY 110
Query: 348 I-------------FDLAKSLNWKKPNVFKYPSLDHSSECNILKSDEVISLSKYGMGTLK 488
+ DLAK P +H ++ S + + L Y +G
Sbjct: 111 LQRKGVQSYANQMTIDLAKEKGLPVP--------EHGFTDSLTVSLDGMPLQCYYLGG-- 160
Query: 489 VLYTPGHTDDHICLLLQPENILFSGDLILGNGSTTFENL-----TLYMNSLKTVESIGNS 653
GH D+I + L ENILF G ++ N +T+ N+ T + +L V++ S
Sbjct: 161 -----GHATDNIVVWLPTENILFGGCMLKDNQATSIGNISDADVTAWPKTLDKVKAKFPS 215
Query: 654 LSMILPGHG 680
++PGHG
Sbjct: 216 ARYVVPGHG 224
>sp|Q08889|GLO2_BUCAP Probable hydroxyacylglutathione hydrolase (Glyoxalase II) (Glx II)
Length = 250
Score = 50.4 bits (119), Expect = 5e-06
Identities = 50/177 (28%), Positives = 80/177 (45%), Gaps = 12/177 (6%)
Frame = +3
Query: 279 ENSTISDIIVTHWHPDHIGGVSDIFDLAKSLNWKKPNVFKY-PSLDHSSECN-ILKSDEV 452
+N + I++TH H DH+GGV +I + PNV + P + N I+K +V
Sbjct: 42 KNWKLIAILLTHNHIDHVGGVEEIIR-------RYPNVTVFGPEETKTRNVNKIVKQGDV 94
Query: 453 ISLSKYGMGTLKVLYTPGHTDDHICLLLQPENILFSGDLILGNG-STTFENLTL------ 611
I L K + V TPGHT H+ L+P +F GD + G F+N
Sbjct: 95 IKLLK---SEIHVFLTPGHTLGHVSYYLKP--YIFCGDTLFSGGCGRVFKNKFFDMYQSI 149
Query: 612 -YMNSL--KTVESIGNSLSMILPGHGVEIDQPVSKIKEYLQHRQERIKQVFNALEIS 773
++ SL KT+ + ++ + I KIK+Y + ++ I Q +L +S
Sbjct: 150 NFIKSLPKKTILCCSHEYTLSNLNFAMSILPFDKKIKKYYKKIKKHISQNKTSLPVS 206
>sp|P57336|GLO2_BUCAI Probable hydroxyacylglutathione hydrolase (Glyoxalase II) (GLX II)
Length = 251
Score = 48.1 bits (113), Expect = 3e-05
Identities = 44/162 (27%), Positives = 69/162 (42%), Gaps = 4/162 (2%)
Frame = +3
Query: 180 LIGTNTSKLLIDTSNLHQNAYIDNLRQYLIESCENST--ISDIIVTHWHPDHIGGVSDIF 353
L+ T+ S ++ID L + +I+ E + I++TH H DH GG I
Sbjct: 17 LLNTSGSCIIIDPG----------LSEPIIQEIERKKWRLRAILLTHNHIDHTGGTRKII 66
Query: 354 DLAKSLNWKKPNVFKYPSLDHSSECNILKSDEVISLSKYGMGTLKVLYTPGHTDDHICLL 533
+ ++ P + H + D +I L K V +TPGHT H+
Sbjct: 67 EYFPKISVFGPKETR----QHGVNKIVSHGDRIILLDKI----FYVFFTPGHTSGHVSYY 118
Query: 534 LQPENILFSGDLILGNG-STTFENLTLYM-NSLKTVESIGNS 653
QP +F GD + G F+N L M S+K + S+ +S
Sbjct: 119 SQP--YIFCGDTLFSAGCGRVFKNKHLEMYRSIKIISSLPDS 158
>sp|P54435|YRKH_BACSU Hypothetical protein yrkH
Length = 295
Score = 47.4 bits (111), Expect = 5e-05
Identities = 47/187 (25%), Positives = 86/187 (45%), Gaps = 9/187 (4%)
Frame = +3
Query: 162 QGTNSYLIGTNTSKLLIDTSNLHQNAYIDNLRQYLIESCENSTISDIIVTHWHPDHIGGV 341
+G SY++ +N +ID + + N +ID + + +TI+ + TH H DHI G
Sbjct: 49 KGCLSYMVISNGEAAIIDATRM-VNFFIDFAKG------KGATITHVFDTHLHADHISGG 101
Query: 342 SDIFDLAKSLNWKKPNVFKYPSLDHSSECNILKSDEVISLSKYGMGTLKV--LYTPGHTD 515
I + K+ W P + D+ + + +++I G T+K+ +Y+PGHT
Sbjct: 102 RVIAEKTKATYWLPPKDAEEVEFDYQP---LEEGNDII----IGNTTIKIQPIYSPGHTI 154
Query: 516 DHICLLLQPENILFSGDLI----LGNGSTTFENLTLYMNSLKTVESIGNSLS---MILPG 674
++ + L SGD++ +G + KT+ S +LS ++LP
Sbjct: 155 GSTSFIVD-DQYLLSGDILFIDSIGRPDLAGMAEDWVGDLRKTLYSRYKALSKDLIVLPA 213
Query: 675 HGVEIDQ 695
H + ID+
Sbjct: 214 HFMIIDE 220
>sp|P75849|YCBL_ECOLI Hypothetical protein ycbL
Length = 215
Score = 46.2 bits (108), Expect = 1e-04
Identities = 40/147 (27%), Positives = 66/147 (44%), Gaps = 16/147 (10%)
Frame = +3
Query: 288 TISDIIVTHWHPDHIGGVSDIFDL--AKSLNWKKPNVFKYPSLDHSS------ECNILKS 443
T+ I++TH H DH+G +++ +K + F L S EC L
Sbjct: 48 TLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEECQPLTP 107
Query: 444 DEVISLS---KYGMGTLKVLYTPGHTDDHICLLLQPENILFSGDLIL--GNGSTTF---E 599
D ++ G TL+VL+ PGHT H+ +L SGD+I G G + F +
Sbjct: 108 DRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGRSDFPRGD 167
Query: 600 NLTLYMNSLKTVESIGNSLSMILPGHG 680
+ L + + +G+ + + +PGHG
Sbjct: 168 HNQLISSIKDKLLPLGDDV-IFIPGHG 193
>sp|Q05584|GLO2_YEAST Hydroxyacylglutathione hydrolase, cytoplasmic isozyme (Glyoxalase
II) (Glx II)
Length = 274
Score = 46.2 bits (108), Expect = 1e-04
Identities = 56/184 (30%), Positives = 83/184 (45%), Gaps = 20/184 (10%)
Frame = +3
Query: 186 GTNTSKLLIDTSNLHQNAYIDNLR--QYLIESCENSTIS--DIIVTHWHPDHIGGVSDIF 353
G N LL D+ N ++ ID + L E E+ IS I+ TH H DH G +DI
Sbjct: 14 GVNYCYLLSDSKN-KKSWLIDPAEPPEVLPELTEDEKISVEAIVNTHHHYDHADGNADIL 72
Query: 354 DLAKSLNWKKPNVFKYPSLDHSSEC---NILKSDEVISLSKYGMGTLKV--LYTPGHTDD 518
K L K P K + S +C I+ +L K +G L++ + TP HT D
Sbjct: 73 ---KYLKEKNP-TSKVEVIGGSKDCPKVTIIPE----NLKKLHLGDLEITCIRTPCHTRD 124
Query: 519 HICLLLQ----PENILFSGDLILGNGSTTF-----ENLTLYMNS--LKTVESIGNSLSMI 665
IC ++ E +F+GD + G F E + + +N+ L+TV S + +
Sbjct: 125 SICYYVKDPTTDERCIFTGDTLFTAGCGRFFEGTGEEMDIALNNSILETVGRQNWSKTRV 184
Query: 666 LPGH 677
PGH
Sbjct: 185 YPGH 188
>sp|Q89AN4|GLO2_BUCBP Probable hydroxyacylglutathione hydrolase (Glyoxalase II) (Glx II)
Length = 249
Score = 45.8 bits (107), Expect = 1e-04
Identities = 43/156 (27%), Positives = 66/156 (42%), Gaps = 4/156 (2%)
Frame = +3
Query: 300 IIVTHWHPDHIGGVSDIFDLAKSLNWKKPNVFKYPSLD--HSSECNILKSDEVISLSKYG 473
I++TH H DHI GV K+L +K P + Y L+ N++ + I +
Sbjct: 49 ILLTHNHLDHIQGV-------KNLLYKYPEISIYGPLETQFCGTKNLIDNRNTIEILN-- 99
Query: 474 MGTLKVLYTPGHTDDHICLLLQPENILFSGDLILGNGSTTFEN--LTLYMNSLKTVESIG 647
++ TPGHT+ H P LF GD I G +N + NSL+T+ +
Sbjct: 100 -TKFYIIPTPGHTNGHTAYYCPP--FLFCGDSIFSGGCGRVKNGMMIKMYNSLRTISKLP 156
Query: 648 NSLSMILPGHGVEIDQPVSKIKEYLQHRQERIKQVF 755
N ++I H EY + E K++F
Sbjct: 157 ND-TLIYCSH------------EYTRSNLEFFKKIF 179
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 83,973,326
Number of Sequences: 369166
Number of extensions: 1697448
Number of successful extensions: 4479
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4315
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4456
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7570361805
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)