Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_024_N04
(869 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O13035|SAP_CHICK Proactivator polypeptide precursor [Con... 74 7e-13
sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Con... 70 1e-11
sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Con... 69 2e-11
sp|Q61207|SAP_MOUSE Sulfated glycoprotein 1 precursor (SGP-... 54 7e-07
sp|P10960|SAP_RAT Sulfated glycoprotein 1 precursor (SGP-1)... 51 4e-06
sp|P20097|SAP_CAVPO Saposin C (CO-beta-glucosidase) (Glucos... 49 1e-05
sp|P42211|ASPRX_ORYSA Aspartic proteinase precursor 47 5e-05
sp|P50405|PSPB_MOUSE Pulmonary surfactant-associated protei... 44 4e-04
sp|P07988|PSPB_HUMAN Pulmonary surfactant-associated protei... 42 0.002
sp|P17129|PSPB_CANFA Pulmonary surfactant-associated protei... 42 0.003
>sp|O13035|SAP_CHICK Proactivator polypeptide precursor [Contains: Saposin A; Saposin B;
Saposin C; Saposin D]
Length = 518
Score = 73.6 bits (179), Expect = 7e-13
Identities = 66/307 (21%), Positives = 127/307 (41%), Gaps = 37/307 (12%)
Frame = +1
Query: 28 QLKPIRKMKGIE--CDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQ-CQQ 198
Q KP +K K E C C +V +E + AT + + C G C+
Sbjct: 183 QDKPKQKSKATEDVCQDCIRLVTDVQEAVRTNATFVKSLVAHAKEECDRLGPGMSDMCKS 242
Query: 199 IVDEYFNLILNFILAEVDVDK--ICKFFGLC------------------NAKLSGIE--- 309
+ EY +L + ++ D IC G C K+ +E
Sbjct: 243 YISEYSDLAIQMMMHMKDQQPKDICAMVGFCPSVKSVPLQTLVPAQVVHEVKMETVEKAT 302
Query: 310 --------CELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRLSVIKNECVIIVDTYLP 465
CE+CE ++ EV ++ SN +EE + + +++ + +K++C ++ Y
Sbjct: 303 VQEKTFSVCEICETMVKEVTGLLESNKTEEEIVHEMEVVCYLLPASVKDQCKDFIEVYGQ 362
Query: 466 VIFSIARSTLNSKVICTELKLCTLLSKKKISFLMNRSGNETLKCEICRFAILEVRTLLTD 645
+ + N + +C LK C + ++ +G C+IC+ + L
Sbjct: 363 ALIDMLLEATNPEAVCVMLKCCAANKPPQQPVVVKPAGG---FCDICKMIVAYADKELEK 419
Query: 646 PGFQKIFENFLINEICPHF--KTKQQQCTNDINQYYSLVVNIIVEFLNPS-LCSELGLCN 816
E L+ ++C HF ++ QC + QY +VV ++ E ++P+ +C++LG+C
Sbjct: 420 NATTTEIE-ALLEKVC-HFLPESVSDQCVQFVEQYEPVVVQLLAEMMDPTFVCTKLGVCG 477
Query: 817 STIKQVI 837
+ K ++
Sbjct: 478 AAKKPLL 484
Score = 51.6 bits (122), Expect = 3e-06
Identities = 29/87 (33%), Positives = 52/87 (59%), Gaps = 3/87 (3%)
Frame = +1
Query: 295 LSGIECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL--SVIKNECVIIVDTYLPV 468
++ I C+LC+ ++ V KV+ N +E+ + ++++ K C L + +EC IVD+YLPV
Sbjct: 59 VNSIPCDLCKELVTVVGKVLKDNGTEDEIRSYLE-KRCEFLPDQGLASECKEIVDSYLPV 117
Query: 469 IFSIARSTLNS-KVICTELKLCTLLSK 546
I + + + +V+C+ L LC L K
Sbjct: 118 IMDMIKEEFDKPEVVCSALSLCQSLQK 144
Score = 44.7 bits (104), Expect = 3e-04
Identities = 28/93 (30%), Positives = 47/93 (50%), Gaps = 3/93 (3%)
Frame = +1
Query: 22 NVQLKPIRKMKGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKI--QCQ 195
NV KP + I CD C+ +V + + D TE EI+++L+ C + +C+
Sbjct: 52 NVWSKPA--VNSIPCDLCKELVTVVGKVLKDNGTEDEIRSYLEKR-CEFLPDQGLASECK 108
Query: 196 QIVDEYFNLILNFILAEVD-VDKICKFFGLCNA 291
+IVD Y +I++ I E D + +C LC +
Sbjct: 109 EIVDSYLPVIMDMIKEEFDKPEVVCSALSLCQS 141
>sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B-Val; Saposin B (Sphingolipid activator
protein 1) (SAP-1) (Cerebroside sulfate activator)
(CSAct) (Dispersin) (Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 524
Score = 69.7 bits (169), Expect = 1e-11
Identities = 51/186 (27%), Positives = 96/186 (51%), Gaps = 6/186 (3%)
Frame = +1
Query: 298 SGIECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL-SVIKNECVIIVDTYLPVIF 474
S + CE+CE ++ EV K++ +N +E+ + + K+C +L + EC +VDTY I
Sbjct: 311 SDVYCEVCEFLVKEVTKLIDNNKTEKEILDAFD-KMCSKLPKSLSEECQEVVDTYGSSIL 369
Query: 475 SIARSTLNSKVICTELKLC--TLLSKKKISFLMNRSGNETLKCEICRFAILEV-RTLLTD 645
SI ++ +++C+ L LC T L + + G CE+C+ + + R L +
Sbjct: 370 SILLEEVSPELVCSMLHLCSGTRLPALTVHVTQPKDGG---FCEVCKKLVGYLDRNLEKN 426
Query: 646 PGFQKIFENFLINEICPHFKTK-QQQCTNDINQYYSLVVNIIVEFLNPS-LCSELGLCNS 819
Q+I + + C Q+QC + +Y +++ I+VE ++PS +C ++G C S
Sbjct: 427 STKQEILA--ALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPS 484
Query: 820 TIKQVI 837
K ++
Sbjct: 485 AHKPLL 490
Score = 58.2 bits (139), Expect = 3e-08
Identities = 42/185 (22%), Positives = 84/185 (45%), Gaps = 18/185 (9%)
Frame = +1
Query: 31 LKPIRKMK-----GIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQCQ 195
++PI+K + + C+ C +VK+ + I + TE EI + + S +CQ
Sbjct: 299 VEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKSLSEECQ 358
Query: 196 QIVDEYFNLILNFILAEVDVDKICKFFGLCNA------------KLSGIECELCEHVMDE 339
++VD Y + IL+ +L EV + +C LC+ G CE+C+ ++
Sbjct: 359 EVVDTYGSSILSILLEEVSPELVCSMLHLCSGTRLPALTVHVTQPKDGGFCEVCKKLVGY 418
Query: 340 VIKVVTSNHSEEVVSNFIKLKICPRL-SVIKNECVIIVDTYLPVIFSIARSTLNSKVICT 516
+ + + N +++ + ++ K C L + +C V Y PV+ I ++ +C
Sbjct: 419 LDRNLEKNSTKQEILAALE-KGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCL 477
Query: 517 ELKLC 531
++ C
Sbjct: 478 KIGAC 482
Score = 43.1 bits (100), Expect = 0.001
Identities = 25/83 (30%), Positives = 43/83 (51%), Gaps = 2/83 (2%)
Frame = +1
Query: 49 MKGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSD-KIQCQQIVDEYFNLI 225
+K + CD C+ VV + + D ATE EI +L+ ++ + C++IVD Y +I
Sbjct: 58 VKSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVI 117
Query: 226 LNFILAEVD-VDKICKFFGLCNA 291
L+ I E+ ++C LC +
Sbjct: 118 LDIIKGEMSRPGEVCSALNLCES 140
Score = 42.4 bits (98), Expect = 0.002
Identities = 47/228 (20%), Positives = 97/228 (42%), Gaps = 45/228 (19%)
Frame = +1
Query: 295 LSGIECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRLSV--IKNECVIIVDTYLPV 468
+ + C++C+ V+ ++ N +EE + +++ K C L + C IVD+YLPV
Sbjct: 58 VKSLPCDICKDVVTAAGDMLKDNATEEEILVYLE-KTCDWLPKPNMSASCKEIVDSYLPV 116
Query: 469 IFSIARSTLNSK-VICTELKLCTLLSKKKISFLMNRSGNETLK----------------- 594
I I + ++ +C+ L LC L +K ++ L ++ E+ K
Sbjct: 117 ILDIIKGEMSRPGEVCSALNLCESL-QKHLAELNHQKQLESNKIPELDMTEVVAPFMANI 175
Query: 595 ----------------------CEICRFAILEVRTLL-TDPGF-QKIFENFLINEICPHF 702
C+ C + +++T + T+ F Q + E+ + E C
Sbjct: 176 PLLLYPQDGPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTFVQALVEH--VKEECDRL 233
Query: 703 KTKQQQ-CTNDINQYYSLVVNIIVEFLNPSLCSELGLCNSTIKQVIMR 843
C N I+QY + + +++ +C+ +G C+ +K++ M+
Sbjct: 234 GPGMADICKNYISQYSEIAIQMMMHMQPKEICALVGFCDE-VKEMPMQ 280
>sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B (Sphingolipid activator protein 1) (SAP-1)
(Cerebroside sulfate activator) (CSAct) (Dispersin)
(Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 525
Score = 68.9 bits (167), Expect = 2e-11
Identities = 48/184 (26%), Positives = 98/184 (53%), Gaps = 6/184 (3%)
Frame = +1
Query: 304 IECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL-SVIKNECVIIVDTYLPVIFSI 480
I CE+CE V+ EV K++ +N +EE + + + K+C +L + + +C +VDTY I SI
Sbjct: 314 IYCEVCEFVVKEVAKLIDNNRTEEEILHALD-KVCSKLPTSLAEQCQEVVDTYGRSILSI 372
Query: 481 ARSTLNSKVICTELKLCTL--LSKKKISFLMNRSGNETLKCEICRFAILEV-RTLLTDPG 651
+ +++C+ L LC+ L + + + G CE+C+ + + R L +
Sbjct: 373 LLDEASPELVCSMLHLCSSRGLPAATVRVMPRKDGG---FCEVCKKLVGYLDRNLEKNST 429
Query: 652 FQKIFENFLINEICPHFKTK-QQQCTNDINQYYSLVVNIIVEFLNPS-LCSELGLCNSTI 825
++I + + C + ++QC + +Y +++ I+VE ++PS +C ++G C +
Sbjct: 430 KEQILA--ALEKGCSFLPDQYRKQCDQFVTEYEPVLIEILVEVMDPSFVCLKIGACPAAH 487
Query: 826 KQVI 837
K ++
Sbjct: 488 KPLL 491
Score = 63.2 bits (152), Expect = 9e-10
Identities = 47/190 (24%), Positives = 85/190 (44%), Gaps = 18/190 (9%)
Frame = +1
Query: 31 LKPIRK-----MKGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQCQ 195
++PI+K I C+ C VVK+ + I + TE EI + L + S QCQ
Sbjct: 300 VEPIKKDPAPAKADIYCEVCEFVVKEVAKLIDNNRTEEEILHALDKVCSKLPTSLAEQCQ 359
Query: 196 QIVDEYFNLILNFILAEVDVDKICKFFGLCNA------------KLSGIECELCEHVMDE 339
++VD Y IL+ +L E + +C LC++ + G CE+C+ ++
Sbjct: 360 EVVDTYGRSILSILLDEASPELVCSMLHLCSSRGLPAATVRVMPRKDGGFCEVCKKLVGY 419
Query: 340 VIKVVTSNHSEEVVSNFIKLKICPRL-SVIKNECVIIVDTYLPVIFSIARSTLNSKVICT 516
+ + + N ++E + ++ K C L + +C V Y PV+ I ++ +C
Sbjct: 420 LDRNLEKNSTKEQILAALE-KGCSFLPDQYRKQCDQFVTEYEPVLIEILVEVMDPSFVCL 478
Query: 517 ELKLCTLLSK 546
++ C K
Sbjct: 479 KIGACPAAHK 488
Score = 43.1 bits (100), Expect = 0.001
Identities = 59/268 (22%), Positives = 108/268 (40%), Gaps = 3/268 (1%)
Frame = +1
Query: 49 MKGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSD-KIQCQQIVDEYFNLI 225
+K + CD C+ V+ + D ATE EI +L+ ++ + C++IVD Y +I
Sbjct: 58 VKSLPCDICKDVITAAGNLLKDNATEQEILMYLERTCDWLPKPNMSASCKEIVDSYLPVI 117
Query: 226 LNFILAEVD-VDKICKFFGLCNAKLSGIECELCEHVMDEVIKVVTSNHSEEVVSNFIKLK 402
L+ I ++ ++C LC + L +H+ + NH +++ SN I
Sbjct: 118 LDMIKGQMSHPGEVCSALNLCES--------LQKHLAE-------LNHQKQLESNQI--- 159
Query: 403 ICPRLSVIKNECVIIVDTYLPVIFSIARSTLNSKVICTELKLCTLLSKKKISFLMNRSGN 582
P L + E V +P + + +SK KK +GN
Sbjct: 160 --PELDMA--EVVAPFMANIPFLL-YPQDGSHSKP-----------QPKK------ANGN 197
Query: 583 ETLKCEICRFAILEVR-TLLTDPGFQKIFENFLINEICPHFKTKQQQCTNDINQYYSLVV 759
C+ C + +V+ L T+ F + + E C N INQY + +
Sbjct: 198 ---VCQDCIQLVTDVQEALRTNSTFVEALVDHAKEECDRLGPGMSDMCKNYINQYSEVAI 254
Query: 760 NIIVEFLNPSLCSELGLCNSTIKQVIMR 843
+++ +C G C+ +K++ M+
Sbjct: 255 QMVMHMQPKEICVLAGFCDE-VKEMPMK 281
Score = 39.7 bits (91), Expect = 0.011
Identities = 23/100 (23%), Positives = 45/100 (45%), Gaps = 3/100 (3%)
Frame = +1
Query: 1 IHACTSVNVQLKPIRKMK---GIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIF 171
+H C+S + +R M G C+ C+ +V + +T+ +I L+ ++
Sbjct: 386 LHLCSSRGLPAATVRVMPRKDGGFCEVCKKLVGYLDRNLEKNSTKEQILAALEKGCSFLP 445
Query: 172 GSDKIQCQQIVDEYFNLILNFILAEVDVDKICKFFGLCNA 291
+ QC Q V EY +++ ++ +D +C G C A
Sbjct: 446 DQYRKQCDQFVTEYEPVLIEILVEVMDPSFVCLKIGACPA 485
>sp|Q61207|SAP_MOUSE Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)
Length = 557
Score = 53.5 bits (127), Expect = 7e-07
Identities = 58/261 (22%), Positives = 108/261 (41%), Gaps = 1/261 (0%)
Frame = +1
Query: 58 IECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILNFI 237
I C C+ V+ KF E I + ATE + L N + + +CQ++V + +L+
Sbjct: 315 ILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIF 374
Query: 238 LAEVDVDKICKFFGLCNAKLSGIECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL 417
+ EV+ +C GLC A+ +E + +VS +K P+
Sbjct: 375 IHEVNPSSLCGVIGLCAARPELVE--------------ALEQPAPAIVSALLKEPTPPKQ 420
Query: 418 SVIKNECVIIVDTYLPVIFSIARSTLNSKVICTELKLCTLLSKKKISFLMNRSGNETLKC 597
+ + ++P + C ++C KK + +L + + K
Sbjct: 421 PAQPKQSAL--PAHVP--------PQKNGGFC---EVC----KKLVLYLEHNLEKNSTKE 463
Query: 598 EICRFAILEVRTLLTDPGFQKIFENFLINEICPHFKTKQQQCTNDINQYYSLVVNIIVEF 777
EI A+ + + L DP +QK QC + + +Y L++ I+VE
Sbjct: 464 EILA-ALEKGCSFLPDP-YQK-------------------QCDDFVAEYEPLLLEILVEV 502
Query: 778 LNPS-LCSELGLCNSTIKQVI 837
++P +CS++G+C S K ++
Sbjct: 503 MDPGFVCSKIGVCPSAYKLLL 523
Score = 49.7 bits (117), Expect = 1e-05
Identities = 32/111 (28%), Positives = 56/111 (50%), Gaps = 2/111 (1%)
Frame = +1
Query: 52 KGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSD-KIQCQQIVDEYFNLIL 228
K + CD C+ VV + + D AT+ EI ++L+ +I S C+++VD Y +IL
Sbjct: 59 KSLPCDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVIL 118
Query: 229 NFILAEV-DVDKICKFFGLCNAKLSGIECELCEHVMDEVIKVVTSNHSEEV 378
+ I E+ + ++C LC + L E++ ++ K + SN EV
Sbjct: 119 DMIKGEMSNPGEVCSALNLCQS--------LQEYLAEQNQKQLESNKIPEV 161
Score = 43.5 bits (101), Expect = 8e-04
Identities = 23/84 (27%), Positives = 47/84 (55%), Gaps = 3/84 (3%)
Frame = +1
Query: 304 IECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL--SVIKNECVIIVDTYLPVIFS 477
+ C++C+ V+ E ++ N ++E + ++++ K C + S + C +VD+YLPVI
Sbjct: 61 LPCDICKTVVTEAGNLLKDNATQEEILHYLE-KTCEWIHDSSLSASCKEVVDSYLPVILD 119
Query: 478 IARSTL-NSKVICTELKLCTLLSK 546
+ + + N +C+ L LC L +
Sbjct: 120 MIKGEMSNPGEVCSALNLCQSLQE 143
Score = 38.9 bits (89), Expect = 0.019
Identities = 29/113 (25%), Positives = 53/113 (46%), Gaps = 6/113 (5%)
Frame = +1
Query: 499 SKVICTELKLCTLLSKKKI--SFLMNRSGNETLKCEICRFAILEVRTLLTDPGFQKIFEN 672
S V+C ++K K + ++ ++L C+IC+ + E LL D Q+ +
Sbjct: 29 SAVLCRDVKTAVDCGAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATQEEILH 88
Query: 673 FLINEICP--HFKTKQQQCTNDINQYYSLVVNIIV-EFLNP-SLCSELGLCNS 819
+L + C H + C ++ Y +++++I E NP +CS L LC S
Sbjct: 89 YL-EKTCEWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQS 140
>sp|P10960|SAP_RAT Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)
Length = 554
Score = 51.2 bits (121), Expect = 4e-06
Identities = 32/111 (28%), Positives = 56/111 (50%), Gaps = 2/111 (1%)
Frame = +1
Query: 52 KGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSD-KIQCQQIVDEYFNLIL 228
K + CD C+ VV + + D ATE EI ++L+ +I S C+++VD Y +IL
Sbjct: 59 KSLPCDICKTVVTEAGNLLKDNATEEEILHYLEKTCAWIHDSSLSASCKEVVDSYLPVIL 118
Query: 229 NFILAEV-DVDKICKFFGLCNAKLSGIECELCEHVMDEVIKVVTSNHSEEV 378
+ I E+ + ++C LC + L E++ ++ + + SN EV
Sbjct: 119 DMIKGEMSNPGEVCSALNLCQS--------LQEYLAEQNQRQLESNKIPEV 161
Score = 47.4 bits (111), Expect = 5e-05
Identities = 50/224 (22%), Positives = 97/224 (43%), Gaps = 44/224 (19%)
Frame = +1
Query: 304 IECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRL--SVIKNECVIIVDTYLPVIFS 477
+ C++C+ V+ E ++ N +EE + ++++ K C + S + C +VD+YLPVI
Sbjct: 61 LPCDICKTVVTEAGNLLKDNATEEEILHYLE-KTCAWIHDSSLSASCKEVVDSYLPVILD 119
Query: 478 IARSTL-NSKVICTELKLCTLLSK------------KKI----------SFLMN------ 570
+ + + N +C+ L LC L + KI F+ N
Sbjct: 120 MIKGEMSNPGEVCSALNLCQSLQEYLAEQNQRQLESNKIPEVDLARVVAPFMSNIPLLLY 179
Query: 571 ----------RSGNETLKCEICRFAILEVRTLL-TDPGF-QKIFENFLINEICPHFKTKQ 714
NE + C+ C + +++T + T+ F Q + ++ + E C
Sbjct: 180 PQDRPRSQPQPKANEDV-CQDCMKLVTDIQTAVRTNSSFVQGLVDH--VKEDCDRLGPGV 236
Query: 715 QQ-CTNDINQYYSLVVNIIVEFLNPSLCSELGLCNSTIKQVIMR 843
C N ++QY + V +++ +C +G C+ +K+V MR
Sbjct: 237 SDICKNYVDQYSEVAVQMMMHMQPKEICVMVGFCDE-VKRVPMR 279
Score = 44.3 bits (103), Expect = 4e-04
Identities = 40/210 (19%), Positives = 90/210 (42%), Gaps = 32/210 (15%)
Frame = +1
Query: 304 IECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRLSV-IKNECVIIVDTYLPVIFSI 480
I C++C+ VM ++ +++ +N +EE++ + K C L +C ++ T+ P + +
Sbjct: 312 IFCQVCQLVMRKLSELIINNATEELLIKGLS-KACSLLPAPASTKCQEVLVTFGPSLLDV 370
Query: 481 ARSTLNSKVICTELKLCTL------LSKKKISFLMNRSGNETLK---------------- 594
+N +C + LC+ ++ + +++ E
Sbjct: 371 LMHEVNPNFLCGVISLCSANPNLVGTLEQPAAAIVSALPKEPAPPKQPEEPKQSALRAHV 430
Query: 595 --------CEICRFAILEVRTLLTDPGFQKIFENFLINEICPHFKTKQQQCTNDINQYYS 750
CE+C+ ++ + L ++ L Q+QC + +Y
Sbjct: 431 PPQKNGGFCEVCKKLVIYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDEFVAEYEP 490
Query: 751 LVVNIIVEFLNPS-LCSELGLCNSTIKQVI 837
L++ I+VE ++PS +CS++G+C S K ++
Sbjct: 491 LLLEILVEVMDPSFVCSKIGVCPSAYKLLL 520
Score = 40.4 bits (93), Expect = 0.006
Identities = 22/84 (26%), Positives = 42/84 (50%)
Frame = +1
Query: 40 IRKMKGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFN 219
I+ I C C+LV++K E I + ATE + L + +CQ+++ +
Sbjct: 306 IQAQNVIFCQVCQLVMRKLSELIINNATEELLIKGLSKACSLLPAPASTKCQEVLVTFGP 365
Query: 220 LILNFILAEVDVDKICKFFGLCNA 291
+L+ ++ EV+ + +C LC+A
Sbjct: 366 SLLDVLMHEVNPNFLCGVISLCSA 389
Score = 37.7 bits (86), Expect = 0.042
Identities = 28/113 (24%), Positives = 53/113 (46%), Gaps = 6/113 (5%)
Frame = +1
Query: 499 SKVICTELKLCTLLSKKKI--SFLMNRSGNETLKCEICRFAILEVRTLLTDPGFQKIFEN 672
S V+C ++K K + ++ ++L C+IC+ + E LL D ++ +
Sbjct: 29 SAVVCRDVKTAVDCRAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATEEEILH 88
Query: 673 FLINEICP--HFKTKQQQCTNDINQYYSLVVNIIV-EFLNP-SLCSELGLCNS 819
+L + C H + C ++ Y +++++I E NP +CS L LC S
Sbjct: 89 YL-EKTCAWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQS 140
Score = 37.0 bits (84), Expect = 0.071
Identities = 18/74 (24%), Positives = 36/74 (48%)
Frame = +1
Query: 64 CDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILNFILA 243
C+ C+ +V + + +T+ EI L+ ++ + QC + V EY L+L ++
Sbjct: 439 CEVCKKLVIYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDEFVAEYEPLLLEILVE 498
Query: 244 EVDVDKICKFFGLC 285
+D +C G+C
Sbjct: 499 VMDPSFVCSKIGVC 512
>sp|P20097|SAP_CAVPO Saposin C (CO-beta-glucosidase) (Glucosylceramidase activator)
(Sphingolipid activator protein 2) (SAP-2)
Length = 81
Score = 49.3 bits (116), Expect = 1e-05
Identities = 26/78 (33%), Positives = 41/78 (52%)
Frame = +1
Query: 52 KGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLILN 231
+ + C AC VVKK E I + TE +I + L + + S CQ++VD Y + I+
Sbjct: 1 ESVTCKACEYVVKKVMELIDNNRTEEKIIHALDSVCALLPESVSEVCQEVVDTYGDSIVA 60
Query: 232 FILAEVDVDKICKFFGLC 285
+L E+ + +C GLC
Sbjct: 61 LLLQEMSPELVCSELGLC 78
Score = 45.8 bits (107), Expect = 2e-04
Identities = 22/77 (28%), Positives = 47/77 (61%), Gaps = 1/77 (1%)
Frame = +1
Query: 304 IECELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRLSVIKNE-CVIIVDTYLPVIFSI 480
+ C+ CE+V+ +V++++ +N +EE + + + +C L +E C +VDTY I ++
Sbjct: 3 VTCKACEYVVKKVMELIDNNRTEEKIIHALD-SVCALLPESVSEVCQEVVDTYGDSIVAL 61
Query: 481 ARSTLNSKVICTELKLC 531
++ +++C+EL LC
Sbjct: 62 LLQEMSPELVCSELGLC 78
Score = 35.4 bits (80), Expect = 0.21
Identities = 19/80 (23%), Positives = 43/80 (53%), Gaps = 1/80 (1%)
Frame = +1
Query: 583 ETLKCEICRFAILEVRTLLTDPGFQKIFENFLINEICPHFKTKQQQCTNDINQYYSLVVN 762
E++ C+ C + + +V L+ + ++ + L + ++ + C ++ Y +V
Sbjct: 1 ESVTCKACEYVVKKVMELIDNNRTEEKIIHALDSVCALLPESVSEVCQEVVDTYGDSIVA 60
Query: 763 IIVEFLNPSL-CSELGLCNS 819
++++ ++P L CSELGLC S
Sbjct: 61 LLLQEMSPELVCSELGLCMS 80
>sp|P42211|ASPRX_ORYSA Aspartic proteinase precursor
Length = 496
Score = 47.4 bits (111), Expect = 5e-05
Identities = 27/104 (25%), Positives = 50/104 (48%), Gaps = 18/104 (17%)
Frame = +1
Query: 187 QCQQIVDEYFNLILNFILAEVDVDKICKFFGLC-----NAKLSGIE-------------C 312
+C+++V EY +ILN ++A+ D K+C GLC + +GIE C
Sbjct: 311 ECKEVVSEYGEMILNLLIAQTDPQKVCSQVGLCMFDGKRSVSNGIESVVDKENLGSDAMC 370
Query: 313 ELCEHVMDEVIKVVTSNHSEEVVSNFIKLKICPRLSVIKNECVI 444
+CE + + + N ++E++ N+ ++C RL E +
Sbjct: 371 SVCEMAVVWIENQLRENKTKELILNYAN-QLCERLPSPNGESTV 413
Score = 36.6 bits (83), Expect = 0.093
Identities = 20/112 (17%), Positives = 50/112 (44%), Gaps = 5/112 (4%)
Frame = +1
Query: 421 VIKNECVIIVDTYLPVIFSIARSTLNSKVICTELKLCTLLSKKKI-----SFLMNRSGNE 585
+I EC +V Y +I ++ + + + +C+++ LC K+ + S + +
Sbjct: 307 IISTECKEVVSEYGEMILNLLIAQTDPQKVCSQVGLCMFDGKRSVSNGIESVVDKENLGS 366
Query: 586 TLKCEICRFAILEVRTLLTDPGFQKIFENFLINEICPHFKTKQQQCTNDINQ 741
C +C A++ + L + +++ N+ N++C + + T +Q
Sbjct: 367 DAMCSVCEMAVVWIENQLRENKTKELILNY-ANQLCERLPSPNGESTVSCHQ 417
>sp|P50405|PSPB_MOUSE Pulmonary surfactant-associated protein B precursor (SP-B)
(Pulmonary surfactant-associated proteolipid SPL(Phe))
Length = 377
Score = 44.3 bits (103), Expect = 4e-04
Identities = 25/96 (26%), Positives = 48/96 (50%), Gaps = 7/96 (7%)
Frame = +1
Query: 265 CKFFGLCNAKLSGIE-----CELCEHVMDEVIKVVTSNHSEEVVSNFIKLK--ICPRLSV 423
C+ G C ++ G C+ CE ++ + K+ + +E + F++ + I P L +
Sbjct: 48 CRALGHCLQEVWGHAGANDLCQECEDIVHLLTKMTKEDAFQEAIRKFLEQECDILP-LKL 106
Query: 424 IKNECVIIVDTYLPVIFSIARSTLNSKVICTELKLC 531
+ C ++D YLP++ +S +N K IC + LC
Sbjct: 107 LVPRCRQVLDVYLPLVIDYFQSQINPKAICNHVGLC 142
Score = 39.3 bits (90), Expect = 0.014
Identities = 19/76 (25%), Positives = 39/76 (51%), Gaps = 2/76 (2%)
Frame = +1
Query: 64 CDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKI--QCQQIVDEYFNLILNFI 237
C C +V + + A + I+ FL+ + C I + +C+Q++D Y L++++
Sbjct: 68 CQECEDIVHLLTKMTKEDAFQEAIRKFLEQE-CDILPLKLLVPRCRQVLDVYLPLVIDYF 126
Query: 238 LAEVDVDKICKFFGLC 285
++++ IC GLC
Sbjct: 127 QSQINPKAICNHVGLC 142
Score = 36.6 bits (83), Expect = 0.093
Identities = 20/94 (21%), Positives = 40/94 (42%)
Frame = +1
Query: 49 MKGIECDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKIQCQQIVDEYFNLIL 228
++ EC C+ V+ + T + + C F D+ +C+Q V+++ +L
Sbjct: 290 LQDTECHFCKSVINQAWN------TSEQAMPQAMHQACLRFWLDRQKCEQFVEQHMPQLL 343
Query: 229 NFILAEVDVDKICKFFGLCNAKLSGIECELCEHV 330
+ D C+ G+C A S ++C H+
Sbjct: 344 ALVPRSQDAHITCQALGVCEAPASPLQCFQTPHL 377
>sp|P07988|PSPB_HUMAN Pulmonary surfactant-associated protein B precursor (SP-B) (6 kDa
protein) (Pulmonary surfactant-associated proteolipid
SPL(Phe)) (18 kDa pulmonary-surfactant protein)
Length = 381
Score = 42.4 bits (98), Expect = 0.002
Identities = 19/79 (24%), Positives = 40/79 (50%), Gaps = 2/79 (2%)
Frame = +1
Query: 64 CDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKI--QCQQIVDEYFNLILNFI 237
C C +V + + + ++ FL+ + C + + QC Q++D+YF L++++
Sbjct: 69 CQECEDIVHILNKMAKEAIFQDTMRKFLEQE-CNVLPLKLLMPQCNQVLDDYFPLVIDYF 127
Query: 238 LAEVDVDKICKFFGLCNAK 294
+ D + IC GLC ++
Sbjct: 128 QNQTDSNGICMHLGLCKSR 146
Score = 33.9 bits (76), Expect = 0.60
Identities = 21/96 (21%), Positives = 46/96 (47%), Gaps = 7/96 (7%)
Frame = +1
Query: 265 CKFFGLCNAKLSGIE-----CELCEHVMDEVIKVVTSNHSEEVVSNFIKLK--ICPRLSV 423
C+ G C ++ G C+ CE ++ + K+ ++ + F++ + + P L +
Sbjct: 49 CRALGHCLQEVWGHVGADDLCQECEDIVHILNKMAKEAIFQDTMRKFLEQECNVLP-LKL 107
Query: 424 IKNECVIIVDTYLPVIFSIARSTLNSKVICTELKLC 531
+ +C ++D Y P++ ++ +S IC L LC
Sbjct: 108 LMPQCNQVLDDYFPLVIDYFQNQTDSNGICMHLGLC 143
>sp|P17129|PSPB_CANFA Pulmonary surfactant-associated protein B precursor (SP-B) (6 kDa
protein) (Pulmonary surfactant-associated proteolipid
SPL(Phe)) (Pulmonary surfactant protein 18) (SP 18)
Length = 363
Score = 41.6 bits (96), Expect = 0.003
Identities = 25/97 (25%), Positives = 50/97 (51%), Gaps = 8/97 (8%)
Frame = +1
Query: 265 CKFFGLC------NAKLSGIECELCEHVMDEVIKVVTSNHSEEVVSNFIKLK--ICPRLS 420
C+ G C NA+ + C+ C+ ++ + K+ +++V F++ + + P L
Sbjct: 42 CRALGHCLQEVWGNARADDL-CQECQDIVRILTKMTKEAIFQDMVRKFLEHECDVLP-LK 99
Query: 421 VIKNECVIIVDTYLPVIFSIARSTLNSKVICTELKLC 531
++ +C ++ TY PV+ +S +N K+IC L LC
Sbjct: 100 LLTPQCHHMLGTYFPVVVDYFQSQINPKIICKHLGLC 136
Score = 39.7 bits (91), Expect = 0.011
Identities = 22/98 (22%), Positives = 51/98 (52%), Gaps = 4/98 (4%)
Frame = +1
Query: 64 CDACRLVVKKFKEYISDPATETEIKNFLKNDFCYIFGSDKI--QCQQIVDEYFNLILNFI 237
C C+ +V+ + + + ++ FL+++ C + + QC ++ YF +++++
Sbjct: 62 CQECQDIVRILTKMTKEAIFQDMVRKFLEHE-CDVLPLKLLTPQCHHMLGTYFPVVVDYF 120
Query: 238 LAEVDVDKICKFFGLCNAKL--SGIECELCEHVMDEVI 345
++++ ICK GLC L E EL + ++D++I
Sbjct: 121 QSQINPKIICKHLGLCKPGLPEPEQESELSDPLLDKLI 158
Score = 33.9 bits (76), Expect = 0.60
Identities = 21/76 (27%), Positives = 37/76 (48%), Gaps = 3/76 (3%)
Frame = +1
Query: 595 CEICRFAILEVRTLLTDPGFQKIFENFLINE--ICPHFKTKQQQCTNDINQYYSLVVNII 768
C+ C+ + + + + FQ + FL +E + P K QC + + Y+ +VV+
Sbjct: 62 CQECQDIVRILTKMTKEAIFQDMVRKFLEHECDVLP-LKLLTPQCHHMLGTYFPVVVDYF 120
Query: 769 VEFLNPS-LCSELGLC 813
+NP +C LGLC
Sbjct: 121 QSQINPKIICKHLGLC 136
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 91,155,737
Number of Sequences: 369166
Number of extensions: 1767558
Number of successful extensions: 5211
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4946
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5194
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8598109270
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)