Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_024_L21
(676 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P68037|UB2L3_MOUSE Ubiquitin-conjugating enzyme E2 L3 (U... 192 7e-49
sp|P52487|UBC84_DROME Ubiquitin-conjugating enzyme E2-18 kD... 169 6e-42
sp|O14933|UB2L6_HUMAN Ubiquitin-conjugating enzyme E2 L6 (U... 140 3e-33
sp|Q9QZU9|UB2L6_MOUSE Ubiquitin-conjugating enzyme E2 L6 (U... 122 1e-27
sp|P70711|UB2D4_RAT Ubiquitin-conjugating enzyme E2 D4 (Ubi... 92 1e-18
sp|P61080|UB2D1_MOUSE Ubiquitin-conjugating enzyme E2 D1 (U... 91 3e-18
sp|Q9NPD8|UBE2T_HUMAN Ubiquitin-conjugating enzyme E2 T (Ub... 88 2e-17
sp|P35132|UBC9_ARATH Ubiquitin-conjugating enzyme E2-17 kDa... 87 5e-17
sp|P35129|UBC2_CAEEL Ubiquitin-conjugating enzyme E2 2 (Ubi... 86 7e-17
sp|P52490|UBC13_YEAST Ubiquitin-conjugating enzyme E2 13 (U... 86 7e-17
>sp|P68037|UB2L3_MOUSE Ubiquitin-conjugating enzyme E2 L3 (Ubiquitin-protein ligase L3)
(Ubiquitin carrier protein L3) (UbcM4)
sp|P68036|UB2L3_HUMAN Ubiquitin-conjugating enzyme E2 L3 (Ubiquitin-protein ligase L3)
(Ubiquitin carrier protein L3) (UbcH7) (E2-F1) (L-UBC)
Length = 154
Score = 192 bits (488), Expect = 7e-49
Identities = 83/140 (59%), Positives = 112/140 (80%), Gaps = 4/140 (2%)
Frame = +2
Query: 38 EVQKC----FKEIQVDDKNILVWQGLIVPENPPYNKGAFRVQIDFPVEYPFXXXXXXXXX 205
E++KC F+ IQVD+ N+L WQGLIVP+NPPY+KGAFR++I+FP EYPF
Sbjct: 13 EIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKT 72
Query: 206 XXYHPNVDEKGQICMPIITPENWKPATKIEQVIQNLVAMIHDPDADHFLRADIAEEFTKN 385
YHPN+DEKGQ+C+P+I+ ENWKPATK +QVIQ+L+A+++DP +H LRAD+AEE++K+
Sbjct: 73 KIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKD 132
Query: 386 KQTFMKNAEDHTKKYAEKRP 445
++ F KNAE+ TKKY EKRP
Sbjct: 133 RKKFCKNAEEFTKKYGEKRP 152
>sp|P52487|UBC84_DROME Ubiquitin-conjugating enzyme E2-18 kDa (Ubiquitin-protein ligase)
(Ubiquitin carrier protein)
Length = 153
Score = 169 bits (428), Expect = 6e-42
Identities = 75/149 (50%), Positives = 107/149 (71%)
Frame = +2
Query: 2 IQKELSDIQNKPEVQKCFKEIQVDDKNILVWQGLIVPENPPYNKGAFRVQIDFPVEYPFX 181
+ +ELSD+ + I+ D+++L+W GL+VPE PYNKGAFR++I+FP +YPF
Sbjct: 7 LTRELSDLVEAK--MSTLRNIESSDESLLMWTGLLVPEKAPYNKGAFRIEINFPPQYPFM 64
Query: 182 XXXXXXXXXXYHPNVDEKGQICMPIITPENWKPATKIEQVIQNLVAMIHDPDADHFLRAD 361
YHPNVDEKG++C+PII+ +NWKP T+ EQV+Q LVA++H+P+ +H LR+D
Sbjct: 65 PPKILFKTKIYHPNVDEKGEVCLPIISTDNWKPTTRTEQVLQALVAIVHNPEPEHPLRSD 124
Query: 362 IAEEFTKNKQTFMKNAEDHTKKYAEKRPQ 448
+AEEF + + FMK AE+ TKK AEKRP+
Sbjct: 125 LAEEFVREHKKFMKTAEEFTKKNAEKRPE 153
>sp|O14933|UB2L6_HUMAN Ubiquitin-conjugating enzyme E2 L6 (Ubiquitin-protein ligase L6)
(Ubiquitin carrier protein L6) (UbcH8) (Retinoic
acid-induced gene B protein) (RIG-B)
Length = 152
Score = 140 bits (353), Expect = 3e-33
Identities = 66/148 (44%), Positives = 92/148 (62%)
Frame = +2
Query: 2 IQKELSDIQNKPEVQKCFKEIQVDDKNILVWQGLIVPENPPYNKGAFRVQIDFPVEYPFX 181
+ KEL D+Q KP + + DD N+LVW L++P+ PPY+ AF ++I FP EYPF
Sbjct: 6 VVKELEDLQKKPPPY--LRNLSSDDANVLVWHALLLPDQPPYHLKAFNLRISFPPEYPFK 63
Query: 182 XXXXXXXXXXYHPNVDEKGQICMPIITPENWKPATKIEQVIQNLVAMIHDPDADHFLRAD 361
YHPNVDE GQIC+PII+ ENWKP TK QV++ L +++ P+ LR D
Sbjct: 64 PPMIKFTTKIYHPNVDENGQICLPIISSENWKPCTKTCQVLEALNVLVNRPNIREPLRMD 123
Query: 362 IAEEFTKNKQTFMKNAEDHTKKYAEKRP 445
+A+ T+N + F KNAE+ T ++ RP
Sbjct: 124 LADLLTQNPELFRKNAEEFTLRFGVDRP 151
>sp|Q9QZU9|UB2L6_MOUSE Ubiquitin-conjugating enzyme E2 L6 (Ubiquitin-protein ligase L6)
(Ubiquitin carrier protein L6) (UbcM8)
Length = 152
Score = 122 bits (305), Expect = 1e-27
Identities = 58/148 (39%), Positives = 88/148 (59%)
Frame = +2
Query: 2 IQKELSDIQNKPEVQKCFKEIQVDDKNILVWQGLIVPENPPYNKGAFRVQIDFPVEYPFX 181
+ KEL + E+ +++ DD N+LVW L++P+ PY AF+V+IDFP EYPF
Sbjct: 6 VAKELESLSK--ELPPYLRQLSSDDANVLVWHMLLLPDQLPYGLKAFQVRIDFpreYPFK 63
Query: 182 XXXXXXXXXXYHPNVDEKGQICMPIITPENWKPATKIEQVIQNLVAMIHDPDADHFLRAD 361
YHPNV E G +C+P+I+ ENWKP TK QV++ L ++ P+ + +R +
Sbjct: 64 PPTLRFTTKIYHPNVREDGLVCLPLISNENWKPYTKPYQVLEALNVLVSKPNLEEPVRLE 123
Query: 362 IAEEFTKNKQTFMKNAEDHTKKYAEKRP 445
+A+ T+N + F K AE+ T K+ RP
Sbjct: 124 LADLLTQNPEMFRKKAEEFTLKFGVDRP 151
>sp|P70711|UB2D4_RAT Ubiquitin-conjugating enzyme E2 D4 (Ubiquitin-protein ligase D4)
(Ubiquitin carrier protein D4) (Ubiquitin-conjugating
enzyme E2-17 kDa 4) (E2(17)KB 4)
Length = 147
Score = 92.0 bits (227), Expect = 1e-18
Identities = 50/145 (34%), Positives = 78/145 (53%), Gaps = 1/145 (0%)
Frame = +2
Query: 2 IQKELSDIQNKPEVQKCFKEIQVDDKNILVWQGLIV-PENPPYNKGAFRVQIDFPVEYPF 178
I KEL+D+ P Q + D + WQ I+ P + PY GAF + IDFP EYPF
Sbjct: 6 IHKELNDLAQDPPAQCSAGPVGED---MFHWQATIMGPNDSPYQGGAFFLTIDFPTEYPF 62
Query: 179 XXXXXXXXXXXYHPNVDEKGQICMPIITPENWKPATKIEQVIQNLVAMIHDPDADHFLRA 358
YHPNV+ G IC+ I+ + W PA I +V+ ++ +++ DP+ D L
Sbjct: 63 KPPKVEFTTRIYHPNVNSNGSICLDILRSQ-WSPALTISKVLLSISSLLCDPNPDDPLVP 121
Query: 359 DIAEEFTKNKQTFMKNAEDHTKKYA 433
+IA+ + ++ + + A + T+KYA
Sbjct: 122 EIAQIYKTDRDKYNRTAREWTQKYA 146
>sp|P61080|UB2D1_MOUSE Ubiquitin-conjugating enzyme E2 D1 (Ubiquitin-protein ligase D1)
(Ubiquitin carrier protein D1) (Ubiquitin-conjugating
enzyme E2-17 kDa 1) (E2(17)KB 1)
sp|P51668|UB2D1_HUMAN Ubiquitin-conjugating enzyme E2 D1 (Ubiquitin-protein ligase D1)
(Ubiquitin carrier protein D1) (UbcH5)
(Ubiquitin-conjugating enzyme E2-17 kDa 1) (E2(17)KB 1)
Length = 147
Score = 90.9 bits (224), Expect = 3e-18
Identities = 49/145 (33%), Positives = 79/145 (54%), Gaps = 1/145 (0%)
Frame = +2
Query: 2 IQKELSDIQNKPEVQKCFKEIQVDDKNILVWQGLIV-PENPPYNKGAFRVQIDFPVEYPF 178
IQKELSD+Q P C DD + WQ I+ P + Y G F + + FP +YPF
Sbjct: 6 IQKELSDLQRDPPAH-CSAGPVGDD--LFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPF 62
Query: 179 XXXXXXXXXXXYHPNVDEKGQICMPIITPENWKPATKIEQVIQNLVAMIHDPDADHFLRA 358
YHPN++ G IC+ I+ + W PA + +V+ ++ +++ DP+ D L
Sbjct: 63 KPPKIAFTTKIYHPNINSNGSICLDILRSQ-WSPALTVSKVLLSICSLLCDPNPDDPLVP 121
Query: 359 DIAEEFTKNKQTFMKNAEDHTKKYA 433
DIA+ + +K+ + ++A + T+KYA
Sbjct: 122 DIAQIYKSDKEKYNRHAREWTQKYA 146
>sp|Q9NPD8|UBE2T_HUMAN Ubiquitin-conjugating enzyme E2 T (Ubiquitin-protein ligase T)
(Ubiquitin carrier protein T)
Length = 197
Score = 88.2 bits (217), Expect = 2e-17
Identities = 44/129 (34%), Positives = 71/129 (55%), Gaps = 3/129 (2%)
Frame = +2
Query: 65 QVDDKNILVWQGLIVPENPPYNKGAFRVQIDFPVEYPFXXXXXXXXXXXYHPNVDEKGQI 244
Q+DD + G N PY KG F++++ P YPF YHPN+D G+I
Sbjct: 30 QMDDLRAQILGGA----NTPYEKGVFKLEVIIPERYPFEPPQIRFLTPIYHPNIDSAGRI 85
Query: 245 CMPI--ITPEN-WKPATKIEQVIQNLVAMIHDPDADHFLRADIAEEFTKNKQTFMKNAED 415
C+ + + P+ W+P+ I V+ ++ ++ +P+ D L ADI+ EF NK F+KNA
Sbjct: 86 CLDVLKLPPKGAWRPSLNIATVLTSIQLLMSEPNPDDPLMADISSEFKYNKPAFLKNARQ 145
Query: 416 HTKKYAEKR 442
T+K+A ++
Sbjct: 146 WTEKHARQK 154
>sp|P35132|UBC9_ARATH Ubiquitin-conjugating enzyme E2-17 kDa 9 (Ubiquitin-protein ligase
9) (Ubiquitin carrier protein 9) (UBCAT4B)
Length = 148
Score = 86.7 bits (213), Expect = 5e-17
Identities = 48/145 (33%), Positives = 73/145 (50%), Gaps = 1/145 (0%)
Frame = +2
Query: 2 IQKELSDIQNKPEVQKCFKEIQVDDKNILVWQGLIV-PENPPYNKGAFRVQIDFPVEYPF 178
I KEL D+Q P + D + WQ I+ P + PY+ G F V I FP +YPF
Sbjct: 6 ILKELKDLQKDPPTSCSAGPVAED---MFHWQATIMGPSDSPYSGGVFLVTIHFPPDYPF 62
Query: 179 XXXXXXXXXXXYHPNVDEKGQICMPIITPENWKPATKIEQVIQNLVAMIHDPDADHFLRA 358
+HPN++ G IC+ I+ E W PA I +V+ ++ +++ DP+ D L
Sbjct: 63 KPPKVAFRTKVFHPNINSNGSICLDIL-KEQWSPALTISKVLLSICSLLTDPNPDDPLVP 121
Query: 359 DIAEEFTKNKQTFMKNAEDHTKKYA 433
+IA + +K + A T+KYA
Sbjct: 122 EIAHMYKTDKNKYESTARTWTQKYA 146
>sp|P35129|UBC2_CAEEL Ubiquitin-conjugating enzyme E2 2 (Ubiquitin-protein ligase 2)
(Ubiquitin carrier protein 2)
Length = 147
Score = 86.3 bits (212), Expect = 7e-17
Identities = 49/145 (33%), Positives = 76/145 (52%), Gaps = 1/145 (0%)
Frame = +2
Query: 2 IQKELSDIQNKPEVQKCFKEIQVDDKNILVWQGLIV-PENPPYNKGAFRVQIDFPVEYPF 178
IQKEL D+ P Q C DD + WQ I+ P PY G F + I FP +YPF
Sbjct: 6 IQKELQDLGRDPPAQ-CSAGPVGDD--LFHWQATIMGPPESPYQGGVFFLTIHFPTDYPF 62
Query: 179 XXXXXXXXXXXYHPNVDEKGQICMPIITPENWKPATKIEQVIQNLVAMIHDPDADHFLRA 358
YHPN++ G IC+ I+ + W PA I +V+ ++ +++ DP+ D L
Sbjct: 63 KPPKVAFTTRIYHPNINSNGSICLDILRSQ-WSPALTISKVLLSICSLLCDPNPDDPLVP 121
Query: 359 DIAEEFTKNKQTFMKNAEDHTKKYA 433
+IA + +++ + + A + T+KYA
Sbjct: 122 EIARIYKTDRERYNQLAREWTQKYA 146
>sp|P52490|UBC13_YEAST Ubiquitin-conjugating enzyme E2 13 (Ubiquitin-protein ligase 13)
(Ubiquitin carrier protein 13)
Length = 153
Score = 86.3 bits (212), Expect = 7e-17
Identities = 43/126 (34%), Positives = 70/126 (55%), Gaps = 1/126 (0%)
Frame = +2
Query: 74 DKNILVWQGLIV-PENPPYNKGAFRVQIDFPVEYPFXXXXXXXXXXXYHPNVDEKGQICM 250
D N+ +Q I PE PY G F +++ P +YP YHPN+D G+IC+
Sbjct: 29 DDNLRYFQVTIEGPEQSPYEDGIFELELYLPDDYPMEAPKVRFLTKIYHPNIDRLGRICL 88
Query: 251 PIITPENWKPATKIEQVIQNLVAMIHDPDADHFLRADIAEEFTKNKQTFMKNAEDHTKKY 430
++ NW PA +I V+ ++ A++ P+ + L D+AE++ KN+Q A + TK Y
Sbjct: 89 DVLKT-NWSPALQIRTVLLSIQALLASPNPNDPLANDVAEDWIKNEQGAKAKAREWTKLY 147
Query: 431 AEKRPQ 448
A+K+P+
Sbjct: 148 AKKKPE 153
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 72,375,922
Number of Sequences: 369166
Number of extensions: 1414936
Number of successful extensions: 3763
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3616
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3706
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5684835195
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)