Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_024_L10
(605 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9CQ85|TIM22_MOUSE Mitochondrial import inner membrane t... 109 6e-24
sp|Q9Y584|TIM22_HUMAN Mitochondrial import inner membrane t... 106 5e-23
sp|Q12328|TIM22_YEAST Mitochondrial import inner membrane t... 62 9e-10
sp|P87146|TIM22_SCHPO Mitochondrial import inner membrane t... 61 3e-09
sp|Q9VGA2|TI17C_DROME Probable mitochondrial import inner m... 40 0.005
sp|Q9Z0V8|TI17A_MOUSE Mitochondrial import inner membrane t... 40 0.006
sp|Q99595|TI17A_HUMAN Mitochondrial import inner membrane t... 39 0.008
sp|O35092|TI17A_RAT Mitochondrial import inner membrane tra... 39 0.008
sp|Q9VN97|TI17D_DROME Probable mitochondrial import inner m... 39 0.010
sp|P87130|TIM17_SCHPO Mitochondrial import inner membrane t... 38 0.017
>sp|Q9CQ85|TIM22_MOUSE Mitochondrial import inner membrane translocase subunit Tim22
Length = 194
Score = 109 bits (272), Expect = 6e-24
Identities = 59/142 (41%), Positives = 79/142 (55%), Gaps = 2/142 (1%)
Frame = +3
Query: 156 PSYSKEEKIIRSVMDSCLFKSVLSCXXXXXXXXXXXXXTSSIDPMSTI--AGPNETVTTK 329
P+ S+E+K+I M+SC FK+VL+C T+ ID P T T K
Sbjct: 53 PAKSEEQKMIERAMESCAFKAVLACVGGFVLGGAFGIFTAGIDTNVGFDPKDPYRTPTAK 112
Query: 330 MVLKDMKTKMLSYGKNFATVGALYAGTECVIESYRGKTDIANSTLSGAFVGGLLGFRXXX 509
VLKDM + +SY KNFA VGA+++ TEC++ESYRGK+D NS +SG GG +GFR
Sbjct: 113 EVLKDMGQRGMSYAKNFAIVGAMFSCTECLVESYRGKSDWKNSVISGCITGGAIGFRAGV 172
Query: 510 XXXXXXXXXXXXXSTAIDFYMR 575
S AID+Y+R
Sbjct: 173 KAGAIGCGGFAAFSAAIDYYLR 194
>sp|Q9Y584|TIM22_HUMAN Mitochondrial import inner membrane translocase subunit Tim22
(Testis-expressed sequence 4)
Length = 194
Score = 106 bits (264), Expect = 5e-23
Identities = 58/142 (40%), Positives = 77/142 (54%), Gaps = 2/142 (1%)
Frame = +3
Query: 156 PSYSKEEKIIRSVMDSCLFKSVLSCXXXXXXXXXXXXXTSSIDPMSTI--AGPNETVTTK 329
P+ S+E+K+I M+SC FK+ L+C T+ ID P T T K
Sbjct: 53 PAKSEEQKMIEKAMESCAFKAALACVGGFVLGGAFGVFTAGIDTNVGFDPKDPYRTPTAK 112
Query: 330 MVLKDMKTKMLSYGKNFATVGALYAGTECVIESYRGKTDIANSTLSGAFVGGLLGFRXXX 509
VLKDM + +SY KNFA VGA+++ TEC+IESYRG +D NS +SG GG +GFR
Sbjct: 113 EVLKDMGQRGMSYAKNFAIVGAMFSCTECLIESYRGTSDWKNSVISGCITGGAIGFRAGL 172
Query: 510 XXXXXXXXXXXXXSTAIDFYMR 575
S AID+Y+R
Sbjct: 173 KAGAIGCGGFAAFSAAIDYYLR 194
>sp|Q12328|TIM22_YEAST Mitochondrial import inner membrane translocase subunit TIM22
Length = 207
Score = 62.4 bits (150), Expect = 9e-10
Identities = 33/83 (39%), Positives = 40/83 (48%)
Frame = +3
Query: 327 KMVLKDMKTKMLSYGKNFATVGALYAGTECVIESYRGKTDIANSTLSGAFVGGLLGFRXX 506
K+ DM K S KNF +G +YAG ECVIES R K DI N +G F G L ++
Sbjct: 112 KLQFTDMGKKSYSSAKNFGYIGMIYAGVECVIESLRAKNDIYNGVTAGFFTGAGLAYKAG 171
Query: 507 XXXXXXXXXXXXXXSTAIDFYMR 575
S AID YM+
Sbjct: 172 PQAALMGGAGFAAFSAAIDLYMK 194
>sp|P87146|TIM22_SCHPO Mitochondrial import inner membrane translocase subunit tim22
Length = 175
Score = 60.8 bits (146), Expect = 3e-09
Identities = 38/130 (29%), Positives = 58/130 (44%), Gaps = 3/130 (2%)
Frame = +3
Query: 192 VMDSCLFKSVLSCXXXXXXXXXXXXXTSSIDPMSTIAGPNETVTTKMV---LKDMKTKML 362
+ +SC+FKS ++ SS+D E + + +DM ++
Sbjct: 42 ISESCVFKSSMAGVLGFGLGGIFGMFISSLDLQHIDPKIYEKPFREQIRIQARDMGSRSF 101
Query: 363 SYGKNFATVGALYAGTECVIESYRGKTDIANSTLSGAFVGGLLGFRXXXXXXXXXXXXXX 542
S KNF +G +Y+G+EC IE++R KTDI N+ +G F GG L R
Sbjct: 102 STAKNFGLLGLIYSGSECCIEAFRAKTDIYNAIAAGVFTGGALAVRSGPKAIVLGGAGFG 161
Query: 543 XXSTAIDFYM 572
S I+ YM
Sbjct: 162 LFSYGIEKYM 171
>sp|Q9VGA2|TI17C_DROME Probable mitochondrial import inner membrane translocase subunit
Tim17 3
Length = 222
Score = 40.0 bits (92), Expect = 0.005
Identities = 20/52 (38%), Positives = 29/52 (55%)
Frame = +3
Query: 345 MKTKMLSYGKNFATVGALYAGTECVIESYRGKTDIANSTLSGAFVGGLLGFR 500
+K + S +FA GA ++ +C + YR + D NS LSGA GG+L R
Sbjct: 54 VKMRTPSIAGSFAVWGATFSTVDCALVHYRQREDAWNSILSGAATGGILAAR 105
>sp|Q9Z0V8|TI17A_MOUSE Mitochondrial import inner membrane translocase subunit Tim17 A
(Inner membrane preprotein translocase Tim17a)
Length = 171
Score = 39.7 bits (91), Expect = 0.006
Identities = 21/55 (38%), Positives = 29/55 (52%)
Frame = +3
Query: 336 LKDMKTKMLSYGKNFATVGALYAGTECVIESYRGKTDIANSTLSGAFVGGLLGFR 500
L +KT+ G +FA G L++ +C + RGK D NS SGA G +L R
Sbjct: 52 LTAIKTRAPQLGGSFAVWGGLFSTIDCSMVQIRGKEDPWNSITSGALTGAILAAR 106
>sp|Q99595|TI17A_HUMAN Mitochondrial import inner membrane translocase subunit Tim17 A
(Inner membrane preprotein translocase Tim17a)
Length = 171
Score = 39.3 bits (90), Expect = 0.008
Identities = 21/55 (38%), Positives = 29/55 (52%)
Frame = +3
Query: 336 LKDMKTKMLSYGKNFATVGALYAGTECVIESYRGKTDIANSTLSGAFVGGLLGFR 500
L +KT+ G +FA G L++ +C + RGK D NS SGA G +L R
Sbjct: 52 LTAIKTRAPQLGGSFAVWGGLFSMIDCSMVQVRGKEDPWNSITSGALTGAILAAR 106
>sp|O35092|TI17A_RAT Mitochondrial import inner membrane translocase subunit Tim17 A
(Inner membrane preprotein translocase Tim17a)
Length = 171
Score = 39.3 bits (90), Expect = 0.008
Identities = 21/55 (38%), Positives = 29/55 (52%)
Frame = +3
Query: 336 LKDMKTKMLSYGKNFATVGALYAGTECVIESYRGKTDIANSTLSGAFVGGLLGFR 500
L +KT+ G +FA G L++ +C + RGK D NS SGA G +L R
Sbjct: 52 LTAIKTRAPQLGGSFAVWGGLFSTIDCGMVQIRGKEDPWNSITSGALTGAILAAR 106
>sp|Q9VN97|TI17D_DROME Probable mitochondrial import inner membrane translocase subunit
Tim17 4
Length = 224
Score = 38.9 bits (89), Expect = 0.010
Identities = 18/55 (32%), Positives = 33/55 (60%)
Frame = +3
Query: 336 LKDMKTKMLSYGKNFATVGALYAGTECVIESYRGKTDIANSTLSGAFVGGLLGFR 500
++ ++ + + +FA GA ++ +CV+ SYR + D N+ +SGA GG+L R
Sbjct: 51 IESVRLRTPAIAGSFAIWGATFSTVDCVMVSYRQREDSWNAIVSGAATGGILAAR 105
>sp|P87130|TIM17_SCHPO Mitochondrial import inner membrane translocase subunit tim17
(Mitochondrial protein import protein 2)
Length = 164
Score = 38.1 bits (87), Expect = 0.017
Identities = 18/51 (35%), Positives = 27/51 (52%)
Frame = +3
Query: 348 KTKMLSYGKNFATVGALYAGTECVIESYRGKTDIANSTLSGAFVGGLLGFR 500
KT+ G NF G L++ +C ++ R K D N+ ++G F GG L R
Sbjct: 56 KTRAPVLGGNFGVWGGLFSTFDCAVKGVRRKEDPWNAIIAGFFTGGALAVR 106
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 60,375,301
Number of Sequences: 369166
Number of extensions: 1107794
Number of successful extensions: 2043
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2010
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2041
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4699949280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)