Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_024_K20
(827 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P32802|EMP70_YEAST Endosomal P24A protein precursor (70 ... 33 1.3
sp|P87169|MAD1_SCHPO Spindle assembly checkpoint component ... 32 1.6
sp|Q90631|KTN1_CHICK Kinectin 30 6.2
sp|Q9PHM5|RECN_CAMJE DNA repair protein recN (Recombination... 30 6.2
sp|O51597|SECF_BORBU Protein-export membrane protein secF 30 6.2
>sp|P32802|EMP70_YEAST Endosomal P24A protein precursor (70 kDa endomembrane protein)
(Pheromone alpha-factor transporter) (Acidic 24 kDa late
endocytic intermediate component)
Length = 667
Score = 32.7 bits (73), Expect = 1.3
Identities = 15/42 (35%), Positives = 28/42 (66%), Gaps = 4/42 (9%)
Frame = +1
Query: 679 SVILILLTMNFVLNFIKS----PLYSIFHLVFWYFLYPLCLS 792
+++LI++ +NF L F+ S P ++F +VF +FL+ + LS
Sbjct: 448 AILLIIIALNFFLMFVHSSGVIPASTLFFMVFLWFLFSIPLS 489
>sp|P87169|MAD1_SCHPO Spindle assembly checkpoint component mad1 (Mitotic arrest
deficient protein 1)
Length = 689
Score = 32.3 bits (72), Expect = 1.6
Identities = 21/77 (27%), Positives = 33/77 (42%), Gaps = 2/77 (2%)
Frame = +2
Query: 419 NIEINRQNHMSRESS--RFESNYDWXXXXXXXXXXXXXXXSQWKSLVDNSIDLIDQMRNV 592
N+EI ++ ES RFE D + WKSL+ N + + + N
Sbjct: 266 NVEILKEEKNDLESKLYRFEEYRDKVATLELENEKIQTELNSWKSLITNELPTPEAVSNK 325
Query: 593 LVTIKSNLENLDEEIKS 643
LV +++ NL E + S
Sbjct: 326 LVFLQNTNANLGERVSS 342
>sp|Q90631|KTN1_CHICK Kinectin
Length = 1364
Score = 30.4 bits (67), Expect = 6.2
Identities = 14/36 (38%), Positives = 23/36 (63%)
Frame = +2
Query: 533 SQWKSLVDNSIDLIDQMRNVLVTIKSNLENLDEEIK 640
S+WK V+ S + QMR+ + +++ +E L EEIK
Sbjct: 1168 SKWKIKVEESQKELKQMRSSVASLEHEVERLKEEIK 1203
>sp|Q9PHM5|RECN_CAMJE DNA repair protein recN (Recombination protein N)
Length = 507
Score = 30.4 bits (67), Expect = 6.2
Identities = 16/57 (28%), Positives = 30/57 (52%)
Frame = -1
Query: 818 MRDYDDVSFDKHKGYKKYQNTK*KIEYNGDLIKLSTKFMVNNIKMTEKVFLYYQNEM 648
+ Y+++SF+K + KK+Q K K+E ++ + K N+K EK Y ++
Sbjct: 299 LEHYENLSFEKKELEKKFQELKQKLEEKAQILTQTRK---KNLKKLEKCLNNYLKDL 352
>sp|O51597|SECF_BORBU Protein-export membrane protein secF
Length = 299
Score = 30.4 bits (67), Expect = 6.2
Identities = 13/31 (41%), Positives = 22/31 (70%)
Frame = +1
Query: 673 TFSVILILLTMNFVLNFIKSPLYSIFHLVFW 765
TF +ILI +T+ F L++ + + SIFH +F+
Sbjct: 148 TFILILIYITLRFKLSYAIASILSIFHDIFF 178
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 83,801,246
Number of Sequences: 369166
Number of extensions: 1533893
Number of successful extensions: 4037
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 3920
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4035
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8004331590
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)