Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_024_I21
(752 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 117 4e-26
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 114 2e-25
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 112 1e-24
sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinas... 108 1e-23
sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinas... 108 2e-23
sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinas... 107 3e-23
sp|P41892|CDC7_SCHPO Cell division control protein 7 104 3e-22
sp|Q10407|MKH1_SCHPO MAP kinase kinase kinase mkh1 101 2e-21
sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinas... 101 2e-21
sp|Q01389|BCK1_YEAST Serine/threonine-protein kinase BCK1/S... 100 5e-21
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 117 bits (292), Expect = 4e-26
Identities = 71/191 (37%), Positives = 105/191 (54%), Gaps = 1/191 (0%)
Frame = +3
Query: 12 HPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHS 191
HP I++ G R + I ++++ GS+ S L K G+ EP II ++KQ+L L++LH+
Sbjct: 130 HPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYTKQLLLGLEYLHN 189
Query: 192 QNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVI 371
I+HRD+KG IL+DN I+L F ++K + ++ + A+S GT W APEVI
Sbjct: 190 NGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATV----NGAKSMKGTPYWMAPEVI 245
Query: 372 NGEGYGSSADIWSFGAVMVELITGKAPLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALK 548
G+ SADIWS G ++E+ TGK P F A+ +G + K P S K
Sbjct: 246 LQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIG-RTKAHPPIPEDLSPEAK 304
Query: 549 SFVDPCLDPEP 581
F+ CL EP
Sbjct: 305 DFLMKCLHKEP 315
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 114 bits (286), Expect = 2e-25
Identities = 71/191 (37%), Positives = 103/191 (53%), Gaps = 1/191 (0%)
Frame = +3
Query: 12 HPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHS 191
HP I++ G R + I L+++ GS+ S L K GA E + ++ Q+L L++LH+
Sbjct: 130 HPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRTYTNQLLLGLEYLHN 189
Query: 192 QNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVI 371
I+HRD+KG IL+DN IKL F ++K + ++ + A+S GT W APEVI
Sbjct: 190 HAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATIS----GAKSMKGTPYWMAPEVI 245
Query: 372 NGEGYGSSADIWSFGAVMVELITGKAPLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALK 548
G+ SADIWS G ++E++TGKAP AI+ +GT K P S
Sbjct: 246 LQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKS-HPPIPDNISSDAN 304
Query: 549 SFVDPCLDPEP 581
F+ CL EP
Sbjct: 305 DFLLKCLQQEP 315
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 112 bits (280), Expect = 1e-24
Identities = 72/191 (37%), Positives = 103/191 (53%), Gaps = 1/191 (0%)
Frame = +3
Query: 12 HPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHS 191
HP I++ G R I L+++ GS+ S L K G E + +++Q+L L++LH+
Sbjct: 131 HPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYTRQLLLGLEYLHN 190
Query: 192 QNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVI 371
I+HRD+KG IL+DN IKL F ++K + ++ T A+S GT W APEVI
Sbjct: 191 HAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMT----GAKSMKGTPYWMAPEVI 246
Query: 372 NGEGYGSSADIWSFGAVMVELITGKAPLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALK 548
G+ SADIWS G ++E++TGKAP AI+ +GT K SDA K
Sbjct: 247 LQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPDTLSSDA-K 305
Query: 549 SFVDPCLDPEP 581
F+ CL P
Sbjct: 306 DFLLKCLQEVP 316
>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 108 bits (271), Expect = 1e-23
Identities = 64/166 (38%), Positives = 94/166 (56%), Gaps = 3/166 (1%)
Frame = +3
Query: 12 HPRILKCNGFFR--SVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFL 185
H RI++ G R + K I ++YM GS++ L GA E ++++QILE + +L
Sbjct: 421 HERIVQYYGCLRDRAEKTLTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYL 480
Query: 186 HSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPD-ASSETFRNHVAESNHGTANWTAP 362
HS I+HRD+KG IL D+ ++KL F ++K S T V GT W +P
Sbjct: 481 HSNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGMRSVT----GTPYWMSP 536
Query: 363 EVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQ 500
EVI+GEGYG AD+WS G +VE++T K P + AI+++ TQ
Sbjct: 537 EVISGEGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQ 582
>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 108 bits (269), Expect = 2e-23
Identities = 62/165 (37%), Positives = 93/165 (56%), Gaps = 2/165 (1%)
Frame = +3
Query: 12 HPRILKCNGFFR--SVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFL 185
H RI++ G R + K I ++YM GS++ L GA E ++++QILE + +L
Sbjct: 421 HERIVQYYGCLRDRAEKILTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYL 480
Query: 186 HSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPE 365
HS I+HRD+KG IL D+ ++KL F ++K + S GT W +PE
Sbjct: 481 HSNMIVHRDIKGANILRDSAGNVKLGDFGASKRL---QTICMSGTGIRSVTGTPYWMSPE 537
Query: 366 VINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQ 500
VI+GEGYG AD+WS G +VE++T K P + AI+++ TQ
Sbjct: 538 VISGEGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQ 582
>sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 618
Score = 107 bits (267), Expect = 3e-23
Identities = 62/165 (37%), Positives = 92/165 (55%), Gaps = 2/165 (1%)
Frame = +3
Query: 12 HPRILKCNGFFRSV--KASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFL 185
H RI++ G R K I ++YM GS++ L GA E ++++QILE + +L
Sbjct: 414 HERIVQYYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGALTENGTRKYTRQILEGVHYL 473
Query: 186 HSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPE 365
HS ILHRD+KG IL D+ ++KL F ++K + +S GT W +PE
Sbjct: 474 HSNMILHRDIKGANILRDSTGNVKLGDFGASKRL---QTICLSGTGMKSVTGTPYWMSPE 530
Query: 366 VINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQ 500
VI+G+GYG ADIWS +VE++T K P + AI+++ TQ
Sbjct: 531 VISGQGYGRKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIATQ 575
>sp|P41892|CDC7_SCHPO Cell division control protein 7
Length = 1062
Score = 104 bits (259), Expect = 3e-22
Identities = 66/191 (34%), Positives = 97/191 (50%)
Frame = +3
Query: 9 DHPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLH 188
DHP I+K G +++ + I L+Y NGSLRS G E + ++ Q+L+ L +LH
Sbjct: 64 DHPNIVKYRGSYQTNDSLCIILEYCENGSLRSICKNFGKIPENLVALYTFQVLQGLLYLH 123
Query: 189 SQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEV 368
+Q ++HRD+KG IL + IKL F A +H S G+ W APEV
Sbjct: 124 NQGVIHRDIKGANILTTKDGTIKLADFGVA-----TKINALEDH---SVVGSPYWMAPEV 175
Query: 369 INGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLKCSDALK 548
I G +++DIWS G ++EL+ G P + L A++R+ K P S A K
Sbjct: 176 IELVGATTASDIWSVGCTVIELLDGNPPYYDLDPTSALFRM--VKDEHPPLPSNISSAAK 233
Query: 549 SFVDPCLDPEP 581
SF+ C +P
Sbjct: 234 SFLMQCFQKDP 244
>sp|Q10407|MKH1_SCHPO MAP kinase kinase kinase mkh1
Length = 1116
Score = 101 bits (252), Expect = 2e-21
Identities = 58/168 (34%), Positives = 94/168 (55%), Gaps = 2/168 (1%)
Frame = +3
Query: 9 DHPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLH 188
DH I++ GF ++ I L+Y++ GS+ L G +E + S+Q+L L +LH
Sbjct: 888 DHLNIVQYLGFEKTETDISIFLEYVSGGSIGRCLRNYGPFEEQLVRFVSRQVLYGLSYLH 947
Query: 189 SQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEV 368
S+ I+HRDLK +L+D + K+ F +K S + N S G+ W APEV
Sbjct: 948 SKGIIHRDLKADNLLIDFDGVCKISDFGISK----HSDNVYDNDANLSMQGSIFWMAPEV 1003
Query: 369 ING--EGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKK 506
I+ +GY + D+WS G V++E++ G+ P + QA+++LGT+KK
Sbjct: 1004 IHNDHQGYSAKVDVWSLGCVVLEMLAGRRPWSTDEAIQAMFKLGTEKK 1051
>sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 619
Score = 101 bits (252), Expect = 2e-21
Identities = 61/165 (36%), Positives = 90/165 (54%), Gaps = 2/165 (1%)
Frame = +3
Query: 12 HPRILKCNGFFRSV--KASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFL 185
H RI++ G R K I ++ GS++ L GA E ++++QILE + +L
Sbjct: 415 HERIVQYYGCLRDPQEKTLSIFMELSPGGSIKDQLKAYGALTENVTRKYTRQILEGVHYL 474
Query: 186 HSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPE 365
HS I+HRD+KG IL D+ +IKL F ++K + +S GT W +PE
Sbjct: 475 HSNMIVHRDIKGANILRDSTGNIKLGDFGASKRL---QTICLSGTGMKSVTGTPYWMSPE 531
Query: 366 VINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQ 500
VI+GEGYG ADIWS +VE++T K P + AI+++ TQ
Sbjct: 532 VISGEGYGRKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIATQ 576
>sp|Q01389|BCK1_YEAST Serine/threonine-protein kinase BCK1/SLK1/SSP31
Length = 1478
Score = 100 bits (248), Expect = 5e-21
Identities = 63/196 (32%), Positives = 100/196 (51%), Gaps = 5/196 (2%)
Frame = +3
Query: 9 DHPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLH 188
DH I++ GF + L+Y+A GS+ S + G EP I + Q+L+ L +LH
Sbjct: 1236 DHLNIVQYLGFENKNNIYSLFLEYVAGGSVGSLIRMYGRFDEPLIKHLTTQVLKGLAYLH 1295
Query: 189 SQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEV 368
S+ ILHRD+K +LLD + K+ F ++ D S + + GT W APE+
Sbjct: 1296 SKGILHRDMKADNLLLDQDGICKISDFGISRKSKDIYSNS-----DMTMRGTVFWMAPEM 1350
Query: 369 IN-GEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKI----EFAYPLKC 533
++ +GY + DIWS G +++E+ GK P L A++++G K E PL
Sbjct: 1351 VDTKQGYSAKVDIWSLGCIVLEMFAGKRPWSNLEVVAAMFKIGKSKSAPPIPEDTLPL-I 1409
Query: 534 SDALKSFVDPCLDPEP 581
S ++F+D C + P
Sbjct: 1410 SQIGRNFLDACFEINP 1425
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 93,501,601
Number of Sequences: 369166
Number of extensions: 1951507
Number of successful extensions: 7814
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6050
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6459
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6873311200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)