Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_024_I19
(742 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q24562|U2AF2_DROME Splicing factor U2AF 50 kDa subunit (... 245 1e-64
sp|P26368|U2AF2_HUMAN Splicing factor U2AF 65 kDa subunit (... 241 1e-63
sp|P26369|U2AF2_MOUSE Splicing factor U2AF 65 kDa subunit (... 241 1e-63
sp|P90727|U2AF2_CAEBR Splicing factor U2AF 65 kDa subunit (... 214 3e-55
sp|P90978|U2AF2_CAEEL Splicing factor U2AF 65 kDa subunit (... 213 4e-55
sp|P36629|U2AF2_SCHPO Splicing factor U2AF 59 kDa subunit (... 132 1e-30
sp|Q8TAS1|UHMK1_HUMAN Serine/threonine-protein kinase Kist ... 89 2e-17
sp|P97343|UHMK1_MOUSE Serine/threonine-protein kinase Kist ... 87 4e-17
sp|Q63285|UHMK1_RAT Serine/threonine-protein kinase Kist (K... 87 4e-17
sp|O43040|CTF1_SCHPO Cleavage and termination factor 1 (Tra... 65 2e-10
>sp|Q24562|U2AF2_DROME Splicing factor U2AF 50 kDa subunit (U2 auxiliary factor 50 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
Length = 416
Score = 245 bits (625), Expect = 1e-64
Identities = 128/242 (52%), Positives = 170/242 (70%), Gaps = 10/242 (4%)
Frame = +3
Query: 6 DYVPLPQAFD------GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLR 167
DY P+P D +VS+ + + +V D+PHK+F+GGLP YL +DQVKELL +FG LR
Sbjct: 175 DYQPMPGITDTPAIKPAVVSSGVISTVVPDSPHKIFIGGLPNYLNDDQVKELLLSFGKLR 234
Query: 168 GFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNSTGIP 347
FNLVKD+ TGLSKGYAFCEY D S+TD++ GL GMQLG+K+++VQRA +GAKN+
Sbjct: 235 AFNLVKDAATGLSKGYAFCEYVDLSITDQSIAGLNGMQLGDKKLIVQRASVGAKNAQNAA 294
Query: 348 TGPLMLTQPMMMDPLKMVNV----EETEVLCLHNMVMIDDLNDDEEYEEIMEDVKEECSK 515
TQ +M+ + NV TEVLCL NMV D+L D+EEYE+I+ED+KEEC+K
Sbjct: 295 N----TTQSVMLQVPGLSNVVTSGPPTEVLCLLNMVTPDELRDEEEYEDILEDIKEECTK 350
Query: 516 FGNVLSVQIPRPTGTDDNVSGVGKIYVEFQNKSETVKAMKALSGRTFSGKVVVTSFYDLE 695
+G V SV+IPRP V G GK++VEF + + KA +AL+GR FS +VVVTS++D +
Sbjct: 351 YGVVRSVEIPRPI-EGVEVPGCGKVFVEFNSVLDCQKAQQALTGRKFSDRVVVTSYFDPD 409
Query: 696 KY 701
KY
Sbjct: 410 KY 411
>sp|P26368|U2AF2_HUMAN Splicing factor U2AF 65 kDa subunit (U2 auxiliary factor 65 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
(hU2AF(65))
Length = 475
Score = 241 bits (615), Expect = 1e-63
Identities = 127/240 (52%), Positives = 167/240 (69%), Gaps = 8/240 (3%)
Frame = +3
Query: 6 DYVPLPQAFDG--IVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNL 179
DY PLP + + + + +V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNL
Sbjct: 231 DYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNL 290
Query: 180 VKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNST------G 341
VKDS TGLSKGYAFCEY D +VTD+A GL GMQLG+K+++VQRA +GAKN+T
Sbjct: 291 VKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLVSPPST 350
Query: 342 IPTGPLMLTQPMMMDPLKMVNVEETEVLCLHNMVMIDDLNDDEEYEEIMEDVKEECSKFG 521
I P+ L P +M + TEVLCL NMV+ ++L DDEEYEEI+EDV++ECSK+G
Sbjct: 351 INQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYG 410
Query: 522 NVLSVQIPRPTGTDDNVSGVGKIYVEFQNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY 701
V S++IPRP + V G GKI+VEF + + KAM+ L+GR F+ +VVVT + D + Y
Sbjct: 411 LVKSIEIPRPVDGVE-VPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSY 469
>sp|P26369|U2AF2_MOUSE Splicing factor U2AF 65 kDa subunit (U2 auxiliary factor 65 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
Length = 475
Score = 241 bits (615), Expect = 1e-63
Identities = 127/240 (52%), Positives = 167/240 (69%), Gaps = 8/240 (3%)
Frame = +3
Query: 6 DYVPLPQAFDG--IVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNL 179
DY PLP + + + + +V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNL
Sbjct: 231 DYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNL 290
Query: 180 VKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNST------G 341
VKDS TGLSKGYAFCEY D +VTD+A GL GMQLG+K+++VQRA +GAKN+T
Sbjct: 291 VKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLVSLPST 350
Query: 342 IPTGPLMLTQPMMMDPLKMVNVEETEVLCLHNMVMIDDLNDDEEYEEIMEDVKEECSKFG 521
I P+ L P +M + TEVLCL NMV+ ++L DDEEYEEI+EDV++ECSK+G
Sbjct: 351 INQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYG 410
Query: 522 NVLSVQIPRPTGTDDNVSGVGKIYVEFQNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY 701
V S++IPRP + V G GKI+VEF + + KAM+ L+GR F+ +VVVT + D + Y
Sbjct: 411 LVKSIEIPRPVDGVE-VPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSY 469
>sp|P90727|U2AF2_CAEBR Splicing factor U2AF 65 kDa subunit (U2 auxiliary factor 65 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
(U2AF65)
Length = 488
Score = 214 bits (544), Expect = 3e-55
Identities = 119/239 (49%), Positives = 158/239 (66%), Gaps = 1/239 (0%)
Frame = +3
Query: 3 RDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLV 182
RDY P FD + + + IV D+ +K+F+GGLP YL EDQVKELL +FGPL+ F+L
Sbjct: 256 RDYQPSQNTFD-MNARMPVSSIVVDSANKIFIGGLPNYLTEDQVKELLCSFGPLKAFSLN 314
Query: 183 KDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLG-AKNSTGIPTGPL 359
DS G SKGYAF EY D ++TD+A GL GMQLG+K++VVQ AC +++T +P
Sbjct: 315 VDSQ-GNSKGYAFAEYLDPTLTDQAIAGLNGMQLGDKQLVVQLACANQTRHNTHLPNSAS 373
Query: 360 MLTQPMMMDPLKMVNVEETEVLCLHNMVMIDDLNDDEEYEEIMEDVKEECSKFGNVLSVQ 539
+ L TE+LCL NMV D+L DE+YEEI+EDV+EECSK+G V S++
Sbjct: 374 AIAGI----DLSQGAGRATEILCLMNMVTEDELRSDEDYEEILEDVREECSKYGIVRSLE 429
Query: 540 IPRPTGTDDNVSGVGKIYVEFQNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYKNGFF 716
IPRP D V GVGK++VEF S+ +A AL+GR F+ + VVTS+YD++KY N F
Sbjct: 430 IPRPY-DDHPVPGVGKVFVEFATTSDCQRAQAALTGRKFANRTVVTSYYDVDKYHNRQF 487
>sp|P90978|U2AF2_CAEEL Splicing factor U2AF 65 kDa subunit (U2 auxiliary factor 65 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
(U2AF65)
Length = 496
Score = 213 bits (542), Expect = 4e-55
Identities = 119/239 (49%), Positives = 159/239 (66%), Gaps = 1/239 (0%)
Frame = +3
Query: 3 RDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLV 182
RDY P FD + S + IV D+ +K+F+GGLP YL EDQVKELL +FGPL+ F+L
Sbjct: 265 RDYQPSQNTFD-MNSRMPVSTIVVDSANKIFIGGLPNYLTEDQVKELLCSFGPLKAFSLN 323
Query: 183 KDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGA-KNSTGIPTGPL 359
DS G SKGYAF EY D ++TD+A GL GMQLG+K++VVQ AC +++T +P
Sbjct: 324 VDSQ-GNSKGYAFAEYLDPTLTDQAIAGLNGMQLGDKQLVVQLACANQQRHNTNLPNSAS 382
Query: 360 MLTQPMMMDPLKMVNVEETEVLCLHNMVMIDDLNDDEEYEEIMEDVKEECSKFGNVLSVQ 539
+ L TE+LCL NMV D+L D+EYEEI+EDV++ECSK+G V S++
Sbjct: 383 AIAGI----DLSQGAGRATEILCLMNMVTEDELKADDEYEEILEDVRDECSKYGIVRSLE 438
Query: 540 IPRPTGTDDNVSGVGKIYVEFQNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYKNGFF 716
IPRP D V GVGK++VEF + S+ +A AL+GR F+ + VVTS+YD++KY N F
Sbjct: 439 IPRPY-EDHPVPGVGKVFVEFASTSDCQRAQAALTGRKFANRTVVTSYYDVDKYHNRQF 496
>sp|P36629|U2AF2_SCHPO Splicing factor U2AF 59 kDa subunit (U2 auxiliary factor 59 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
(U2AF59)
Length = 517
Score = 132 bits (332), Expect = 1e-30
Identities = 71/212 (33%), Positives = 117/212 (55%)
Frame = +3
Query: 69 VQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVT 248
V D+ K+++ LP LGEDQV ELL+ FG L F L+K+ G SKG+ FCE+ + S
Sbjct: 305 VLDSKDKIYISNLPLNLGEDQVVELLKPFGDLLSFQLIKNIADGSSKGFCFCEFKNPSDA 364
Query: 249 DKACMGLTGMQLGEKRIVVQRACLGAKNSTGIPTGPLMLTQPMMMDPLKMVNVEETEVLC 428
+ A GL G ++ Q AC+G + + + + + + + T VL
Sbjct: 365 EVAISGLDGKDTYGNKLHAQFACVGLNQAMIDKSNGMAILTELAKASSQSI---PTRVLQ 421
Query: 429 LHNMVMIDDLNDDEEYEEIMEDVKEECSKFGNVLSVQIPRPTGTDDNVSGVGKIYVEFQN 608
LHN++ D++ D +EYE+I E VK + S +G ++ ++IPR GT ++ G GK++V + +
Sbjct: 422 LHNLITGDEIMDVQEYEDIYESVKTQFSNYGPLIDIKIPRSIGTRNSGLGTGKVFVRYSD 481
Query: 609 KSETVKAMKALSGRTFSGKVVVTSFYDLEKYK 704
AM+ + G F+ + +V +FY + YK
Sbjct: 482 IRSAEVAMEEMKGCKFNDRTIVIAFYGEDCYK 513
>sp|Q8TAS1|UHMK1_HUMAN Serine/threonine-protein kinase Kist (Kinase interacting with
stathmin) (U2AF homology motif kinase 1)
Length = 419
Score = 88.6 bits (218), Expect = 2e-17
Identities = 44/100 (44%), Positives = 65/100 (65%)
Frame = +3
Query: 414 TEVLCLHNMVMIDDLNDDEEYEEIMEDVKEECSKFGNVLSVQIPRPTGTDDNVSGVGKIY 593
T VL L N++ D L ++EEYE+++EDVKEEC K+G V+S+ +P+ G G+++
Sbjct: 320 TPVLRLLNVLDDDYLENEEEYEDVVEDVKEECQKYGPVVSLLVPKEN------PGRGQVF 373
Query: 594 VEFQNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYKNGF 713
VE+ N ++ A K L+GR F GK VV +FY L YK G+
Sbjct: 374 VEYANAGDSKAAQKLLTGRMFDGKFVVATFYPLSAYKRGY 413
>sp|P97343|UHMK1_MOUSE Serine/threonine-protein kinase Kist (Kinase interacting with
stathmin) (U2AF homology motif kinase 1)
Length = 419
Score = 87.4 bits (215), Expect = 4e-17
Identities = 43/100 (43%), Positives = 65/100 (65%)
Frame = +3
Query: 414 TEVLCLHNMVMIDDLNDDEEYEEIMEDVKEECSKFGNVLSVQIPRPTGTDDNVSGVGKIY 593
T VL L N++ D L +++EYE+++EDVKEEC K+G V+S+ +P+ G G+++
Sbjct: 320 TPVLRLLNVLDDDYLENEDEYEDVVEDVKEECQKYGPVVSLLVPKEN------PGRGQVF 373
Query: 594 VEFQNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYKNGF 713
VE+ N ++ A K L+GR F GK VV +FY L YK G+
Sbjct: 374 VEYANAGDSKAAQKLLTGRMFDGKFVVATFYPLSAYKRGY 413
>sp|Q63285|UHMK1_RAT Serine/threonine-protein kinase Kist (Kinase interacting with
stathmin) (U2AF homology motif kinase 1) (PAM
COOH-terminal interactor protein 2) (P-CIP2)
Length = 419
Score = 87.4 bits (215), Expect = 4e-17
Identities = 43/100 (43%), Positives = 65/100 (65%)
Frame = +3
Query: 414 TEVLCLHNMVMIDDLNDDEEYEEIMEDVKEECSKFGNVLSVQIPRPTGTDDNVSGVGKIY 593
T VL L N++ D L +++EYE+++EDVKEEC K+G V+S+ +P+ G G+++
Sbjct: 320 TPVLRLLNVLDDDYLENEDEYEDVVEDVKEECQKYGPVVSLLVPKEN------PGRGQVF 373
Query: 594 VEFQNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYKNGF 713
VE+ N ++ A K L+GR F GK VV +FY L YK G+
Sbjct: 374 VEYANAGDSKAAQKLLTGRMFDGKFVVATFYPLSAYKRGY 413
>sp|O43040|CTF1_SCHPO Cleavage and termination factor 1 (Transcription termination factor
ctf1)
Length = 363
Score = 64.7 bits (156), Expect = 2e-10
Identities = 35/86 (40%), Positives = 47/86 (54%)
Frame = +3
Query: 51 SMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEY 230
SMTAG V +FVG +P + E Q+ E+ GP++ F LV D TG KGY FCE+
Sbjct: 2 SMTAGNV------VFVGNIPYDVSEQQMTEIFNQVGPVKTFKLVLDPETGSGKGYGFCEF 55
Query: 231 ADHSVTDKACMGLTGMQLGEKRIVVQ 308
D T A L +LG ++I V+
Sbjct: 56 FDSETTAMAVRKLNNSELGPRKIRVE 81
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 90,803,863
Number of Sequences: 369166
Number of extensions: 1903845
Number of successful extensions: 5829
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5408
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5785
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6679696800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)