Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_024_H10
(782 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O70352|CD82_RAT CD82 antigen (Metastasis suppressor homo... 69 1e-11
sp|P40237|CD82_MOUSE CD82 antigen (Inducible membrane prote... 66 9e-11
sp|O60635|TSN1_HUMAN Tetraspanin-1 (Tspan-1) (Tetraspan NET... 60 9e-09
sp|Q96SJ8|TSN18_HUMAN Tetraspanin-18 (Tspan-18) 58 3e-08
sp|Q58CY8|TSN18_BOVIN Tetraspanin-18 (Tspan-18) 58 3e-08
sp|Q5RC27|TSN1_PONPY Tetraspanin-1 (Tspan-1) 56 1e-07
sp|Q80WR1|TSN18_MOUSE Tetraspanin-18 (Tspan-18) 55 2e-07
sp|O14817|TSN4_HUMAN Tetraspanin-4 (Tspan-4) (Transmembrane... 55 3e-07
sp|P27701|CD82_HUMAN CD82 antigen (Inducible membrane prote... 55 3e-07
sp|Q9DCK3|TSN4_MOUSE Tetraspanin-4 (Tspan-4) (Transmembrane... 55 3e-07
>sp|O70352|CD82_RAT CD82 antigen (Metastasis suppressor homolog)
Length = 266
Score = 69.3 bits (168), Expect = 1e-11
Identities = 50/190 (26%), Positives = 84/190 (44%), Gaps = 2/190 (1%)
Frame = +2
Query: 2 FNILFFLIGAVILGVGIWVKVDKSNLFEVIRNIKLDDTTASFNNTNSTILDVVAIVLIIX 181
FN+LFF++GAVILG G+W+ DKS+ V++ T+S+ L V A V I
Sbjct: 16 FNLLFFILGAVILGFGVWILADKSSFISVLQ-------------TSSSSLQVGAYVFIGV 62
Query: 182 XXXXXXXXXXXXXXAIKENKCLLITYSILXXXXXXXXXXXXXXXXXEKNKFQKNLGKSLT 361
A+ E +CLL Y + NK ++ +G ++
Sbjct: 63 GAITMLMGFLGCIGAVNEVRCLLGLYFVFLLLILIAQVTVEVLFYFNANKLKQEMGNTVM 122
Query: 362 NVLEKDFNGILNTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNW--KSTSSSGTKQIY 535
++++ N +N +++ + + + ++KCCG + S NW + TK Y
Sbjct: 123 DIIQ---NYSVNASSSR-EEAWDYVQAQVKCCG------WVSPSNWTRNPVLKNSTKTTY 172
Query: 536 PVGCCKLKEK 565
P C K KE+
Sbjct: 173 PCSCEKTKEE 182
>sp|P40237|CD82_MOUSE CD82 antigen (Inducible membrane protein R2) (C33 antigen) (IA4)
Length = 266
Score = 66.2 bits (160), Expect = 9e-11
Identities = 50/191 (26%), Positives = 80/191 (41%), Gaps = 3/191 (1%)
Frame = +2
Query: 2 FNILFFLIGAVILGVGIWVKVDKSNLFEVIRNIKLDDTTASFNNTNSTILDVVAIVLIIX 181
FN+LFF++GAVILG G+W+ DK++ V++ T+S+ L V A V I
Sbjct: 16 FNLLFFILGAVILGFGVWILADKNSFISVLQ-------------TSSSSLQVGAYVFIGV 62
Query: 182 XXXXXXXXXXXXXXAIKENKCLLITYSILXXXXXXXXXXXXXXXXXEKNKFQKNLGKSLT 361
A+ E +CLL Y + +K +K +G ++
Sbjct: 63 GAITIVMGFLGCIGAVNEVRCLLGLYFVFLLLILIAQVTVGVLFYFNADKLKKEMGNTVM 122
Query: 362 NVLEKDFNGILNTNATLF-TKIFTAIMFELKCCGVNNVTDFDSVRNWKSTSS--SGTKQI 532
+++ NAT + + + ++KCCG + S NW TK
Sbjct: 123 DIIRN-----YTANATSSREEAWDYVQAQVKCCG------WVSHYNWTENEELMGFTKTT 171
Query: 533 YPVGCCKLKEK 565
YP C K+KE+
Sbjct: 172 YPCSCEKIKEE 182
>sp|O60635|TSN1_HUMAN Tetraspanin-1 (Tspan-1) (Tetraspan NET-1) (Tetraspanin TM4-C)
Length = 241
Score = 59.7 bits (143), Expect = 9e-09
Identities = 44/183 (24%), Positives = 73/183 (39%)
Frame = +2
Query: 2 FNILFFLIGAVILGVGIWVKVDKSNLFEVIRNIKLDDTTASFNNTNSTILDVVAIVLIIX 181
FN+L FL GA +L VGIWV +D ++ ++ L + F N ++ +V +
Sbjct: 14 FNLLIFLCGAALLAVGIWVSIDGASFLKIFG--PLSSSAMQFVNVGYFLIAAGVVVFAL- 70
Query: 182 XXXXXXXXXXXXXXAIKENKCLLITYSILXXXXXXXXXXXXXXXXXEKNKFQKNLGKSLT 361
A E+KC L+T+ + + L +
Sbjct: 71 -------GFLGCYGAKTESKCALVTFFFILLLIFIAEVAAAVVALVYTTMAEHFLTLLVV 123
Query: 362 NVLEKDFNGILNTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWKSTSSSGTKQIYPV 541
++KD+ + FT+++ M LKCCG N TDF+ +K S+ +P
Sbjct: 124 PAIKKDYG-----SQEDFTQVWNTTMKGLKCCGFTNYTDFEDSPYFKENSA------FPP 172
Query: 542 GCC 550
CC
Sbjct: 173 FCC 175
>sp|Q96SJ8|TSN18_HUMAN Tetraspanin-18 (Tspan-18)
Length = 248
Score = 58.2 bits (139), Expect = 3e-08
Identities = 50/213 (23%), Positives = 85/213 (39%), Gaps = 4/213 (1%)
Frame = +2
Query: 2 FNILFFLIGAVILGVGIWVKVDKSNLFEVIRNIKLDDTTASFNNTNSTILDVVAIVLIIX 181
FN FL GA +L +GIWV VD + E++ + +L A +L+
Sbjct: 16 FNFFIFLGGACLLAIGIWVMVDPTGFREIV--------------AANPLLLTGAYILLAM 61
Query: 182 XXXXXXXXXXXXXXAIKENKCLLITYSILXXXXXXXXXXXXXXXXXEKNKFQKNLGKS-L 358
A++ENKCLL+ + + F++NL +
Sbjct: 62 GGLLFLLGFLGCCGAVRENKCLLLFFFLFILIIFLAELSAAILAFI----FRENLTREFF 117
Query: 359 TNVLEKDFNGILNTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWKSTSSSGTKQIYP 538
T L K + G N + +F+ + ++M CCGVN DF ++ + + P
Sbjct: 118 TKELTKHYQG--NNDTDVFSATWNSVMITFGCCGVNGPEDFKFASVFRLLTLDSEE--VP 173
Query: 539 VGCCKLKEKYQYDPI---RDSNIMMRKRILKRQ 628
CC+ +E D + R+ ++ R L +Q
Sbjct: 174 EACCR-REPQSRDGVLLSREECLLGRSLFLNKQ 205
>sp|Q58CY8|TSN18_BOVIN Tetraspanin-18 (Tspan-18)
Length = 249
Score = 57.8 bits (138), Expect = 3e-08
Identities = 51/213 (23%), Positives = 86/213 (40%), Gaps = 4/213 (1%)
Frame = +2
Query: 2 FNILFFLIGAVILGVGIWVKVDKSNLFEVIRNIKLDDTTASFNNTNSTILDVVAIVLIIX 181
FN FL GA +LG+GIWV VD + E++ + +L A +L+
Sbjct: 16 FNFFIFLGGACLLGIGIWVMVDPTGFREIV--------------AANPLLITGAYILLAM 61
Query: 182 XXXXXXXXXXXXXXAIKENKCLLITYSILXXXXXXXXXXXXXXXXXEKNKFQKNLGKS-L 358
A++ENKCLL+ + + F+ NL +
Sbjct: 62 GGLLFLLGFLGCCGAVRENKCLLLFFFLFILIIFLAELSAAILAFI----FRGNLTREFF 117
Query: 359 TNVLEKDFNGILNTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWKSTSSSGTKQIYP 538
T L K + G +T+ +F+ + ++M CCGVN DF ++ + + P
Sbjct: 118 TKELTKHYQGSNDTD--VFSATWNSVMITFGCCGVNGPEDFKYASVFRLLTLDSDE--VP 173
Query: 539 VGCCKLKEKYQYDPI---RDSNIMMRKRILKRQ 628
CC+ +E D + R+ ++ R L +Q
Sbjct: 174 EACCR-REPQSRDGVLLSREECLLGRDLFLNKQ 205
>sp|Q5RC27|TSN1_PONPY Tetraspanin-1 (Tspan-1)
Length = 241
Score = 55.8 bits (133), Expect = 1e-07
Identities = 43/183 (23%), Positives = 71/183 (38%)
Frame = +2
Query: 2 FNILFFLIGAVILGVGIWVKVDKSNLFEVIRNIKLDDTTASFNNTNSTILDVVAIVLIIX 181
FN+L FL GA +L VGIWV +D ++ ++ L + F N ++ +V +
Sbjct: 14 FNLLIFLCGAALLAVGIWVSIDGASFLKIFG--PLSSSAMQFVNVGYFLIAAGVVVFAL- 70
Query: 182 XXXXXXXXXXXXXXAIKENKCLLITYSILXXXXXXXXXXXXXXXXXEKNKFQKNLGKSLT 361
A E+KC L+T+ + + L +
Sbjct: 71 -------GFLGCYGAKTESKCALMTFFFILLLIFIAEVAAAVVALVYTTMAEHFLTLLVV 123
Query: 362 NVLEKDFNGILNTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWKSTSSSGTKQIYPV 541
++KD+ + FT+++ M LKCCG N TDF+ K + +P
Sbjct: 124 PAIKKDYG-----SQEDFTQVWNTTMKGLKCCGFTNYTDFEDSPYLKENHA------FPP 172
Query: 542 GCC 550
CC
Sbjct: 173 FCC 175
>sp|Q80WR1|TSN18_MOUSE Tetraspanin-18 (Tspan-18)
Length = 248
Score = 55.1 bits (131), Expect = 2e-07
Identities = 43/185 (23%), Positives = 77/185 (41%), Gaps = 1/185 (0%)
Frame = +2
Query: 2 FNILFFLIGAVILGVGIWVKVDKSNLFEVIRNIKLDDTTASFNNTNSTILDVVAIVLIIX 181
FN FL GA +LGVGIWV VD + E++ + +L A +++
Sbjct: 16 FNFFVFLGGACLLGVGIWVLVDPTGFREIV--------------ATNPLLTTGAYIVLAM 61
Query: 182 XXXXXXXXXXXXXXAIKENKCLLITYSILXXXXXXXXXXXXXXXXXEKNKFQKNLGKS-L 358
A++EN+CLL+ + + F+++L +
Sbjct: 62 GGLLFLLGFLGCCGAVRENRCLLLFFFLFILIIFLVELSAAILAFI----FREHLTREFF 117
Query: 359 TNVLEKDFNGILNTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWKSTSSSGTKQIYP 538
T L K + G + + +F+ + ++M CCGVN DF ++ + T+++ P
Sbjct: 118 TKELTKHYQG--DNDTDVFSATWNSVMITFGCCGVNGPEDFKLASVFRLLTLD-TEEV-P 173
Query: 539 VGCCK 553
CC+
Sbjct: 174 KACCR 178
>sp|O14817|TSN4_HUMAN Tetraspanin-4 (Tspan-4) (Transmembrane 4 superfamily member 7)
(Novel antigen 2) (NAG-2)
Length = 238
Score = 54.7 bits (130), Expect = 3e-07
Identities = 47/167 (28%), Positives = 68/167 (40%), Gaps = 2/167 (1%)
Frame = +2
Query: 2 FNILFFLIGAVILGVGIWVKVDKSNLFEVIRNIKLDDTTASFNNTNSTILDV-VAIVLII 178
FN+LF+L G +LGVGIW L T SF +S+ + A +LII
Sbjct: 16 FNLLFWLGGCGVLGVGIW----------------LAATQGSFATLSSSFPSLSAANLLII 59
Query: 179 XXXXXXXXXXXXXXXAIKENKCLLITYSILXXXXXXXXXXXXXXXXXEKNKFQKNLGKSL 358
AIKENKCLL+T+ +L +K + + L
Sbjct: 60 TGAFVMAIGFVGCLGAIKENKCLLLTFFLLLLLVFLLEATIAILFFAYTDKIDRYAQQDL 119
Query: 359 TNVLEKDFNGILNTNATL-FTKIFTAIMFELKCCGVNNVTDFDSVRN 496
L + T + T ++ I + +CCGV+N TD+ V N
Sbjct: 120 KKGLH-----LYGTQGNVGLTNAWSIIQTDFRCCGVSNYTDWFEVYN 161
>sp|P27701|CD82_HUMAN CD82 antigen (Inducible membrane protein R2) (C33 antigen) (IA4)
(Metastasis suppressor Kangai 1) (Suppressor of
tumorigenicity-6) (Tetraspanin-27) (Tspan-27)
Length = 267
Score = 54.7 bits (130), Expect = 3e-07
Identities = 43/184 (23%), Positives = 75/184 (40%), Gaps = 2/184 (1%)
Frame = +2
Query: 2 FNILFFLIGAVILGVGIWVKVDKSNLFEVIRNIKLDDTTASFNNTNSTILDVVAIVLIIX 181
FN++FF++GAVILG G+W+ DKS+ V++ T+S+ L + A V I
Sbjct: 16 FNLIFFILGAVILGFGVWILADKSSFISVLQ-------------TSSSSLRMGAYVFIGV 62
Query: 182 XXXXXXXXXXXXXXAIKENKCLLITYSILXXXXXXXXXXXXXXXXXEKNKFQKNLGKSLT 361
A+ E +CLL Y K ++ +G +T
Sbjct: 63 GAVTMLMGFLGCIGAVNEVRCLLGLYFAFLLLILIAQVTAGALFYFNMGKLKQEMGGIVT 122
Query: 362 NVLEKDFNGILNTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWKSTSS--SGTKQIY 535
++ +D+N ++ + + ++KCCG + S NW + + + Y
Sbjct: 123 ELI-RDYN---SSREDSLQDAWDYVQAQVKCCG------WVSFYNWTDNAELMNRPEVTY 172
Query: 536 PVGC 547
P C
Sbjct: 173 PCSC 176
>sp|Q9DCK3|TSN4_MOUSE Tetraspanin-4 (Tspan-4) (Transmembrane 4 superfamily member 7)
Length = 238
Score = 54.7 bits (130), Expect = 3e-07
Identities = 43/166 (25%), Positives = 70/166 (42%), Gaps = 1/166 (0%)
Frame = +2
Query: 2 FNILFFLIGAVILGVGIWVKVDKSNLFEVIRNIKLDDTTASFNNTNSTILDVVAIVLIIX 181
FN+LF+L G +LGVGIW+ + N + ++SF + ++ A +LI+
Sbjct: 16 FNLLFWLGGCGVLGVGIWLAATQGNFATL---------SSSFPSLSA------ANLLIVT 60
Query: 182 XXXXXXXXXXXXXXAIKENKCLLITYSILXXXXXXXXXXXXXXXXXEKNKFQKNLGKSLT 361
A+KENKCLL+T+ +L +K + L
Sbjct: 61 GTFVMAIGFVGCIGALKENKCLLLTFFVLLLLVFLLEATIAVLFFAYSDKIDSYAQQDLK 120
Query: 362 NVLEKDFNGILNTNATL-FTKIFTAIMFELKCCGVNNVTDFDSVRN 496
L + T + T ++ I + +CCGV+N TD+ V N
Sbjct: 121 KGLH-----LYGTQGNVGLTNAWSIIQTDFRCCGVSNYTDWFEVYN 161
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,168,774
Number of Sequences: 369166
Number of extensions: 1593313
Number of successful extensions: 3975
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3797
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3939
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7357347200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)