Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_024_H01
(608 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O75390|CISY_HUMAN Citrate synthase, mitochondrial precursor 295 6e-80
sp|P00889|CISY_PIG Citrate synthase, mitochondrial precursor 294 1e-79
sp|Q9CZU6|CISY_MOUSE Citrate synthase, mitochondrial precursor 288 1e-77
sp|P23007|CISY_CHICK Citrate synthase, mitochondrial 272 6e-73
sp|P34575|CISY_CAEEL Probable citrate synthase, mitochondri... 257 1e-68
sp|P51044|CISY_ASPNG Citrate synthase, mitochondrial precursor 230 2e-60
sp|O00098|CISY_EMENI Citrate synthase, mitochondrial precursor 229 3e-60
sp|P00890|CISY1_YEAST Citrate synthase, mitochondrial precu... 223 3e-58
sp|Q10306|CISY_SCHPO Probable citrate synthase, mitochondri... 218 1e-56
sp|P79024|CISY_CANTR Citrate synthase, mitochondrial precursor 216 5e-56
>sp|O75390|CISY_HUMAN Citrate synthase, mitochondrial precursor
Length = 466
Score = 295 bits (755), Expect = 6e-80
Identities = 138/192 (71%), Positives = 168/192 (87%)
Frame = +3
Query: 33 ASTVNLKDILADTITKEQKKVSEFRKQHGETKVGEVTINMMYGGMRGIKGLVTETSVLDP 212
AS+ NLKDILAD I KEQ ++ FR+QHG+T VG++T++MMYGGMRG+KGLV ETSVLDP
Sbjct: 28 ASSTNLKDILADLIPKEQARIKTFRQQHGKTVVGQITVDMMYGGMRGMKGLVYETSVLDP 87
Query: 213 EEGIRFRGYSIPECQKVLPKSQGGEQPLPEGLFFLLLTGKIPTEEQVKWISKEWASRADL 392
+EGIRFRG+SIPECQK+LPK++GGE+PLPEGLF+LL+TG IPTEEQV W+SKEWA RA L
Sbjct: 88 DEGIRFRGFSIPECQKLLPKAKGGEEPLPEGLFWLLVTGHIPTEEQVSWLSKEWAKRAAL 147
Query: 393 PGHVVSMINNFPSSLHPMSQLVAASAALNSESKFVKAYHEGVSKSVYWETVYEDSMDLIA 572
P HVV+M++NFP++LHPMSQL AA ALNSES F +AY +G+S++ YWE +YEDSMDLIA
Sbjct: 148 PSHVVTMLDNFPTNLHPMSQLSAAVTALNSESNFARAYAQGISRTKYWELIYEDSMDLIA 207
Query: 573 KLSTVAAMIYRN 608
KL VAA IYRN
Sbjct: 208 KLPCVAAKIYRN 219
>sp|P00889|CISY_PIG Citrate synthase, mitochondrial precursor
Length = 464
Score = 294 bits (753), Expect = 1e-79
Identities = 138/192 (71%), Positives = 166/192 (86%)
Frame = +3
Query: 33 ASTVNLKDILADTITKEQKKVSEFRKQHGETKVGEVTINMMYGGMRGIKGLVTETSVLDP 212
AS+ NLKDILAD I KEQ ++ FR+QHG T VG++T++MMYGGMRG+KGLV ETSVLDP
Sbjct: 28 ASSTNLKDILADLIPKEQARIKTFRQQHGNTVVGQITVDMMYGGMRGMKGLVYETSVLDP 87
Query: 213 EEGIRFRGYSIPECQKVLPKSQGGEQPLPEGLFFLLLTGKIPTEEQVKWISKEWASRADL 392
+EGIRFRGYSIPECQK+LPK++GGE+PLPEGLF+LL+TG+IPTEEQV W+SKEWA RA L
Sbjct: 88 DEGIRFRGYSIPECQKMLPKAKGGEEPLPEGLFWLLVTGQIPTEEQVSWLSKEWAKRAAL 147
Query: 393 PGHVVSMINNFPSSLHPMSQLVAASAALNSESKFVKAYHEGVSKSVYWETVYEDSMDLIA 572
P HVV+M++NFP++LHPMSQL AA ALNSES F +AY EG+ ++ YWE +YED MDLIA
Sbjct: 148 PSHVVTMLDNFPTNLHPMSQLSAAITALNSESNFARAYAEGIHRTKYWELIYEDCMDLIA 207
Query: 573 KLSTVAAMIYRN 608
KL VAA IYRN
Sbjct: 208 KLPCVAAKIYRN 219
>sp|Q9CZU6|CISY_MOUSE Citrate synthase, mitochondrial precursor
Length = 464
Score = 288 bits (736), Expect = 1e-77
Identities = 132/192 (68%), Positives = 168/192 (87%)
Frame = +3
Query: 33 ASTVNLKDILADTITKEQKKVSEFRKQHGETKVGEVTINMMYGGMRGIKGLVTETSVLDP 212
AS+ NLKD+L++ I KEQ ++ F++QHG+T VG++T++MMYGGMRG+KGLV ETSVLDP
Sbjct: 28 ASSTNLKDVLSNLIPKEQARIKTFKQQHGKTVVGQITVDMMYGGMRGMKGLVYETSVLDP 87
Query: 213 EEGIRFRGYSIPECQKVLPKSQGGEQPLPEGLFFLLLTGKIPTEEQVKWISKEWASRADL 392
+EGIRFRGYSIPECQK+LPK++GGE+PLPEGLF+LL+TG++PTEEQV W+S+EWA RA L
Sbjct: 88 DEGIRFRGYSIPECQKMLPKAKGGEEPLPEGLFWLLVTGQMPTEEQVSWLSREWAKRAAL 147
Query: 393 PGHVVSMINNFPSSLHPMSQLVAASAALNSESKFVKAYHEGVSKSVYWETVYEDSMDLIA 572
P HVV+M++NFP++LHPMSQL AA ALNSES F +AY EG++++ YWE +YED MDLIA
Sbjct: 148 PSHVVTMLDNFPTNLHPMSQLSAAITALNSESNFARAYAEGMNRAKYWELIYEDCMDLIA 207
Query: 573 KLSTVAAMIYRN 608
KL VAA IYRN
Sbjct: 208 KLPCVAAKIYRN 219
>sp|P23007|CISY_CHICK Citrate synthase, mitochondrial
Length = 433
Score = 272 bits (695), Expect = 6e-73
Identities = 129/192 (67%), Positives = 158/192 (82%)
Frame = +3
Query: 33 ASTVNLKDILADTITKEQKKVSEFRKQHGETKVGEVTINMMYGGMRGIKGLVTETSVLDP 212
AS+ NLKD+LA I KEQ ++ FR+QHG T +G++T++M YGGMRG+KGLV ETSVLDP
Sbjct: 1 ASSTNLKDVLAALIPKEQARIKTFRQQHGGTALGQITVDMSYGGMRGMKGLVYETSVLDP 60
Query: 213 EEGIRFRGYSIPECQKVLPKSQGGEQPLPEGLFFLLLTGKIPTEEQVKWISKEWASRADL 392
+EGIRFRG+SIPECQK+LPK G +PLPEGLF+LL+TG+IPT QV W+SKEWA RA L
Sbjct: 61 DEGIRFRGFSIPECQKLLPKGGXGGEPLPEGLFWLLVTGQIPTGAQVSWLSKEWAKRAAL 120
Query: 393 PGHVVSMINNFPSSLHPMSQLVAASAALNSESKFVKAYHEGVSKSVYWETVYEDSMDLIA 572
P HVV+M++NFP++LHPMSQL AA ALNSES F +AY EG+ ++ YWE VYE +MDLIA
Sbjct: 121 PSHVVTMLDNFPTNLHPMSQLSAAITALNSESNFARAYAEGILRTKYWEMVYESAMDLIA 180
Query: 573 KLSTVAAMIYRN 608
KL VAA IYRN
Sbjct: 181 KLPCVAAKIYRN 192
>sp|P34575|CISY_CAEEL Probable citrate synthase, mitochondrial precursor
Length = 468
Score = 257 bits (657), Expect = 1e-68
Identities = 122/190 (64%), Positives = 152/190 (80%)
Frame = +3
Query: 39 TVNLKDILADTITKEQKKVSEFRKQHGETKVGEVTINMMYGGMRGIKGLVTETSVLDPEE 218
+ NLK++L+ I KV FR +HG T V V I+M+YGGMR +KG+VTETSVLDPEE
Sbjct: 32 STNLKEVLSKKIPAHNAKVKSFRTEHGSTVVQNVNIDMIYGGMRSMKGMVTETSVLDPEE 91
Query: 219 GIRFRGYSIPECQKVLPKSQGGEQPLPEGLFFLLLTGKIPTEEQVKWISKEWASRADLPG 398
GIRFRGYSIPECQK+LPK++GGE+PLPE +++LL TG +P+E Q I+KEW +RADLP
Sbjct: 92 GIRFRGYSIPECQKLLPKAKGGEEPLPEAIWWLLCTGDVPSEAQTAAITKEWNARADLPT 151
Query: 399 HVVSMINNFPSSLHPMSQLVAASAALNSESKFVKAYHEGVSKSVYWETVYEDSMDLIAKL 578
HVV M++NFP +LHPM+Q +AA AALN+ESKF AY GV+K+ YWE YEDSMDL+AKL
Sbjct: 152 HVVRMLDNFPDNLHPMAQFIAAIAALNNESKFAGAYARGVAKASYWEYAYEDSMDLLAKL 211
Query: 579 STVAAMIYRN 608
TVAA+IYRN
Sbjct: 212 PTVAAIIYRN 221
>sp|P51044|CISY_ASPNG Citrate synthase, mitochondrial precursor
Length = 475
Score = 230 bits (587), Expect = 2e-60
Identities = 112/199 (56%), Positives = 146/199 (73%)
Frame = +3
Query: 12 GIRNVRFASTVNLKDILADTITKEQKKVSEFRKQHGETKVGEVTINMMYGGMRGIKGLVT 191
G+R +LK+ A+ + E +KV + RK+HG +GEVT++ YGG RG+K LV
Sbjct: 30 GLRCYSTGKAKSLKETFAEKLPAELEKVKKLRKEHGSKVIGEVTLDQAYGGARGVKCLVW 89
Query: 192 ETSVLDPEEGIRFRGYSIPECQKVLPKSQGGEQPLPEGLFFLLLTGKIPTEEQVKWISKE 371
E SVLD EEGIRFRG +IPECQ++LPK+ GG++PLPEGLF+LLLTG+IPTE+QV+ +S E
Sbjct: 90 EGSVLDSEEGIRFRGRTIPECQELLPKAPGGQEPLPEGLFWLLLTGEIPTEQQVRDLSAE 149
Query: 372 WASRADLPGHVVSMINNFPSSLHPMSQLVAASAALNSESKFVKAYHEGVSKSVYWETVYE 551
WA+R+DLP + +I+ PS+LHPMSQ A AL ES F KAY +G++K YW +E
Sbjct: 150 WAARSDLPKFIEELIDRCPSTLHPMSQFSLAVTALEHESAFAKAYAKGINKKDYWNYTFE 209
Query: 552 DSMDLIAKLSTVAAMIYRN 608
DSMDLIAKL T+AA IYRN
Sbjct: 210 DSMDLIAKLPTIAAKIYRN 228
>sp|O00098|CISY_EMENI Citrate synthase, mitochondrial precursor
Length = 474
Score = 229 bits (585), Expect = 3e-60
Identities = 111/199 (55%), Positives = 146/199 (73%)
Frame = +3
Query: 12 GIRNVRFASTVNLKDILADTITKEQKKVSEFRKQHGETKVGEVTINMMYGGMRGIKGLVT 191
G+R T +LK+ AD + E +KV + RK+HG +GE+T++ YGG RG+K LV
Sbjct: 30 GLRCYSTGKTKSLKETFADKLPGELEKVKKLRKEHGNKVIGELTLDQAYGGARGVKCLVW 89
Query: 192 ETSVLDPEEGIRFRGYSIPECQKVLPKSQGGEQPLPEGLFFLLLTGKIPTEEQVKWISKE 371
E SVLD EEGIRFRG +IPECQK+LPK+ GGE+PLPEGLF+LLLTG++P+E+QV+ +S E
Sbjct: 90 EGSVLDSEEGIRFRGLTIPECQKLLPKAPGGEEPLPEGLFWLLLTGEVPSEQQVRDLSAE 149
Query: 372 WASRADLPGHVVSMINNFPSSLHPMSQLVAASAALNSESKFVKAYHEGVSKSVYWETVYE 551
WA+R+DLP + +I+ PS+LHPM+Q A AL ES F KA +G++K YW +E
Sbjct: 150 WAARSDLPKFIEELIDRVPSTLHPMAQFSLAVTALEHESAFAKATAKGINKKEYWHYTFE 209
Query: 552 DSMDLIAKLSTVAAMIYRN 608
DSMDLIAKL T+AA IYRN
Sbjct: 210 DSMDLIAKLPTIAAKIYRN 228
>sp|P00890|CISY1_YEAST Citrate synthase, mitochondrial precursor
Length = 479
Score = 223 bits (568), Expect = 3e-58
Identities = 106/197 (53%), Positives = 145/197 (73%)
Frame = +3
Query: 18 RNVRFASTVNLKDILADTITKEQKKVSEFRKQHGETKVGEVTINMMYGGMRGIKGLVTET 197
R+ AS LK+ A+ I + +++ +F+K+HG+T +GEV + YGGMRGIKGLV E
Sbjct: 35 RHYSSASEQTLKERFAEIIPAKAEEIKKFKKEHGKTVIGEVLLEQAYGGMRGIKGLVWEG 94
Query: 198 SVLDPEEGIRFRGYSIPECQKVLPKSQGGEQPLPEGLFFLLLTGKIPTEEQVKWISKEWA 377
SVLDPEEGIRFRG +IPE Q+ LPK++G +PLPE LF+LLLTG+IPT+ QVK +S + A
Sbjct: 95 SVLDPEEGIRFRGRTIPEIQRELPKAEGSTEPLPEALFWLLLTGEIPTDAQVKALSADLA 154
Query: 378 SRADLPGHVVSMINNFPSSLHPMSQLVAASAALNSESKFVKAYHEGVSKSVYWETVYEDS 557
+R+++P HV+ ++++ P LHPM+Q A AL SESKF KAY +GVSK YW +EDS
Sbjct: 155 ARSEIPEHVIQLLDSLPKDLHPMAQFSIAVTALESESKFAKAYAQGVSKKEYWSYTFEDS 214
Query: 558 MDLIAKLSTVAAMIYRN 608
+DL+ KL +A+ IYRN
Sbjct: 215 LDLLGKLPVIASKIYRN 231
>sp|Q10306|CISY_SCHPO Probable citrate synthase, mitochondrial precursor
Length = 473
Score = 218 bits (555), Expect = 1e-56
Identities = 104/188 (55%), Positives = 137/188 (72%)
Frame = +3
Query: 45 NLKDILADTITKEQKKVSEFRKQHGETKVGEVTINMMYGGMRGIKGLVTETSVLDPEEGI 224
+LKD LA+ I ++Q ++ +FR +HG+ +GEVTIN MYGG RG++ L+ E SVLDP EGI
Sbjct: 41 SLKDRLAELIPEKQAEIKKFRAEHGQDVIGEVTINQMYGGARGVRSLIWEGSVLDPNEGI 100
Query: 225 RFRGYSIPECQKVLPKSQGGEQPLPEGLFFLLLTGKIPTEEQVKWISKEWASRADLPGHV 404
RFRGY+IPECQK+LP S G+QPLPE LF+LL+TG+IPT QV+ +S +WA+R+ LP V
Sbjct: 101 RFRGYTIPECQKLLPSSPNGKQPLPESLFWLLVTGEIPTLSQVQALSADWAARSQLPKFV 160
Query: 405 VSMINNFPSSLHPMSQLVAASAALNSESKFVKAYHEGVSKSVYWETVYEDSMDLIAKLST 584
+I+ P +LHPM+Q A AL +S F KAY G++K YW+ YED MDLIAK
Sbjct: 161 EELIDRCPPTLHPMAQFSLAVTALEHDSAFAKAYERGMNKHDYWKYEYEDCMDLIAKTVP 220
Query: 585 VAAMIYRN 608
+A IYRN
Sbjct: 221 IAGRIYRN 228
>sp|P79024|CISY_CANTR Citrate synthase, mitochondrial precursor
Length = 467
Score = 216 bits (549), Expect = 5e-56
Identities = 106/187 (56%), Positives = 140/187 (74%)
Frame = +3
Query: 48 LKDILADTITKEQKKVSEFRKQHGETKVGEVTINMMYGGMRGIKGLVTETSVLDPEEGIR 227
LK L + + + ++V + +K +G+T +GEV + YGGMRGIKGLV E SVLDP EGIR
Sbjct: 32 LKQRLEEILPAKAEEVKQLKKDYGKTVIGEVLLEQAYGGMRGIKGLVWEGSVLDPIEGIR 91
Query: 228 FRGYSIPECQKVLPKSQGGEQPLPEGLFFLLLTGKIPTEEQVKWISKEWASRADLPGHVV 407
FRG +IP+ QK LPK+ GGE+PLPE LF+LLLTG++PTE Q + +S+E+A+R+ LP HV
Sbjct: 92 FRGRTIPDIQKELPKAPGGEEPLPEALFWLLLTGEVPTEAQTRALSEEFAARSALPKHVE 151
Query: 408 SMINNFPSSLHPMSQLVAASAALNSESKFVKAYHEGVSKSVYWETVYEDSMDLIAKLSTV 587
+I+ PS LHPM+Q A AL SES+F KAY +GV KS YW+ YEDS++L+AKL T+
Sbjct: 152 ELIDRSPSHLHPMAQFSIAVTALESESQFAKAYAKGVHKSEYWKYTYEDSIELLAKLPTI 211
Query: 588 AAMIYRN 608
AA IYRN
Sbjct: 212 AAKIYRN 218
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 70,500,279
Number of Sequences: 369166
Number of extensions: 1433708
Number of successful extensions: 3485
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3396
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3467
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4748907085
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)