Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_024_F14
(858 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q28709|CD63_RABIT CD63 antigen 75 3e-13
sp|P08962|CD63_HUMAN CD63 antigen (Melanoma-associated anti... 71 4e-12
sp|P48509|CD151_HUMAN Platelet-endothelial tetraspan antige... 70 6e-12
sp|P28648|CD63_RAT CD63 antigen (AD1 antigen) 70 7e-12
sp|Q9QZA6|CD151_RAT Platelet endothelial tetraspan antigen ... 70 1e-11
sp|O35566|C151_MOUSE Platelet-endothelial tetraspan antigen... 69 1e-11
sp|P41731|CD63_MOUSE CD63 antigen 66 1e-10
sp|P61171|CD151_MACMU Platelet-endothelial tetraspan antige... 65 2e-10
sp|Q9XSK2|CD63_BOVIN CD63 antigen 65 3e-10
sp|O70401|TSN6_MOUSE Tetraspanin-6 (Tspan-6) (Transmembrane... 65 3e-10
>sp|Q28709|CD63_RABIT CD63 antigen
Length = 238
Score = 74.7 bits (182), Expect = 3e-13
Identities = 37/128 (28%), Positives = 62/128 (48%)
Frame = +3
Query: 408 VYKNSVEGRITDGLKEKIRNNYEINTDTAVLIDSLQKNFLCCGGDSKDDWKESKYSNQTK 587
V+++ V +++++N Y + TA+++D +QK+F CCG + DW + T+
Sbjct: 106 VFRDKVMSEFNKDFRQQMQN-YSTDNQTALILDRMQKDFTCCGAANYTDW--ATIPGMTR 162
Query: 588 QRYPSSCCKTSNVTCWNDENIKDVYEDGCRXXXXXXXXXXXXXXXGVLLGIAFFEMFVVI 767
R P SCC C N+KD+Y +GC LGIAF E+ ++
Sbjct: 163 DRVPDSCCVNVTSGCGVKFNVKDIYVEGCVEKIGLWLRKNVLVVAAAALGIAFVEVLGIV 222
Query: 768 CAFCLCRN 791
A CL ++
Sbjct: 223 FACCLVKS 230
>sp|P08962|CD63_HUMAN CD63 antigen (Melanoma-associated antigen ME491)
(Lysosome-associated membrane glycoprotein 3) (LAMP-3)
(Ocular melanoma-associated antigen) (OMA81H)
(Granulophysin) (Tetraspanin-30) (Tspan-30)
Length = 238
Score = 70.9 bits (172), Expect = 4e-12
Identities = 37/128 (28%), Positives = 63/128 (49%)
Frame = +3
Query: 408 VYKNSVEGRITDGLKEKIRNNYEINTDTAVLIDSLQKNFLCCGGDSKDDWKESKYSNQTK 587
V+++ V + ++++ N Y N TA ++D +Q +F CCG + DW+ K + +K
Sbjct: 106 VFRDKVMSEFNNNFRQQMEN-YPKNNHTASILDRMQADFKCCGAANYTDWE--KIPSMSK 162
Query: 588 QRYPSSCCKTSNVTCWNDENIKDVYEDGCRXXXXXXXXXXXXXXXGVLLGIAFFEMFVVI 767
R P SCC V C + N K ++++GC LGIAF E+ ++
Sbjct: 163 NRVPDSCCINVTVGCGINFNEKAIHKEGCVEKIGGWLRKNVLVVAAAALGIAFVEVLGIV 222
Query: 768 CAFCLCRN 791
A CL ++
Sbjct: 223 FACCLVKS 230
>sp|P48509|CD151_HUMAN Platelet-endothelial tetraspan antigen 3 (PETA-3) (GP27) (Membrane
glycoprotein SFA-1) (CD151 antigen) (Tetraspanin-24)
(Tspan-24)
Length = 253
Score = 70.5 bits (171), Expect = 6e-12
Identities = 54/241 (22%), Positives = 95/241 (39%), Gaps = 9/241 (3%)
Frame = +3
Query: 96 CGPKLAKCILILFNLVFWLSGLIMIVIASMLLSSVTMKYVESI--GNFSASRSLLXXXXX 269
CG K +L +N FWL+GL ++ + L+ + Y+ + G + A+ +L
Sbjct: 11 CGTVCLKYLLFTYNCCFWLAGLAVMAVGIWTLA-LKSDYISLLASGTYLATAYILVVAGT 69
Query: 270 XXXXXXXXXXXXXXXAIRESKMXXXXXXXXXXXXXXXXXXXGIVVIVYKNSVEGRITDGL 449
+E + GI+ Y + + + L
Sbjct: 70 VVMVTGVLGCCA---TFKERRNLLRLYFILLLIIFLLEIIAGILAYAYYQQLNTELKENL 126
Query: 450 KEKIRNNYEINTDTAVL--IDSLQKNFLCCGGDSKDDWKESKYSNQTK---QRYPSSCCK 614
K+ + Y + AV +D LQ+ F CCG ++ DW++S++ + + P SCCK
Sbjct: 127 KDTMTRRYHQSGHEAVTSAVDQLQQEFHCCGSNNSQDWRDSEWIRSQEAGGRVVPDSCCK 186
Query: 615 TSNVTCWNDENIKDVY--EDGCRXXXXXXXXXXXXXXXGVLLGIAFFEMFVVICAFCLCR 788
T C ++ ++Y E GC V +GIA ++F +I CL R
Sbjct: 187 TVVALCGQRDHASNIYKVEGGCITKLETFIQEHLRVIGAVGIGIACVQVFGMIFTCCLYR 246
Query: 789 N 791
+
Sbjct: 247 S 247
>sp|P28648|CD63_RAT CD63 antigen (AD1 antigen)
Length = 238
Score = 70.1 bits (170), Expect = 7e-12
Identities = 37/128 (28%), Positives = 60/128 (46%)
Frame = +3
Query: 408 VYKNSVEGRITDGLKEKIRNNYEINTDTAVLIDSLQKNFLCCGGDSKDDWKESKYSNQTK 587
V+++ V+ + +++++N Y + TA ++D LQK CCG + DW+ + K
Sbjct: 106 VFRDQVKSEFSKSFQKQMQN-YLTDNKTATILDKLQKENKCCGASNYTDWE--RIPGMAK 162
Query: 588 QRYPSSCCKTSNVTCWNDENIKDVYEDGCRXXXXXXXXXXXXXXXGVLLGIAFFEMFVVI 767
R P SCC V C ND ++ GC G LGIAF E+ +I
Sbjct: 163 DRVPDSCCINITVGCGNDFKESTIHTQGCVETIAAWLRKNVLLVAGAALGIAFVEVLGII 222
Query: 768 CAFCLCRN 791
+ CL ++
Sbjct: 223 FSCCLVKS 230
>sp|Q9QZA6|CD151_RAT Platelet endothelial tetraspan antigen 3 (CD151 antigen)
Length = 253
Score = 69.7 bits (169), Expect = 1e-11
Identities = 55/239 (23%), Positives = 93/239 (38%), Gaps = 7/239 (2%)
Frame = +3
Query: 96 CGPKLAKCILILFNLVFWLSGLIMIVIASMLLSSVTMKYVESIGNFSASRSLLXXXXXXX 275
CG K +L +N FWL+GL ++ + L+ + Y+ + + S +
Sbjct: 11 CGTVCLKYLLFTYNCCFWLAGLAVMAVGIWTLA-LKSDYISLLAS-STYLATAYILVVAG 68
Query: 276 XXXXXXXXXXXXXAIRESKMXXXXXXXXXXXXXXXXXXXGIVVIVYKNSVEGRITDGLKE 455
+E + GI+ VY + + + LK+
Sbjct: 69 VVVMVTGVLGCCATFKERRNLLRLYFILLLIIFLLEIIAGILAYVYYQQLNTELKENLKD 128
Query: 456 KIRNNYEINTDTAVL--IDSLQKNFLCCGGDSKDDWKESKY--SNQTKQR-YPSSCCKTS 620
+ Y + V +D LQ+ F CCG ++ DW++S++ S + R P SCCKT
Sbjct: 129 TMIKRYHQSGHEGVTNAVDKLQQEFHCCGSNNSRDWRDSEWIRSGEADSRVVPDSCCKTV 188
Query: 621 NVTCWNDENIKDVY--EDGCRXXXXXXXXXXXXXXXGVLLGIAFFEMFVVICAFCLCRN 791
C E+ ++Y E GC V +GIA ++F +I CL R+
Sbjct: 189 VTGCGKREHASNIYKVEGGCITKLESFIQEHLRVIGAVGIGIACVQVFGMIFTCCLYRS 247
>sp|O35566|C151_MOUSE Platelet-endothelial tetraspan antigen 3 (PETA-3) (GP27) (Membrane
glycoprotein SFA-1) (CD151 antigen)
Length = 253
Score = 69.3 bits (168), Expect = 1e-11
Identities = 54/239 (22%), Positives = 93/239 (38%), Gaps = 7/239 (2%)
Frame = +3
Query: 96 CGPKLAKCILILFNLVFWLSGLIMIVIASMLLSSVTMKYVESIGNFSASRSLLXXXXXXX 275
CG K +L +N FWL+GL ++ + L+ + Y+ + + S +
Sbjct: 11 CGTVCLKYLLFTYNCCFWLAGLAVMAVGIWTLA-LKSDYISLLAS-STYLATAYILVVAG 68
Query: 276 XXXXXXXXXXXXXAIRESKMXXXXXXXXXXXXXXXXXXXGIVVIVYKNSVEGRITDGLKE 455
+E + GI+ VY + + + LK+
Sbjct: 69 VVVMVTGVLGCCATFKERRNLLRLYFILLLIIFLLEIIAGILAYVYYQQLNTELKENLKD 128
Query: 456 KIRNNYEINTDTAV--LIDSLQKNFLCCGGDSKDDWKESKY--SNQTKQR-YPSSCCKTS 620
+ Y + V +D LQ+ F CCG ++ DW++S++ S + R P SCCKT
Sbjct: 129 TMVKRYHQSGHEGVSSAVDKLQQEFHCCGSNNSQDWQDSEWIRSGEADSRVVPDSCCKTM 188
Query: 621 NVTCWNDENIKDVY--EDGCRXXXXXXXXXXXXXXXGVLLGIAFFEMFVVICAFCLCRN 791
C ++ ++Y E GC V +GIA ++F +I CL R+
Sbjct: 189 VAGCGKRDHASNIYKVEGGCITKLETFIQEHLRVIGAVGIGIACVQVFGMIFTCCLYRS 247
>sp|P41731|CD63_MOUSE CD63 antigen
Length = 238
Score = 65.9 bits (159), Expect = 1e-10
Identities = 36/128 (28%), Positives = 57/128 (44%)
Frame = +3
Query: 408 VYKNSVEGRITDGLKEKIRNNYEINTDTAVLIDSLQKNFLCCGGDSKDDWKESKYSNQTK 587
V+++ V+ +++++N Y + TA ++D LQK CCG + DW+ K
Sbjct: 106 VFRDQVKSEFNKSFQQQMQN-YLKDNKTATILDKLQKENNCCGASNYTDWE--NIPGMAK 162
Query: 588 QRYPSSCCKTSNVTCWNDENIKDVYEDGCRXXXXXXXXXXXXXXXGVLLGIAFFEMFVVI 767
R P SCC V C ND ++ GC LGIAF E+ +I
Sbjct: 163 DRVPDSCCINITVGCGNDFKESTIHTQGCVETIAIWLRKNILLVAAAALGIAFVEVLGII 222
Query: 768 CAFCLCRN 791
+ CL ++
Sbjct: 223 FSCCLVKS 230
>sp|P61171|CD151_MACMU Platelet-endothelial tetraspan antigen 3 (PETA-3) (CD151 antigen)
sp|P61170|CD151_CERAE Platelet-endothelial tetraspan antigen 3 (PETA-3) (CD151 antigen)
Length = 253
Score = 65.1 bits (157), Expect = 2e-10
Identities = 54/241 (22%), Positives = 93/241 (38%), Gaps = 9/241 (3%)
Frame = +3
Query: 96 CGPKLAKCILILFNLVFWLSGLIMIVIASMLLSSVTMKYVESI--GNFSASRSLLXXXXX 269
CG K +L +N FWL+GL ++ + L+ + Y+ + G + A+ +L
Sbjct: 11 CGTVCLKYLLFTYNCCFWLAGLAVMAVGIWTLA-LKSDYISLLASGTYLATAYILVVAGA 69
Query: 270 XXXXXXXXXXXXXXXAIRESKMXXXXXXXXXXXXXXXXXXXGIVVIVYKNSVEGRITDGL 449
+E + G++ VY + + + L
Sbjct: 70 VVMVTGVLGCCA---TFKERRNLLRLYFILLLIIFLLEIIAGVLAYVYYQQLNTELKENL 126
Query: 450 KEKIRNNYEINTDTAVL--IDSLQKNFLCCGGDSKDDWKESKYSNQTKQR---YPSSCCK 614
K+ + Y AV +D LQ+ F CCG ++ DW++S++ + R P SCCK
Sbjct: 127 KDTMAKRYHQPGHEAVTSAVDQLQQEFHCCGSNNSQDWRDSEWIRLREARGRVVPDSCCK 186
Query: 615 TSNVTCWNDENIKDVY--EDGCRXXXXXXXXXXXXXXXGVLLGIAFFEMFVVICAFCLCR 788
T C ++ ++Y E G V GIA ++F +I CL R
Sbjct: 187 TVVAGCGQRDHAFNIYKVEGGFITKLETFIQEHLRVIGAVGTGIACVQVFGMIFTCCLYR 246
Query: 789 N 791
+
Sbjct: 247 S 247
>sp|Q9XSK2|CD63_BOVIN CD63 antigen
Length = 237
Score = 64.7 bits (156), Expect = 3e-10
Identities = 33/128 (25%), Positives = 60/128 (46%)
Frame = +3
Query: 408 VYKNSVEGRITDGLKEKIRNNYEINTDTAVLIDSLQKNFLCCGGDSKDDWKESKYSNQTK 587
V+++ V +++++N Y + TA ++D +QK+F CCG + DW++
Sbjct: 106 VFRDKVRSEFNKDFRQQMKN-YPKDNQTASILDKMQKDFECCGAANYTDWEKIL---AVT 161
Query: 588 QRYPSSCCKTSNVTCWNDENIKDVYEDGCRXXXXXXXXXXXXXXXGVLLGIAFFEMFVVI 767
+ P SCC C + +KD++ +GC LGIAF E+ ++
Sbjct: 162 NKVPDSCCVNITHNCGINFVVKDIHTEGCVEKIAAWLRKNVLVVVAAALGIAFVEILGIV 221
Query: 768 CAFCLCRN 791
A CL ++
Sbjct: 222 LACCLVKS 229
>sp|O70401|TSN6_MOUSE Tetraspanin-6 (Tspan-6) (Transmembrane 4 superfamily member 6)
Length = 245
Score = 64.7 bits (156), Expect = 3e-10
Identities = 43/225 (19%), Positives = 87/225 (38%)
Frame = +3
Query: 114 KCILILFNLVFWLSGLIMIVIASMLLSSVTMKYVESIGNFSASRSLLXXXXXXXXXXXXX 293
K +L+++ +FW++G+I++ + + V+++ S+ N A+ ++
Sbjct: 18 KSVLLIYTFIFWITGVILLAVG--IWGKVSLENYFSLLNEKAT-NVPFVLIGTGTVIILL 74
Query: 294 XXXXXXXAIRESKMXXXXXXXXXXXXXXXXXXXGIVVIVYKNSVEGRITDGLKEKIRNNY 473
R S IV V+++ ++ + ++
Sbjct: 75 GTFGCFATCRTSAWMLKLYAMFLTLIFLVELVAAIVGFVFRHEIKNSFKSNYENALKEYN 134
Query: 474 EINTDTAVLIDSLQKNFLCCGGDSKDDWKESKYSNQTKQRYPSSCCKTSNVTCWNDENIK 653
+ +D +Q CCG + DWK + Y ++T +P SCCK C+ +
Sbjct: 135 STGDYRSEAVDKIQSTLHCCGVTNYGDWKGTNYYSET--GFPKSCCKLEG--CYPQRDAD 190
Query: 654 DVYEDGCRXXXXXXXXXXXXXXXGVLLGIAFFEMFVVICAFCLCR 788
V E+GC G+ G+A F++ + A+CL R
Sbjct: 191 KVNEEGCFIKVMTTIESEMGVVAGISFGVACFQLIGIFLAYCLSR 235
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 91,983,933
Number of Sequences: 369166
Number of extensions: 1821098
Number of successful extensions: 4405
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4238
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4375
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8486520240
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)