Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_024_D07
(817 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P53990|K0174_HUMAN Protein KIAA0174 (Putative MAPK-activ... 231 2e-60
sp|P53843|YN05_YEAST Hypothetical 34.5 kDa protein in PIK1-... 103 7e-22
sp|P08964|MYO1_YEAST Myosin-1 (Type II myosin) 30 7.9
sp|Q8RDD1|SYM_THETN Methionyl-tRNA synthetase (Methionine--... 30 7.9
sp|Q885J0|SYA_PSESM Alanyl-tRNA synthetase (Alanine--tRNA l... 30 7.9
sp|O81972|C82A2_SOYBN Cytochrome P450 82A2 (P450 CP4) 30 7.9
>sp|P53990|K0174_HUMAN Protein KIAA0174 (Putative MAPK-activating protein PM28)
Length = 364
Score = 231 bits (588), Expect = 2e-60
Identities = 108/180 (60%), Positives = 145/180 (80%)
Frame = +2
Query: 23 MFSSKPNYNKLKTNLRIVVNRLKLLEKKNTEIALKSRKEIADYLTSAKYDRARIKVEMII 202
M S +L+ NLR+V+NRLKLLEKK TE+A K+RKEIADYL + K +RARI+VE II
Sbjct: 1 MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60
Query: 203 RQDYLVEVYELIEMFCDLLLARFGLIECQKEIDPGLEEQIATLIWVTPRLQTDVQELKLI 382
R+DYLVE E++E++CDLLLARFGLI+ KE+D GL E ++TLIW PRLQ++V ELK++
Sbjct: 61 REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120
Query: 383 TDQLHLKYGREYGLACQSNSLNNVNEKVMEKLSIKAPPKRLVEQYLVEIAKSFDVPYEPE 562
DQL KY +EYG C++N + VN+++M KLS++APPK LVE+YL+EIAK+++VPYEP+
Sbjct: 121 ADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPD 180
>sp|P53843|YN05_YEAST Hypothetical 34.5 kDa protein in PIK1-POL2 intergenic region
Length = 298
Score = 103 bits (256), Expect = 7e-22
Identities = 70/216 (32%), Positives = 120/216 (55%), Gaps = 8/216 (3%)
Frame = +2
Query: 50 KLKTNLRIVVNRLKLLEKKNTEIALKSRKEIADYLTSAKYDRARIKVEMIIRQDYLVEVY 229
KLKT L++ + RL+ ++K IA +SR+++A L + K +A +VE +I D +E+
Sbjct: 11 KLKTCLKMCIQRLRYAQEKQQAIAKQSRRQVAQLLLTNKEQKAHYRVETLIHDDIHIELL 70
Query: 230 ELIEMFCDLLLARFGLIE--------CQKEIDPGLEEQIATLIWVTPRLQTDVQELKLIT 385
E++E++C+LLLAR +I ++ +D G+ E I +LI+ +V+EL +
Sbjct: 71 EILELYCELLLARVQVINDISTEEQLVKEHMDDGINEAIRSLIYAI-LFVDEVKELSQLK 129
Query: 386 DQLHLKYGREYGLACQSNSLNNVNEKVMEKLSIKAPPKRLVEQYLVEIAKSFDVPYEPEE 565
D + K E+ ++ + +V EK+++K S P + LV+ YL EIAK++DVPY E
Sbjct: 130 DLMAWKINVEFVNGVIADHI-DVPEKIIKKCSPSVPKEELVDLYLKEIAKTYDVPYSKLE 188
Query: 566 DIDPPASNWNPGEFYAPLPPNLIDFMGEKPPLINPN 673
+ +S+ +F P ++ D EKP L N
Sbjct: 189 NSLSSSSSNISSDFSDP-SGDIEDNDEEKPILALDN 223
>sp|P08964|MYO1_YEAST Myosin-1 (Type II myosin)
Length = 1928
Score = 30.0 bits (66), Expect = 7.9
Identities = 45/175 (25%), Positives = 74/175 (42%)
Frame = +2
Query: 47 NKLKTNLRIVVNRLKLLEKKNTEIALKSRKEIADYLTSAKYDRARIKVEMIIRQDYLVEV 226
NKLK +L+ + ++ K LE+KN + + + D L K + R ++ R E
Sbjct: 871 NKLKNDLQEMESKKKFLEEKNQK-TVNELENTQDLLNQEK-ENLRKNESLLNRVKTSSET 928
Query: 227 YELIEMFCDLLLARFGLIECQKEIDPGLEEQIATLIWVTPRLQTDVQELKLITDQLHLKY 406
L + F DL+ + + + E+ LEE + LQ ++E + ++LH K
Sbjct: 929 --LQKQFDDLVSEKDEISREKLEVAQNLEEAHQKI----QGLQETIREREATLEKLHSKN 982
Query: 407 GREYGLACQSNSLNNVNEKVMEKLSIKAPPKRLVEQYLVEIAKSFDVPYEPEEDI 571
L Q + LN K S+ K +E EI + DV EE+I
Sbjct: 983 NE---LIKQISDLNCDISKEQSSQSLIKESKLKLEN---EIKRLKDVINSKEEEI 1031
>sp|Q8RDD1|SYM_THETN Methionyl-tRNA synthetase (Methionine--tRNA ligase) (MetRS)
Length = 638
Score = 30.0 bits (66), Expect = 7.9
Identities = 16/57 (28%), Positives = 31/57 (54%)
Frame = +2
Query: 74 VVNRLKLLEKKNTEIALKSRKEIADYLTSAKYDRARIKVEMIIRQDYLVEVYELIEM 244
V L LEKK E ++++E DY+T + + +++V I+ + + +LI+M
Sbjct: 508 VEKELASLEKKTEEKTKETKEEKIDYITIEDFSKVQLRVAEILEAEKVEGSDKLIKM 564
>sp|Q885J0|SYA_PSESM Alanyl-tRNA synthetase (Alanine--tRNA ligase) (AlaRS)
Length = 874
Score = 30.0 bits (66), Expect = 7.9
Identities = 18/52 (34%), Positives = 27/52 (51%)
Frame = +2
Query: 35 KPNYNKLKTNLRIVVNRLKLLEKKNTEIALKSRKEIADYLTSAKYDRARIKV 190
K N + L L V+ R +LLEK+ ++ K+ D L+SA D +KV
Sbjct: 721 KGNRDNLLDKLTAVLERNRLLEKQLEQLQAKAASAAGDDLSSAALDVKGVKV 772
>sp|O81972|C82A2_SOYBN Cytochrome P450 82A2 (P450 CP4)
Length = 522
Score = 30.0 bits (66), Expect = 7.9
Identities = 28/104 (26%), Positives = 47/104 (45%), Gaps = 8/104 (7%)
Frame = +2
Query: 206 QDYLVEVYELIE------MFCDLLLARFGLIECQKEIDPGLEEQIATLIWVTPRLQTDVQ 367
QD + + L+E M D+++ F L Q G E I TLIW T + +
Sbjct: 284 QDLMSVLLSLLEGKTIEGMNVDIVIKSFVLTVIQA----GTEASITTLIWATSLILNNPS 339
Query: 368 ELKLITDQLHLKYGREYGLACQS--NSLNNVNEKVMEKLSIKAP 493
L+ + +L ++ G+E + C+S + L + V E L + P
Sbjct: 340 VLEKLKAELDIQVGKERYI-CESDLSKLTYLQAVVKETLRLYPP 382
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,186,724
Number of Sequences: 369166
Number of extensions: 1654704
Number of successful extensions: 3979
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 3821
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3975
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7811456130
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)