Planarian EST Database


Dr_sW_024_D05-2

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_024_D05-2
         (495 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P31939|PUR9_HUMAN  Bifunctional purine biosynthesis prote...    30   2.4  
sp|Q58347|PUR2_METJA  Phosphoribosylamine--glycine ligase (G...    30   2.4  
sp|Q01369|GBLP_NEUCR  Guanine nucleotide-binding protein bet...    30   3.1  
sp|Q94981|ARI1_DROME  Ariadne-1 protein (Ari-1)                    30   3.1  
sp|P54032|RS24_METJA  30S ribosomal protein S24e                   29   5.3  
sp|P32006|PROF1_STRPU  Profilin (SpCoel1)                          29   5.3  
sp|P27920|R1AB_IBVB  Replicase polyprotein 1ab (pp1ab) (ORF1...    29   5.3  
sp|Q91QT2|R1AB_IBVBC  Replicase polyprotein 1ab (pp1ab) (ORF...    29   5.3  
sp|Q12397|STCA_EMENI  Putative sterigmatocystin biosynthesis...    28   9.0  
sp|Q19020|DYHC_CAEEL  Dynein heavy chain, cytosolic (DYHC)         28   9.0  
>sp|P31939|PUR9_HUMAN Bifunctional purine biosynthesis protein PURH [Includes:
           Phosphoribosylaminoimidazolecarboxamide
           formyltransferase (AICAR transformylase); IMP
           cyclohydrolase (Inosinicase) (IMP synthetase) (ATIC)]
          Length = 592

 Score = 30.4 bits (67), Expect = 2.4
 Identities = 19/59 (32%), Positives = 32/59 (54%), Gaps = 3/59 (5%)
 Frame = +1

Query: 16  ILDGYMASGFTEINNITIASS--NGAVCISRSKEGPTPDANEIAALV-KYLTSKQGSGL 183
           + DG +A G+ E   +TI S   NG  C+ +  +   PD NE+  L   +L+ K+ +G+
Sbjct: 337 VSDGIIAPGYEE-EALTILSKKKNGNYCVLQMDQSYKPDENEVRTLFGLHLSQKRNNGV 394
>sp|Q58347|PUR2_METJA Phosphoribosylamine--glycine ligase (GARS) (Glycinamide
           ribonucleotide synthetase) (Phosphoribosylglycinamide
           synthetase)
          Length = 444

 Score = 30.4 bits (67), Expect = 2.4
 Identities = 18/47 (38%), Positives = 25/47 (53%)
 Frame = -2

Query: 146 NAAISFASGVGPSLLLLMHTAPLEEAIVILLISVKPDAI*PSKIAPQ 6
           +A   FA  V P L ++   APL E +V LL  +   A+ P K+A Q
Sbjct: 54  DAVKEFAEKVKPDLAVIGPEAPLGEGVVDLLEEMGISAVGPKKLAAQ 100
>sp|Q01369|GBLP_NEUCR Guanine nucleotide-binding protein beta subunit-like protein
           (Cross-pathway control WD-repeat protein cpc-2)
          Length = 316

 Score = 30.0 bits (66), Expect = 3.1
 Identities = 22/73 (30%), Positives = 35/73 (47%), Gaps = 2/73 (2%)
 Frame = +1

Query: 52  INNITIASSNGAVCISRSKEGPTP--DANEIAALVKYLTSKQGSGLTYGGHKLMFTREIE 225
           IN +TI S +G++C S  K+G T   D NE   L     + +   L +  ++        
Sbjct: 195 INAVTI-SPDGSLCASGGKDGTTMLWDLNESKHLYSLNANDEIHALVFSPNRYWLCAATS 253

Query: 226 DSIMIFNIIGASK 264
            SI+IF++   SK
Sbjct: 254 SSIIIFDLEKKSK 266
>sp|Q94981|ARI1_DROME Ariadne-1 protein (Ari-1)
          Length = 503

 Score = 30.0 bits (66), Expect = 3.1
 Identities = 10/18 (55%), Positives = 13/18 (72%)
 Frame = +3

Query: 273 CIEQNC*HQFCYSCLWSW 326
           C  QNC ++FC+ CL SW
Sbjct: 309 CKNQNCKNEFCWVCLGSW 326
>sp|P54032|RS24_METJA 30S ribosomal protein S24e
          Length = 101

 Score = 29.3 bits (64), Expect = 5.3
 Identities = 17/39 (43%), Positives = 24/39 (61%)
 Frame = -2

Query: 329 GPAPQARITKLVLAVLLNASAPLLAPIMLKIIIESSISR 213
           GP P  +  KL LA +LNA+  LL  I+ KI+ E+ + R
Sbjct: 27  GPTPTFKDVKLKLAAILNANKDLL--IVEKIVEEAGMQR 63
>sp|P32006|PROF1_STRPU Profilin (SpCoel1)
          Length = 142

 Score = 29.3 bits (64), Expect = 5.3
 Identities = 35/127 (27%), Positives = 55/127 (43%), Gaps = 19/127 (14%)
 Frame = +1

Query: 4   AWGAILDGYMA-----SGFTEINNITIASSNGAVCISRSKEGPTPDAN--------EIAA 144
           +W + +D  +A     SG T  +   I   +G+   +     PT D +        E+A 
Sbjct: 2   SWDSYVDNLIAQSKDASGTTHCDKACIIGKDGSAWTTM----PTSDTSNNLKLTPEEMAN 57

Query: 145 LVKYLTSKQ-----GSGLTYGGHKLMFTREIEDSIMIFNIIGASKGALALS-KTASTNFV 306
           + K   SK       SG+   G K  F RE EDS +   ++G  KG  +L+ +++ T  V
Sbjct: 58  IAKCFKSKDFAAFMSSGIYVNGTKYQFLRE-EDSKL---VLGKKKGEGSLTLQSSKTAIV 113

Query: 307 ILACGAG 327
           I  C  G
Sbjct: 114 IGHCPEG 120
>sp|P27920|R1AB_IBVB Replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) [Includes:
           Replicase polyprotein 1a (pp1a) (ORF1a)] [Contains: p87;
           p195 (Papain-like proteinase) (PL-PRO); Peptide HD2
           (p41); 3C-like proteinase (3CL-PRO) (3CLp) (M-PRO)
           (p33); p34; p9; p24; p10; Growth factor-like peptide
           (GFL) (p16); RNA-directed RNA polymerase (RdRp) (Pol)
           (p100); Helicase (Hel) (p68); p58; p39; p35]
          Length = 6629

 Score = 29.3 bits (64), Expect = 5.3
 Identities = 23/93 (24%), Positives = 38/93 (40%), Gaps = 22/93 (23%)
 Frame = +1

Query: 7   WGAILDGYMASGFTEINNITIASSNG------------AVCISRSKEGPTPDANEIAALV 150
           W AILDG++   F   +    A  +G             VC+S   +G TP+ N+   L 
Sbjct: 330 WSAILDGHLCYVFRSGDRFYAAPLSGNFALSDVHCCERVVCLS---DGVTPEINDGLILA 386

Query: 151 KYLTS----------KQGSGLTYGGHKLMFTRE 219
              +S          K+G    + GHK ++ ++
Sbjct: 387 AIYSSFSVSELVTALKKGEPFKFLGHKFVYAKD 419
>sp|Q91QT2|R1AB_IBVBC Replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) [Includes:
           Replicase polyprotein 1a (pp1a) (ORF1a)] [Contains: p87;
           p195 (Papain-like proteinase) (PL-PRO); Peptide HD2
           (p41); 3C-like proteinase (3CL-PRO) (3CLp) (M-PRO)
           (p33); p34; p9; p24; p10; Growth factor-like peptide
           (GFL) (p16); RNA-directed RNA polymerase (RdRp) (Pol)
           (p100); Helicase (Hel) (p68); p58; p39; p35]
          Length = 6629

 Score = 29.3 bits (64), Expect = 5.3
 Identities = 23/93 (24%), Positives = 38/93 (40%), Gaps = 22/93 (23%)
 Frame = +1

Query: 7   WGAILDGYMASGFTEINNITIASSNG------------AVCISRSKEGPTPDANEIAALV 150
           W AILDG++   F   +    A  +G             VC+S   +G TP+ N+   L 
Sbjct: 330 WSAILDGHLCYVFRSGDRFYAAPLSGNFALSDVHCCERVVCLS---DGVTPEINDGLILA 386

Query: 151 KYLTS----------KQGSGLTYGGHKLMFTRE 219
              +S          K+G    + GHK ++ ++
Sbjct: 387 AIYSSFSVSELVTALKKGEPFKFLGHKFVYAKD 419
>sp|Q12397|STCA_EMENI Putative sterigmatocystin biosynthesis polyketide synthase (PKS)
          Length = 2181

 Score = 28.5 bits (62), Expect = 9.0
 Identities = 16/46 (34%), Positives = 22/46 (47%)
 Frame = +1

Query: 52  INNITIASSNGAVCISRSKEGPTPDANEIAALVKYLTSKQGSGLTY 189
           INN + A  N AV +   ++ P P+  EIA         Q S L+Y
Sbjct: 796 INNFSAAGGNTAVLV---EDAPVPELGEIAGSFGEAAGNQSSELSY 838
>sp|Q19020|DYHC_CAEEL Dynein heavy chain, cytosolic (DYHC)
          Length = 4568

 Score = 28.5 bits (62), Expect = 9.0
 Identities = 13/26 (50%), Positives = 17/26 (65%)
 Frame = -1

Query: 462 RIKINIEK*YNPNELFSINANYNDLF 385
           R+K  +E   N NE+FSI + YN LF
Sbjct: 549 RLKSQLESSRNSNEMFSIFSRYNALF 574
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53,478,921
Number of Sequences: 369166
Number of extensions: 966458
Number of successful extensions: 2490
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2447
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2489
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 3069744620
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)