Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_024_B16
(565 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8NBX0|SCPDH_HUMAN Probable saccharopine dehydrogenase 115 7e-26
sp|Q5R5C9|SCPDH_PONPY Probable saccharopine dehydrogenase 114 2e-25
sp|Q8R127|SCPDH_MOUSE Probable saccharopine dehydrogenase 108 9e-24
sp|Q6AY30|SCPDH_RAT Probable saccharopine dehydrogenase 107 3e-23
sp|Q8D2J7|SUFS_WIGBR Cysteine desulfurase (Selenocysteine l... 31 2.4
sp|Q9D666|UN84A_MOUSE Sad1/unc-84 protein-like 1 (Unc-84 ho... 30 5.4
>sp|Q8NBX0|SCPDH_HUMAN Probable saccharopine dehydrogenase
Length = 429
Score = 115 bits (288), Expect = 7e-26
Identities = 60/140 (42%), Positives = 84/140 (60%)
Frame = +2
Query: 20 MFIGGIFALFSNFGIGRTLLLKFPRFFSCGIVSFDGASRKAIENSKMELILIGRGWSNHS 199
MF G F F FGIGR LL+KFP FFS G S G ++K I+ + L G+G+S +
Sbjct: 290 MFAGLFFLFFVRFGIGRQLLIKFPWFFSFGYFSKQGPTQKQIDAASFTLTFFGQGYSQGT 349
Query: 200 SVDVEKPNRKIVCSISGPEPVYWSCSIFIVQAGLTILKEQSKMPGNGGVYSPLAAFNNTT 379
D KPN KI + GPE Y + I +VQA +T+L + S +P GGV++P AAF+ T
Sbjct: 350 GTDKNKPNIKICTQVKGPEAGYVATPIAMVQAAMTLLSDASHLPKAGGVFTPGAAFSKTK 409
Query: 380 YLERLQRNEINFAKISDTSV 439
++RL ++ I F+ IS + V
Sbjct: 410 LIDRLNKHGIEFSVISSSEV 429
>sp|Q5R5C9|SCPDH_PONPY Probable saccharopine dehydrogenase
Length = 429
Score = 114 bits (285), Expect = 2e-25
Identities = 60/140 (42%), Positives = 83/140 (59%)
Frame = +2
Query: 20 MFIGGIFALFSNFGIGRTLLLKFPRFFSCGIVSFDGASRKAIENSKMELILIGRGWSNHS 199
MF G F F FGIGR LL+KFP FFS G S G ++K I+ + L G+G+S
Sbjct: 290 MFAGLFFLFFVRFGIGRQLLIKFPWFFSFGYFSKQGPTQKQIDAASFTLTFFGQGYSQGI 349
Query: 200 SVDVEKPNRKIVCSISGPEPVYWSCSIFIVQAGLTILKEQSKMPGNGGVYSPLAAFNNTT 379
D KPN KI + GPE Y + I +VQA +T+L + S +P GGV++P AAF+ T
Sbjct: 350 GTDKNKPNIKICTQVKGPEAGYVATPIAMVQAAMTLLNDASHLPKAGGVFTPGAAFSKTK 409
Query: 380 YLERLQRNEINFAKISDTSV 439
++RL ++ I F+ IS + V
Sbjct: 410 LIDRLNKHGIEFSVISSSEV 429
>sp|Q8R127|SCPDH_MOUSE Probable saccharopine dehydrogenase
Length = 429
Score = 108 bits (270), Expect = 9e-24
Identities = 55/140 (39%), Positives = 83/140 (59%)
Frame = +2
Query: 20 MFIGGIFALFSNFGIGRTLLLKFPRFFSCGIVSFDGASRKAIENSKMELILIGRGWSNHS 199
MF G F F F IGR LL+KFP FS G S G ++K ++ + + G+G+S+ +
Sbjct: 290 MFAGLFFLFFVKFSIGRQLLIKFPWLFSFGYFSKQGPTQKQMDETSFTMTFFGQGYSHGT 349
Query: 200 SVDVEKPNRKIVCSISGPEPVYWSCSIFIVQAGLTILKEQSKMPGNGGVYSPLAAFNNTT 379
V+ KPN +I + GPE Y + I +VQA +T L + S +P GGV++P AAF+ T
Sbjct: 350 CVEKNKPNIRICTQVKGPEAGYVATPIAMVQAAMTFLSDASDLPKGGGVFTPGAAFSRTK 409
Query: 380 YLERLQRNEINFAKISDTSV 439
++RL ++ I F+ IS + V
Sbjct: 410 LIDRLNKHGIEFSVISSSEV 429
>sp|Q6AY30|SCPDH_RAT Probable saccharopine dehydrogenase
Length = 429
Score = 107 bits (266), Expect = 3e-23
Identities = 56/140 (40%), Positives = 82/140 (58%)
Frame = +2
Query: 20 MFIGGIFALFSNFGIGRTLLLKFPRFFSCGIVSFDGASRKAIENSKMELILIGRGWSNHS 199
MF G F F F IGR LL+KFP FS G S G ++K ++ S + G+G+S+
Sbjct: 290 MFAGLFFLFFVKFSIGRQLLVKFPWLFSFGYFSKRGPTQKQMDESSFTMTFFGQGYSHGV 349
Query: 200 SVDVEKPNRKIVCSISGPEPVYWSCSIFIVQAGLTILKEQSKMPGNGGVYSPLAAFNNTT 379
S + KPN +I + GPE Y + I +VQA +T L + S +P GGV++P AAF+ T
Sbjct: 350 SAEKNKPNIRICTQVKGPEAGYVATPIAMVQAAVTFLNDASDLPKGGGVFTPGAAFSRTK 409
Query: 380 YLERLQRNEINFAKISDTSV 439
++RL ++ I F+ IS + V
Sbjct: 410 LIDRLNQHGIQFSVISSSEV 429
>sp|Q8D2J7|SUFS_WIGBR Cysteine desulfurase (Selenocysteine lyase) (Selenocysteine
reductase) (Selenocysteine beta-lyase) (SCL)
Length = 410
Score = 30.8 bits (68), Expect = 2.4
Identities = 24/100 (24%), Positives = 42/100 (42%), Gaps = 2/100 (2%)
Frame = +2
Query: 134 RKAIENSKMELILIGRGWSNHSSVDVEKPNRKIVCS--ISGPEPVYWSCSIFIVQAGLTI 307
+K N +++++ G H +DV+K I C + +Y I ++ +
Sbjct: 188 KKIKNNYDIKVLIDGAQAIMHMKIDVQK----IDCDFYVFSGHKIYGPSGIGVLYGKKKL 243
Query: 308 LKEQSKMPGNGGVYSPLAAFNNTTYLERLQRNEINFAKIS 427
LK+ G GG+ + F+ TT+ E R E IS
Sbjct: 244 LKKMPPWEGGGGMIKKVCLFSGTTFNEIPWRFEAGSPNIS 283
>sp|Q9D666|UN84A_MOUSE Sad1/unc-84 protein-like 1 (Unc-84 homolog A)
Length = 913
Score = 29.6 bits (65), Expect = 5.4
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = -1
Query: 454 NIPLLNRCIRNLCKIYFISL 395
N+ LL RC+RN+CK++ + L
Sbjct: 402 NVFLLTRCLRNICKVFVLLL 421
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 65,964,973
Number of Sequences: 369166
Number of extensions: 1370416
Number of successful extensions: 3101
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 3023
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3101
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4078830820
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)