Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_024_B11
(414 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8CH02|SF04_MOUSE Splicing factor 4 152 2e-37
sp|Q68FU8|SF04_RAT Splicing factor 4 152 2e-37
sp|Q8IWZ8|SF04_HUMAN Splicing factor 4 (RNA-binding protein... 151 6e-37
sp|Q8CH09|SFR14_MOUSE Putative splicing factor, arginine/se... 36 0.035
sp|Q8IX01|SFR14_HUMAN Putative splicing factor, arginine/se... 36 0.035
sp|P46873|OSM3_CAEEL Osmotic avoidance abnormal protein 3 (... 34 0.10
sp|P14435|HA2F_MOUSE H-2 class II histocompatibility antige... 33 0.23
sp|Q6ND66|RIMM_RHOPA Probable 16S rRNA processing protein rimM 31 0.86
sp|Q03306|PKH3_YEAST Serine/threonine-protein kinase PKH3 (... 30 1.5
sp|Q14678|ANR15_HUMAN Ankyrin repeat domain protein 15 (Kid... 30 1.9
>sp|Q8CH02|SF04_MOUSE Splicing factor 4
Length = 643
Score = 152 bits (385), Expect = 2e-37
Identities = 74/138 (53%), Positives = 104/138 (75%), Gaps = 3/138 (2%)
Frame = +3
Query: 9 QKELQGMHEFITAQQKA-KEQELMNSIAGVTYGPAKTKLGQFKFEYDSDE--DAKEGTWE 179
Q+E+Q M++ I ++A ++ +L+ A L Q + YDSDE D++ GTWE
Sbjct: 446 QQEMQQMYDMIMQHKRAMQDMQLLWEKA----------LQQHQHGYDSDEEVDSELGTWE 495
Query: 180 HRLRKEEMEATRDWADKLTEMGQGKHHIGDFLPPEELDRFMETFRALKEGREPDYSDYTT 359
H+LR+ EM+ TR+WA++LT+MG+GKH IGDFLPP+EL++FMETF+ALKEGREPDYS+Y
Sbjct: 496 HQLRRMEMDKTREWAEQLTQMGRGKHFIGDFLPPDELEKFMETFKALKEGREPDYSEYKE 555
Query: 360 FKITCENVGFKMLKKMGW 413
FK+T EN+G++ML KMGW
Sbjct: 556 FKLTVENIGYQMLMKMGW 573
>sp|Q68FU8|SF04_RAT Splicing factor 4
Length = 644
Score = 152 bits (385), Expect = 2e-37
Identities = 74/138 (53%), Positives = 104/138 (75%), Gaps = 3/138 (2%)
Frame = +3
Query: 9 QKELQGMHEFITAQQKA-KEQELMNSIAGVTYGPAKTKLGQFKFEYDSDE--DAKEGTWE 179
Q+E+Q M++ I ++A ++ +L+ A L Q + YDSDE D++ GTWE
Sbjct: 447 QQEMQQMYDMIMQHKRAMQDMQLLWEKA----------LQQHQHGYDSDEEVDSELGTWE 496
Query: 180 HRLRKEEMEATRDWADKLTEMGQGKHHIGDFLPPEELDRFMETFRALKEGREPDYSDYTT 359
H+LR+ EM+ TR+WA++LT+MG+GKH IGDFLPP+EL++FMETF+ALKEGREPDYS+Y
Sbjct: 497 HQLRRMEMDKTREWAEQLTQMGRGKHFIGDFLPPDELEKFMETFKALKEGREPDYSEYKE 556
Query: 360 FKITCENVGFKMLKKMGW 413
FK+T EN+G++ML KMGW
Sbjct: 557 FKLTVENIGYQMLMKMGW 574
>sp|Q8IWZ8|SF04_HUMAN Splicing factor 4 (RNA-binding protein RBP)
Length = 645
Score = 151 bits (381), Expect = 6e-37
Identities = 73/138 (52%), Positives = 104/138 (75%), Gaps = 3/138 (2%)
Frame = +3
Query: 9 QKELQGMHEFITAQQKA-KEQELMNSIAGVTYGPAKTKLGQFKFEYDSDE--DAKEGTWE 179
Q+E+Q M++ I ++A ++ +L+ A + Q + YDSDE D++ GTWE
Sbjct: 448 QQEMQQMYDMIMQHKRAMQDMQLLWEKA----------VQQHQHGYDSDEEVDSELGTWE 497
Query: 180 HRLRKEEMEATRDWADKLTEMGQGKHHIGDFLPPEELDRFMETFRALKEGREPDYSDYTT 359
H+LR+ EM+ TR+WA++LT+MG+GKH IGDFLPP+EL++FMETF+ALKEGREPDYS+Y
Sbjct: 498 HQLRRMEMDKTREWAEQLTKMGRGKHFIGDFLPPDELEKFMETFKALKEGREPDYSEYKE 557
Query: 360 FKITCENVGFKMLKKMGW 413
FK+T EN+G++ML KMGW
Sbjct: 558 FKLTVENIGYQMLMKMGW 575
>sp|Q8CH09|SFR14_MOUSE Putative splicing factor, arginine/serine-rich 14
(Arginine/serine-rich splicing factor 14)
Length = 1067
Score = 35.8 bits (81), Expect = 0.035
Identities = 14/34 (41%), Positives = 25/34 (73%)
Frame = +3
Query: 312 RALKEGREPDYSDYTTFKITCENVGFKMLKKMGW 413
R + + ++P +++ K+T +NVGF+ML+KMGW
Sbjct: 976 RPIAKKKKPKDMEFSQQKLTDKNVGFQMLQKMGW 1009
>sp|Q8IX01|SFR14_HUMAN Putative splicing factor, arginine/serine-rich 14
(Arginine/serine-rich splicing factor 14)
Length = 1082
Score = 35.8 bits (81), Expect = 0.035
Identities = 14/34 (41%), Positives = 24/34 (70%)
Frame = +3
Query: 312 RALKEGREPDYSDYTTFKITCENVGFKMLKKMGW 413
R + + ++P D+ K+T +N+GF+ML+KMGW
Sbjct: 991 RPMSKKKKPKDLDFAQQKLTDKNLGFQMLQKMGW 1024
>sp|P46873|OSM3_CAEEL Osmotic avoidance abnormal protein 3 (Kinesin-like protein osm-3)
Length = 699
Score = 34.3 bits (77), Expect = 0.10
Identities = 26/93 (27%), Positives = 42/93 (45%), Gaps = 15/93 (16%)
Frame = +1
Query: 7 IRKSFKECMNSL-------QLNKKRRNKNL*IASQESHMDQRKQNWDNSNSNTIQMKMQK 165
+R+ F+E MN L Q +K K+L S + ++ N DN N K+Q+
Sbjct: 380 LREEFEEAMNDLRGEYEREQTSKAELQKDL--ESLRADYERANANLDNLNPEEAAKKIQQ 437
Query: 166 --------REPGSTDFVKKKWKQLETGQINSQK 240
E G+T +K+ KQL+ + +QK
Sbjct: 438 LQDQFIGGEEAGNTQLKQKRMKQLKEAETKTQK 470
>sp|P14435|HA2F_MOUSE H-2 class II histocompatibility antigen, A-F alpha chain
Length = 233
Score = 33.1 bits (74), Expect = 0.23
Identities = 21/65 (32%), Positives = 32/65 (49%), Gaps = 6/65 (9%)
Frame = +3
Query: 90 GVTYGPAKTKLGQFKFEYDSDE------DAKEGTWEHRLRKEEMEATRDWADKLTEMGQG 251
G++ + +GQ+ FE+D DE D KE W RL + + D L E+ G
Sbjct: 13 GISVYQSPGDIGQYTFEFDGDEWFYVDLDKKETVW--RLPEFGQLTSFDPQGGLQEIATG 70
Query: 252 KHHIG 266
KH++G
Sbjct: 71 KHNLG 75
>sp|Q6ND66|RIMM_RHOPA Probable 16S rRNA processing protein rimM
Length = 176
Score = 31.2 bits (69), Expect = 0.86
Identities = 24/67 (35%), Positives = 32/67 (47%)
Frame = +3
Query: 99 YGPAKTKLGQFKFEYDSDEDAKEGTWEHRLRKEEMEATRDWADKLTEMGQGKHHIGDFLP 278
YGP TK G +FE S +AK H + + TRD A++L G + D LP
Sbjct: 36 YGPLVTKDGARQFEIASAREAK----SHLVVTLKGVTTRDEAERLN--GVELYVARDKLP 89
Query: 279 PEELDRF 299
P E D +
Sbjct: 90 PTEADEY 96
>sp|Q03306|PKH3_YEAST Serine/threonine-protein kinase PKH3 (Pkb-activating kinase homolog
3)
Length = 898
Score = 30.4 bits (67), Expect = 1.5
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = -3
Query: 160 ASSSESYSNLNCPNFVFAGPYVTPAMLFISSCSF 59
+ S+ S N NC +FV YV+P +L + C +
Sbjct: 176 SDSNGSKDNQNCASFVGTAEYVSPELLLYNQCGY 209
>sp|Q14678|ANR15_HUMAN Ankyrin repeat domain protein 15 (Kidney ankyrin repeat-containing
protein)
Length = 1352
Score = 30.0 bits (66), Expect = 1.9
Identities = 26/71 (36%), Positives = 32/71 (45%)
Frame = -3
Query: 304 SIKRSNSSGGRKSPMWCFPCPISVSLSAQSRVASISSLRSLCSQVPSFASSSESYSNLNC 125
+IKR N RK + PCP + S Q + + S SL SSS S N C
Sbjct: 57 TIKRLNIQKRRKPSV---PCPEPRTTSGQQGIWT--STESL--------SSSNSDDNKQC 103
Query: 124 PNFVFAGPYVT 92
PNF+ A VT
Sbjct: 104 PNFLIARSQVT 114
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 49,393,198
Number of Sequences: 369166
Number of extensions: 944925
Number of successful extensions: 3392
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3275
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3386
length of database: 68,354,980
effective HSP length: 99
effective length of database: 50,066,215
effective search space used: 1902516170
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)