Planarian EST Database


Dr_sW_024_B03

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_024_B03
         (712 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q91YX5|LGAT1_MOUSE  Acyl-CoA:lysophosphatidylglycerol acy...   124   3e-28
sp|Q92604|LGAT1_HUMAN  Acyl-CoA:lysophosphatidylglycerol acy...   120   5e-27
sp|Q11087|YT73_CAEEL  Hypothetical protein C01C10.3 in chrom...    69   9e-12
sp|P38226|YB42_YEAST  Hypothetical 45.5 kDa protein in FAT1-...    37   0.068
sp|Q6IWY1|LPAT2_BRAOL  1-acyl-sn-glycerol-3-phosphate acyltr...    32   2.2  
sp|Q08235|BRX1_YEAST  Ribosome biogenesis protein BRX1             32   2.2  
sp|P24870|CG23_YEAST  G2/mitotic-specific cyclin-3                 30   4.9  
>sp|Q91YX5|LGAT1_MOUSE Acyl-CoA:lysophosphatidylglycerol acyltransferase 1
          Length = 370

 Score =  124 bits (310), Expect = 3e-28
 Identities = 56/121 (46%), Positives = 83/121 (68%)
 Frame = +2

Query: 2   NHQSTVDVPLIMHLLNFRKYLIFHSSWMLDEMFKYAPFGWVSLIRNDIFVKQGRETRNQQ 181
           NHQ+T DV  +M  L  +  ++    W++D +FKY  FG VSLI  D F++QGR  R+QQ
Sbjct: 100 NHQATGDVCTLMMCLQDKGPVVAQMMWLMDHIFKYTNFGIVSLIHGDFFIRQGRAYRDQQ 159

Query: 182 LSILPEKFKYSFSKLDRKWLIIFPEGGFFYKRKESSKEYALKNNYPKLDYVTLPRMGAVD 361
           L +L +  ++++   DRKW+++FPEGGF  KR+E+S+ +A KNN P L +VTLPR GA +
Sbjct: 160 LLVLKKHLEHNYRSRDRKWIVLFPEGGFLRKRRETSQAFAKKNNLPFLTHVTLPRFGATN 219

Query: 362 V 364
           +
Sbjct: 220 I 220

 Score = 50.4 bits (119), Expect = 5e-06
 Identities = 26/69 (37%), Positives = 42/69 (60%)
 Frame = +1

Query: 505 IKYIIDITIAYNNIECPPSFVSMVLGSHSTCPVYVYYRVYNIGQIPVASSYELQSWMYKI 684
           +++IID TIAY   E P    + +LG       +V+YR++ IG +P+ +  +L SW+Y+ 
Sbjct: 249 LQWIIDTTIAYPKAE-PIDIQTWILGYRKPTVTHVHYRIFPIGDVPLETE-DLTSWLYQR 306

Query: 685 YKEKNDRLS 711
           + EK D LS
Sbjct: 307 FIEKEDLLS 315
>sp|Q92604|LGAT1_HUMAN Acyl-CoA:lysophosphatidylglycerol acyltransferase 1
          Length = 370

 Score =  120 bits (300), Expect = 5e-27
 Identities = 55/121 (45%), Positives = 80/121 (66%)
 Frame = +2

Query: 2   NHQSTVDVPLIMHLLNFRKYLIFHSSWMLDEMFKYAPFGWVSLIRNDIFVKQGRETRNQQ 181
           NHQ+T DV  +M  L  +  ++    W++D +FKY  FG VSL+  D F++QGR  R+QQ
Sbjct: 100 NHQATGDVCTLMMCLQDKGLVVAQMMWLMDHIFKYTNFGIVSLVHGDFFIRQGRSYRDQQ 159

Query: 182 LSILPEKFKYSFSKLDRKWLIIFPEGGFFYKRKESSKEYALKNNYPKLDYVTLPRMGAVD 361
           L +L +  + ++   DRKW+++FPEGGF  KR+E+S+ +A KNN P L  VTLPR GA  
Sbjct: 160 LLLLKKHLENNYRSRDRKWIVLFPEGGFLRKRRETSQAFAKKNNLPFLTNVTLPRSGATK 219

Query: 362 V 364
           +
Sbjct: 220 I 220

 Score = 49.3 bits (116), Expect = 1e-05
 Identities = 28/77 (36%), Positives = 46/77 (59%), Gaps = 1/77 (1%)
 Frame = +1

Query: 484 ELSAKHK-IKYIIDITIAYNNIECPPSFVSMVLGSHSTCPVYVYYRVYNIGQIPVASSYE 660
           EL +K K +++IID TIAY   E P    + +LG       +V+YR++ I  +P+ +  +
Sbjct: 241 ELDSKSKGLQWIIDTTIAYPKAE-PIDIQTWILGYRKPTVTHVHYRIFPIKDVPLETD-D 298

Query: 661 LQSWMYKIYKEKNDRLS 711
           L +W+Y+ + EK D LS
Sbjct: 299 LTTWLYQRFVEKEDLLS 315
>sp|Q11087|YT73_CAEEL Hypothetical protein C01C10.3 in chromosome X
          Length = 391

 Score = 69.3 bits (168), Expect = 9e-12
 Identities = 39/118 (33%), Positives = 57/118 (48%)
 Frame = +2

Query: 2   NHQSTVDVPLIMHLLNFRKYLIFHSSWMLDEMFKYAPFGWVSLIRNDIFVKQGRETRNQQ 181
           NH   +D  ++M  LN +  +     W++  ++KY P G +     + FV  G   R+  
Sbjct: 123 NHLGLLDHFVLMQSLNGKGSIRSRWMWVIYNIWKYTPLGVMWTSHGNFFVNGGVSKRDSV 182

Query: 182 LSILPEKFKYSFSKLDRKWLIIFPEGGFFYKRKESSKEYALKNNYPKLDYVTLPRMGA 355
           LS   +  K SF K D  W+I++PEG   Y  K S + +A KN    LD    PR GA
Sbjct: 183 LSSFRDHLKNSFYKYDYGWVIMYPEGSRLYLVKNSGRTFAEKNGLKPLDNCVYPRTGA 240
>sp|P38226|YB42_YEAST Hypothetical 45.5 kDa protein in FAT1-TCM62 intergenic region
          Length = 397

 Score = 36.6 bits (83), Expect = 0.068
 Identities = 21/74 (28%), Positives = 35/74 (47%), Gaps = 6/74 (8%)
 Frame = +1

Query: 496 KHKIKYIIDITIAYNNI------ECPPSFVSMVLGSHSTCPVYVYYRVYNIGQIPVASSY 657
           K  I+ + DITI Y+ +      E      S+ L       V ++ R +++  IP+    
Sbjct: 268 KPSIESLYDITIGYSGVKQEEYGELIYGLKSIFLEGKYPKLVDIHIRAFDVKDIPLEDEN 327

Query: 658 ELQSWMYKIYKEKN 699
           E   W+YKI+ EK+
Sbjct: 328 EFSEWLYKIWSEKD 341
>sp|Q6IWY1|LPAT2_BRAOL 1-acyl-sn-glycerol-3-phosphate acyltransferase 2 (Lysophosphatidyl
           acyltransferase 2)
          Length = 391

 Score = 31.6 bits (70), Expect = 2.2
 Identities = 31/128 (24%), Positives = 55/128 (42%), Gaps = 1/128 (0%)
 Frame = +2

Query: 8   QSTVDVPLIMHLLNFRKYLIFHSSWMLDEMFKYAPFGWVSLIRNDIFVKQGRETRNQQLS 187
           +S+  +P+I   + F +YL    +W  DE          S +++ +          Q+L+
Sbjct: 120 KSSKFLPVIGWSMWFSEYLFLERNWAKDE----------STLKSGL----------QRLN 159

Query: 188 ILPEKFKYSFSKLDRKWLIIFPEGGFFYKRK-ESSKEYALKNNYPKLDYVTLPRMGAVDV 364
             P  F          WL +F EG  F + K ++++EYA  +  P    V +PR      
Sbjct: 160 DFPRPF----------WLALFVEGTRFTEAKLKAAQEYAATSQLPVPRNVLIPRTKGFVS 209

Query: 365 TV*NVKSY 388
            V N++S+
Sbjct: 210 AVSNMRSF 217
>sp|Q08235|BRX1_YEAST Ribosome biogenesis protein BRX1
          Length = 291

 Score = 31.6 bits (70), Expect = 2.2
 Identities = 21/53 (39%), Positives = 29/53 (54%), Gaps = 1/53 (1%)
 Frame = -2

Query: 312 LFFKAYSLLDSFLL*KNPPSGKIINHFLSNLEKL-YLNFSGSIDNC*FRVSRP 157
           LFF+A    D +L    PP+G  I  ++ NL  +  LNF+G   NC  + SRP
Sbjct: 87  LFFEARKHQDLYLWLSKPPNGPTIKFYIQNLHTMDELNFTG---NC-LKGSRP 135
>sp|P24870|CG23_YEAST G2/mitotic-specific cyclin-3
          Length = 427

 Score = 30.4 bits (67), Expect = 4.9
 Identities = 20/81 (24%), Positives = 39/81 (48%), Gaps = 9/81 (11%)
 Frame = +1

Query: 463 VNKKSDYELSAKHKIKYIIDITIA-YNNIECPPSFVS--------MVLGSHSTCPVYVYY 615
           ++K  DYE   +   KY+++ TI  +  +   PS+++        ++LG +     +VYY
Sbjct: 308 ISKADDYEHDTRTLAKYLLESTIMDHRLVSAQPSWLAAGAYFLSKIILGQNQWSLAHVYY 367

Query: 616 RVYNIGQIPVASSYELQSWMY 678
             Y   QI   ++  L++  Y
Sbjct: 368 SNYTQEQILPLATIILENCRY 388
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 77,247,445
Number of Sequences: 369166
Number of extensions: 1480957
Number of successful extensions: 3668
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 3567
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3667
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6267895215
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)