Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_024_A23
(690 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q13043|STK4_HUMAN Serine/threonine-protein kinase 4 (STE... 91 3e-18
sp|Q9SZ67|WRK19_ARATH Probable WRKY transcription factor 19... 90 6e-18
sp|Q9JI10|STK3_MOUSE Serine/threonine-protein kinase 3 (STE... 89 8e-18
sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinas... 89 8e-18
sp|Q13188|STK3_HUMAN Serine/threonine-protein kinase 3 (STE... 88 2e-17
sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinas... 88 2e-17
sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinas... 88 2e-17
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 87 4e-17
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 87 5e-17
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 86 7e-17
>sp|Q13043|STK4_HUMAN Serine/threonine-protein kinase 4 (STE20-like kinase MST1) (MST-1)
(Mammalian STE20-like protein kinase 1)
(Serine/threonine-protein kinase Krs-2)
Length = 487
Score = 90.9 bits (224), Expect = 3e-18
Identities = 55/189 (29%), Positives = 94/189 (49%), Gaps = 1/189 (0%)
Frame = +2
Query: 23 DTSRFVQLLSMYEEENSVFIVMERM-ASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYM 199
D+ V+ Y + ++IVME A S+ +++ + LTED+I L GL Y+
Sbjct: 81 DSPHVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLTEDEIATILQSTLKGLEYL 140
Query: 200 HKLKILHRSLNCGSVLIDCNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAP 379
H ++ +HR + G++L++ +AKL +F ++ L D K + + W+AP
Sbjct: 141 HFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMA-------KRNTVIGTPFWMAP 193
Query: 380 EVLLGEPYGFKADIWSFGVTMLEMMTGRRPYPQLNNVQAAHRIVADRHIEFDRDLYPNSG 559
EV+ Y ADIWS G+T +EM G+ PY ++ ++A I + F + +
Sbjct: 194 EVIQEIGYNCVADIWSLGITAIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDN 253
Query: 560 FDDILDTCL 586
F D + CL
Sbjct: 254 FTDFVKQCL 262
>sp|Q9SZ67|WRK19_ARATH Probable WRKY transcription factor 19 (WRKY DNA-binding protein 19)
Length = 1895
Score = 89.7 bits (221), Expect = 6e-18
Identities = 62/184 (33%), Positives = 96/184 (52%), Gaps = 5/184 (2%)
Frame = +2
Query: 59 EEENSVFIVMERMAS-SLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKILHRSLNC 235
++E++++I +E + SLR L + + L + + Y+ +ILDGL Y+H +HR++ C
Sbjct: 1695 KDESNLYIFLELVTQGSLRKLYQRN--QLGDSVVSLYTRQILDGLKYLHDKGFIHRNIKC 1752
Query: 236 GSVLIDCNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAPEVLLG----EPY 403
+VL+D N KL +F L+K + T W+APEV+L + Y
Sbjct: 1753 ANVLVDANGTVKLADFGLAKVMSLWRTPYW-------------NWMAPEVILNPKDYDGY 1799
Query: 404 GFKADIWSFGVTMLEMMTGRRPYPQLNNVQAAHRIVADRHIEFDRDLYPNSGFDDILDTC 583
G ADIWS G T+LEM+TG+ PY L A + I + + D+ D IL TC
Sbjct: 1800 GTPADIWSLGCTVLEMLTGQIPYSDLEIGTALYNIGTGKLPKIP-DILSLDARDFIL-TC 1857
Query: 584 LRSN 595
L+ N
Sbjct: 1858 LKVN 1861
>sp|Q9JI10|STK3_MOUSE Serine/threonine-protein kinase 3 (STE20-like kinase MST2) (MST-2)
(Mammalian STE20-like protein kinase 2)
Length = 497
Score = 89.4 bits (220), Expect = 8e-18
Identities = 55/189 (29%), Positives = 94/189 (49%), Gaps = 1/189 (0%)
Frame = +2
Query: 23 DTSRFVQLLSMYEEENSVFIVMERM-ASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYM 199
D+ V+ Y + ++IVME A S+ +++ + LTED+I L GL Y+
Sbjct: 78 DSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLTEDEIATILKSTLKGLEYL 137
Query: 200 HKLKILHRSLNCGSVLIDCNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAP 379
H ++ +HR + G++L++ +AKL +F ++ L D K + + W+AP
Sbjct: 138 HFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMA-------KRNTVIGTPFWMAP 190
Query: 380 EVLLGEPYGFKADIWSFGVTMLEMMTGRRPYPQLNNVQAAHRIVADRHIEFDRDLYPNSG 559
EV+ Y ADIWS G+T +EM G+ PY ++ ++A I + F + +
Sbjct: 191 EVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDD 250
Query: 560 FDDILDTCL 586
F D + CL
Sbjct: 251 FTDFVKKCL 259
>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 89.4 bits (220), Expect = 8e-18
Identities = 55/150 (36%), Positives = 86/150 (57%), Gaps = 2/150 (1%)
Frame = +2
Query: 65 ENSVFIVMERM-ASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKILHRSLNCGS 241
E ++ I ME M S++ LK LTE +KY+ +IL+G+SY+H I+HR + +
Sbjct: 436 EKTLTIFMEYMPGGSVKDQLKAYGA-LTESVTRKYTRQILEGMSYLHSNMIVHRDIKGAN 494
Query: 242 VLIDCNDNAKLCNFHLSKFLFD-CSTEQLLKGVKGSKIEDDECWVAPEVLLGEPYGFKAD 418
+L D N KL +F SK L C + ++ V G+ W++PEV+ GE YG KAD
Sbjct: 495 ILRDSAGNVKLGDFGASKRLQTICMSGTGMRSVTGTPY-----WMSPEVISGEGYGRKAD 549
Query: 419 IWSFGVTMLEMMTGRRPYPQLNNVQAAHRI 508
+WS G T++EM+T + P+ + + A +I
Sbjct: 550 VWSLGCTVVEMLTEKPPWAEYEAMAAIFKI 579
>sp|Q13188|STK3_HUMAN Serine/threonine-protein kinase 3 (STE20-like kinase MST2) (MST-2)
(Mammalian STE20-like protein kinase 2)
(Serine/threonine-protein kinase Krs-1)
Length = 491
Score = 88.2 bits (217), Expect = 2e-17
Identities = 55/192 (28%), Positives = 94/192 (48%), Gaps = 1/192 (0%)
Frame = +2
Query: 23 DTSRFVQLLSMYEEENSVFIVMERM-ASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYM 199
D+ V+ Y + ++IVME A S+ +++ + L ED+I L GL Y+
Sbjct: 78 DSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYL 137
Query: 200 HKLKILHRSLNCGSVLIDCNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAP 379
H ++ +HR + G++L++ +AKL +F ++ L D K + + W+AP
Sbjct: 138 HFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMA-------KRNTVIGTPFWMAP 190
Query: 380 EVLLGEPYGFKADIWSFGVTMLEMMTGRRPYPQLNNVQAAHRIVADRHIEFDRDLYPNSG 559
EV+ Y ADIWS G+T +EM G+ PY ++ ++A I + F + +
Sbjct: 191 EVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDD 250
Query: 560 FDDILDTCLRSN 595
F D + CL N
Sbjct: 251 FTDFVKKCLVKN 262
>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 88.2 bits (217), Expect = 2e-17
Identities = 55/150 (36%), Positives = 85/150 (56%), Gaps = 2/150 (1%)
Frame = +2
Query: 65 ENSVFIVMERM-ASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKILHRSLNCGS 241
E + I ME M S++ LK LTE +KY+ +IL+G+SY+H I+HR + +
Sbjct: 436 EKILTIFMEYMPGGSVKDQLKAYGA-LTESVTRKYTRQILEGMSYLHSNMIVHRDIKGAN 494
Query: 242 VLIDCNDNAKLCNFHLSKFLFD-CSTEQLLKGVKGSKIEDDECWVAPEVLLGEPYGFKAD 418
+L D N KL +F SK L C + ++ V G+ W++PEV+ GE YG KAD
Sbjct: 495 ILRDSAGNVKLGDFGASKRLQTICMSGTGIRSVTGTPY-----WMSPEVISGEGYGRKAD 549
Query: 419 IWSFGVTMLEMMTGRRPYPQLNNVQAAHRI 508
+WS G T++EM+T + P+ + + A +I
Sbjct: 550 VWSLGCTVVEMLTEKPPWAEYEAMAAIFKI 579
>sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 618
Score = 88.2 bits (217), Expect = 2e-17
Identities = 55/151 (36%), Positives = 86/151 (56%), Gaps = 2/151 (1%)
Frame = +2
Query: 62 EENSVFIVMERM-ASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKILHRSLNCG 238
+E ++ I ME M S++ LK LTE+ +KY+ +IL+G+ Y+H ILHR +
Sbjct: 428 QEKTLSIFMEYMPGGSIKDQLKAYGA-LTENGTRKYTRQILEGVHYLHSNMILHRDIKGA 486
Query: 239 SVLIDCNDNAKLCNFHLSKFLFD-CSTEQLLKGVKGSKIEDDECWVAPEVLLGEPYGFKA 415
++L D N KL +F SK L C + +K V G+ W++PEV+ G+ YG KA
Sbjct: 487 NILRDSTGNVKLGDFGASKRLQTICLSGTGMKSVTGTPY-----WMSPEVISGQGYGRKA 541
Query: 416 DIWSFGVTMLEMMTGRRPYPQLNNVQAAHRI 508
DIWS T++EM+T + P+ + + A +I
Sbjct: 542 DIWSVACTVVEMLTEKPPWAEFEAMAAIFKI 572
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 87.0 bits (214), Expect = 4e-17
Identities = 48/147 (32%), Positives = 81/147 (55%)
Frame = +2
Query: 38 VQLLSMYEEENSVFIVMERMASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKIL 217
V+ L E++++ I++E + S L E ++ Y+ ++L GL Y+H I+
Sbjct: 135 VRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYTRQLLLGLEYLHNHAIM 194
Query: 218 HRSLNCGSVLIDCNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAPEVLLGE 397
HR + ++L+D KL +F SK + + +T K +KG+ W+APEV+L
Sbjct: 195 HRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTPY-----WMAPEVILQT 249
Query: 398 PYGFKADIWSFGVTMLEMMTGRRPYPQ 478
+ F ADIWS G T++EM+TG+ P+ Q
Sbjct: 250 GHSFSADIWSVGCTVIEMVTGKAPWSQ 276
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 86.7 bits (213), Expect = 5e-17
Identities = 48/147 (32%), Positives = 80/147 (54%)
Frame = +2
Query: 38 VQLLSMYEEENSVFIVMERMASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKIL 217
V+ L E+ ++ I++E + S L E ++ Y+ ++L GL Y+H I+
Sbjct: 134 VRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRTYTNQLLLGLEYLHNHAIM 193
Query: 218 HRSLNCGSVLIDCNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAPEVLLGE 397
HR + ++L+D KL +F SK + + +T K +KG+ W+APEV+L
Sbjct: 194 HRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKGTPY-----WMAPEVILQT 248
Query: 398 PYGFKADIWSFGVTMLEMMTGRRPYPQ 478
+ F ADIWS G T++EM+TG+ P+ Q
Sbjct: 249 GHSFSADIWSVGCTVIEMVTGKAPWSQ 275
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 86.3 bits (212), Expect = 7e-17
Identities = 60/185 (32%), Positives = 90/185 (48%), Gaps = 2/185 (1%)
Frame = +2
Query: 38 VQLLSMYEEENSVFIVMERMASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKIL 217
V+ L E +S+ I+ME + S L E I Y+ ++L GL Y+H I+
Sbjct: 134 VRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYTKQLLLGLEYLHNNGIM 193
Query: 218 HRSLNCGSVLIDCNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAPEVLLGE 397
HR + ++L+D +L +F SK + + +T K +KG+ W+APEV+L
Sbjct: 194 HRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTPY-----WMAPEVILQT 248
Query: 398 PYGFKADIWSFGVTMLEMMTGRRPYPQLNNVQAA--HRIVADRHIEFDRDLYPNSGFDDI 571
+ F ADIWS G T++EM TG+ P+ + AA H H DL P + D
Sbjct: 249 GHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKAHPPIPEDLSPEA--KDF 306
Query: 572 LDTCL 586
L CL
Sbjct: 307 LMKCL 311
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 82,183,749
Number of Sequences: 369166
Number of extensions: 1730610
Number of successful extensions: 7837
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6497
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6801
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5927776870
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)