Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_024_A06
(844 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P26368|U2AF2_HUMAN Splicing factor U2AF 65 kDa subunit (... 301 2e-81
sp|P26369|U2AF2_MOUSE Splicing factor U2AF 65 kDa subunit (... 301 2e-81
sp|Q24562|U2AF2_DROME Splicing factor U2AF 50 kDa subunit (... 294 2e-79
sp|P90978|U2AF2_CAEEL Splicing factor U2AF 65 kDa subunit (... 241 2e-63
sp|P90727|U2AF2_CAEBR Splicing factor U2AF 65 kDa subunit (... 238 1e-62
sp|P36629|U2AF2_SCHPO Splicing factor U2AF 59 kDa subunit (... 96 1e-19
sp|Q86U06|RBM23_HUMAN Probable RNA-binding protein 23 (RNA-... 62 3e-09
sp|O43040|CTF1_SCHPO Cleavage and termination factor 1 (Tra... 61 4e-09
sp|Q8BIQ5|CSTF2_MOUSE Cleavage stimulation factor, 64 kDa s... 60 8e-09
sp|P33240|CSTF2_HUMAN Cleavage stimulation factor, 64 kDa s... 60 8e-09
>sp|P26368|U2AF2_HUMAN Splicing factor U2AF 65 kDa subunit (U2 auxiliary factor 65 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
(hU2AF(65))
Length = 475
Score = 301 bits (770), Expect = 2e-81
Identities = 159/292 (54%), Positives = 204/292 (69%), Gaps = 11/292 (3%)
Frame = +1
Query: 1 GYEHMSPAEFKAMQAAGQV-----LPTIYTTEGGVVNTEQVLDQHPSIGSAVSRQARRIY 165
G+EH++P ++KAMQAAGQ+ LPT+ T +G V V P +GS ++RQARR+Y
Sbjct: 98 GFEHITPMQYKAMQAAGQIPATALLPTM-TPDGLAVTPTPV----PVVGSQMTRQARRLY 152
Query: 166 VGNIPLGVTEQGMMDFFNEQMKLKLFTQAEGDPIIAVQINTDKNFAFLEFRSVEETSQGL 345
VGNIP G+TE+ MMDFFN QM+L TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +
Sbjct: 153 VGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAM 212
Query: 346 NLDGIMYNGFPLKLRRPRDYVPLPTTMD--TMLSNSLAAGIVQDTPHKLFIGGLPHYLNE 519
DGI++ G LK+RRP DY PLP + ++ + + +V D+ HKLFIGGLP+YLN+
Sbjct: 213 AFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLND 272
Query: 520 DQVKELLLTFGPLSGFNLVKDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVV 699
DQVKELL +FGPL FNLVKDS+TGLSKGYAFCEY D +VTD+A GL GMQLG+K+++V
Sbjct: 273 DQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLV 332
Query: 700 QRASLGAKNPXXXXXXXXXXXXXXXXG----MDPSIGTCGQETEILCLHNMI 843
QRAS+GAKN M + G TE+LCL NM+
Sbjct: 333 QRASVGAKNATLVSPPSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMV 384
>sp|P26369|U2AF2_MOUSE Splicing factor U2AF 65 kDa subunit (U2 auxiliary factor 65 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
Length = 475
Score = 301 bits (770), Expect = 2e-81
Identities = 159/292 (54%), Positives = 204/292 (69%), Gaps = 11/292 (3%)
Frame = +1
Query: 1 GYEHMSPAEFKAMQAAGQV-----LPTIYTTEGGVVNTEQVLDQHPSIGSAVSRQARRIY 165
G+EH++P ++KAMQAAGQ+ LPT+ T +G V V P +GS ++RQARR+Y
Sbjct: 98 GFEHITPMQYKAMQAAGQIPATALLPTM-TPDGLAVTPTPV----PVVGSQMTRQARRLY 152
Query: 166 VGNIPLGVTEQGMMDFFNEQMKLKLFTQAEGDPIIAVQINTDKNFAFLEFRSVEETSQGL 345
VGNIP G+TE+ MMDFFN QM+L TQA G+P++AVQIN DKNFAFLEFRSV+ET+Q +
Sbjct: 153 VGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAM 212
Query: 346 NLDGIMYNGFPLKLRRPRDYVPLPTTMD--TMLSNSLAAGIVQDTPHKLFIGGLPHYLNE 519
DGI++ G LK+RRP DY PLP + ++ + + +V D+ HKLFIGGLP+YLN+
Sbjct: 213 AFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLND 272
Query: 520 DQVKELLLTFGPLSGFNLVKDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVV 699
DQVKELL +FGPL FNLVKDS+TGLSKGYAFCEY D +VTD+A GL GMQLG+K+++V
Sbjct: 273 DQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLV 332
Query: 700 QRASLGAKNPXXXXXXXXXXXXXXXXG----MDPSIGTCGQETEILCLHNMI 843
QRAS+GAKN M + G TE+LCL NM+
Sbjct: 333 QRASVGAKNATLVSLPSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMV 384
>sp|Q24562|U2AF2_DROME Splicing factor U2AF 50 kDa subunit (U2 auxiliary factor 50 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
Length = 416
Score = 294 bits (753), Expect = 2e-79
Identities = 152/287 (52%), Positives = 203/287 (70%), Gaps = 6/287 (2%)
Frame = +1
Query: 1 GYEHMSPAEFKAMQAAGQVLPTIYTTEGGVVNTEQVLDQHPSIGSAVSRQARRIYVGNIP 180
G+EH++P ++KAMQA+GQ+ ++ V +T Q P +GS ++RQARR+YVGNIP
Sbjct: 50 GFEHITPMQYKAMQASGQIPASV------VPDTPQTAV--PVVGSTITRQARRLYVGNIP 101
Query: 181 LGVTEQGMMDFFNEQMKLKLFTQAEGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGI 360
GVTE+ MM+FFN+QM L QA G P++A QIN DKNFAFLEFRS++ET+Q + DGI
Sbjct: 102 FGVTEEEMMEFFNQQMHLVGLAQAAGSPVLACQINLDKNFAFLEFRSIDETTQAMAFDGI 161
Query: 361 MYNGFPLKLRRPRDYVPLPTTMDT------MLSNSLAAGIVQDTPHKLFIGGLPHYLNED 522
G LK+RRP DY P+P DT ++S+ + + +V D+PHK+FIGGLP+YLN+D
Sbjct: 162 NLKGQSLKIRRPHDYQPMPGITDTPAIKPAVVSSGVISTVVPDSPHKIFIGGLPNYLNDD 221
Query: 523 QVKELLLTFGPLSGFNLVKDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQ 702
QVKELLL+FG L FNLVKD++TGLSKGYAFCEY D S+TD++ GL GMQLG+K+++VQ
Sbjct: 222 QVKELLLSFGKLRAFNLVKDAATGLSKGYAFCEYVDLSITDQSIAGLNGMQLGDKKLIVQ 281
Query: 703 RASLGAKNPXXXXXXXXXXXXXXXXGMDPSIGTCGQETEILCLHNMI 843
RAS+GAKN G+ ++ T G TE+LCL NM+
Sbjct: 282 RASVGAKN-AQNAANTTQSVMLQVPGLS-NVVTSGPPTEVLCLLNMV 326
>sp|P90978|U2AF2_CAEEL Splicing factor U2AF 65 kDa subunit (U2 auxiliary factor 65 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
(U2AF65)
Length = 496
Score = 241 bits (614), Expect = 2e-63
Identities = 140/281 (49%), Positives = 178/281 (63%)
Frame = +1
Query: 1 GYEHMSPAEFKAMQAAGQVLPTIYTTEGGVVNTEQVLDQHPSIGSAVSRQARRIYVGNIP 180
G+E +P E+K MQAAGQV G V + P +G +V+ Q+RR+YVGNIP
Sbjct: 145 GFETTTPMEYKNMQAAGQV------PRGSVQSAV------PVVGPSVTCQSRRLYVGNIP 192
Query: 181 LGVTEQGMMDFFNEQMKLKLFTQAEGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGI 360
G E+ M+DFFN+QM L QA G+PI+ QIN DKNFAF+EFRS++ET+ G+ DGI
Sbjct: 193 FGCNEEAMLDFFNQQMHLCGLAQAPGNPILLCQINLDKNFAFIEFRSIDETTAGMAFDGI 252
Query: 361 MYNGFPLKLRRPRDYVPLPTTMDTMLSNSLAAGIVQDTPHKLFIGGLPHYLNEDQVKELL 540
+ G LK+RRPRDY P T D M S + IV D+ +K+FIGGLP+YL EDQVKELL
Sbjct: 253 NFMGQQLKVRRPRDYQPSQNTFD-MNSRMPVSTIVVDSANKIFIGGLPNYLTEDQVKELL 311
Query: 541 LTFGPLSGFNLVKDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGA 720
+FGPL F+L DS G SKGYAF EY D ++TD+A GL GMQLG+K++VVQ A
Sbjct: 312 CSFGPLKAFSLNVDSQ-GNSKGYAFAEYLDPTLTDQAIAGLNGMQLGDKQLVVQLACANQ 370
Query: 721 KNPXXXXXXXXXXXXXXXXGMDPSIGTCGQETEILCLHNMI 843
+ G+D S G G+ TEILCL NM+
Sbjct: 371 QR----HNTNLPNSASAIAGIDLSQG-AGRATEILCLMNMV 406
>sp|P90727|U2AF2_CAEBR Splicing factor U2AF 65 kDa subunit (U2 auxiliary factor 65 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
(U2AF65)
Length = 488
Score = 238 bits (608), Expect = 1e-62
Identities = 137/281 (48%), Positives = 178/281 (63%)
Frame = +1
Query: 1 GYEHMSPAEFKAMQAAGQVLPTIYTTEGGVVNTEQVLDQHPSIGSAVSRQARRIYVGNIP 180
G+E+++P E+K MQA+G V G V + P +G +V+ Q+RR+YVGNIP
Sbjct: 136 GFENITPMEYKNMQASGAV------PRGSVQSAV------PVVGPSVTCQSRRLYVGNIP 183
Query: 181 LGVTEQGMMDFFNEQMKLKLFTQAEGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGI 360
G E+ M+DFFN+QM L QA G+PI+ QIN DKNFAF+EFRS++ET+ G+ DGI
Sbjct: 184 FGCNEEAMLDFFNQQMHLCNLAQAPGNPILLCQINLDKNFAFIEFRSIDETTAGMAFDGI 243
Query: 361 MYNGFPLKLRRPRDYVPLPTTMDTMLSNSLAAGIVQDTPHKLFIGGLPHYLNEDQVKELL 540
+ G LK+RRPRDY P T D M + + IV D+ +K+FIGGLP+YL EDQVKELL
Sbjct: 244 NFMGQQLKVRRPRDYQPSQNTFD-MNARMPVSSIVVDSANKIFIGGLPNYLTEDQVKELL 302
Query: 541 LTFGPLSGFNLVKDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGA 720
+FGPL F+L DS G SKGYAF EY D ++TD+A GL GMQLG+K++VVQ A
Sbjct: 303 CSFGPLKAFSLNVDSQ-GNSKGYAFAEYLDPTLTDQAIAGLNGMQLGDKQLVVQLACANQ 361
Query: 721 KNPXXXXXXXXXXXXXXXXGMDPSIGTCGQETEILCLHNMI 843
G+D S G G+ TEILCL NM+
Sbjct: 362 TR----HNTHLPNSASAIAGIDLSQG-AGRATEILCLMNMV 397
>sp|P36629|U2AF2_SCHPO Splicing factor U2AF 59 kDa subunit (U2 auxiliary factor 59 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
(U2AF59)
Length = 517
Score = 95.9 bits (237), Expect = 1e-19
Identities = 73/244 (29%), Positives = 107/244 (43%), Gaps = 3/244 (1%)
Frame = +1
Query: 121 PSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQAEGDPIIAVQINTDKNF 300
P + SRQARR+ V IP E + F + + + E +V + ++NF
Sbjct: 188 PPLQPGASRQARRLVVTGIPNEFVEDAFVSFIEDLFISTTYHKPETKHFSSVNVCKEENF 247
Query: 301 AFLEFRSVEETSQGLNLDGIMY-NGFPLKLRRPRDYVPLPTTMDTML--SNSLAAGIVQD 471
A LE + E+ + L Y N LK +R ++Y+ T + S+ A V D
Sbjct: 248 AILEVATPEDATFLWGLQSESYSNDVFLKFQRIQNYIVPQITPEVSQKRSDDYAKNDVLD 307
Query: 472 TPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVKDSSTGLSKGYAFCEYADHSVTDKA 651
+ K++I LP L EDQV ELL FG L F L+K+ + G SKG+ FCE+ + S + A
Sbjct: 308 SKDKIYISNLPLNLGEDQVVELLKPFGDLLSFQLIKNIADGSSKGFCFCEFKNPSDAEVA 367
Query: 652 CLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSIGTCGQETEILCL 831
GL G ++ Q A +G SI T +L L
Sbjct: 368 ISGLDGKDTYGNKLHAQFACVGLNQAMIDKSNGMAILTELAKASSQSI-----PTRVLQL 422
Query: 832 HNMI 843
HN+I
Sbjct: 423 HNLI 426
>sp|Q86U06|RBM23_HUMAN Probable RNA-binding protein 23 (RNA-binding motif protein 23)
(RNA-binding region containing protein 4) (Splicing
factor SF2)
Length = 439
Score = 61.6 bits (148), Expect = 3e-09
Identities = 46/188 (24%), Positives = 83/188 (44%), Gaps = 6/188 (3%)
Frame = +1
Query: 133 SAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQAEGDPIIAVQINTDKN----- 297
S R AR ++ + + + + DFF+ K++ V+I +D+N
Sbjct: 159 SPEERDARTVFCMQLAARIRPRDLEDFFSAVGKVR-----------DVRIISDRNSRRSK 207
Query: 298 -FAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDTMLSNSLAAGIVQDT 474
A++EF ++ + L G G P+ ++ + ++N+L G
Sbjct: 208 GIAYVEFCEIQSVPLAIGLTGQRLLGVPIIVQASQ----AEKNRLAAMANNLQKG--NGG 261
Query: 475 PHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVKDSSTGLSKGYAFCEYADHSVTDKAC 654
P +L++G L + ED ++ + FG + L+KDS TG SKGY F ++D +A
Sbjct: 262 PMRLYVGSLHFNITEDMLRGIFEPFGKIDNIVLMKDSDTGRSKGYGFITFSDSECARRAL 321
Query: 655 LGLTGMQL 678
L G +L
Sbjct: 322 EQLNGFEL 329
>sp|O43040|CTF1_SCHPO Cleavage and termination factor 1 (Transcription termination factor
ctf1)
Length = 363
Score = 60.8 bits (146), Expect = 4e-09
Identities = 28/73 (38%), Positives = 42/73 (57%)
Frame = +1
Query: 484 LFIGGLPHYLNEDQVKELLLTFGPLSGFNLVKDSSTGLSKGYAFCEYADHSVTDKACLGL 663
+F+G +P+ ++E Q+ E+ GP+ F LV D TG KGY FCE+ D T A L
Sbjct: 9 VFVGNIPYDVSEQQMTEIFNQVGPVKTFKLVLDPETGSGKGYGFCEFFDSETTAMAVRKL 68
Query: 664 TGMQLGEKRIVVQ 702
+LG ++I V+
Sbjct: 69 NNSELGPRKIRVE 81
Score = 36.2 bits (82), Expect = 0.12
Identities = 19/62 (30%), Positives = 32/62 (51%)
Frame = +1
Query: 160 IYVGNIPLGVTEQGMMDFFNEQMKLKLFTQAEGDPIIAVQINTDKNFAFLEFRSVEETSQ 339
++VGNIP V+EQ M + FN+ +K F ++ + + K + F EF E T+
Sbjct: 9 VFVGNIPYDVSEQQMTEIFNQVGPVKTFKL-----VLDPETGSGKGYGFCEFFDSETTAM 63
Query: 340 GL 345
+
Sbjct: 64 AV 65
>sp|Q8BIQ5|CSTF2_MOUSE Cleavage stimulation factor, 64 kDa subunit (CSTF 64 kDa subunit)
(CF-1 64 kDa subunit) (CstF-64)
Length = 580
Score = 60.1 bits (144), Expect = 8e-09
Identities = 27/76 (35%), Positives = 40/76 (52%)
Frame = +1
Query: 484 LFIGGLPHYLNEDQVKELLLTFGPLSGFNLVKDSSTGLSKGYAFCEYADHSVTDKACLGL 663
+F+G +P+ E+Q+K++ GP+ F LV D TG KGY FCEY D A L
Sbjct: 18 VFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCEYQDQETALSAMRNL 77
Query: 664 TGMQLGEKRIVVQRAS 711
G + + + V A+
Sbjct: 78 NGREFSGRALRVDNAA 93
Score = 38.1 bits (87), Expect = 0.031
Identities = 25/91 (27%), Positives = 44/91 (48%), Gaps = 7/91 (7%)
Frame = +1
Query: 136 AVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQAEGDPIIAVQINTD------KN 297
AV R R ++VGNIP TE+ + D F+E P+++ ++ D K
Sbjct: 10 AVDRSLRSVFVGNIPYEATEEQLKDIFSEV-----------GPVVSFRLVYDRETGKPKG 58
Query: 298 FAFLEFRSVEETSQGL-NLDGIMYNGFPLKL 387
+ F E++ E + NL+G ++G L++
Sbjct: 59 YGFCEYQDQETALSAMRNLNGREFSGRALRV 89
>sp|P33240|CSTF2_HUMAN Cleavage stimulation factor, 64 kDa subunit (CSTF 64 kDa subunit)
(CF-1 64 kDa subunit) (CstF-64)
Length = 577
Score = 60.1 bits (144), Expect = 8e-09
Identities = 27/76 (35%), Positives = 40/76 (52%)
Frame = +1
Query: 484 LFIGGLPHYLNEDQVKELLLTFGPLSGFNLVKDSSTGLSKGYAFCEYADHSVTDKACLGL 663
+F+G +P+ E+Q+K++ GP+ F LV D TG KGY FCEY D A L
Sbjct: 18 VFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCEYQDQETALSAMRNL 77
Query: 664 TGMQLGEKRIVVQRAS 711
G + + + V A+
Sbjct: 78 NGREFSGRALRVDNAA 93
Score = 38.1 bits (87), Expect = 0.031
Identities = 25/91 (27%), Positives = 44/91 (48%), Gaps = 7/91 (7%)
Frame = +1
Query: 136 AVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQAEGDPIIAVQINTD------KN 297
AV R R ++VGNIP TE+ + D F+E P+++ ++ D K
Sbjct: 10 AVDRSLRSVFVGNIPYEATEEQLKDIFSEV-----------GPVVSFRLVYDRETGKPKG 58
Query: 298 FAFLEFRSVEETSQGL-NLDGIMYNGFPLKL 387
+ F E++ E + NL+G ++G L++
Sbjct: 59 YGFCEYQDQETALSAMRNLNGREFSGRALRV 89
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 97,408,764
Number of Sequences: 369166
Number of extensions: 1992383
Number of successful extensions: 5123
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4671
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5026
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8245425915
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)