Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_023_P20
(793 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P70619|GSHR_RAT Glutathione reductase (GR) (GRase) 288 9e-78
sp|P47791|GSHR_MOUSE Glutathione reductase, mitochondrial p... 288 2e-77
sp|P00390|GSHR_HUMAN Glutathione reductase, mitochondrial p... 284 2e-76
sp|Q60151|GSHR_STRTR Glutathione reductase (GR) (GRase) 243 6e-64
sp|P06715|GSHR_ECOLI Glutathione reductase (GR) (GRase) 238 2e-62
sp|Q74ZK4|GSHR_ASHGO Glutathione reductase (GR) (GRase) 232 8e-61
sp|Q873E8|GSHR_NEUCR Glutathione reductase (GR) (GRase) 229 5e-60
sp|P43783|GSHR_HAEIN Glutathione reductase (GR) (GRase) 224 3e-58
sp|P78965|GSHR_SCHPO Glutathione reductase (GR) (GRase) 217 3e-56
sp|Q6HA23|GSHR_KLULA Glutathione reductase (GR) (GRase) 216 7e-56
>sp|P70619|GSHR_RAT Glutathione reductase (GR) (GRase)
Length = 424
Score = 288 bits (738), Expect = 9e-78
Identities = 147/270 (54%), Positives = 191/270 (70%), Gaps = 7/270 (2%)
Frame = +3
Query: 3 VNSHHYTADHILIATGGQPVIPN---IPGAHFGITSDGFFELKSLPKKXXXXXXXXXXXE 173
VN +TA HILIATGG P +P+ IPGA GITSDGFF+L+ LP + E
Sbjct: 84 VNGKKFTAPHILIATGGVPTVPHENQIPGASLGITSDGFFQLEDLPSRSVIVGAGYIAVE 143
Query: 174 LAGILNALGSEVTLMARSEIVLRNFDKMIQTIVAENLQSSG-VKLLFKSEVYKINCVDEN 350
+AGIL+ALGS+ +LM R + VLR+FD +I + E L+++G V++L + ++ V +
Sbjct: 144 IAGILSALGSKTSLMIRHDKVLRSFDSLISSNCTEELENAGGVEVLTVKKFSQVKEVKKT 203
Query: 351 SENLEVHVKSNDSGSK---NIISDIDCLIWAIGRVPNTKSLQLDKLGIHTDKNGHIVVDE 521
S LE+HV + G K I D+DCL+WAIGR PN+K L L+KLGI TD GHI+VDE
Sbjct: 204 SSGLELHVVTALPGRKPTVTTIPDVDCLLWAIGRDPNSKGLNLNKLGIQTDDKGHILVDE 263
Query: 522 WQNTSVPGYYAVGDVCGKFLLTPVAIAAGRRLADRLFNGQDERKLDYSNIPTVIFSHPPC 701
+QNT+V G YAVGDVCGK LLTPVAIAAGR+LA RLF G+++ +LDY NIPTV+FSHPP
Sbjct: 264 FQNTNVKGVYAVGDVCGKALLTPVAIAAGRKLAHRLFEGKEDSRLDYDNIPTVVFSHPPI 323
Query: 702 GVIGMTEQEAVSNYGNDKVKVYKTSFKPLF 791
G +G+TE EAV YG D VK+Y T+F P++
Sbjct: 324 GTVGLTEDEAVHKYGKDNVKIYSTAFTPMY 353
>sp|P47791|GSHR_MOUSE Glutathione reductase, mitochondrial precursor (GR) (GRase)
Length = 500
Score = 288 bits (736), Expect = 2e-77
Identities = 146/269 (54%), Positives = 192/269 (71%), Gaps = 6/269 (2%)
Frame = +3
Query: 3 VNSHHYTADHILIATGGQPVIPN---IPGAHFGITSDGFFELKSLPKKXXXXXXXXXXXE 173
VN +TA HILIATGG P +P+ IPGA GITSDGFF+L+ LP + E
Sbjct: 164 VNGKKFTAPHILIATGGVPTVPHESQIPGASLGITSDGFFQLEDLPSRSVIVGAGYIAVE 223
Query: 174 LAGILNALGSEVTLMARSEIVLRNFDKMIQTIVAENLQSSGVKLLFKSEVYKINCVDENS 353
+AGIL+ALGS+ +LM R + VLRNFD +I + E L+++GV++L ++V ++ + S
Sbjct: 224 IAGILSALGSKTSLMIRHDKVLRNFDSLISSNCTEELENAGVEVLKFTQVKEVK---KTS 280
Query: 354 ENLEVHVKSNDSGSK---NIISDIDCLIWAIGRVPNTKSLQLDKLGIHTDKNGHIVVDEW 524
LE+ V ++ G K +I D+DCL+WAIGR PN+K L L+K+GI TD+ GHI+VDE+
Sbjct: 281 SGLELQVVTSVPGRKPTTTMIPDVDCLLWAIGRDPNSKGLNLNKVGIQTDEKGHILVDEF 340
Query: 525 QNTSVPGYYAVGDVCGKFLLTPVAIAAGRRLADRLFNGQDERKLDYSNIPTVIFSHPPCG 704
QNT+V G YAVGDVCGK LLTPVAIAAGR+LA RLF + + KLDY NIPTV+FSHPP G
Sbjct: 341 QNTNVKGVYAVGDVCGKALLTPVAIAAGRKLAHRLFECKQDSKLDYDNIPTVVFSHPPIG 400
Query: 705 VIGMTEQEAVSNYGNDKVKVYKTSFKPLF 791
+G+TE EAV YG D VK+Y T+F P++
Sbjct: 401 TVGLTEDEAVHKYGKDNVKIYSTAFTPMY 429
>sp|P00390|GSHR_HUMAN Glutathione reductase, mitochondrial precursor (GR) (GRase)
Length = 522
Score = 284 bits (727), Expect = 2e-76
Identities = 148/269 (55%), Positives = 189/269 (70%), Gaps = 6/269 (2%)
Frame = +3
Query: 3 VNSHHYTADHILIATGGQPVIPN---IPGAHFGITSDGFFELKSLPKKXXXXXXXXXXXE 173
V+ YTA HILIATGG P P+ IPGA GITSDGFF+L+ LP + E
Sbjct: 186 VSGKKYTAPHILIATGGMPSTPHESQIPGASLGITSDGFFQLEELPGRSVIVGAGYIAVE 245
Query: 174 LAGILNALGSEVTLMARSEIVLRNFDKMIQTIVAENLQSSGVKLLFKSEVYKINCVDENS 353
+AGIL+ALGS+ +LM R + VLR+FD MI T E L+++GV++L S+V ++ +
Sbjct: 246 MAGILSALGSKTSLMIRHDKVLRSFDSMISTNCTEELENAGVEVLKFSQVKEVK---KTL 302
Query: 354 ENLEVHVKSNDSGS---KNIISDIDCLIWAIGRVPNTKSLQLDKLGIHTDKNGHIVVDEW 524
LEV + + G +I D+DCL+WAIGRVPNTK L L+KLGI TD GHI+VDE+
Sbjct: 303 SGLEVSMVTAVPGRLPVMTMIPDVDCLLWAIGRVPNTKDLSLNKLGIQTDDKGHIIVDEF 362
Query: 525 QNTSVPGYYAVGDVCGKFLLTPVAIAAGRRLADRLFNGQDERKLDYSNIPTVIFSHPPCG 704
QNT+V G YAVGDVCGK LLTPVAIAAGR+LA RLF +++ KLDY+NIPTV+FSHPP G
Sbjct: 363 QNTNVKGIYAVGDVCGKALLTPVAIAAGRKLAHRLFEYKEDSKLDYNNIPTVVFSHPPIG 422
Query: 705 VIGMTEQEAVSNYGNDKVKVYKTSFKPLF 791
+G+TE EA+ YG + VK Y TSF P++
Sbjct: 423 TVGLTEDEAIHKYGIENVKTYSTSFTPMY 451
>sp|Q60151|GSHR_STRTR Glutathione reductase (GR) (GRase)
Length = 450
Score = 243 bits (619), Expect = 6e-64
Identities = 119/258 (46%), Positives = 171/258 (66%)
Frame = +3
Query: 18 YTADHILIATGGQPVIPNIPGAHFGITSDGFFELKSLPKKXXXXXXXXXXXELAGILNAL 197
YTA HILIATGG P+ PNIPG+ +GITSDGFFEL +PK+ E+AG+LNAL
Sbjct: 130 YTAPHILIATGGHPLYPNIPGSEYGITSDGFFELDEVPKRTAVIGAGYIAVEVAGVLNAL 189
Query: 198 GSEVTLMARSEIVLRNFDKMIQTIVAENLQSSGVKLLFKSEVYKINCVDENSENLEVHVK 377
GS+ L R + LR FDK I ++ + + SG L + ++ V ++L +
Sbjct: 190 GSDTHLFVRKDRPLRTFDKDIIDVLVDEMAKSGPTLHMHANATEV--VKNADDSLTISFD 247
Query: 378 SNDSGSKNIISDIDCLIWAIGRVPNTKSLQLDKLGIHTDKNGHIVVDEWQNTSVPGYYAV 557
+ ++ + +DCLIWA+GR NT L+K G+ + G+I DE++NTSVPG YA+
Sbjct: 248 NEETIT------VDCLIWAVGRAANTSGFGLEKTGVELTERGNIYSDEFENTSVPGIYAL 301
Query: 558 GDVCGKFLLTPVAIAAGRRLADRLFNGQDERKLDYSNIPTVIFSHPPCGVIGMTEQEAVS 737
GDV GK LTPVA+ AGR+L++RLFN + + KLDY+++ TV+FSHP G IG+TE++A++
Sbjct: 302 GDVTGKLDLTPVAVKAGRQLSERLFNNKVDAKLDYTDVATVVFSHPAIGAIGLTEEKAIA 361
Query: 738 NYGNDKVKVYKTSFKPLF 791
YG + +KVYK+SF P++
Sbjct: 362 KYGAENIKVYKSSFTPMY 379
>sp|P06715|GSHR_ECOLI Glutathione reductase (GR) (GRase)
Length = 450
Score = 238 bits (606), Expect = 2e-62
Identities = 121/263 (46%), Positives = 169/263 (64%)
Frame = +3
Query: 3 VNSHHYTADHILIATGGQPVIPNIPGAHFGITSDGFFELKSLPKKXXXXXXXXXXXELAG 182
VN TADHILIATGG+P P+IPG +GI SDGFF L +LP++ ELAG
Sbjct: 125 VNGETITADHILIATGGRPSHPDIPGVEYGIDSDGFFALPALPERVAVVGAGYIAVELAG 184
Query: 183 ILNALGSEVTLMARSEIVLRNFDKMIQTIVAENLQSSGVKLLFKSEVYKINCVDENSENL 362
++N LG++ L R LR+FD MI + E + + G +L + + + S L
Sbjct: 185 VINGLGAKTHLFVRKHAPLRSFDPMISETLVEVMNAEGPQLHTNAIPKAVVKNTDGSLTL 244
Query: 363 EVHVKSNDSGSKNIISDIDCLIWAIGRVPNTKSLQLDKLGIHTDKNGHIVVDEWQNTSVP 542
E+ +++ +DCLIWAIGR P ++ L+ G+ T++ G+IVVD++QNT++
Sbjct: 245 ELEDGRSET--------VDCLIWAIGREPANDNINLEAAGVKTNEKGYIVVDKYQNTNIE 296
Query: 543 GYYAVGDVCGKFLLTPVAIAAGRRLADRLFNGQDERKLDYSNIPTVIFSHPPCGVIGMTE 722
G YAVGD G LTPVA+AAGRRL++RLFN + + LDYSNIPTV+FSHPP G +G+TE
Sbjct: 297 GIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHLDYSNIPTVVFSHPPIGTVGLTE 356
Query: 723 QEAVSNYGNDKVKVYKTSFKPLF 791
+A YG+D+VKVYK+SF ++
Sbjct: 357 PQAREQYGDDQVKVYKSSFTAMY 379
>sp|Q74ZK4|GSHR_ASHGO Glutathione reductase (GR) (GRase)
Length = 480
Score = 232 bits (592), Expect = 8e-61
Identities = 121/261 (46%), Positives = 165/261 (63%), Gaps = 3/261 (1%)
Frame = +3
Query: 18 YTADHILIATGGQPVIPN-IPGAHFGITSDGFFELKSLPKKXXXXXXXXXXXELAGILNA 194
YTADHILIATGG+PV+P IPGA +G+ SDGFF L+ PKK E AG+ N
Sbjct: 157 YTADHILIATGGEPVLPEGIPGAEYGVDSDGFFRLEEQPKKVVISGSGYIATEFAGVFNG 216
Query: 195 LGSEVTLMARSEIVLRNFDKMIQTIVAENLQSSGVKLLFKSEVYKINCVDENSENLEVHV 374
LG+E ++ R + VL FD IQ IV E+ + GV + K + ++ D N+ L VH+
Sbjct: 217 LGTETHIVIRKDHVLTKFDPSIQEIVTEHYEKEGVNIHKKESIQRVE-KDPNTGKLTVHL 275
Query: 375 KSNDSGSKNIISDIDCLIWAIGRVPNTKSLQLDKLGIHTDKNGHIVVDEWQNTSVPGYYA 554
S II D+D L+WAIGR + + + +G+ + GH+VVDE+QNTS G YA
Sbjct: 276 ------SGKIIEDVDQLVWAIGR-KSLLGIAPENVGVKLGETGHVVVDEYQNTSTKGIYA 328
Query: 555 VGDVCGKFLLTPVAIAAGRRLADRLFNGQDER--KLDYSNIPTVIFSHPPCGVIGMTEQE 728
+GDV G LTPVAIAAGR+LA+RLF + R K DY N+P+V+FSHP G IG+TE +
Sbjct: 329 LGDVVGNMELTPVAIAAGRKLANRLFGPEQMRAQKQDYDNVPSVVFSHPEAGSIGLTEPQ 388
Query: 729 AVSNYGNDKVKVYKTSFKPLF 791
A+ YG + +K+Y+T F ++
Sbjct: 389 AIERYGKENIKIYQTKFTAMY 409
>sp|Q873E8|GSHR_NEUCR Glutathione reductase (GR) (GRase)
Length = 468
Score = 229 bits (585), Expect = 5e-60
Identities = 122/259 (47%), Positives = 169/259 (65%), Gaps = 3/259 (1%)
Frame = +3
Query: 24 ADHILIATGGQPVIP-NIPGAHFGITSDGFFELKSLPKKXXXXXXXXXXXELAGILNALG 200
A ILIA GG P IP +IPG+ +G SDGFF++ +LPKK E AG+LNALG
Sbjct: 141 AKKILIAVGGNPTIPPHIPGSEYGTNSDGFFDIDTLPKKVALVGAGYIAVEFAGMLNALG 200
Query: 201 SEVTLMARSEIVLRNFDKMIQTIVAENLQSSGVKLLFKSEVYKINCVDENSENLEVHVKS 380
E L R + LR+FD MIQ + + + GVK+ KS++ + + + L ++ K
Sbjct: 201 VETHLFIRHDTFLRSFDPMIQQVSVKEYERIGVKVHKKSQLTSVQ--KDAAGKLAINFKE 258
Query: 381 NDSGSKNIISDIDCLIWAIGRVPNTKSLQLDKLGIHTDKNGHIVVDEWQNTSVPGYYAVG 560
+ + ISD+D LIWA+GR P + L LDK G+ T++ G+I VDE+QNTS YAVG
Sbjct: 259 GEG--EQSISDVDHLIWAVGRTPAVEGLGLDKAGVKTNEKGYIEVDEYQNTSTENIYAVG 316
Query: 561 DVCGKFLLTPVAIAAGRRLADRLFNGQDER--KLDYSNIPTVIFSHPPCGVIGMTEQEAV 734
DVCG+ LTPVAIAAGR+LA RLF ++ R KL+Y N+P+V+F+HP G IG+TE EAV
Sbjct: 317 DVCGQVELTPVAIAAGRKLAARLFGPEEFRTLKLNYDNVPSVVFAHPEIGSIGLTEPEAV 376
Query: 735 SNYGNDKVKVYKTSFKPLF 791
+ YG + +K+YK+SF ++
Sbjct: 377 AKYGAENLKIYKSSFTAMY 395
>sp|P43783|GSHR_HAEIN Glutathione reductase (GR) (GRase)
Length = 456
Score = 224 bits (570), Expect = 3e-58
Identities = 116/258 (44%), Positives = 163/258 (63%), Gaps = 1/258 (0%)
Frame = +3
Query: 21 TADHILIATGGQPVIPNIPGAHFGITSDGFFELKSLPKKXXXXXXXXXXXELAGILNALG 200
TADHILIATGG+P PNI G +GI SDGFF L LPK+ EL+G+LN+LG
Sbjct: 136 TADHILIATGGRPYRPNIKGQEYGIDSDGFFALTELPKRAAVIGAGYIAVELSGVLNSLG 195
Query: 201 SEVTLMARSEIVLRNFDKMIQTIVAENLQSSGVKLLFKSEVYKINCVDENSENLEVHVKS 380
E L+ R +RN D +I + E L G++L S +I +N + +
Sbjct: 196 VETHLLVRRHAPMRNQDPLIVETLVEVLAQDGIQLHTNSTPSEI------VKNADGSLTV 249
Query: 381 NDSGSKNIISDIDCLIWAIGRVPNTKSLQLDKLGIHTDKNGHIVVDEWQNTSVPGYYAVG 560
G ++ +DC+IWA GRVP T + L+ G+ T+++G++ VD++QNT+V G YAVG
Sbjct: 250 RCDGQSDVT--VDCVIWAAGRVPTTDKIGLENAGVETNEHGYVKVDKYQNTNVKGIYAVG 307
Query: 561 DVCGKFL-LTPVAIAAGRRLADRLFNGQDERKLDYSNIPTVIFSHPPCGVIGMTEQEAVS 737
D+ + LTPVA+AAGRRL++RLFN + LDYS +PTV+FSHPP G +G+TE +A+
Sbjct: 308 DIIENGIELTPVAVAAGRRLSERLFNNKPTEYLDYSLVPTVVFSHPPIGTVGLTEPQAIE 367
Query: 738 NYGNDKVKVYKTSFKPLF 791
YG + VKVYK+SF ++
Sbjct: 368 QYGAENVKVYKSSFTAMY 385
>sp|P78965|GSHR_SCHPO Glutathione reductase (GR) (GRase)
Length = 464
Score = 217 bits (552), Expect = 3e-56
Identities = 115/258 (44%), Positives = 158/258 (61%), Gaps = 1/258 (0%)
Frame = +3
Query: 18 YTADHILIATGGQPVIPN-IPGAHFGITSDGFFELKSLPKKXXXXXXXXXXXELAGILNA 194
++A +ILIA GG P+ P+ IPGA +GI SDGFFEL+S PK+ ELAG+ A
Sbjct: 140 FSAKYILIAVGGHPIWPSHIPGAEYGIDSDGFFELESQPKRVAIVGAGYIAVELAGVFAA 199
Query: 195 LGSEVTLMARSEIVLRNFDKMIQTIVAENLQSSGVKLLFKSEVYKINCVDENSENLEVHV 374
LG+E + R LR FD +I + ++ Q G+ + S +K E + E+ +
Sbjct: 200 LGTETHMFIRQSKFLRKFDPIISDGIMDHFQHIGINVHTNSLEFK---KVEKLPSGELCI 256
Query: 375 KSNDSGSKNIISDIDCLIWAIGRVPNTKSLQLDKLGIHTDKNGHIVVDEWQNTSVPGYYA 554
D + N+ D L+WAIGR P + L+L+K G+ T NG I+ D +Q T+VP +
Sbjct: 257 HQQDGSTFNV----DTLLWAIGRAPKIQGLRLEKAGVKTLPNGIIIADTYQRTNVPTVLS 312
Query: 555 VGDVCGKFLLTPVAIAAGRRLADRLFNGQDERKLDYSNIPTVIFSHPPCGVIGMTEQEAV 734
+GDVCGK LTPVAIAAGRRL+DRLF G + LDY +P+V+F+HP G IG+TEQEA+
Sbjct: 313 LGDVCGKLELTPVAIAAGRRLSDRLFGGIKDAHLDYEEVPSVVFAHPEAGTIGLTEQEAI 372
Query: 735 SNYGNDKVKVYKTSFKPL 788
YG ++KVY T F L
Sbjct: 373 DKYGESQIKVYNTKFNGL 390
>sp|Q6HA23|GSHR_KLULA Glutathione reductase (GR) (GRase)
Length = 484
Score = 216 bits (549), Expect = 7e-56
Identities = 118/261 (45%), Positives = 163/261 (62%), Gaps = 3/261 (1%)
Frame = +3
Query: 18 YTADHILIATGGQPVIP-NIPGAHFGITSDGFFELKSLPKKXXXXXXXXXXXELAGILNA 194
YTADHIL+ATGG+P+ P IPG +G++SD FFEL+ PKK E+AG+ N
Sbjct: 158 YTADHILVATGGKPIYPAKIPGYDYGVSSDEFFELEDQPKKVVVVGAGYIGVEIAGVFNG 217
Query: 195 LGSEVTLMARSEIVLRNFDKMIQTIVAENLQSSGVKLLFKSEVYKINCVDENSENLEVHV 374
LGS+ L+ R E VLR FD IQ V + GV + S V K+ DE++ L + +
Sbjct: 218 LGSDSHLVIRGETVLRKFDDCIQETVTDTYIKEGVNIHKSSNVTKVE-KDESTGKLNIQL 276
Query: 375 KSNDSGSKNIISDIDCLIWAIGRVPNTKSLQLDKLGIHTDKNGHIVVDEWQNTSVPGYYA 554
D+G I ++D LIW IGR + L L+ +G+ D IVVDE+QN+SV Y+
Sbjct: 277 ---DTGKN--IDNVDSLIWTIGR-RSLLGLGLENIGVKLDAKEQIVVDEYQNSSVKNVYS 330
Query: 555 VGDVCGKFLLTPVAIAAGRRLADRLFNGQ--DERKLDYSNIPTVIFSHPPCGVIGMTEQE 728
+GDV GK LTPVAIAAGR+L++RLF + +K DY N+P+V+FSHP G IG++E+E
Sbjct: 331 LGDVVGKVELTPVAIAAGRKLSNRLFGPEKFKNQKQDYENVPSVVFSHPEAGSIGLSERE 390
Query: 729 AVSNYGNDKVKVYKTSFKPLF 791
A+ +G D VKVY + F ++
Sbjct: 391 AIEKFGKDNVKVYNSKFNAMY 411
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88,623,166
Number of Sequences: 369166
Number of extensions: 1767949
Number of successful extensions: 5053
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4610
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4772
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7425705210
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)