Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_023_P15
(891 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8R4Z9|MFN1_RAT Transmembrane GTPase MFN1 (Mitofusin-1) ... 134 3e-31
sp|Q811U4|MFN1_MOUSE Transmembrane GTPase MFN1 (Mitofusin-1) 134 4e-31
sp|Q8R500|MFN2_RAT Transmembrane GTPase MFN2 (Mitofusin-2) ... 131 3e-30
sp|Q80U63|MFN2_MOUSE Transmembrane GTPase MFN2 (Mitofusin-2... 129 8e-30
sp|O95140|MFN2_HUMAN Transmembrane GTPase MFN2 (Mitofusin-2) 126 7e-29
sp|Q8IWA4|MFN1_HUMAN Transmembrane GTPase MFN1 (Mitofusin-1... 125 2e-28
sp|Q7YU24|MARF_DROME Transmembrane GTPase Marf (Mitochondri... 122 1e-27
sp|Q23424|FZO1_CAEEL Transmembrane GTPase fzo-1 89 1e-17
sp|Q9N6P4|FZO_DROSI Transmembrane GTPase fzo (Fuzzy onions ... 81 3e-15
sp|O18412|FZO_DROME Transmembrane GTPase fzo (Fuzzy onions ... 76 1e-13
>sp|Q8R4Z9|MFN1_RAT Transmembrane GTPase MFN1 (Mitofusin-1) (Mitochondrial transmembrane
GTPase FZO1B)
Length = 741
Score = 134 bits (337), Expect = 3e-31
Identities = 82/288 (28%), Positives = 139/288 (48%), Gaps = 21/288 (7%)
Frame = +2
Query: 11 LLSKYKVKLSQHVERQLGNNLTSILARDLCSQVDLVSNSIIKKYLSLLPDKEVQEMMRQT 190
+L YK +L++H+E +G NL ++ + + II+ LLP ++
Sbjct: 437 VLKVYKSELNKHIEDGMGRNLADRCTSEVNASILQSQQEIIENLKPLLPAGIQNKLHTLI 496
Query: 191 AYNDYAPQLYVDCNNISSDFHEDLTFRFSLGLTAILGKLIGPIAPGR------------- 331
+ ++C+ + SDF ED+ FRFSLG ++++ + +G R
Sbjct: 497 PCKKFDLSYDLNCHKLCSDFQEDIVFRFSLGWSSLVHRFLGSTNAQRVLLGLSEPIFQVP 556
Query: 332 --------QPHTXXXXXXXXXXXXXXAVRQMLPNITGQTVLVTVLCGGLMLRATGWKVLA 487
P + L ++T +T + ++ GG++ + GWK+++
Sbjct: 557 RSLASTPTAPSNPAAPDNAAQEELMITLITGLASLTSRTSMGIIVVGGVIWKTVGWKLIS 616
Query: 488 GVGCIYAAMYGYEYSTWTTRAKEKAFKTQFRSYVAEKLELVMAYTSKNSADQPQNALNRT 667
+Y A+Y YE TWTTRAKE+AFK QF +Y EKL++++ +TS N + Q Q + T
Sbjct: 617 VTLSMYGALYLYERLTWTTRAKERAFKQQFVNYATEKLQMIVKFTSANCSHQVQQEMATT 676
Query: 668 LKSLDSVVDKHITKLKTDITRFEGDVKHLEEIVLKSKNLKNKAIYIER 811
L VD L+ +I R ++ LE+I SK L+NKA+ +ER
Sbjct: 677 FARLCQQVDVTQKHLEEEIARLSKEIDQLEKIQNNSKLLRNKAVQLER 724
>sp|Q811U4|MFN1_MOUSE Transmembrane GTPase MFN1 (Mitofusin-1)
Length = 741
Score = 134 bits (336), Expect = 4e-31
Identities = 82/287 (28%), Positives = 139/287 (48%), Gaps = 21/287 (7%)
Frame = +2
Query: 11 LLSKYKVKLSQHVERQLGNNLTSILARDLCSQVDLVSNSIIKKYLSLLPDKEVQEMMRQT 190
+L YK +L++H+E +G NL ++ + + II+ LLP ++
Sbjct: 437 VLKVYKSELNKHIEDGMGRNLADRCTNEVNASILQSQQEIIENLKPLLPAGIQNKLHTLI 496
Query: 191 AYNDYAPQLYVDCNNISSDFHEDLTFRFSLGLTAILGKLIGPIAPGR------------- 331
+ ++C+ + SDF ED+ FRFSLG ++++ + +G R
Sbjct: 497 PCKKFDLSYDLNCHKLCSDFQEDIVFRFSLGWSSLVHRFLGSTNAQRVLLGLSEPIFQVP 556
Query: 332 --------QPHTXXXXXXXXXXXXXXAVRQMLPNITGQTVLVTVLCGGLMLRATGWKVLA 487
P + L ++T +T + ++ GG++ + GWK+++
Sbjct: 557 RSLASTPTAPSNPAAPDNAAQEELMITLITGLASLTSRTSMGIIVVGGVIWKTVGWKLIS 616
Query: 488 GVGCIYAAMYGYEYSTWTTRAKEKAFKTQFRSYVAEKLELVMAYTSKNSADQPQNALNRT 667
+Y A+Y YE TWTTRAKE+AFK QF +Y EKL++++++TS N + Q Q + T
Sbjct: 617 VTLSMYGALYLYERLTWTTRAKERAFKQQFVNYATEKLQMIVSFTSANCSHQVQQEMATT 676
Query: 668 LKSLDSVVDKHITKLKTDITRFEGDVKHLEEIVLKSKNLKNKAIYIE 808
L VD L+ +I R ++ LE+I SK L+NKAI +E
Sbjct: 677 FARLCQQVDVTQKHLEEEIARLSKEIDQLEKIQNNSKLLRNKAIQLE 723
>sp|Q8R500|MFN2_RAT Transmembrane GTPase MFN2 (Mitofusin-2) (Mitochondrial transmembrane
GTPase FZO1A) (HSG protein)
Length = 757
Score = 131 bits (329), Expect = 3e-30
Identities = 79/285 (27%), Positives = 139/285 (48%), Gaps = 19/285 (6%)
Frame = +2
Query: 11 LLSKYKVKLSQHVERQLGNNLTSILARDLCSQVDLVSNSIIKKYLSLLPDKEVQEMMRQT 190
+L YK +L +H+E LG N++ + + S + + +I LLP ++
Sbjct: 458 VLKVYKNELHRHIEEGLGRNMSDRCSTAIASSLQTMQQDMIDGLKPLLPVSVRNQIDMLV 517
Query: 191 AYNDYAPQLYVDCNNISSDFHEDLTFRFSLGLTAILGKLIGP------------------ 316
++ ++C+ + +DF ED+ F FSLG T ++ + +GP
Sbjct: 518 PRQCFSLSYDLNCDKLCADFQEDIEFHFSLGWTMLVNRFLGPKNSRRALLGYNDQVQRPL 577
Query: 317 -IAPGRQPHTXXXXXXXXXXXXXXAVRQMLPNITGQTVLVTVLCGGLMLRATGWKVLAGV 493
+ P ++ L ++T +T + ++ GG++ +A GW+++A
Sbjct: 578 PLTPANPSMPPLPQGSLTQEELMVSMVTGLASLTSRTSMGILVVGGVVWKAVGWRLIALS 637
Query: 494 GCIYAAMYGYEYSTWTTRAKEKAFKTQFRSYVAEKLELVMAYTSKNSADQPQNALNRTLK 673
+Y +Y YE TWTTRAKE+AFK QF Y +EKL+L+++YT N + Q Q L+ T
Sbjct: 638 FGLYGLLYVYERLTWTTRAKERAFKRQFVEYASEKLQLIISYTGSNCSHQVQQELSGTFA 697
Query: 674 SLDSVVDKHITKLKTDITRFEGDVKHLEEIVLKSKNLKNKAIYIE 808
L VD L+ +I V+ L+ + K+K L+NKA +++
Sbjct: 698 HLCQQVDITRDNLEQEIAAMNKKVEALDSLQSKAKLLRNKAGWLD 742
>sp|Q80U63|MFN2_MOUSE Transmembrane GTPase MFN2 (Mitofusin-2) (Hypertension related protein
1) (Mitochondrial assembly regulatory factor)
Length = 757
Score = 129 bits (325), Expect = 8e-30
Identities = 78/285 (27%), Positives = 139/285 (48%), Gaps = 19/285 (6%)
Frame = +2
Query: 11 LLSKYKVKLSQHVERQLGNNLTSILARDLCSQVDLVSNSIIKKYLSLLPDKEVQEMMRQT 190
+L YK +L +H+E LG NL+ + + S + + +I LLP ++
Sbjct: 458 VLKVYKNELHRHIEEGLGRNLSDRCSTAIASSLQTMQQDMIDGLKPLLPVSMRNQIDMLV 517
Query: 191 AYNDYAPQLYVDCNNISSDFHEDLTFRFSLGLTAILGKLIGP------------------ 316
++ ++C+ + +DF ED+ F FSLG T ++ + +GP
Sbjct: 518 PRQCFSLSYDLNCDKLCADFQEDIEFHFSLGWTMLVNRFLGPKNSRRALLGYSDQVQRPL 577
Query: 317 -IAPGRQPHTXXXXXXXXXXXXXXAVRQMLPNITGQTVLVTVLCGGLMLRATGWKVLAGV 493
+ P ++ L ++T +T + ++ GG++ +A GW+++A
Sbjct: 578 PLTPANPSMPPLPQSSLTQEELMVSMVTGLASLTSRTSMGILVVGGVVWKAVGWRLIALS 637
Query: 494 GCIYAAMYGYEYSTWTTRAKEKAFKTQFRSYVAEKLELVMAYTSKNSADQPQNALNRTLK 673
+Y +Y YE TWTT+AKE+AFK QF Y +EKL+L+++YT N + Q Q L+ T
Sbjct: 638 FGLYGLLYVYERLTWTTKAKERAFKRQFVEYASEKLQLIISYTGSNCSHQVQQELSGTFA 697
Query: 674 SLDSVVDKHITKLKTDITRFEGDVKHLEEIVLKSKNLKNKAIYIE 808
L VD L+ +I V+ L+ + ++K L+NKA +++
Sbjct: 698 HLCQQVDITRDNLEQEIAAMNKKVEALDSLQSRAKLLRNKAGWLD 742
>sp|O95140|MFN2_HUMAN Transmembrane GTPase MFN2 (Mitofusin-2)
Length = 757
Score = 126 bits (317), Expect = 7e-29
Identities = 76/285 (26%), Positives = 139/285 (48%), Gaps = 19/285 (6%)
Frame = +2
Query: 11 LLSKYKVKLSQHVERQLGNNLTSILARDLCSQVDLVSNSIIKKYLSLLPDKEVQEMMRQT 190
+L YK +L +H+E LG N++ + + + + + +I LLP ++
Sbjct: 458 VLKVYKNELHRHIEEGLGRNMSDRCSTAITNSLQTMQQDMIDGLKPLLPVSVRSQIDMLV 517
Query: 191 AYNDYAPQLYVDCNNISSDFHEDLTFRFSLGLTAILGKLIGP------------------ 316
++ ++C+ + +DF ED+ F FSLG T ++ + +GP
Sbjct: 518 PRQCFSLNYDLNCDKLCADFQEDIEFHFSLGWTMLVNRFLGPKNSRRALMGYNDQVQRPI 577
Query: 317 -IAPGRQPHTXXXXXXXXXXXXXXAVRQMLPNITGQTVLVTVLCGGLMLRATGWKVLAGV 493
+ P ++ L ++T +T + ++ GG++ +A GW+++A
Sbjct: 578 PLTPANPSMPPLPQGSLTQEEFMVSMVTGLASLTSRTSMGILVVGGVVWKAVGWRLIALS 637
Query: 494 GCIYAAMYGYEYSTWTTRAKEKAFKTQFRSYVAEKLELVMAYTSKNSADQPQNALNRTLK 673
+Y +Y YE TWTT+AKE+AFK QF + +EKL+LV++YT N + Q Q L+ T
Sbjct: 638 FGLYGLLYVYERLTWTTKAKERAFKRQFVEHASEKLQLVISYTGSNCSHQVQQELSGTFA 697
Query: 674 SLDSVVDKHITKLKTDITRFEGDVKHLEEIVLKSKNLKNKAIYIE 808
L VD L+ +I ++ L+ + K+K L+NKA +++
Sbjct: 698 HLCQQVDVTRENLEQEIAAMNKKIEVLDSLQSKAKLLRNKAGWLD 742
>sp|Q8IWA4|MFN1_HUMAN Transmembrane GTPase MFN1 (Mitofusin-1) (Fzo homolog)
Length = 741
Score = 125 bits (314), Expect = 2e-28
Identities = 81/287 (28%), Positives = 138/287 (48%), Gaps = 21/287 (7%)
Frame = +2
Query: 11 LLSKYKVKLSQHVERQLGNNLTSILARDLCSQVDLVSNSIIKKYLSLLPDKEVQEMMRQT 190
+L YK +L++H+E +G NL ++ + V II+ LLP ++
Sbjct: 437 VLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEIIENLKPLLPAGIQDKLHTLI 496
Query: 191 AYNDYAPQLYVDCNNISSDFHEDLTFRFSLGLTAILGKLIGPIAPGR------------- 331
+ ++ + + SDF ED+ FRFSLG ++++ + +GP R
Sbjct: 497 PCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLGPRNAQRVLLGLSEPIFQLP 556
Query: 332 --------QPHTXXXXXXXXXXXXXXAVRQMLPNITGQTVLVTVLCGGLMLRATGWKVLA 487
P T + L ++T +T + ++ GG++ + GWK+L+
Sbjct: 557 RSLASTPTAPTTPATPDNASQEELMITLVTGLASVTSRTSMGIIIVGGVIWKTIGWKLLS 616
Query: 488 GVGCIYAAMYGYEYSTWTTRAKEKAFKTQFRSYVAEKLELVMAYTSKNSADQPQNALNRT 667
+Y A+Y YE +WTT AKE+AFK QF +Y EKL ++++ TS N + Q + + T
Sbjct: 617 VSLTMYGALYLYERLSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATT 676
Query: 668 LKSLDSVVDKHITKLKTDITRFEGDVKHLEEIVLKSKNLKNKAIYIE 808
L VD +L+ +I R ++ LE+I SK L+NKA+ +E
Sbjct: 677 FARLCQQVDITQKQLEEEIARLPKEIDQLEKIQNNSKLLRNKAVQLE 723
>sp|Q7YU24|MARF_DROME Transmembrane GTPase Marf (Mitochondrial assembly regulatory factor)
(Mitofusin)
Length = 810
Score = 122 bits (306), Expect = 1e-27
Identities = 84/299 (28%), Positives = 140/299 (46%), Gaps = 33/299 (11%)
Frame = +2
Query: 11 LLSKYKVKLSQHVERQLGNNLTSILARDLCSQVDLVSNSIIKKYLSLLPDKEV-QEMMRQ 187
+L+ YK +L+ HVE LG+NL + L+ L V+ + + +L+P++++ +
Sbjct: 498 VLNIYKKELNAHVESGLGSNLRARLSMALAMNVESAQTEMTDRMHALVPNEQLLATSTKM 557
Query: 188 TAYNDYAPQLY-VDCNNISSDFHEDLTFRFSLGLTAILGKLIG----------PIAPGRQ 334
LY ++C N+ +DF EDL F+FS G+ A++ + G P RQ
Sbjct: 558 VVRTQPFEMLYSLNCQNLCADFQEDLEFKFSWGIAAMIQRFTGKVRERSKKGQPALVNRQ 617
Query: 335 ---------PHTXXXXXXXXXXXXXXAVRQMLP------------NITGQTVLVTVLCGG 451
P T V + P +I Q + ++ G
Sbjct: 618 SSIGHSVSTPTTTPVEATPVCLLPAPVVAGITPEQLSLISRFAVSSIGSQGTVGGLVVAG 677
Query: 452 LMLRATGWKVLAGVGCIYAAMYGYEYSTWTTRAKEKAFKTQFRSYVAEKLELVMAYTSKN 631
+ML+ GW+VL GVG +Y +Y YE +WT AKE+ FK+Q+ + +KL++++ TS N
Sbjct: 678 VMLKTIGWRVLVGVGALYGCIYLYERLSWTNSAKERTFKSQYVRHATKKLKMIVDLTSAN 737
Query: 632 SADQPQNALNRTLKSLDSVVDKHITKLKTDITRFEGDVKHLEEIVLKSKNLKNKAIYIE 808
+ Q Q L+ T L VD T + ++ + + LE + K L+NKA YI+
Sbjct: 738 CSHQVQQELSSTFARLCRTVDTATTDMNDELKTLDSQLNILEANQKQLKLLRNKANYIQ 796
>sp|Q23424|FZO1_CAEEL Transmembrane GTPase fzo-1
Length = 774
Score = 89.4 bits (220), Expect = 1e-17
Identities = 66/290 (22%), Positives = 132/290 (45%), Gaps = 24/290 (8%)
Frame = +2
Query: 14 LSKYKVKLSQHVERQLGNNLTSILARDLCSQVDLVSNSIIKKYLSLLPD---KEVQEMMR 184
++KYK L+ V++ L ++L + L S++ + N + + +L + +++E+ R
Sbjct: 469 MTKYKEDLAIFVDKCLSSDLEARCTGGLMSRIWNLENDMFQYVTKILAEPYQNKLEEVWR 528
Query: 185 QTAYNDYAPQLYVDCNNISSDFHEDLTFRFSLGLTAILGKLIG----------------P 316
A ++ + VD + +DFHEDL FRF+ GL AI+ ++I P
Sbjct: 529 YRAPFKFS--ICVDVPALVNDFHEDLEFRFTFGLHAIIRRIIAYRSGQPVTAINTNLLTP 586
Query: 317 IAPGRQPHTXXXXXXXXXXXXXXAVRQMLPNITGQTVLVT-----VLCGGLMLRATGWKV 481
++ +Q +T L ++ GG++ +A GW+V
Sbjct: 587 LSLKQQSEKNSVRDAEASAASEEQAMMTQMVLTSAAFLANGSLGVLVVGGIVYKAVGWRV 646
Query: 482 LAGVGCIYAAMYGYEYSTWTTRAKEKAFKTQFRSYVAEKLELVMAYTSKNSADQPQNALN 661
+A G YA +Y +E W + AKE+ K QFRS++A +++ V + + Q ++
Sbjct: 647 IAVGGAAYAGLYAWERMRWNSGAKEQHLKEQFRSHLAARMQQVSTAHTHHCETQAIREMD 706
Query: 662 RTLKSLDSVVDKHITKLKTDITRFEGDVKHLEEIVLKSKNLKNKAIYIER 811
+ L + V ++K D+ + + ++ + +K KA+++ R
Sbjct: 707 QVFDGLKATVGGVHREMKNDLDVQKTQIDAVDSTIRTLGTIKGKAVFLLR 756
>sp|Q9N6P4|FZO_DROSI Transmembrane GTPase fzo (Fuzzy onions protein)
Length = 454
Score = 81.3 bits (199), Expect = 3e-15
Identities = 58/250 (23%), Positives = 109/250 (43%), Gaps = 15/250 (6%)
Frame = +2
Query: 89 RDLCSQVDLVSNSIIKKYLSLLPDKEVQEMMRQTAYN------DYAPQLYVDCNNISSDF 250
R LC+ +D + + + LS+ +++ +M ++ D+ +DC + SDF
Sbjct: 199 RSLCAHLDNLLEDRVLQCLSIPLQRKILDMEKELGLQITEKSCDWQLIYGLDCQSYMSDF 258
Query: 251 HEDLTFRFSLGLTAILGKLIGPIAPGRQP---------HTXXXXXXXXXXXXXXAVRQML 403
DL FRFSLG TA+ +L G + P H + + L
Sbjct: 259 QPDLRFRFSLGFTALWHRLEGNLPLHSSPFRTQKLRNGHKKCLPLPPLVHGNHWQMLESL 318
Query: 404 PNITGQTVLVTVLCGGLMLRATGWKVLAGVGCIYAAMYGYEYSTWTTRAKEKAFKTQFRS 583
G L TVL G + +R+ W ++ +G + + Y YEY+ WTT A+E++FK+Q+
Sbjct: 319 VKSKGS--LGTVLLGAMAIRSFNWPIVMILGGLVGSFYMYEYAAWTTAAQERSFKSQYSR 376
Query: 584 YVAEKLELVMAYTSKNSADQPQNALNRTLKSLDSVVDKHITKLKTDITRFEGDVKHLEEI 763
+ ++L + T Q + L + ++ ++ + L ++ +E +
Sbjct: 377 LLQQRLRTDVQQTVSGFELQLRQHLAKVRNCWEAQSNETLNDLNVRTAELTKQIQSMEVL 436
Query: 764 VLKSKNLKNK 793
L K ++K
Sbjct: 437 QLSLKKFRDK 446
>sp|O18412|FZO_DROME Transmembrane GTPase fzo (Fuzzy onions protein)
Length = 718
Score = 76.3 bits (186), Expect = 1e-13
Identities = 59/250 (23%), Positives = 109/250 (43%), Gaps = 15/250 (6%)
Frame = +2
Query: 89 RDLCSQVDLVSNSIIKKYLSLLPDKEVQEMMRQT----AYNDYAPQLY--VDCNNISSDF 250
R LC+ +D + + + LS+ +++ ++ ++ A N QL +DC + SDF
Sbjct: 452 RSLCAHLDKLLEDRVLQCLSIPLQRKILDIEKEIGLPIAENSCDWQLIYGLDCQSYMSDF 511
Query: 251 HEDLTFRFSLGLTAILGKLIGPIAPGRQP---------HTXXXXXXXXXXXXXXAVRQML 403
DL FRFSLG TA+ +L G + P H + + L
Sbjct: 512 QPDLRFRFSLGFTALWHRLEGNLPLHASPFRIQKLQNGHKKCSPLPPLVNGNHWQMLESL 571
Query: 404 PNITGQTVLVTVLCGGLMLRATGWKVLAGVGCIYAAMYGYEYSTWTTRAKEKAFKTQFRS 583
G L TVL + +R+ W ++ +G + + Y YEY+ WTT A+E++FK+Q+
Sbjct: 572 VKSKGS--LGTVLLSAMAIRSFNWPIVLILGGLVGSFYIYEYAAWTTAAQERSFKSQYAR 629
Query: 584 YVAEKLELVMAYTSKNSADQPQNALNRTLKSLDSVVDKHITKLKTDITRFEGDVKHLEEI 763
+ ++L + T Q + L ++ ++ + L ++ +E +
Sbjct: 630 LLQQRLRSDVQQTVSGFELQLRQHLATVRNCWEAQSNETLNDLNVRTAELTKQIQSMEVL 689
Query: 764 VLKSKNLKNK 793
L K ++K
Sbjct: 690 QLSLKKFRDK 699
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 100,853,386
Number of Sequences: 369166
Number of extensions: 2079623
Number of successful extensions: 6676
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6402
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6661
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8934348180
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)