Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_023_P10
(462 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P51534|SHE4_YEAST Protein SHE4 32 0.90
sp|P38041|BOB1_YEAST Protein BOB1 (BEM1-binding protein) 31 1.2
sp|O13759|CSX1_SCHPO RNA-binding post-transcriptional regul... 31 1.2
sp|O43493|TGON2_HUMAN Trans-Golgi network integral membrane... 30 2.6
sp|P25386|USO1_YEAST Intracellular protein transport protei... 29 4.5
sp|P12036|NFH_HUMAN Neurofilament triplet H protein (200 kD... 29 4.5
sp|O01761|UNC89_CAEEL Muscle M-line assembly protein unc-89... 29 4.5
sp|Q4A0V8|UAFA_STAS1 Uro-adherence factor A precursor 29 4.5
sp|P11654|PO210_RAT Nuclear pore membrane glycoprotein 210 ... 29 4.5
sp|P40357|SEC9_YEAST Protein transport protein SEC9 29 5.8
>sp|P51534|SHE4_YEAST Protein SHE4
Length = 789
Score = 31.6 bits (70), Expect = 0.90
Identities = 19/59 (32%), Positives = 29/59 (49%)
Frame = +1
Query: 4 TWNATKNVVHSPTNVKSLAEIQVEESEKINQEKIPNDNEAVISTEKSSKKEPSISLKSY 180
TW+ TK N+K L+EI + + KI N NE+ + E+ K E S+ +Y
Sbjct: 316 TWSFTKLTC---INLKQLSEIFINAISRRIMPKIENVNESAVKLEEVPKVEMSVEALAY 371
>sp|P38041|BOB1_YEAST Protein BOB1 (BEM1-binding protein)
Length = 980
Score = 31.2 bits (69), Expect = 1.2
Identities = 20/67 (29%), Positives = 36/67 (53%), Gaps = 1/67 (1%)
Frame = +1
Query: 19 KNVVHSPTNVKSLAEIQVEESEKINQEKIPNDNEAVISTEKSSKKEPSISLKSYPVAQKT 198
+ V SPT V ++ Q+++ + + QEK N+ + SS + ++S +S K+
Sbjct: 144 QEVSKSPTRVPEVSTPQLQDEQTLIQEKTRNEENTTHDSLFSSTADLNLSSESLKNISKS 203
Query: 199 N-NTKSL 216
N +TKSL
Sbjct: 204 NISTKSL 210
>sp|O13759|CSX1_SCHPO RNA-binding post-transcriptional regulator csx1
Length = 632
Score = 31.2 bits (69), Expect = 1.2
Identities = 20/68 (29%), Positives = 35/68 (51%), Gaps = 5/68 (7%)
Frame = +1
Query: 31 HSPTNVKSLAEIQVEESEK-----INQEKIPNDNEAVISTEKSSKKEPSISLKSYPVAQK 195
H+ V +L++ Q + S++ ++ +I N++VI S SLKS PV+ +
Sbjct: 368 HNTLPVSALSQSQSQVSDEGFDRTLSANQIFGMNQSVIGANSGSSNSSGSSLKSAPVSPR 427
Query: 196 TNNTKSLL 219
T +SLL
Sbjct: 428 TAAAQSLL 435
>sp|O43493|TGON2_HUMAN Trans-Golgi network integral membrane protein 2 precursor
(Trans-Golgi network protein TGN51) (TGN46) (TGN48)
(TGN38 homolog)
Length = 480
Score = 30.0 bits (66), Expect = 2.6
Identities = 36/140 (25%), Positives = 60/140 (42%), Gaps = 4/140 (2%)
Frame = +1
Query: 43 NVKSLAEIQVEESEKINQEKIP-NDNEAVISTEKSSKKEPSISLKSYPVAQ--KTNNTKS 213
NV + + + +E + QE+ + +ST S + P S KS+P Q K + +KS
Sbjct: 11 NVAAAGAVPLLATESVKQEEAGVRPSAGNVSTHPSLSQRPGGSTKSHPEPQTPKDSPSKS 70
Query: 214 LLEIQXXXXXXXXXXXXLENISKINETSTLNNPDKAETPV-SWKKTPLAVTTSVPSTTKS 390
E Q +K + S+ + +A+TP S K+ T+ ST+KS
Sbjct: 71 SAEAQTPEDTPNKSGGE----AKTLKDSSNKSGAEAQTPKGSTSKSGSEAQTTKDSTSKS 126
Query: 391 LLEIQAEEALKEKRYKELRT 450
E+Q + K E +T
Sbjct: 127 HPELQTPKDSTGKSGAEAQT 146
>sp|P25386|USO1_YEAST Intracellular protein transport protein USO1 (Int-1)
Length = 1790
Score = 29.3 bits (64), Expect = 4.5
Identities = 35/134 (26%), Positives = 53/134 (39%)
Frame = +1
Query: 61 EIQVEESEKINQEKIPNDNEAVISTEKSSKKEPSISLKSYPVAQKTNNTKSLLEIQXXXX 240
E Q+ E+ E+I N+ + ST++ ++ SI K+ + + KS E Q
Sbjct: 1165 EEQIANKERQYNEEISQLNDEITSTQQENE---SIKKKNDELEGEVKAMKSTSEEQSNLK 1221
Query: 241 XXXXXXXXLENISKINETSTLNNPDKAETPVSWKKTPLAVTTSVPSTTKSLLEIQAEEAL 420
L+ I E N ++A S K SV S T + E+Q E
Sbjct: 1222 KSEIDALNLQ----IKELKKKNETNEASLLESIK--------SVESETVKIKELQDECNF 1269
Query: 421 KEKRYKELRTIAKS 462
KEK EL K+
Sbjct: 1270 KEKEVSELEDKLKA 1283
>sp|P12036|NFH_HUMAN Neurofilament triplet H protein (200 kDa neurofilament protein)
(Neurofilament heavy polypeptide) (NF-H)
Length = 1026
Score = 29.3 bits (64), Expect = 4.5
Identities = 30/145 (20%), Positives = 57/145 (39%)
Frame = +1
Query: 19 KNVVHSPTNVKSLAEIQVEESEKINQEKIPNDNEAVISTEKSSKKEPSISLKSYPVAQKT 198
K SP VKS + + E+ + E S EK K P KS A+
Sbjct: 623 KEEAKSPAEVKSPEKAKSPTKEEAKSPEKAKSPEKAKSPEKEEAKSPE-KAKSPVKAEAK 681
Query: 199 NNTKSLLEIQXXXXXXXXXXXXLENISKINETSTLNNPDKAETPVSWKKTPLAVTTSVPS 378
+ K+ ++ ++ +K E + ++A++P K+P+ P
Sbjct: 682 SPEKAKSPVKAEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEK-AKSPVKEEAKTPE 740
Query: 379 TTKSLLEIQAEEALKEKRYKELRTI 453
KS ++ +A+ K K ++ +T+
Sbjct: 741 KAKSPVKEEAKSPEKAKSPEKAKTL 765
>sp|O01761|UNC89_CAEEL Muscle M-line assembly protein unc-89 (Uncoordinated protein 89)
Length = 8081
Score = 29.3 bits (64), Expect = 4.5
Identities = 31/126 (24%), Positives = 50/126 (39%), Gaps = 2/126 (1%)
Frame = +1
Query: 16 TKNVVHSPTNVKSLAEIQVEESEKINQEKIPN--DNEAVISTEKSSKKEPSISLKSYPVA 189
++N V SPT + E V E K +EK P D++ T+K E S + V
Sbjct: 1594 SENEVKSPTKKEKSPEKSVVEELKSPKEKSPEKADDKPKSPTKKEKSPEKS---ATEDVK 1650
Query: 190 QKTNNTKSLLEIQXXXXXXXXXXXXLENISKINETSTLNNPDKAETPVSWKKTPLAVTTS 369
T KS +++ + +K + T D+ ++P +K+P V
Sbjct: 1651 SPTKKEKSPEKVEEKP----------TSPTKKESSPTKKTDDEVKSPTKKEKSPQTVEEK 1700
Query: 370 VPSTTK 387
S TK
Sbjct: 1701 PASPTK 1706
Score = 28.1 bits (61), Expect = 9.9
Identities = 34/135 (25%), Positives = 54/135 (40%), Gaps = 3/135 (2%)
Frame = +1
Query: 34 SPTNVKSLAEIQVEESEK--INQEKIPNDNEAVISTEKSSKKEPSISLKSYPVAQKTNNT 207
SPT E + E K + +EK P E +KKE S + P + N
Sbjct: 1541 SPTKKDKSPEKSITEEIKSPVKKEKSPEKVEE--KPASPTKKEKSPEKPASPTKKSENEV 1598
Query: 208 KSLLEIQXXXXXXXXXXXXLENISKINETSTLNNPDKAETPVSWKKTP-LAVTTSVPSTT 384
KS + + +E + E S DK ++P +K+P + T V S T
Sbjct: 1599 KSPTKKE-----KSPEKSVVEELKSPKEKSPEKADDKPKSPTKKEKSPEKSATEDVKSPT 1653
Query: 385 KSLLEIQAEEALKEK 429
K + ++ E ++EK
Sbjct: 1654 K---KEKSPEKVEEK 1665
>sp|Q4A0V8|UAFA_STAS1 Uro-adherence factor A precursor
Length = 2316
Score = 29.3 bits (64), Expect = 4.5
Identities = 28/130 (21%), Positives = 61/130 (46%), Gaps = 4/130 (3%)
Frame = +1
Query: 49 KSLAEIQ-VEESEKINQEKIPNDNEAVISTEKSSKKEPSISLKSYPVAQKTNNTKSLLEI 225
+SL+E + + ESE ++ + +D+E++ ++E S E + +S ++ + ++SL E
Sbjct: 953 ESLSESESLSESESLSASESLSDSESLSASESLSASESLSASESLSASESLSASESLSES 1012
Query: 226 QXXXXXXXXXXXXLENISKINETSTLNNPDKAETPVSWKKTPLAVTTSVPSTTKSLLE-- 399
+ L ++E+ +L+ + S + T+ ST++SL E
Sbjct: 1013 E-----SLSDSESLSESESLSESESLSTSESLSASESISASESLSTSESLSTSESLSESE 1067
Query: 400 -IQAEEALKE 426
+ E+L E
Sbjct: 1068 SLSESESLSE 1077
>sp|P11654|PO210_RAT Nuclear pore membrane glycoprotein 210 precursor (Nuclear pore
membrane glycoprotein 210)
Length = 1886
Score = 29.3 bits (64), Expect = 4.5
Identities = 15/33 (45%), Positives = 22/33 (66%)
Frame = +1
Query: 313 DKAETPVSWKKTPLAVTTSVPSTTKSLLEIQAE 411
DK ++ KKT LAVT S+PS+ S+ ++ AE
Sbjct: 1652 DKQLKHLNMKKTSLAVTASMPSSRTSVEKVGAE 1684
>sp|P40357|SEC9_YEAST Protein transport protein SEC9
Length = 651
Score = 28.9 bits (63), Expect = 5.8
Identities = 39/152 (25%), Positives = 61/152 (40%), Gaps = 7/152 (4%)
Frame = +1
Query: 7 WNATKNVVHSPTNVKSLAEIQVEESEKINQEK---IPNDNEAVISTEKSSKKEPSISLKS 177
+NAT N K+ EIQ EE + QE+ + + + T++SS +LK
Sbjct: 391 FNATNNQYGDQRGYKTFEEIQKEEEARQQQEEDEAVDEIKQEIKFTKQSSVASTRNTLKM 450
Query: 178 YPVAQKTN-NTKSLLEIQXXXXXXXXXXXXLENISKINETSTLNNPDKAETPVSWKK--- 345
A++ NT +L Q L N+ + + N E KK
Sbjct: 451 AQDAERAGMNTLGMLGHQ---------SEQLNNVEGNLDLMKVQNKVADEKVAELKKLNR 501
Query: 346 TPLAVTTSVPSTTKSLLEIQAEEALKEKRYKE 441
+ LAV S P +K + EE LK ++ +E
Sbjct: 502 SILAVHVSNPFNSKRRRR-EREEQLKNRKIEE 532
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.298 0.115 0.294
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 40,314,193
Number of Sequences: 369166
Number of extensions: 574530
Number of successful extensions: 1233
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1203
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1232
length of database: 68,354,980
effective HSP length: 101
effective length of database: 49,696,745
effective search space used: 2584230740
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (21.6 bits)