Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Dr_sW_023_P06 (417 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|P13086|SUCA_RAT Succinyl-CoA ligase [GDP-forming] alpha-... 118 6e-27 sp|Q94522|SUCA_DROME Succinyl-CoA ligase [GDP-forming] alph... 117 9e-27 sp|Q9WUM5|SUCA_MOUSE Succinyl-CoA ligase [GDP-forming] alph... 116 2e-26 sp|P53597|SUCA_HUMAN Succinyl-CoA ligase [GDP-forming] alph... 116 2e-26 sp|P53596|SUCA_CAEEL Probable succinyl-CoA ligase [GDP-form... 113 1e-25 sp|P36967|SUCA_DICDI Succinyl-CoA ligase [GDP-forming] alph... 112 2e-25 sp|Q8LAD2|SUCA2_ARATH Succinyl-CoA ligase [GDP-forming] alp... 109 3e-24 sp|P68209|SUCA1_ARATH Succinyl-CoA ligase [GDP-forming] alp... 109 3e-24 sp|O13750|SUCA_SCHPO Probable succinyl-CoA ligase [GDP-form... 106 2e-23 sp|O08371|SUCD_RICPR Succinyl-CoA synthetase alpha chain (S... 104 8e-23
>sp|P13086|SUCA_RAT Succinyl-CoA ligase [GDP-forming] alpha-chain, mitochondrial precursor (Succinyl-CoA synthetase, alpha chain) (SCS-alpha) Length = 333 Score = 118 bits (295), Expect = 6e-27 Identities = 54/66 (81%), Positives = 61/66 (92%) Frame = +3 Query: 3 IMPAHIHKPGKIGIVSRSGTLTYEAVHQTSQVGLGQSLVIGIGGDPFNGTNFVDCLEVFV 182 IMP HIHK G+IGIVSRSGTLTYEAVHQT+QVGLGQSL IGIGGDPFNGTNF+DCL+VF+ Sbjct: 172 IMPGHIHKKGRIGIVSRSGTLTYEAVHQTTQVGLGQSLCIGIGGDPFNGTNFIDCLDVFL 231 Query: 183 RDEHTK 200 +D T+ Sbjct: 232 KDPATE 237
Score = 35.4 bits (80), Expect = 0.047 Identities = 18/26 (69%), Positives = 21/26 (80%), Gaps = 1/26 (3%) Frame = +1 Query: 280 KSRAVVSFIAGLTAPPDAD-GHAGAI 354 K++ VVSFIAG+TAPP GHAGAI Sbjct: 265 KAKPVVSFIAGITAPPGRRMGHAGAI 290
>sp|Q94522|SUCA_DROME Succinyl-CoA ligase [GDP-forming] alpha-chain, mitochondrial precursor (Succinyl-CoA synthetase, alpha chain) (SCS-alpha) Length = 328 Score = 117 bits (293), Expect = 9e-27 Identities = 52/66 (78%), Positives = 61/66 (92%) Frame = +3 Query: 3 IMPAHIHKPGKIGIVSRSGTLTYEAVHQTSQVGLGQSLVIGIGGDPFNGTNFVDCLEVFV 182 IMP HIHK GKIG+VSRSGTLTYEAVHQT++VGLGQ+L +GIGGDPFNGT+F+DCLEVF+ Sbjct: 167 IMPGHIHKRGKIGVVSRSGTLTYEAVHQTTEVGLGQTLCVGIGGDPFNGTDFIDCLEVFL 226 Query: 183 RDEHTK 200 +D TK Sbjct: 227 KDPETK 232
Score = 33.5 bits (75), Expect = 0.18 Identities = 17/26 (65%), Positives = 21/26 (80%), Gaps = 1/26 (3%) Frame = +1 Query: 280 KSRAVVSFIAGLTAPPDAD-GHAGAI 354 K++ VVSFIAG++APP GHAGAI Sbjct: 260 KAKPVVSFIAGVSAPPGRRMGHAGAI 285
>sp|Q9WUM5|SUCA_MOUSE Succinyl-CoA ligase [GDP-forming] alpha-chain, mitochondrial precursor (Succinyl-CoA synthetase, alpha chain) (SCS-alpha) Length = 333 Score = 116 bits (291), Expect = 2e-26 Identities = 54/66 (81%), Positives = 60/66 (90%) Frame = +3 Query: 3 IMPAHIHKPGKIGIVSRSGTLTYEAVHQTSQVGLGQSLVIGIGGDPFNGTNFVDCLEVFV 182 IMP HIHK G+IGIVSRSGTLTYEAVHQT+QVGLGQSL IGIGGDPFNGT+F+DCLEVF+ Sbjct: 172 IMPGHIHKKGRIGIVSRSGTLTYEAVHQTTQVGLGQSLCIGIGGDPFNGTDFIDCLEVFL 231 Query: 183 RDEHTK 200 D T+ Sbjct: 232 NDPATE 237
Score = 35.4 bits (80), Expect = 0.047 Identities = 18/26 (69%), Positives = 21/26 (80%), Gaps = 1/26 (3%) Frame = +1 Query: 280 KSRAVVSFIAGLTAPPDAD-GHAGAI 354 K++ VVSFIAG+TAPP GHAGAI Sbjct: 265 KAKPVVSFIAGITAPPGRRMGHAGAI 290
>sp|P53597|SUCA_HUMAN Succinyl-CoA ligase [GDP-forming] alpha-chain, mitochondrial precursor (Succinyl-CoA synthetase, alpha chain) (SCS-alpha) Length = 333 Score = 116 bits (290), Expect = 2e-26 Identities = 52/66 (78%), Positives = 60/66 (90%) Frame = +3 Query: 3 IMPAHIHKPGKIGIVSRSGTLTYEAVHQTSQVGLGQSLVIGIGGDPFNGTNFVDCLEVFV 182 IMP HIHK G+IGIVSRSGTLTYEAVHQT+QVGLGQSL +GIGGDPFNGT+F+DCLE+F+ Sbjct: 172 IMPGHIHKKGRIGIVSRSGTLTYEAVHQTTQVGLGQSLCVGIGGDPFNGTDFIDCLEIFL 231 Query: 183 RDEHTK 200 D T+ Sbjct: 232 NDSATE 237
Score = 35.4 bits (80), Expect = 0.047 Identities = 19/25 (76%), Positives = 20/25 (80%), Gaps = 1/25 (4%) Frame = +1 Query: 283 SRAVVSFIAGLTAPPDAD-GHAGAI 354 S+ VVSFIAGLTAPP GHAGAI Sbjct: 266 SKPVVSFIAGLTAPPGRRMGHAGAI 290
>sp|P53596|SUCA_CAEEL Probable succinyl-CoA ligase [GDP-forming] alpha-chain, mitochondrial precursor (Succinyl-CoA synthetase, alpha chain) (SCS-alpha) Length = 322 Score = 113 bits (283), Expect = 1e-25 Identities = 52/66 (78%), Positives = 57/66 (86%) Frame = +3 Query: 3 IMPAHIHKPGKIGIVSRSGTLTYEAVHQTSQVGLGQSLVIGIGGDPFNGTNFVDCLEVFV 182 IMP HIHK G IGIVSRSGTLTYEAVHQT+QVG GQ+L +GIGGDPFNGTNF+DCL VF+ Sbjct: 163 IMPGHIHKRGCIGIVSRSGTLTYEAVHQTTQVGFGQTLCVGIGGDPFNGTNFIDCLNVFL 222 Query: 183 RDEHTK 200 D TK Sbjct: 223 EDPETK 228
Score = 32.7 bits (73), Expect = 0.31 Identities = 17/24 (70%), Positives = 19/24 (79%), Gaps = 1/24 (4%) Frame = +1 Query: 286 RAVVSFIAGLTAPPDAD-GHAGAI 354 + VVSFIAG+TAPP GHAGAI Sbjct: 258 KPVVSFIAGVTAPPGRRMGHAGAI 281
>sp|P36967|SUCA_DICDI Succinyl-CoA ligase [GDP-forming] alpha-chain, mitochondrial precursor (Succinyl-CoA synthetase, alpha chain) (SCS-alpha) Length = 310 Score = 112 bits (281), Expect = 2e-25 Identities = 52/66 (78%), Positives = 58/66 (87%) Frame = +3 Query: 3 IMPAHIHKPGKIGIVSRSGTLTYEAVHQTSQVGLGQSLVIGIGGDPFNGTNFVDCLEVFV 182 IMP HIHKPGKIGIVSRSGTLTYEAV QT+ VGLGQS IGIGGDPFNGTNF+DCL++F Sbjct: 157 IMPGHIHKPGKIGIVSRSGTLTYEAVAQTTAVGLGQSTCIGIGGDPFNGTNFIDCLKMFT 216 Query: 183 RDEHTK 200 +D T+ Sbjct: 217 QDPQTE 222
Score = 32.3 bits (72), Expect = 0.40 Identities = 17/24 (70%), Positives = 19/24 (79%), Gaps = 1/24 (4%) Frame = +1 Query: 286 RAVVSFIAGLTAPPDAD-GHAGAI 354 + VVSFIAGL+APP GHAGAI Sbjct: 249 KPVVSFIAGLSAPPGRRMGHAGAI 272
>sp|Q8LAD2|SUCA2_ARATH Succinyl-CoA ligase [GDP-forming] alpha-chain 2, mitochondrial precursor (Succinyl-CoA synthetase 2, alpha chain) (SCS2-alpha) Length = 341 Score = 109 bits (272), Expect = 3e-24 Identities = 52/66 (78%), Positives = 56/66 (84%) Frame = +3 Query: 3 IMPAHIHKPGKIGIVSRSGTLTYEAVHQTSQVGLGQSLVIGIGGDPFNGTNFVDCLEVFV 182 IMP +IHKPGKIGIVSRSGTLTYEAV QT+ VGLGQS +GIGGDPFNGTNFVDCLE F Sbjct: 182 IMPGYIHKPGKIGIVSRSGTLTYEAVFQTTAVGLGQSTCVGIGGDPFNGTNFVDCLEKFF 241 Query: 183 RDEHTK 200 D T+ Sbjct: 242 VDPQTE 247
Score = 32.7 bits (73), Expect = 0.31 Identities = 17/24 (70%), Positives = 19/24 (79%), Gaps = 1/24 (4%) Frame = +1 Query: 286 RAVVSFIAGLTAPPDAD-GHAGAI 354 + VV+FIAGLTAPP GHAGAI Sbjct: 274 KPVVAFIAGLTAPPGRRMGHAGAI 297
>sp|P68209|SUCA1_ARATH Succinyl-CoA ligase [GDP-forming] alpha-chain 1, mitochondrial precursor (Succinyl-CoA synthetase 1, alpha chain) (SCS1-alpha) Length = 347 Score = 109 bits (272), Expect = 3e-24 Identities = 52/66 (78%), Positives = 56/66 (84%) Frame = +3 Query: 3 IMPAHIHKPGKIGIVSRSGTLTYEAVHQTSQVGLGQSLVIGIGGDPFNGTNFVDCLEVFV 182 IMP +IHKPGKIGIVSRSGTLTYEAV QT+ VGLGQS +GIGGDPFNGTNFVDCLE F Sbjct: 187 IMPGYIHKPGKIGIVSRSGTLTYEAVFQTTAVGLGQSTCVGIGGDPFNGTNFVDCLEKFF 246 Query: 183 RDEHTK 200 D T+ Sbjct: 247 VDPQTE 252
Score = 32.7 bits (73), Expect = 0.31 Identities = 17/24 (70%), Positives = 19/24 (79%), Gaps = 1/24 (4%) Frame = +1 Query: 286 RAVVSFIAGLTAPPDAD-GHAGAI 354 + VV+FIAGLTAPP GHAGAI Sbjct: 279 KPVVAFIAGLTAPPGRRMGHAGAI 302
>sp|O13750|SUCA_SCHPO Probable succinyl-CoA ligase [GDP-forming] alpha-chain, mitochondrial precursor (Succinyl-CoA synthetase, alpha chain) (SCS-alpha) Length = 331 Score = 106 bits (264), Expect = 2e-23 Identities = 50/66 (75%), Positives = 59/66 (89%) Frame = +3 Query: 3 IMPAHIHKPGKIGIVSRSGTLTYEAVHQTSQVGLGQSLVIGIGGDPFNGTNFVDCLEVFV 182 IMP+HIHKPG IGIVSRSGTLTYEAV+QT+Q LGQSLVIGIGGDPF GTNF+D L++F+ Sbjct: 170 IMPSHIHKPGCIGIVSRSGTLTYEAVNQTTQTDLGQSLVIGIGGDPFPGTNFIDALKLFL 229 Query: 183 RDEHTK 200 D +T+ Sbjct: 230 DDPNTQ 235
Score = 31.6 bits (70), Expect = 0.68 Identities = 18/28 (64%), Positives = 19/28 (67%), Gaps = 1/28 (3%) Frame = +1 Query: 274 RSKSRAVVSFIAGLTAPPDAD-GHAGAI 354 RS + VVSFIAG TAP GHAGAI Sbjct: 262 RSTPKPVVSFIAGATAPKGRRMGHAGAI 289
>sp|O08371|SUCD_RICPR Succinyl-CoA synthetase alpha chain (SCS-alpha) Length = 292 Score = 104 bits (259), Expect = 8e-23 Identities = 48/66 (72%), Positives = 56/66 (84%) Frame = +3 Query: 3 IMPAHIHKPGKIGIVSRSGTLTYEAVHQTSQVGLGQSLVIGIGGDPFNGTNFVDCLEVFV 182 IMP HIHK G IGIVSRSGTLTYEAV QT+ GLGQS +GIGGDP NGT+FVDC+E+F+ Sbjct: 137 IMPGHIHKIGDIGIVSRSGTLTYEAVAQTTAAGLGQSTCVGIGGDPVNGTSFVDCIEMFL 196 Query: 183 RDEHTK 200 +D+ TK Sbjct: 197 QDDETK 202
Score = 32.3 bits (72), Expect = 0.40 Identities = 16/24 (66%), Positives = 19/24 (79%), Gaps = 1/24 (4%) Frame = +1 Query: 286 RAVVSFIAGLTAPPDAD-GHAGAI 354 + +VSFIAG+TAP D GHAGAI Sbjct: 229 KPIVSFIAGITAPADKRMGHAGAI 252
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 48,259,870 Number of Sequences: 369166 Number of extensions: 842899 Number of successful extensions: 2080 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 2024 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 2077 length of database: 68,354,980 effective HSP length: 99 effective length of database: 50,066,215 effective search space used: 1952582385 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)