Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_023_N24
(562 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O43852|CALU_HUMAN Calumenin precursor (Crocalbin) (IEF S... 208 6e-54
sp|O35887|CALU_MOUSE Calumenin precursor (Crocalbin) 208 6e-54
sp|O35783|CALU_RAT Calumenin precursor (Crocalbin) (CBP-50) 204 2e-52
sp|O93434|RCN1_FUGRU Reticulocalbin-1 precursor 190 2e-48
sp|Q15293|RCN1_HUMAN Reticulocalbin-1 precursor 189 3e-48
sp|Q05186|RCN1_MOUSE Reticulocalbin-1 precursor 187 1e-47
sp|Q8BH97|RCN3_MOUSE Reticulocalbin-3 precursor 186 3e-47
sp|Q96D15|RCN3_HUMAN Reticulocalbin-3 precursor (EF-hand ca... 182 6e-46
sp|Q14257|RCN2_HUMAN Reticulocalbin-2 precursor (Calcium-bi... 124 2e-28
sp|Q8BP92|RCN2_MOUSE Reticulocalbin-2 precursor (Taipoxin-a... 124 2e-28
>sp|O43852|CALU_HUMAN Calumenin precursor (Crocalbin) (IEF SSP 9302)
Length = 315
Score = 208 bits (530), Expect = 6e-54
Identities = 95/157 (60%), Positives = 124/157 (78%)
Frame = +1
Query: 1 RRWEKADTDGDKLLSKAEFFDFLHPEESEKMRDVVIQETLDDIDKNKDGVIDLDEYIHDM 180
RR++ AD DGD + +K EF FLHPEE + M+D+V+QET++DIDKN DG IDL+EYI DM
Sbjct: 158 RRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDM 217
Query: 181 WTPEKNEEEPDWVKTERTQFIEHRDLNKDGKLDHDEIGKWILPLDHNHVTAETNHLMEQT 360
++ + N +EP+WVKTER QF+E RD N+DGK+D +E WILP D++H AE HL+ ++
Sbjct: 218 YSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYES 277
Query: 361 DANKDGYLTKVEILEKYDVFVGSQATDFGDALTRHAE 471
D NKDG LTK EI++KYD+FVGSQATDFG+AL RH E
Sbjct: 278 DQNKDGKLTKEEIVDKYDLFVGSQATDFGEALVRHDE 314
Score = 30.8 bits (68), Expect = 2.4
Identities = 38/173 (21%), Positives = 71/173 (41%), Gaps = 45/173 (26%)
Frame = +1
Query: 19 DTDGDKLLS--KAEFFDFLHPEESEKMRDVVIQE---------TLDDI------------ 129
D D D L +A+ FD L PEES++ ++ + T+D++
Sbjct: 46 DYDHDAFLGAEEAKTFDQLTPEESKERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWI 105
Query: 130 -----------DKNKDGVIDLDEYIHDMWTPEKNEEEPD--------WVKTERTQFIEHR 252
D N+DG++ +EY + + ++ +PD V+ ER +
Sbjct: 106 YEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDER----RFK 161
Query: 253 DLNKDGKL--DHDEIGKWILPLDHNHV-TAETNHLMEQTDANKDGYLTKVEIL 402
+KDG L +E ++ P +++++ ME D N DG++ E +
Sbjct: 162 MADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYI 214
>sp|O35887|CALU_MOUSE Calumenin precursor (Crocalbin)
Length = 315
Score = 208 bits (530), Expect = 6e-54
Identities = 95/157 (60%), Positives = 124/157 (78%)
Frame = +1
Query: 1 RRWEKADTDGDKLLSKAEFFDFLHPEESEKMRDVVIQETLDDIDKNKDGVIDLDEYIHDM 180
RR++ AD DGD + +K EF FLHPEE + M+D+V+QET++DIDKN DG IDL+EYI DM
Sbjct: 158 RRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDM 217
Query: 181 WTPEKNEEEPDWVKTERTQFIEHRDLNKDGKLDHDEIGKWILPLDHNHVTAETNHLMEQT 360
++ + N +EP+WVKTER QF+E RD N+DGK+D +E WILP D++H AE HL+ ++
Sbjct: 218 YSHDGNADEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYES 277
Query: 361 DANKDGYLTKVEILEKYDVFVGSQATDFGDALTRHAE 471
D NKDG LTK EI++KYD+FVGSQATDFG+AL RH E
Sbjct: 278 DQNKDGKLTKEEIVDKYDLFVGSQATDFGEALVRHDE 314
Score = 31.2 bits (69), Expect = 1.8
Identities = 43/190 (22%), Positives = 79/190 (41%), Gaps = 45/190 (23%)
Frame = +1
Query: 19 DTDGDKLLS--KAEFFDFLHPEESEKMRDVVIQE---------TLDDI------------ 129
D D D L +A+ FD L PEES++ ++ + T+D++
Sbjct: 46 DYDHDAFLGAEEAKSFDQLTPEESKERLGKIVSKIDDDKDGFVTVDELKGWIKFAQKRWI 105
Query: 130 -----------DKNKDGVIDLDEYIHDMWTPEKNEEEPD--------WVKTERTQFIEHR 252
D N+DG++ +EY + + ++ +PD V+ ER +
Sbjct: 106 HEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDER----RFK 161
Query: 253 DLNKDGKL--DHDEIGKWILPLDHNHV-TAETNHLMEQTDANKDGYLTKVEILEKYDVFV 423
+KDG L +E ++ P +++++ ME D N DG++ LE+Y +
Sbjct: 162 MADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFID----LEEY---I 214
Query: 424 GSQATDFGDA 453
G + G+A
Sbjct: 215 GDMYSHDGNA 224
>sp|O35783|CALU_RAT Calumenin precursor (Crocalbin) (CBP-50)
Length = 315
Score = 204 bits (518), Expect = 2e-52
Identities = 93/157 (59%), Positives = 123/157 (78%)
Frame = +1
Query: 1 RRWEKADTDGDKLLSKAEFFDFLHPEESEKMRDVVIQETLDDIDKNKDGVIDLDEYIHDM 180
RR++ AD DGD + +K EF FLHPEE + M+D+V+QET++DID+N DG IDL+EYI DM
Sbjct: 158 RRFKMADQDGDLIATKEEFTAFLHPEEYDYMKDIVLQETMEDIDQNADGFIDLEEYIGDM 217
Query: 181 WTPEKNEEEPDWVKTERTQFIEHRDLNKDGKLDHDEIGKWILPLDHNHVTAETNHLMEQT 360
++ + N +EP WVKTER QF+E RD N+DGK+D +E WILP D++H AE HL+ ++
Sbjct: 218 YSHDGNADEPQWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYES 277
Query: 361 DANKDGYLTKVEILEKYDVFVGSQATDFGDALTRHAE 471
D +KDG LTK EI++KYD+FVGSQATDFG+AL RH E
Sbjct: 278 DQDKDGKLTKEEIVDKYDLFVGSQATDFGEALVRHDE 314
>sp|O93434|RCN1_FUGRU Reticulocalbin-1 precursor
Length = 322
Score = 190 bits (482), Expect = 2e-48
Identities = 91/159 (57%), Positives = 119/159 (74%), Gaps = 1/159 (0%)
Frame = +1
Query: 1 RRWEKADTDGDKLLSKAEFFDFLHPEESEKMRDVVIQETLDDIDKNKDGVIDLDEYIHDM 180
RR+++AD DGD ++ EF FLHPEE E M+D+V+ ETL+DIDKN DG +D DEYI DM
Sbjct: 164 RRFKRADLDGDSAANREEFTSFLHPEEFEHMKDIVVLETLEDIDKNSDGHVDEDEYIADM 223
Query: 181 WTPEKNEEEPDWVKTERTQFIEHRDLNKDGKLDHDEIGKWILPLDHNHVTAETNHLMEQT 360
+ E EP+WVKTER QF + RDLNKDGK+D DEI WI+P D++H AE HL+ ++
Sbjct: 224 FAHEDRGPEPEWVKTEREQFSDFRDLNKDGKMDLDEIRHWIMPQDYDHAQAEARHLVYES 283
Query: 361 DANKDGYLTKVEILEKYDVFVGSQATDFGDALTR-HAEL 474
D +KD LTK EIL+ +++FVGSQAT++G+ LTR H EL
Sbjct: 284 DKDKDQMLTKEEILDNWNMFVGSQATNYGEDLTRNHDEL 322
>sp|Q15293|RCN1_HUMAN Reticulocalbin-1 precursor
Length = 331
Score = 189 bits (481), Expect = 3e-48
Identities = 93/159 (58%), Positives = 120/159 (75%), Gaps = 1/159 (0%)
Frame = +1
Query: 1 RRWEKADTDGDKLLSKAEFFDFLHPEESEKMRDVVIQETLDDIDKNKDGVIDLDEYIHDM 180
RR++ AD +GD ++ EF FLHPEE E M+++V+ ETL+DIDKN DG +D DEYI DM
Sbjct: 173 RRFKAADLNGDLTATREEFTAFLHPEEFEHMKEIVVLETLEDIDKNGDGFVDQDEYIADM 232
Query: 181 WTPEKNEEEPDWVKTERTQFIEHRDLNKDGKLDHDEIGKWILPLDHNHVTAETNHLMEQT 360
++ E+N EPDWV +ER QF E RDLNKDGKLD DEI WILP D++H AE HL+ ++
Sbjct: 233 FSHEENGPEPDWVLSEREQFNEFRDLNKDGKLDKDEIRHWILPQDYDHAQAEARHLVYES 292
Query: 361 DANKDGYLTKVEILEKYDVFVGSQATDFGDALTR-HAEL 474
D NKD LTK EILE +++FVGSQAT++G+ LT+ H EL
Sbjct: 293 DKNKDEKLTKEEILENWNMFVGSQATNYGEDLTKNHDEL 331
>sp|Q05186|RCN1_MOUSE Reticulocalbin-1 precursor
Length = 325
Score = 187 bits (476), Expect = 1e-47
Identities = 91/159 (57%), Positives = 119/159 (74%), Gaps = 1/159 (0%)
Frame = +1
Query: 1 RRWEKADTDGDKLLSKAEFFDFLHPEESEKMRDVVIQETLDDIDKNKDGVIDLDEYIHDM 180
RR++ +D DGD ++ EF FLHPEE E M+++V+ ETL+DIDKN DG +D DEYI DM
Sbjct: 167 RRFKASDLDGDLTATREEFTAFLHPEEFEHMKEIVVLETLEDIDKNGDGFVDQDEYIADM 226
Query: 181 WTPEKNEEEPDWVKTERTQFIEHRDLNKDGKLDHDEIGKWILPLDHNHVTAETNHLMEQT 360
++ E N EPDWV +ER QF + RDLNKDGKLD DEI WILP D++H AE HL+ ++
Sbjct: 227 FSHEDNGPEPDWVLSEREQFNDFRDLNKDGKLDKDEIRHWILPQDYDHAQAEARHLVYES 286
Query: 361 DANKDGYLTKVEILEKYDVFVGSQATDFGDALTR-HAEL 474
D NKD LTK EIL+ +++FVGSQAT++G+ LT+ H EL
Sbjct: 287 DKNKDEMLTKEEILDNWNMFVGSQATNYGEDLTKNHDEL 325
Score = 28.9 bits (63), Expect = 9.1
Identities = 21/107 (19%), Positives = 49/107 (45%), Gaps = 6/107 (5%)
Frame = +1
Query: 163 EYIHDMWTPEKNEE-----EPDWVKTERTQFIEHRDLNKDGKLDHDEIGKWILPLDHNHV 327
+Y H+ + +++ + PD K + ++ D + DG + +E+ WI + ++
Sbjct: 51 QYDHEAFLGKEDSKTFDQLSPDESKERLGKIVDRIDSDGDGLVTTEELKLWIKRVQKRYI 110
Query: 328 TAETNHLMEQTDANKDGYLTKVEILE-KYDVFVGSQATDFGDALTRH 465
+ + D +KD ++ E + Y ++G+ A +F D+ H
Sbjct: 111 YDNVAKVWKDYDRDKDEKISWEEYKQATYGYYLGNPA-EFHDSSDHH 156
>sp|Q8BH97|RCN3_MOUSE Reticulocalbin-3 precursor
Length = 328
Score = 186 bits (472), Expect = 3e-47
Identities = 84/155 (54%), Positives = 114/155 (73%)
Frame = +1
Query: 1 RRWEKADTDGDKLLSKAEFFDFLHPEESEKMRDVVIQETLDDIDKNKDGVIDLDEYIHDM 180
RR+ AD DGD + ++ E FLHPEE MRD+V+ ETL+D+DKNKDG + ++EYI D+
Sbjct: 170 RRFRVADQDGDSMATREELTAFLHPEEFPHMRDIVVAETLEDLDKNKDGYVQVEEYIADL 229
Query: 181 WTPEKNEEEPDWVKTERTQFIEHRDLNKDGKLDHDEIGKWILPLDHNHVTAETNHLMEQT 360
++ E EEEP WV+TER QF E RDLNKDG+LD E+G W+LP + E NHL+ ++
Sbjct: 230 YSEEPGEEEPAWVQTERQQFREFRDLNKDGRLDGSEVGYWVLPPSQDQPLVEANHLLHES 289
Query: 361 DANKDGYLTKVEILEKYDVFVGSQATDFGDALTRH 465
D +KDG L+K EIL +++FVGSQAT++G+ LTRH
Sbjct: 290 DTDKDGRLSKAEILSNWNMFVGSQATNYGEDLTRH 324
>sp|Q96D15|RCN3_HUMAN Reticulocalbin-3 precursor (EF-hand calcium-binding protein RLP49)
Length = 328
Score = 182 bits (461), Expect = 6e-46
Identities = 83/155 (53%), Positives = 113/155 (72%)
Frame = +1
Query: 1 RRWEKADTDGDKLLSKAEFFDFLHPEESEKMRDVVIQETLDDIDKNKDGVIDLDEYIHDM 180
RR+ AD DGD + ++ E FLHPEE MRD+VI ETL+D+D+NKDG + ++EYI D+
Sbjct: 170 RRFRVADQDGDSMATREELTAFLHPEEFPHMRDIVIAETLEDLDRNKDGYVQVEEYIADL 229
Query: 181 WTPEKNEEEPDWVKTERTQFIEHRDLNKDGKLDHDEIGKWILPLDHNHVTAETNHLMEQT 360
++ E EEEP WV+TER QF + RDLNKDG LD E+G W+LP + E NHL+ ++
Sbjct: 230 YSAEPGEEEPAWVQTERQQFRDFRDLNKDGHLDGSEVGHWVLPPAQDQPLVEANHLLHES 289
Query: 361 DANKDGYLTKVEILEKYDVFVGSQATDFGDALTRH 465
D +KDG L+K EIL +++FVGSQAT++G+ LTRH
Sbjct: 290 DTDKDGRLSKAEILGNWNMFVGSQATNYGEDLTRH 324
>sp|Q14257|RCN2_HUMAN Reticulocalbin-2 precursor (Calcium-binding protein ERC-55)
(E6-binding protein) (E6BP)
Length = 317
Score = 124 bits (311), Expect = 2e-28
Identities = 63/153 (41%), Positives = 95/153 (62%), Gaps = 1/153 (0%)
Frame = +1
Query: 1 RRWEKADTDGDKLLSKAEFFDFLHPEESEKMRDVVIQETLDDIDKNKDGVIDLDEYIHD- 177
+R+EKA+ D LS EF F HPEE + M + VIQE L++ DKN DG + L+E++ D
Sbjct: 156 KRFEKANQDSGPGLSLEEFIAFEHPEEVDYMTEFVIQEALEEHDKNGDGFVSLEEFLGDY 215
Query: 178 MWTPEKNEEEPDWVKTERTQFIEHRDLNKDGKLDHDEIGKWILPLDHNHVTAETNHLMEQ 357
W P N E+P+W+ E+ +F+ D + DG+LD E+ W++P + E HL+++
Sbjct: 216 RWDPTAN-EDPEWILVEKDRFVNDYDKDNDGRLDPQELLPWVVPNNQGIAQEEALHLIDE 274
Query: 358 TDANKDGYLTKVEILEKYDVFVGSQATDFGDAL 456
D N D L++ EILE D+F+ S+ATD+G L
Sbjct: 275 MDLNGDKKLSEEEILENPDLFLTSEATDYGRQL 307
Score = 30.4 bits (67), Expect = 3.1
Identities = 33/146 (22%), Positives = 58/146 (39%), Gaps = 12/146 (8%)
Frame = +1
Query: 10 EKADTDGDKLLSKAEFFDFLHPEESEKMRDVVIQETLDDIDKNKDGVIDLDEYIHDMW-- 183
+K D D D L+++E ++ Q+ ++ DKN D + DEY M+
Sbjct: 71 KKIDLDSDGFLTESELSSWIQMSFKHYAMQEAKQQFVE-YDKNSDDTVTWDEYNIQMYDR 129
Query: 184 -------TPEKNEEEPDWVKTERTQFIEHRDLNKDG--KLDHDEIGKWILPLDHNHVTA- 333
T + EE + K N+D L +E + P + +++T
Sbjct: 130 VIDFDENTALDDAEEESFRKLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEVDYMTEF 189
Query: 334 ETNHLMEQTDANKDGYLTKVEILEKY 411
+E+ D N DG+++ E L Y
Sbjct: 190 VIQEALEEHDKNGDGFVSLEEFLGDY 215
Score = 30.0 bits (66), Expect = 4.1
Identities = 25/100 (25%), Positives = 44/100 (44%), Gaps = 1/100 (1%)
Frame = +1
Query: 154 DLDEYIHDMWTPEKNEEEPDWVKTERTQ-FIEHRDLNKDGKLDHDEIGKWILPLDHNHVT 330
D+DEY+ + EE + +R Q I+ DL+ DG L E+ WI ++
Sbjct: 50 DVDEYV------KLGHEE----QQKRLQAIIKKIDLDSDGFLTESELSSWIQMSFKHYAM 99
Query: 331 AETNHLMEQTDANKDGYLTKVEILEKYDVFVGSQATDFGD 450
E + D N D +T ++Y++ + + DF +
Sbjct: 100 QEAKQQFVEYDKNSDDTVT----WDEYNIQMYDRVIDFDE 135
>sp|Q8BP92|RCN2_MOUSE Reticulocalbin-2 precursor (Taipoxin-associated calcium-binding
protein 49) (TCBP-49)
Length = 320
Score = 124 bits (310), Expect = 2e-28
Identities = 60/152 (39%), Positives = 92/152 (60%)
Frame = +1
Query: 1 RRWEKADTDGDKLLSKAEFFDFLHPEESEKMRDVVIQETLDDIDKNKDGVIDLDEYIHDM 180
+R+EKA+ D LS EF F HPEE + M + VIQE L++ DKN DG + L+E++ D
Sbjct: 159 KRFEKANQDSGPGLSLEEFIAFEHPEEVDYMTEFVIQEALEEHDKNGDGFVSLEEFLGDY 218
Query: 181 WTPEKNEEEPDWVKTERTQFIEHRDLNKDGKLDHDEIGKWILPLDHNHVTAETNHLMEQT 360
E+P+W+ E+ +F+ D + DG+LD E+ W++P + E HL+++
Sbjct: 219 RRDPTANEDPEWILVEKDRFVNDYDKDNDGRLDPQELLSWVVPNNQGIAQEEALHLIDEM 278
Query: 361 DANKDGYLTKVEILEKYDVFVGSQATDFGDAL 456
D N D L++ EILE D+F+ S+ATD+G L
Sbjct: 279 DLNSDKKLSEEEILENQDLFLTSEATDYGRQL 310
Score = 30.8 bits (68), Expect = 2.4
Identities = 23/99 (23%), Positives = 43/99 (43%)
Frame = +1
Query: 154 DLDEYIHDMWTPEKNEEEPDWVKTERTQFIEHRDLNKDGKLDHDEIGKWILPLDHNHVTA 333
D+DEY+ + EE + I+ D + DG L +E+ +WI ++
Sbjct: 53 DVDEYV------KLGHEEQ---QRRLQSIIKKIDSDSDGFLTENELSQWIQMSFKHYAMQ 103
Query: 334 ETNHLMEQTDANKDGYLTKVEILEKYDVFVGSQATDFGD 450
E + D N DG +T ++Y++ + + DF +
Sbjct: 104 EAKQQFVEYDKNSDGAVT----WDEYNIQMYDRVIDFDE 138
Score = 30.8 bits (68), Expect = 2.4
Identities = 33/146 (22%), Positives = 59/146 (40%), Gaps = 12/146 (8%)
Frame = +1
Query: 10 EKADTDGDKLLSKAEFFDFLHPEESEKMRDVVIQETLDDIDKNKDGVIDLDEYIHDMW-- 183
+K D+D D L++ E ++ Q+ ++ DKN DG + DEY M+
Sbjct: 74 KKIDSDSDGFLTENELSQWIQMSFKHYAMQEAKQQFVE-YDKNSDGAVTWDEYNIQMYDR 132
Query: 184 -------TPEKNEEEPDWVKTERTQFIEHRDLNKDG--KLDHDEIGKWILPLDHNHVTA- 333
T + EE + + N+D L +E + P + +++T
Sbjct: 133 VIDFDENTALDDTEEGSFRQLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEVDYMTEF 192
Query: 334 ETNHLMEQTDANKDGYLTKVEILEKY 411
+E+ D N DG+++ E L Y
Sbjct: 193 VIQEALEEHDKNGDGFVSLEEFLGDY 218
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 73,278,675
Number of Sequences: 369166
Number of extensions: 1573793
Number of successful extensions: 5343
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4906
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5251
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4029688280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)