Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_023_M24
(394 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P05165|PCCA_HUMAN Propionyl-CoA carboxylase alpha chain,... 108 4e-24
sp|Q91ZA3|PCCA_MOUSE Propionyl-CoA carboxylase alpha chain,... 100 1e-21
sp|P14882|PCCA_RAT Propionyl-CoA carboxylase alpha chain, m... 99 4e-21
sp|Q05920|PYC_MOUSE Pyruvate carboxylase, mitochondrial pre... 68 6e-12
sp|P29337|BCCP_STRMU Biotin carboxyl carrier protein (BCCP) 68 6e-12
sp|P52873|PYC_RAT Pyruvate carboxylase, mitochondrial precu... 67 1e-11
sp|P11498|PYC_HUMAN Pyruvate carboxylase, mitochondrial pre... 67 1e-11
sp|Q9ZAA7|GCDC_ACIFE Glutaconyl-CoA decarboxylase gamma sub... 65 6e-11
sp|Q42777|MCCA_SOYBN Methylcrotonoyl-CoA carboxylase alpha ... 56 3e-08
sp|Q42523|MCCA_ARATH Methylcrotonoyl-CoA carboxylase alpha ... 55 4e-08
>sp|P05165|PCCA_HUMAN Propionyl-CoA carboxylase alpha chain, mitochondrial precursor
(PCCase alpha subunit) (Propanoyl-CoA:carbon dioxide
ligase alpha subunit)
Length = 703
Score = 108 bits (270), Expect = 4e-24
Identities = 52/81 (64%), Positives = 65/81 (80%)
Frame = +1
Query: 7 KFMLPKKVIDTSSVIIAPMPGLVKSVSVKVGQRVSEGQELCVLEAMKMQNSMVAAKTGVI 186
KFML K DTSSV+ +PMPG+V +VSVK G V+EGQE+CV+EAMKMQNSM A KTG +
Sbjct: 623 KFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTV 682
Query: 187 KSIHFQAGQTVSDDDVIIELE 249
KS+H QAG TV + D+++ELE
Sbjct: 683 KSVHCQAGDTVGEGDLLVELE 703
>sp|Q91ZA3|PCCA_MOUSE Propionyl-CoA carboxylase alpha chain, mitochondrial precursor
(PCCase alpha subunit) (Propanoyl-CoA:carbon dioxide
ligase alpha subunit)
Length = 724
Score = 100 bits (248), Expect = 1e-21
Identities = 48/81 (59%), Positives = 62/81 (76%)
Frame = +1
Query: 7 KFMLPKKVIDTSSVIIAPMPGLVKSVSVKVGQRVSEGQELCVLEAMKMQNSMVAAKTGVI 186
KFML K DTSS + +PMPG+V +VSVK G V+EGQE+CV+EAMKMQNSM A K G +
Sbjct: 644 KFMLEKVPKDTSSTLCSPMPGVVVAVSVKPGDMVAEGQEICVIEAMKMQNSMTAGKMGKV 703
Query: 187 KSIHFQAGQTVSDDDVIIELE 249
K +H +AG TV + D+++ELE
Sbjct: 704 KLVHCKAGDTVGEGDLLVELE 724
>sp|P14882|PCCA_RAT Propionyl-CoA carboxylase alpha chain, mitochondrial precursor
(PCCase alpha subunit) (Propanoyl-CoA:carbon dioxide
ligase alpha subunit)
Length = 704
Score = 98.6 bits (244), Expect = 4e-21
Identities = 48/81 (59%), Positives = 62/81 (76%)
Frame = +1
Query: 7 KFMLPKKVIDTSSVIIAPMPGLVKSVSVKVGQRVSEGQELCVLEAMKMQNSMVAAKTGVI 186
KFML K DTSSV+ +P PG+V +VSVK G V+EGQE+CV+EAMKMQNSM A K G +
Sbjct: 624 KFMLEKVPKDTSSVLRSPKPGVVVAVSVKPGDMVAEGQEICVIEAMKMQNSMTAGKMGKV 683
Query: 187 KSIHFQAGQTVSDDDVIIELE 249
K +H +AG TV + D+++ELE
Sbjct: 684 KLVHCKAGDTVGEGDLLVELE 704
>sp|Q05920|PYC_MOUSE Pyruvate carboxylase, mitochondrial precursor (Pyruvic carboxylase)
(PCB)
Length = 1178
Score = 68.2 bits (165), Expect = 6e-12
Identities = 32/77 (41%), Positives = 46/77 (59%)
Frame = +1
Query: 19 PKKVIDTSSVIIAPMPGLVKSVSVKVGQRVSEGQELCVLEAMKMQNSMVAAKTGVIKSIH 198
PK + D I APMPG V + V G +V++GQ LCVL AMKM+ + + G I+ +H
Sbjct: 1102 PKALKDVKGQIGAPMPGKVIDIKVAAGDKVAKGQPLCVLSAMKMETVVTSPMEGTIRKVH 1161
Query: 199 FQAGQTVSDDDVIIELE 249
T+ DD+I+E+E
Sbjct: 1162 VTKDMTLEGDDLILEIE 1178
>sp|P29337|BCCP_STRMU Biotin carboxyl carrier protein (BCCP)
Length = 130
Score = 68.2 bits (165), Expect = 6e-12
Identities = 33/76 (43%), Positives = 46/76 (60%)
Frame = +1
Query: 19 PKKVIDTSSVIIAPMPGLVKSVSVKVGQRVSEGQELCVLEAMKMQNSMVAAKTGVIKSIH 198
P ++ + APMPG + V V VG VSE Q L +LEAMKM+N +VA G + +IH
Sbjct: 54 PAATASAANTMPAPMPGTILKVLVNVGDTVSENQPLMILEAMKMENEIVAGMAGTVSAIH 113
Query: 199 FQAGQTVSDDDVIIEL 246
+GQTV+ D +I +
Sbjct: 114 VSSGQTVNAGDNLITI 129
>sp|P52873|PYC_RAT Pyruvate carboxylase, mitochondrial precursor (Pyruvic carboxylase)
(PCB)
Length = 1178
Score = 67.0 bits (162), Expect = 1e-11
Identities = 33/77 (42%), Positives = 45/77 (58%)
Frame = +1
Query: 19 PKKVIDTSSVIIAPMPGLVKSVSVKVGQRVSEGQELCVLEAMKMQNSMVAAKTGVIKSIH 198
PK + D I APMPG V V V G +V +GQ LCVL AMKM+ + + G I+ +H
Sbjct: 1102 PKALKDVKGQIGAPMPGKVIDVKVAAGAKVVKGQPLCVLSAMKMETVVTSPMEGTIRKVH 1161
Query: 199 FQAGQTVSDDDVIIELE 249
T+ DD+I+E+E
Sbjct: 1162 VTKDMTLEGDDLILEIE 1178
>sp|P11498|PYC_HUMAN Pyruvate carboxylase, mitochondrial precursor (Pyruvic carboxylase)
(PCB)
Length = 1178
Score = 67.0 bits (162), Expect = 1e-11
Identities = 31/77 (40%), Positives = 46/77 (59%)
Frame = +1
Query: 19 PKKVIDTSSVIIAPMPGLVKSVSVKVGQRVSEGQELCVLEAMKMQNSMVAAKTGVIKSIH 198
PK + D I APMPG V + V G +V++GQ LCVL AMKM+ + + G ++ +H
Sbjct: 1102 PKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVH 1161
Query: 199 FQAGQTVSDDDVIIELE 249
T+ DD+I+E+E
Sbjct: 1162 VTKDMTLEGDDLILEIE 1178
>sp|Q9ZAA7|GCDC_ACIFE Glutaconyl-CoA decarboxylase gamma subunit (Biotin carrier)
Length = 145
Score = 64.7 bits (156), Expect = 6e-11
Identities = 31/69 (44%), Positives = 44/69 (63%)
Frame = +1
Query: 40 SSVIIAPMPGLVKSVSVKVGQRVSEGQELCVLEAMKMQNSMVAAKTGVIKSIHFQAGQTV 219
S + APMPG + SV+VK G +V G L +LEAMKMQN ++A + G + + AG TV
Sbjct: 77 SVTVSAPMPGKILSVNVKPGDKVEAGDVLLILEAMKMQNEIMAPEDGTVSEVRVNAGDTV 136
Query: 220 SDDDVIIEL 246
+ DV++ L
Sbjct: 137 ATGDVMVIL 145
>sp|Q42777|MCCA_SOYBN Methylcrotonoyl-CoA carboxylase alpha chain, mitochondrial
precursor (3-Methylcrotonyl-CoA carboxylase 1) (MCCase
alpha subunit) (3-methylcrotonyl-CoA:carbon dioxide
ligase alpha subunit)
Length = 731
Score = 55.8 bits (133), Expect = 3e-08
Identities = 30/80 (37%), Positives = 47/80 (58%), Gaps = 4/80 (5%)
Frame = +1
Query: 22 KKVIDTSS----VIIAPMPGLVKSVSVKVGQRVSEGQELCVLEAMKMQNSMVAAKTGVIK 189
K ++TS+ ++APM GLV V V+ RV EGQ + VLEAMKM++ + A +G +
Sbjct: 650 KPKVETSANPQGTVVAPMAGLVVKVLVENKTRVEEGQPVLVLEAMKMEHVVKAPSSGYVH 709
Query: 190 SIHFQAGQTVSDDDVIIELE 249
+ G+ VSD V+ ++
Sbjct: 710 GLQLMVGEQVSDGSVLFSVK 729
>sp|Q42523|MCCA_ARATH Methylcrotonoyl-CoA carboxylase alpha chain, mitochondrial
precursor (3-Methylcrotonyl-CoA carboxylase 1) (MCCase
alpha subunit) (3-methylcrotonyl-CoA:carbon dioxide
ligase alpha subunit)
Length = 734
Score = 55.5 bits (132), Expect = 4e-08
Identities = 28/67 (41%), Positives = 43/67 (64%)
Frame = +1
Query: 49 IIAPMPGLVKSVSVKVGQRVSEGQELCVLEAMKMQNSMVAAKTGVIKSIHFQAGQTVSDD 228
I+APM GLV V V+ +V +GQ + VLEAMKM++ + A +G I+ + +AGQ VSD
Sbjct: 667 IVAPMAGLVVKVLVENEAKVDQGQPILVLEAMKMEHVVKAPSSGSIQDLKVKAGQQVSDG 726
Query: 229 DVIIELE 249
+ ++
Sbjct: 727 SALFRIK 733
Score = 28.5 bits (62), Expect = 5.1
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = +1
Query: 46 VIIAPMPGLVKSVSVKVGQRVSEGQEL 126
V+ AP G ++ + VK GQ+VS+G L
Sbjct: 703 VVKAPSSGSIQDLKVKAGQQVSDGSAL 729
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,456,916
Number of Sequences: 369166
Number of extensions: 782293
Number of successful extensions: 1681
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1642
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1680
length of database: 68,354,980
effective HSP length: 96
effective length of database: 50,620,420
effective search space used: 1721094280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)