Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_023_L11
(689 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P55112|NAS4_CAEEL Zinc metalloproteinase nas-4 precursor... 100 4e-21
sp|P55115|NAS15_CAEEL Zinc metalloproteinase nas-15 precurs... 91 4e-18
sp|P31579|LCE_ORYLA Low choriolytic enzyme precursor (Hatch... 89 1e-17
sp|P07584|ASTA_ASTFL Astacin precursor (Crayfish small-mole... 89 1e-17
sp|P31581|HCE21_ORYLA High choriolytic enzyme 2 precursor (... 87 5e-17
sp|P31580|HCE23_ORYLA High choriolytic enzyme 1 precursor (... 87 5e-17
sp|P55113|NAS7_CAEEL Zinc metalloproteinase nas-7 precursor... 85 2e-16
sp|Q20191|NAS13_CAEEL Zinc metalloproteinase nas-13 precurs... 85 2e-16
sp|Q19269|NAS14_CAEEL Zinc metalloproteinase nas-14 precurs... 84 5e-16
sp|Q18439|NAS8_CAEEL Zinc metalloproteinase nas-8 precursor... 84 5e-16
>sp|P55112|NAS4_CAEEL Zinc metalloproteinase nas-4 precursor (Nematode astacin 4)
Length = 315
Score = 100 bits (249), Expect = 4e-21
Identities = 60/193 (31%), Positives = 104/193 (53%), Gaps = 6/193 (3%)
Frame = +2
Query: 98 KRWPNNQVLFSFAPAFPNDKKGIVRECLVELQKDLGNCVKFSESSASHYIEVQSL--DQG 271
+RWPNN++ ++ + + + + ++ + E CVKF S + + + D+G
Sbjct: 102 RRWPNNEIPYTLSSQYGSYARSVIANAMNEYHTK--TCVKFVARDPSKHHDYLWIHPDEG 159
Query: 272 CYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHIYIRWNRIL 451
CYSL+G TGG KQP++L + GC+ GT+ HE +HA+GF H R+DRD++I + W ++
Sbjct: 160 CYSLVGKTGG-KQPVSLDS-GCI-QVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVM 216
Query: 452 SSHCSQFVKCD---GCEVDGPYETHSVMHYPSYGFACVPGEHVVLTSNG-DTIDYNHVTS 619
+ QF K + +D PY+ S+MHY Y F+ + +V +G + + S
Sbjct: 217 NGADDQFEKYNLNVISHLDEPYDYASIMHYGPYAFSGSGKKTLVPKKSGSERMGQRVKFS 276
Query: 620 KNDLDMVRRFYLC 658
D+ + + Y C
Sbjct: 277 DIDVRKINKLYNC 289
>sp|P55115|NAS15_CAEEL Zinc metalloproteinase nas-15 precursor (Nematode astacin 15)
Length = 571
Score = 90.5 bits (223), Expect = 4e-18
Identities = 58/200 (29%), Positives = 100/200 (50%), Gaps = 5/200 (2%)
Frame = +2
Query: 74 FKAHYSNTKRWPNNQVLFSFAPAFPNDKKGIVRECLVELQKDLGNCVKF--SESSASHYI 247
+ A + + WP ++ ++ + + + + ++ + E C+++ E++ +Y+
Sbjct: 113 YNAIKNRLQLWPEGRIPYTISSQYSSYSRSLIAASMQEYASH--TCIRWVPKEAADVNYV 170
Query: 248 EVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHI 427
+ D+GCYS++G GG KQ L+L + GC+ KG + HE +HA+GF H R DRD+HI
Sbjct: 171 HIYP-DRGCYSMVGKMGG-KQSLSLGS-GCI-QKGIILHELMHAVGFFHEQSRTDRDDHI 226
Query: 428 YIRWNRILSSHCSQFVKCDGCEVDG---PYETHSVMHYPSYGFACVPGEHVVLTSNGDTI 598
I WN I + QF K + Y+ S+MHY + F+ ++ NG TI
Sbjct: 227 TIMWNNIQAGMQGQFEKYGHGTIQSLGTGYDYGSIMHYGTKAFSRNGQPTMIPKKNGATI 286
Query: 599 DYNHVTSKNDLDMVRRFYLC 658
+ SK D + Y C
Sbjct: 287 GQRNGFSKVDKFKINTLYGC 306
>sp|P31579|LCE_ORYLA Low choriolytic enzyme precursor (Hatching enzyme zinc-protease LCE
subunit) (Choriolysin L)
Length = 271
Score = 89.0 bits (219), Expect = 1e-17
Identities = 69/228 (30%), Positives = 106/228 (46%), Gaps = 12/228 (5%)
Frame = +2
Query: 11 LNNMQM-----GDMVLDKSDIALINGFKAHYSNTKRWPNN-----QVLFSFAPAFPNDKK 160
+NN M GD+VL K+ A+ F A ++ RWP + +V + + + +D+K
Sbjct: 52 MNNNSMEELLEGDLVLPKTRNAM-KCFGA--PDSCRWPKSSNGIVKVPYVVSDNYESDEK 108
Query: 161 GIVRECLVELQKDLGNCVKF-SESSASHYIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGC 337
+R + E + C+ F ++ Y+ ++ GC S++GY G KQ + LQ GC
Sbjct: 109 ETIRNAMKEFAEK--TCIHFVPRNNERAYLSLEPRF-GCKSMMGYVGD-KQVVVLQRFGC 164
Query: 338 MYSKGTVKHEFIHALGFMHTHMRKDRDNHIYIRWNRILSSHCSQFVKCDGCEVDGPYETH 517
+ ++HE +HALGF H H R DRD H+ I W I+ F K D + PY+
Sbjct: 165 I-KHAVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLGTPYDYG 223
Query: 518 SVMHYPSYGFACVPGEHVVLTSNGD-TIDYNHVTSKNDLDMVRRFYLC 658
S+MHY F E + N I S D+ V + Y C
Sbjct: 224 SIMHYGRTAFGKDRKETITPIPNPKAAIGQTERMSDIDILRVNKLYKC 271
>sp|P07584|ASTA_ASTFL Astacin precursor (Crayfish small-molecule proteinase)
Length = 251
Score = 89.0 bits (219), Expect = 1e-17
Identities = 60/191 (31%), Positives = 98/191 (51%), Gaps = 8/191 (4%)
Frame = +2
Query: 104 WPNNQVLFSFAPAFPNDKKGIVRECLVELQKDLGNCVKF-SESSASHYIEVQSLDQGCYS 280
W + ++FA D+ I+ + EL++ C++F ++ S Y+E+ + GC+S
Sbjct: 59 WSGGVIPYTFAGVSGADQSAILSG-MQELEEK--TCIRFVPRTTESDYVEIFTSGSGCWS 115
Query: 281 LLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHIYIRWNRILSSH 460
+G G +Q ++LQ GC+Y GT+ HE +HA+GF H H R DRDN++ I + + S
Sbjct: 116 YVGRISGAQQ-VSLQANGCVYH-GTIIHELMHAIGFYHEHTRMDRDNYVTINYQNVDPSM 173
Query: 461 CSQF-VKCDGCEVDGPYETHSVMHYPSYGFACVPG--EHVVLTSNG----DTIDYNHVTS 619
S F + V Y+ +S+MHY Y F+ G E +V NG D D H+
Sbjct: 174 TSNFDIDTYSRYVGEDYQYYSIMHYGKYSFSIQWGVLETIVPLQNGIDLTDPYDKAHML- 232
Query: 620 KNDLDMVRRFY 652
+ D + + Y
Sbjct: 233 QTDANQINNLY 243
>sp|P31581|HCE21_ORYLA High choriolytic enzyme 2 precursor (Hatching enzyme zinc-protease
HCE 2 subunit) (Choriolysin H 2)
Length = 279
Score = 86.7 bits (213), Expect = 5e-17
Identities = 48/119 (40%), Positives = 68/119 (57%), Gaps = 1/119 (0%)
Frame = +2
Query: 209 CVKFSESSASH-YIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALG 385
C++F + + +I V S + GCYS LG GG +Q L+L GCMYS G ++HE HALG
Sbjct: 129 CIRFVRRTNEYDFISVVSKN-GCYSELGRKGG-QQELSLNRGGCMYS-GIIQHELNHALG 185
Query: 386 FMHTHMRKDRDNHIYIRWNRILSSHCSQFVKCDGCEVDGPYETHSVMHYPSYGFACVPG 562
F H R DRD+++ I W I+ + F K D ++ PY+ S+MHY F+ G
Sbjct: 186 FQHEQTRSDRDSYVRINWQNIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFSIAYG 244
>sp|P31580|HCE23_ORYLA High choriolytic enzyme 1 precursor (Hatching enzyme zinc-protease
HCE 1 subunit) (Choriolysin H 1)
Length = 270
Score = 86.7 bits (213), Expect = 5e-17
Identities = 52/153 (33%), Positives = 79/153 (51%), Gaps = 3/153 (1%)
Frame = +2
Query: 209 CVKFSESSASH-YIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALG 385
C++F + + +I V S GCYS LG GG +Q L++ GCMYS G ++HE HALG
Sbjct: 120 CIRFVRRTNEYDFISVVS-KTGCYSELGRKGG-QQELSINRGGCMYS-GIIQHELNHALG 176
Query: 386 FMHTHMRKDRDNHIYIRWNRILSSHCSQFVKCDGCEVDGPYETHSVMHYPSYGFACVPGE 565
F H R DRD+++ I W I+ + F K D ++ PY+ S+MHY F+ G
Sbjct: 177 FQHEQTRSDRDSYVRINWENIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFSIAYGR 236
Query: 566 HVV--LTSNGDTIDYNHVTSKNDLDMVRRFYLC 658
+ + + I + S+ D+ + Y C
Sbjct: 237 DSITPIPNPNVPIGQRNGMSRWDITRINVLYNC 269
>sp|P55113|NAS7_CAEEL Zinc metalloproteinase nas-7 precursor (Nematode astacin 7)
Length = 382
Score = 85.1 bits (209), Expect = 2e-16
Identities = 63/211 (29%), Positives = 101/211 (47%), Gaps = 7/211 (3%)
Frame = +2
Query: 47 KSDIALINGFKAHYSN--TKRWPNNQVLFSFAPAFPNDKKGIVRECLVELQKDLGNCVKF 220
KSDI L K + + K WPN ++ ++ +P + ++ ++ + + + + C++F
Sbjct: 68 KSDIRLPRRHKRNGVSRAAKLWPNARIPYAISPHYSPHERALLAKAVKQYHEK--TCIRF 125
Query: 221 --SESSASHYIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALGFMH 394
++ Y+ + +D GC+S +G T G Q L+L N GCM T+ HE +H +GF H
Sbjct: 126 VPRQTGEPDYLFIGKVD-GCFSEVGRTSGV-QVLSLDN-GCM-EYATIIHEMMHVVGFYH 181
Query: 395 THMRKDRDNHIYIRWNRILSSHCSQFVKCDGCEVD---GPYETHSVMHYPSYGFACVPGE 565
H R DRDN I I W I QF K D + PY+ S++HY S F+
Sbjct: 182 EHERWDRDNFIDIIWQNIDRGALDQFGKVDLSKTSYYGQPYDYKSILHYDSLAFSKNGFP 241
Query: 566 HVVLTSNGDTIDYNHVTSKNDLDMVRRFYLC 658
++ TI S D+ + R Y C
Sbjct: 242 TMLPKVKSATIGNARDFSDVDISKINRMYNC 272
>sp|Q20191|NAS13_CAEEL Zinc metalloproteinase nas-13 precursor (Nematode astacin 13)
Length = 527
Score = 84.7 bits (208), Expect = 2e-16
Identities = 58/191 (30%), Positives = 92/191 (48%), Gaps = 5/191 (2%)
Frame = +2
Query: 101 RWPNNQVLFSFAPAFPNDKKGIVRECLVELQKDLGNCVKFSESSASH--YIEVQSLDQGC 274
+W ++ ++ + + + + + E + E +K C+ FS SA YI + D GC
Sbjct: 195 KWEQARIPYTISSQYSSYSRSKIAEAIEEYRKK--TCIDFSPKSAGDLDYIHIVP-DDGC 251
Query: 275 YSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHIYIRWNRILS 454
YSL+G GG KQP++L + GC+ KG + HE +HA+GF H R DRD ++ I W+ + +
Sbjct: 252 YSLVGRIGG-KQPVSLGD-GCI-QKGIIIHELMHAVGFFHEQSRADRDEYVKINWSNVEA 308
Query: 455 SHCSQFVKCDGCEVD---GPYETHSVMHYPSYGFACVPGEHVVLTSNGDTIDYNHVTSKN 625
QF K +D Y+ SVMHY F+ + I S+N
Sbjct: 309 GLQDQFDKYSLNMIDHLGTKYDYGSVMHYAPTAFSKNGKPTIEPIEKNVEIGQRAGFSEN 368
Query: 626 DLDMVRRFYLC 658
D+ + Y C
Sbjct: 369 DIYKINMLYNC 379
>sp|Q19269|NAS14_CAEEL Zinc metalloproteinase nas-14 precursor (Nematode astacin 14)
Length = 503
Score = 83.6 bits (205), Expect = 5e-16
Identities = 59/195 (30%), Positives = 89/195 (45%), Gaps = 6/195 (3%)
Frame = +2
Query: 98 KRWPNNQVLFSFAPAFPNDKKGIVRECLVELQKDLGNCVKFSESSASH--YIEVQ-SLDQ 268
K WP QV + ND++ + + E + CV+F + YI V+ ++
Sbjct: 123 KLWPEGQVPYMLEEGMTNDQRTAIAQAFDEYKTK--TCVRFVPKTDDDFDYIYVKRNVAF 180
Query: 269 GCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHIYIRWNRI 448
GC S +G GG Q ++L+ C +SKG + HE +HALGF H H R DRD+ + I + I
Sbjct: 181 GCSSYVGRAGG-NQTVSLEVDKC-FSKGIIAHELMHALGFFHEHSRTDRDDFVDINEDNI 238
Query: 449 LSSHCSQFVKCDGCEVDG---PYETHSVMHYPSYGFACVPGEHVVLTSNGDTIDYNHVTS 619
F K +D PY+ SVMHY F+ ++ N + + S
Sbjct: 239 RPGMMRNFEKYPRKIIDSLGMPYDYESVMHYHKLAFSRNGKPTIIPKDNEADVGQRYKLS 298
Query: 620 KNDLDMVRRFYLC*E 664
+ D V + Y C E
Sbjct: 299 EMDSKKVNKLYQCGE 313
>sp|Q18439|NAS8_CAEEL Zinc metalloproteinase nas-8 precursor (Nematode astacin 8)
Length = 403
Score = 83.6 bits (205), Expect = 5e-16
Identities = 56/159 (35%), Positives = 85/159 (53%), Gaps = 5/159 (3%)
Frame = +2
Query: 89 SNTKRWPNNQVLFSFAPAFPNDKKGIVRECLVELQKDLGNCVKFSESSA--SHYIEVQSL 262
+ T++WPN ++ + + + ND++ V + D CV+F +A + Y+ + +
Sbjct: 116 TGTRKWPNGRIPYVISNQY-NDRERAVLARSFQAYHDK-TCVRFVPRTAVDNDYLYIGKI 173
Query: 263 DQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHIYIRWN 442
D GCYS +G GG +Q L+L N GC+ T HE +H++GF H H R DRD HI I W+
Sbjct: 174 D-GCYSDVGRAGG-RQELSLDN-GCL-QYDTAIHELMHSVGFYHEHERWDRDEHITILWH 229
Query: 443 RILSSHCSQFVKCDGCE---VDGPYETHSVMHYPSYGFA 550
I QF K D E Y+ +S+MHY S F+
Sbjct: 230 NIDREAYDQFGKVDLAESSYYGQLYDYYSIMHYDSLAFS 268
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85,449,617
Number of Sequences: 369166
Number of extensions: 1816070
Number of successful extensions: 4613
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4402
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4555
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5927776870
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)