Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_023_H24
(403 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q12931|TRAP1_HUMAN Heat shock protein 75 kDa, mitochondr... 100 1e-21
sp|Q9CQN1|TRAP1_MOUSE Heat shock protein 75 kDa, mitochondr... 99 2e-21
sp|P06660|HSP85_TRYCR Heat shock-like 85 kDa protein 45 7e-05
sp|P12861|HSP83_TRYBB Heat shock protein 83 41 8e-04
sp|P27741|HSP83_LEIAM Heat shock protein 83 (HSP 83) 41 8e-04
sp|P27890|HSP83_LEIDO Heat shock protein 83 (HSP 83) (HSP 90) 40 0.001
sp|O57521|HS90B_BRARE Heat shock protein HSP 90-beta 38 0.009
sp|Q25293|HSP83_LEIIN Heat shock protein 83-1 (HSP 83) 37 0.011
sp|O18750|ENPL_RABIT Endoplasmin (94 kDa glucose-regulated ... 37 0.015
sp|P27323|HSP81_ARATH Heat shock protein 81-1 (HSP81-1) (He... 35 0.055
>sp|Q12931|TRAP1_HUMAN Heat shock protein 75 kDa, mitochondrial precursor (HSP 75) (Tumor
necrosis factor type 1 receptor associated protein)
(TRAP-1) (TNFR-associated protein 1)
Length = 704
Score = 100 bits (248), Expect = 1e-21
Identities = 51/116 (43%), Positives = 71/116 (61%)
Frame = +2
Query: 11 RKQMKPKVATVKVTQRLTTHPCLVSVKNMGAVRHFIKTSLIDKSDEEKYMLMNATMEINP 190
R + +V VKVT RL THP +V+V MGA RHF++ + K+ EE+ L+ T+EINP
Sbjct: 587 RNVLGSRVTNVKVTLRLDTHPAMVTVLEMGAARHFLRMQQLAKTQEERAQLLQPTLEINP 646
Query: 191 TXXXXXXXXXXXXTDEDLAALLINQLYQNALIQAGLVTDSRPIIGDLNVLLTKAFE 358
++ LA LL++Q+Y+NA+I AGLV D R ++G LN LL KA E
Sbjct: 647 RHALIKKLNQLRASEPGLAQLLVDQIYENAMIAAGLVDDPRAMVGRLNELLVKALE 702
>sp|Q9CQN1|TRAP1_MOUSE Heat shock protein 75 kDa, mitochondrial precursor (HSP 75) (Tumor
necrosis factor type 1 receptor associated protein)
(TRAP-1) (TNFR-associated protein 1)
Length = 706
Score = 99.4 bits (246), Expect = 2e-21
Identities = 50/116 (43%), Positives = 71/116 (61%)
Frame = +2
Query: 11 RKQMKPKVATVKVTQRLTTHPCLVSVKNMGAVRHFIKTSLIDKSDEEKYMLMNATMEINP 190
R + +V VKVT RL THP +V+V MGA RHF++ + K+ EE+ L+ T+EINP
Sbjct: 589 RNALGSRVTNVKVTFRLDTHPAMVTVLEMGAARHFLRMQQLAKTQEERAQLLQPTLEINP 648
Query: 191 TXXXXXXXXXXXXTDEDLAALLINQLYQNALIQAGLVTDSRPIIGDLNVLLTKAFE 358
++ +LA LL++Q+Y+NA+I AGLV D R ++G LN LL K E
Sbjct: 649 RHTLIKKLCQLRESEPELAQLLVDQIYENAMIAAGLVDDPRAMVGRLNDLLVKVLE 704
>sp|P06660|HSP85_TRYCR Heat shock-like 85 kDa protein
Length = 704
Score = 44.7 bits (104), Expect = 7e-05
Identities = 29/98 (29%), Positives = 43/98 (43%), Gaps = 5/98 (5%)
Frame = +2
Query: 29 KVATVKVTQRLTTHPCLVSVKNMGAVRHF---IKTSLIDKSDEEKYMLMNATMEINPTXX 199
KV V V++RL T PC++ G H ++ + S YM+ TMEINP
Sbjct: 557 KVEKVVVSERLATSPCILVTSEFGWSAHMEQIMRNQALRDSSMSAYMMSKKTMEINPAHP 616
Query: 200 XXXXXXXXXXTDEDLAAL--LINQLYQNALIQAGLVTD 307
DE+ A+ L+ L+ AL+ +G D
Sbjct: 617 IVKELKRRVEADENDKAVKDLVYLLFDTALLTSGFTLD 654
>sp|P12861|HSP83_TRYBB Heat shock protein 83
Length = 703
Score = 41.2 bits (95), Expect = 8e-04
Identities = 30/105 (28%), Positives = 42/105 (40%), Gaps = 5/105 (4%)
Frame = +2
Query: 29 KVATVKVTQRLTTHPCLVSVKNMGAVRHF---IKTSLIDKSDEEKYMLMNATMEINPTXX 199
KV V V+ RL T PC++ G H ++ + S YM+ TMEIN T
Sbjct: 557 KVEKVVVSDRLATSPCILVTSEFGWSAHMEQIMRNQALRDSSMSAYMMRKKTMEINTTHA 616
Query: 200 XXXXXXXXXXTDED--LAALLINQLYQNALIQAGLVTDSRPIIGD 328
DE+ A LI L+ +L+ +G D D
Sbjct: 617 IVKELKRRVEADENDKAAKDLIFLLFDTSLLTSGFTLDDPTAYAD 661
>sp|P27741|HSP83_LEIAM Heat shock protein 83 (HSP 83)
Length = 701
Score = 41.2 bits (95), Expect = 8e-04
Identities = 26/94 (27%), Positives = 44/94 (46%), Gaps = 5/94 (5%)
Frame = +2
Query: 29 KVATVKVTQRLTTHPCLVSVKNMGAVRHF---IKTSLIDKSDEEKYMLMNATMEINPTXX 199
KV V V++RL+T PC++ G H ++ + S +YM+ TME+NP
Sbjct: 554 KVEKVTVSERLSTSPCILVTSEFGWSAHMEQMMRNQALRDSSMAQYMMSKKTMELNPKHP 613
Query: 200 XXXXXXXXXXTDEDLAAL--LINQLYQNALIQAG 295
DE+ A+ L+ L+ +L+ +G
Sbjct: 614 IIKELRRRVEADENDKAVKDLVFLLFDTSLLTSG 647
>sp|P27890|HSP83_LEIDO Heat shock protein 83 (HSP 83) (HSP 90)
Length = 452
Score = 40.4 bits (93), Expect = 0.001
Identities = 26/94 (27%), Positives = 42/94 (44%), Gaps = 5/94 (5%)
Frame = +2
Query: 29 KVATVKVTQRLTTHPCLVSVKNMGAVRHF---IKTSLIDKSDEEKYMLMNATMEINPTXX 199
KV V V+ RL+T PC++ G H ++ + S YM+ TME+NP
Sbjct: 306 KVEKVTVSDRLSTSPCILVTSEFGWSAHMEQIMRNQAVRDSSMSAYMMSKKTMELNPRHP 365
Query: 200 XXXXXXXXXXTDEDLAAL--LINQLYQNALIQAG 295
DE+ A+ L+ L+ +L+ +G
Sbjct: 366 IIKVLRRRVEADENDKAVKDLVFLLFDTSLLTSG 399
>sp|O57521|HS90B_BRARE Heat shock protein HSP 90-beta
Length = 725
Score = 37.7 bits (86), Expect = 0.009
Identities = 25/98 (25%), Positives = 42/98 (42%), Gaps = 5/98 (5%)
Frame = +2
Query: 29 KVATVKVTQRLTTHPCLVSVKNMG---AVRHFIKTSLIDKSDEEKYMLMNATMEINPTXX 199
KV V V+ RL + PC + G + +K + + YM+ N +EINP
Sbjct: 573 KVEKVTVSNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMANKHLEINPDHP 632
Query: 200 XXXXXXXXXXTDEDLAAL--LINQLYQNALIQAGLVTD 307
D++ A+ L+ L++ AL+ +G D
Sbjct: 633 IMETLRQKAEADKNTKAVKDLVILLFETALLSSGFSLD 670
>sp|Q25293|HSP83_LEIIN Heat shock protein 83-1 (HSP 83)
Length = 701
Score = 37.4 bits (85), Expect = 0.011
Identities = 25/94 (26%), Positives = 43/94 (45%), Gaps = 5/94 (5%)
Frame = +2
Query: 29 KVATVKVTQRLTTHPCLVSVKNMGAVRHF---IKTSLIDKSDEEKYMLMNATMEINPTXX 199
KV V V++ L+T PC++ G H ++ + S +YM+ TME+NP
Sbjct: 553 KVEKVIVSECLSTSPCILVTSEFGWSAHMEQIMRNQALRDSSMAQYMMSKKTMELNPRHP 612
Query: 200 XXXXXXXXXXTDEDLAAL--LINQLYQNALIQAG 295
DE+ A+ L+ L+ +L+ +G
Sbjct: 613 IIKELRRRVDADENDKAVKDLVFLLFDTSLLTSG 646
>sp|O18750|ENPL_RABIT Endoplasmin (94 kDa glucose-regulated protein) (GRP94)
Length = 716
Score = 37.0 bits (84), Expect = 0.015
Identities = 27/109 (24%), Positives = 43/109 (39%), Gaps = 4/109 (3%)
Frame = +2
Query: 14 KQMKPKVATVKVTQRLTTHPCLVSVKNMG---AVRHFIKTSLIDK-SDEEKYMLMNATME 181
K +K K+ V+QRLT PC + G + +K D KY T E
Sbjct: 544 KALKDKIEKAVVSQRLTESPCALVASQYGWSANMERIMKAQAYQTGKDSTKYYASQKTFE 603
Query: 182 INPTXXXXXXXXXXXXTDEDLAALLINQLYQNALIQAGLVTDSRPIIGD 328
INP D+ + L + L++ A++++G + GD
Sbjct: 604 INPRHPLIRDMLRIKEDDKTVMDLAV-VLFETAILRSGYLLPDTKAYGD 651
>sp|P27323|HSP81_ARATH Heat shock protein 81-1 (HSP81-1) (Heat shock protein 83)
Length = 700
Score = 35.0 bits (79), Expect = 0.055
Identities = 26/98 (26%), Positives = 40/98 (40%), Gaps = 5/98 (5%)
Frame = +2
Query: 29 KVATVKVTQRLTTHPCLVSVKNMG---AVRHFIKTSLIDKSDEEKYMLMNATMEINPTXX 199
KV V V+ R+ PC + G + +K + S YM TMEINP
Sbjct: 555 KVEKVVVSDRIVDSPCCLVTGEYGWTANMERIMKAQALRDSSMSGYMSSKKTMEINPDNG 614
Query: 200 XXXXXXXXXXTDEDLAAL--LINQLYQNALIQAGLVTD 307
D++ ++ L+ LY+ AL+ +G D
Sbjct: 615 IMEELRKRAEADKNDKSVKDLVMLLYETALLTSGFSLD 652
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 45,908,071
Number of Sequences: 369166
Number of extensions: 881726
Number of successful extensions: 2214
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2214
length of database: 68,354,980
effective HSP length: 98
effective length of database: 50,250,950
effective search space used: 1758783250
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)