Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_023_F24
(205 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P16333|NCK1_HUMAN Cytoplasmic protein NCK1 (NCK adaptor ... 65 5e-11
sp|O43639|NCK2_HUMAN Cytoplasmic protein NCK2 (NCK adaptor ... 62 3e-10
sp|P08630|BTKL_DROME Tyrosine-protein kinase Btk29A (Dsrc28C) 54 1e-07
sp|Q08509|EPS8_MOUSE Epidermal growth factor receptor kinas... 53 2e-07
sp|Q12929|EPS8_HUMAN Epidermal growth factor receptor kinas... 52 4e-07
sp|Q5R4H4|EPS8_PONPY Epidermal growth factor receptor kinas... 52 4e-07
sp|Q08881|ITK_HUMAN Tyrosine-protein kinase ITK/TSK (T-cell... 50 1e-06
sp|P24604|TEC_MOUSE Tyrosine-protein kinase Tec 50 2e-06
sp|P42680|TEC_HUMAN Tyrosine-protein kinase Tec 49 4e-06
sp|P06241|FYN_HUMAN Proto-oncogene tyrosine-protein kinase ... 49 5e-06
>sp|P16333|NCK1_HUMAN Cytoplasmic protein NCK1 (NCK adaptor protein 1) (SH2/SH3 adaptor
protein NCK-alpha)
Length = 377
Score = 65.1 bits (157), Expect = 5e-11
Identities = 29/50 (58%), Positives = 37/50 (74%)
Frame = +2
Query: 53 MAVAKFDYKAVDLHELNINKGEKLTLVDDSQHWWQVMNSKGETGYVPSNY 202
+ VAKFDY A EL+I K E+L L+DDS+ WW+V NS +TG+VPSNY
Sbjct: 6 VVVAKFDYVAQQEQELDIKKNERLWLLDDSKSWWRVRNSMNKTGFVPSNY 55
Score = 33.9 bits (76), Expect = 0.12
Identities = 15/57 (26%), Positives = 29/57 (50%), Gaps = 3/57 (5%)
Frame = +2
Query: 41 QPVGMAVAKFDYKAVDLHELNINKGEKLTLVDDSQH---WWQVMNSKGETGYVPSNY 202
Q + + A + + + + ELN KG+ + +++ ++ WW+ G G VP NY
Sbjct: 190 QVLHVVQALYPFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNY 246
>sp|O43639|NCK2_HUMAN Cytoplasmic protein NCK2 (NCK adaptor protein 2) (SH2/SH3 adaptor
protein NCK-beta) (Nck-2)
Length = 380
Score = 62.4 bits (150), Expect = 3e-10
Identities = 27/50 (54%), Positives = 36/50 (72%)
Frame = +2
Query: 53 MAVAKFDYKAVDLHELNINKGEKLTLVDDSQHWWQVMNSKGETGYVPSNY 202
+ +AK+DY A EL+I K E+L L+DDS+ WW+V N+ TGYVPSNY
Sbjct: 6 IVIAKWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNAANRTGYVPSNY 55
Score = 42.0 bits (97), Expect = 4e-04
Identities = 16/48 (33%), Positives = 29/48 (60%), Gaps = 3/48 (6%)
Frame = +2
Query: 68 FDYKAVDLHELNINKGEKLTLVDDSQH---WWQVMNSKGETGYVPSNY 202
+ + +V ELN KGE + +++ ++ WW+ N++G+ G VP NY
Sbjct: 204 YPFSSVTEEELNFEKGETMEVIEKPENDPEWWKCKNARGQVGLVPKNY 251
>sp|P08630|BTKL_DROME Tyrosine-protein kinase Btk29A (Dsrc28C)
Length = 786
Score = 53.9 bits (128), Expect = 1e-07
Identities = 22/53 (41%), Positives = 37/53 (69%), Gaps = 1/53 (1%)
Frame = +2
Query: 47 VGMAVAKFDYKAVDLHELNINKGEKLTLVDDSQ-HWWQVMNSKGETGYVPSNY 202
V + VA + +KA++ +L++ K + ++DDSQ HWW+V ++ G GY+PSNY
Sbjct: 344 VKLVVALYPFKAIEGGDLSLEKNAEYEVIDDSQEHWWKVKDALGNVGYIPSNY 396
>sp|Q08509|EPS8_MOUSE Epidermal growth factor receptor kinase substrate 8
Length = 821
Score = 53.1 bits (126), Expect = 2e-07
Identities = 21/53 (39%), Positives = 37/53 (69%)
Frame = +2
Query: 41 QPVGMAVAKFDYKAVDLHELNINKGEKLTLVDDSQHWWQVMNSKGETGYVPSN 199
QP A +K+D+ A + EL++ K + L ++DD + WW+V N+ G++G+VP+N
Sbjct: 530 QPKKYAKSKYDFVARNSSELSVMKDDVLEILDDRRQWWKVRNASGDSGFVPNN 582
>sp|Q12929|EPS8_HUMAN Epidermal growth factor receptor kinase substrate 8
Length = 822
Score = 52.0 bits (123), Expect = 4e-07
Identities = 21/53 (39%), Positives = 37/53 (69%)
Frame = +2
Query: 41 QPVGMAVAKFDYKAVDLHELNINKGEKLTLVDDSQHWWQVMNSKGETGYVPSN 199
QP A +K+D+ A + EL++ K + L ++DD + WW+V N+ G++G+VP+N
Sbjct: 531 QPKKYAKSKYDFVARNNSELSVLKDDILEILDDRKQWWKVRNASGDSGFVPNN 583
>sp|Q5R4H4|EPS8_PONPY Epidermal growth factor receptor kinase substrate 8
Length = 822
Score = 52.0 bits (123), Expect = 4e-07
Identities = 21/53 (39%), Positives = 37/53 (69%)
Frame = +2
Query: 41 QPVGMAVAKFDYKAVDLHELNINKGEKLTLVDDSQHWWQVMNSKGETGYVPSN 199
QP A +K+D+ A + EL++ K + L ++DD + WW+V N+ G++G+VP+N
Sbjct: 531 QPKKYAKSKYDFVARNNSELSVLKDDILEILDDRKQWWKVRNASGDSGFVPNN 583
>sp|Q08881|ITK_HUMAN Tyrosine-protein kinase ITK/TSK (T-cell-specific kinase)
(Tyrosine-protein kinase Lyk) (Kinase EMT)
Length = 620
Score = 50.4 bits (119), Expect = 1e-06
Identities = 21/51 (41%), Positives = 33/51 (64%), Gaps = 1/51 (1%)
Frame = +2
Query: 53 MAVAKFDYKAVDLHELNINKGEKLTLVDDSQ-HWWQVMNSKGETGYVPSNY 202
+ +A +DY+ D EL + + E+ L+D S+ HWW+V + G GYVPS+Y
Sbjct: 175 VVIALYDYQTNDPQELALRRNEEYCLLDSSEIHWWRVQDRNGHEGYVPSSY 225
>sp|P24604|TEC_MOUSE Tyrosine-protein kinase Tec
Length = 630
Score = 50.1 bits (118), Expect = 2e-06
Identities = 17/51 (33%), Positives = 34/51 (66%), Gaps = 1/51 (1%)
Frame = +2
Query: 53 MAVAKFDYKAVDLHELNINKGEKLTLVDDSQ-HWWQVMNSKGETGYVPSNY 202
+ VA +D++A + H+L + +G++ +++ + HWW+ + G GY+PSNY
Sbjct: 182 IVVAMYDFQATEAHDLRLERGQEYIILEKNDLHWWRARDKYGSEGYIPSNY 232
>sp|P42680|TEC_HUMAN Tyrosine-protein kinase Tec
Length = 631
Score = 48.9 bits (115), Expect = 4e-06
Identities = 17/51 (33%), Positives = 34/51 (66%), Gaps = 1/51 (1%)
Frame = +2
Query: 53 MAVAKFDYKAVDLHELNINKGEKLTLVDDSQ-HWWQVMNSKGETGYVPSNY 202
+ VA +D++A + H+L + +G++ +++ + HWW+ + G GY+PSNY
Sbjct: 183 IVVAMYDFQAAEGHDLRLERGQEYLILEKNDVHWWRARDKYGNEGYIPSNY 233
>sp|P06241|FYN_HUMAN Proto-oncogene tyrosine-protein kinase Fyn (p59-Fyn) (Protooncogene
Syn) (SLK)
Length = 537
Score = 48.5 bits (114), Expect = 5e-06
Identities = 23/54 (42%), Positives = 37/54 (68%), Gaps = 2/54 (3%)
Frame = +2
Query: 47 VGMAVAKFDYKAVDLHELNINKGEKLTLVDDSQ-HWWQVMN-SKGETGYVPSNY 202
V + VA +DY+A +L+ +KGEK +++ S+ WW+ + + GETGY+PSNY
Sbjct: 84 VTLFVALYDYEARTEDDLSFHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNY 137
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,562,997
Number of Sequences: 369166
Number of extensions: 376954
Number of successful extensions: 1290
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1191
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1230
length of database: 68,354,980
effective HSP length: 39
effective length of database: 61,150,315
effective search space used: 1712208820
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)