Planarian EST Database


Dr_sW_023_F20

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_023_F20
         (859 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9D1E6|TBCB_MOUSE  Tubulin-specific chaperone B (Tubulin ...   206   7e-53
sp|Q5E951|TBCB_BOVIN  Tubulin-specific chaperone B (Tubulin ...   204   3e-52
sp|Q99426|TBCB_HUMAN  Tubulin-specific chaperone B (Tubulin ...   201   2e-51
sp|Q20728|YXHK_CAEEL  Hypothetical protein F53F4.3 in chromo...   176   6e-44
sp|Q10235|ALP11_SCHPO  Cell polarity protein alp11 (Altered ...    94   6e-19
sp|Q01397|DYNA_NEUCR  Dynactin, 150 kDa isoform (150 kDa dyn...    70   1e-11
sp|Q9VJE5|CL190_DROME  Restin homolog (Cytoplasmic linker pr...    69   1e-11
sp|Q8CI96|RSNL2_MOUSE  Restin-like protein 2                       67   5e-11
sp|Q8N3C7|RSNL2_HUMAN  Restin-like protein 2                       67   5e-11
sp|Q66HD5|RSNL2_RAT  Restin-like protein 2                         67   5e-11
>sp|Q9D1E6|TBCB_MOUSE Tubulin-specific chaperone B (Tubulin folding cofactor B)
           (Cytoskeleton associated protein 1)
           (Cytoskeleton-associated protein CKAPI)
          Length = 244

 Score =  206 bits (524), Expect = 7e-53
 Identities = 105/238 (44%), Positives = 151/238 (63%), Gaps = 3/238 (1%)
 Frame = +3

Query: 3   ISLFITNNATSVQCEKRYPCNLTVGEFKAKLELVTGCDHRHVKLQVFTEDDEPFCWLDDN 182
           + +FI+++  S + EKRY  +LT+ EFK KLELV G     ++L+++  DD+ +  LD  
Sbjct: 11  VMVFISSSLNSFRSEKRYSRSLTIAEFKCKLELVVGSPASCMELELYGADDKFYSKLDQE 70

Query: 183 MKALGDYPIKDKMRVHATDPN-LKGGEFETLDESQPLYEISFEEYAKREDTVLAFKRRMK 359
              LG YP+ D  R+H  D + ++ GE+E + + +  YEIS E Y +R++TV +F +R K
Sbjct: 71  DALLGSYPVDDGCRIHVIDHSGVRLGEYEDVSKVEK-YEISPEAYERRQNTVRSFMKRSK 129

Query: 360 LGQFREVDPXXXXXXXXXXXXXLQDEKDKAESLKISDRCEVRVPNQSAKRGQIKYIGTTH 539
           LG + E                L +EK +A ++ +  RCEVR P+ S +RG + Y+G T 
Sbjct: 130 LGPYNE---ELRAQQEAEAAQRLSEEKAQASAISVGSRCEVRAPDHSLRRGTVMYVGLTD 186

Query: 540 FKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHITAGDFPE--LGLDDL 707
           FKPGYWVGV YDEP GKN+G+V+GK YFEC  KYGAF+KP  +T GDFPE   GLD++
Sbjct: 187 FKPGYWVGVRYDEPLGKNDGSVNGKRYFECQAKYGAFVKPSAVTVGDFPEEDYGLDEM 244
>sp|Q5E951|TBCB_BOVIN Tubulin-specific chaperone B (Tubulin folding cofactor B)
           (Cytoskeleton associated protein 1)
           (Cytoskeleton-associated protein CKAPI)
          Length = 244

 Score =  204 bits (519), Expect = 3e-52
 Identities = 102/238 (42%), Positives = 153/238 (64%), Gaps = 3/238 (1%)
 Frame = +3

Query: 3   ISLFITNNATSVQCEKRYPCNLTVGEFKAKLELVTGCDHRHVKLQVFTEDDEPFCWLDDN 182
           +++FI+++  S + +KRY  +LTV EFK KL+LV G     ++L+++  DD+  C LD +
Sbjct: 11  VNVFISSSLNSFRSQKRYSRSLTVAEFKCKLQLVVGSPASCMELELYGPDDKFCCKLDQD 70

Query: 183 MKALGDYPIKDKMRVHATDPN-LKGGEFETLDESQPLYEISFEEYAKREDTVLAFKRRMK 359
              LG YP+ D  R+H  D +  + GE+E + + +  YEIS E Y +R+D++ +F +R K
Sbjct: 71  DALLGSYPVDDGCRIHVIDHSGARLGEYEDISKVEK-YEISQEAYEQRQDSIRSFLKRNK 129

Query: 360 LGQFREVDPXXXXXXXXXXXXXLQDEKDKAESLKISDRCEVRVPNQSAKRGQIKYIGTTH 539
           LG+F E +              L +E+ +A ++ +  RCEVR P Q  +RG + Y+G T 
Sbjct: 130 LGRFNEEE---RAQQEAENSQRLIEEEAQASTIPVGSRCEVRTPGQPPRRGTVMYVGLTD 186

Query: 540 FKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHITAGDFPE--LGLDDL 707
           FKPGYW+G+ YDEP GKN+G+V+GK YFEC  KYGAF+KP  +T GDFPE   GLD++
Sbjct: 187 FKPGYWIGIRYDEPLGKNDGSVNGKRYFECQAKYGAFVKPSVVTVGDFPEEDYGLDEM 244
>sp|Q99426|TBCB_HUMAN Tubulin-specific chaperone B (Tubulin folding cofactor B)
           (Cytoskeleton associated protein 1)
           (Cytoskeleton-associated protein CKAPI)
          Length = 244

 Score =  201 bits (511), Expect = 2e-51
 Identities = 103/238 (43%), Positives = 150/238 (63%), Gaps = 3/238 (1%)
 Frame = +3

Query: 3   ISLFITNNATSVQCEKRYPCNLTVGEFKAKLELVTGCDHRHVKLQVFTEDDEPFCWLDDN 182
           +++FI+++  + + EKRY  +LT+ EFK KLEL+ G     ++L+++  DD+ +  LD  
Sbjct: 11  VTVFISSSLNTFRSEKRYSRSLTIAEFKCKLELLVGSPASCMELELYGVDDKFYSKLDQE 70

Query: 183 MKALGDYPIKDKMRVHATDPN-LKGGEFETLDESQPLYEISFEEYAKREDTVLAFKRRMK 359
              LG YP+ D  R+H  D +  + GE+E +   +  Y IS E Y +R+DTV +F +R K
Sbjct: 71  DALLGSYPVDDGCRIHVIDHSGARLGEYEDVSRVEK-YTISQEAYDQRQDTVRSFLKRSK 129

Query: 360 LGQFREVDPXXXXXXXXXXXXXLQDEKDKAESLKISDRCEVRVPNQSAKRGQIKYIGTTH 539
           LG++ E +              L +EK +A S+ +  RCEVR   QS +RG + Y+G T 
Sbjct: 130 LGRYNEEE---RAQQEAEAAQRLAEEKAQASSIPVGSRCEVRAAGQSPRRGTVMYVGLTD 186

Query: 540 FKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHITAGDFPE--LGLDDL 707
           FKPGYW+GV YDEP GKN+G+V+GK YFEC  KYGAF+KP  +T GDFPE   GLD++
Sbjct: 187 FKPGYWIGVRYDEPLGKNDGSVNGKRYFECQAKYGAFVKPAVVTVGDFPEEDYGLDEI 244
>sp|Q20728|YXHK_CAEEL Hypothetical protein F53F4.3 in chromosome V
          Length = 229

 Score =  176 bits (447), Expect = 6e-44
 Identities = 96/239 (40%), Positives = 140/239 (58%), Gaps = 6/239 (2%)
 Frame = +3

Query: 9   LFITNNATSVQCEKRYPCNLTVGEFKAKLELVTGCDHRHVKLQVFTEDDEPFCWLDDNMK 188
           L IT NAT    EK+YP  +++ + K KLELV G     +++Q+F  DD+    L D  K
Sbjct: 7   LEITTNATDFPMEKKYPAGMSLNDLKKKLELVVGTTVDSMRIQLFDGDDQLKGELTDGAK 66

Query: 189 ALGDYPIKDKMRVHATDPNLKGGEFETLDESQ-PLYEISFEEYAKREDTVLAFKRRMKLG 365
           +L D  ++D  R+HA D  + GG  +  DES    YE+S + Y KR D+V A+K++M+  
Sbjct: 67  SLKDLGVRDGYRIHAVD--VTGGNEDFKDESMVEKYEMSDDTYGKRTDSVRAWKKKMQEE 124

Query: 366 QFREVDPXXXXXXXXXXXXXLQDEKDK-----AESLKISDRCEVRVPNQSAKRGQIKYIG 530
           Q                   +++E DK     A+++ + +RCEV V  Q A+RG++ Y+G
Sbjct: 125 Q--------------GSAAPMENESDKLNEEAAKNIMVGNRCEVTVGAQMARRGEVAYVG 170

Query: 531 TTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHITAGDFPELGLDDL 707
            T FK G WVGV YDEP GKN+G+V G  YF+C  KYG F++P  +  GDFPEL +D++
Sbjct: 171 ATKFKEGVWVGVKYDEPVGKNDGSVAGVRYFDCDPKYGGFVRPVDVKVGDFPELSIDEI 229
>sp|Q10235|ALP11_SCHPO Cell polarity protein alp11 (Altered polarity protein 11)
          Length = 234

 Score = 93.6 bits (231), Expect = 6e-19
 Identities = 70/241 (29%), Positives = 107/241 (44%), Gaps = 13/241 (5%)
 Frame = +3

Query: 3   ISLFITNNATSVQCEKRYPCNLTVGEFKAKLELVTGCDHRHVKL-----------QVFTE 149
           I+LFI   ++S   E+R     TV + K KL  + G   ++ KL            VFT 
Sbjct: 4   ITLFI--KSSSANAERRINPQWTVSQLKTKLVPIVGTPEQYQKLTYEPASSTVPGHVFTS 61

Query: 150 DDEPFCWLDDNMKALGDYPIKDKMRVHATDPNLKGGEFETLDESQPLYEISFEEYAKRED 329
           ++E     +  ++ LG   ++D    H     L   +   +D+    Y +  E+Y  R D
Sbjct: 62  EEENLDLGEFKLQPLGTIVVEDTRPPHL---RLDFDDLSQVDK----YVMpreQYENRTD 114

Query: 330 TVLAFKRRMKLGQFREVDPXXXXXXXXXXXXXLQDEKDKAESLKISDRCEVRVPNQSAKR 509
           +V A+K+R +LG+F                   + E  K E + +      R      + 
Sbjct: 115 SVYAWKKRNQLGRFNP---------DFEASKASRQESLKRELVDLQKNLNSRCCAAGERY 165

Query: 510 GQIKYIGTTHF--KPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHITAGDF 683
           G I+YIG          WVGV +DEP GKN+GTV GK YF    K+G+F++   +  GDF
Sbjct: 166 GTIRYIGLVPEINNDNLWVGVEFDEPVGKNDGTVSGKRYFNAKNKHGSFLRSSEVEVGDF 225

Query: 684 P 686
           P
Sbjct: 226 P 226
>sp|Q01397|DYNA_NEUCR Dynactin, 150 kDa isoform (150 kDa dynein-associated polypeptide)
           (DP-150) (DAP-150) (p150-glued)
          Length = 1300

 Score = 69.7 bits (169), Expect = 1e-11
 Identities = 27/61 (44%), Positives = 41/61 (67%)
 Frame = +3

Query: 477 EVRVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIK 656
           ++ + + S +   ++Y+G T F PG WVG+  DEPSGKN+G+V G+ YF C   YG F++
Sbjct: 11  KIELADGSGRTAFVRYVGETAFAPGTWVGIELDEPSGKNDGSVQGERYFNCEMGYGMFVR 70

Query: 657 P 659
           P
Sbjct: 71  P 71
>sp|Q9VJE5|CL190_DROME Restin homolog (Cytoplasmic linker protein 190) (Microtubule
           binding protein 190) (d-CLIP-190)
          Length = 1690

 Score = 69.3 bits (168), Expect = 1e-11
 Identities = 29/51 (56%), Positives = 36/51 (70%)
 Frame = +3

Query: 501 AKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFI 653
           ++ G ++Y+G T F PG W GV  DEPSGKN+GTVD   YFEC  KYG F+
Sbjct: 251 SRPGILRYLGETQFAPGNWCGVELDEPSGKNDGTVDDIRYFECKPKYGVFV 301

 Score = 65.1 bits (157), Expect = 2e-10
 Identities = 29/54 (53%), Positives = 35/54 (64%)
 Frame = +3

Query: 510 GQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHIT 671
           GQI +IG THF  G W GV  DEP+GKN+G V GK YF+C  K G F +   +T
Sbjct: 137 GQIAFIGDTHFAAGEWAGVVLDEPNGKNDGCVSGKRYFQCEPKRGIFSRLTRLT 190
>sp|Q8CI96|RSNL2_MOUSE Restin-like protein 2
          Length = 704

 Score = 67.4 bits (163), Expect = 5e-11
 Identities = 26/53 (49%), Positives = 35/53 (66%)
 Frame = +3

Query: 510 GQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHI 668
           G IK+ GTT+F PGYW G+  ++P GKN+G+V G  YF C  +YG F  P  +
Sbjct: 498 GTIKFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRV 550

 Score = 62.4 bits (150), Expect = 2e-09
 Identities = 29/65 (44%), Positives = 37/65 (56%)
 Frame = +3

Query: 456 LKISDRCEVRVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPE 635
           LK+ DR  +       K G +++ GTT F  G W G+  DEP GKNNG+V    YF+C  
Sbjct: 283 LKLGDRVVIA----GQKVGTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGRVQYFKCAP 338

Query: 636 KYGAF 650
           KYG F
Sbjct: 339 KYGIF 343

 Score = 54.3 bits (129), Expect = 4e-07
 Identities = 21/58 (36%), Positives = 31/58 (53%)
 Frame = +3

Query: 498 SAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHIT 671
           S +   ++Y+G T F  G W+G+      GKN+G V  K YF C   YG  ++P  +T
Sbjct: 633 SNEMATVRYVGPTDFASGIWLGLELRSAKGKNDGAVGDKRYFTCKPNYGVLVRPSRVT 690
>sp|Q8N3C7|RSNL2_HUMAN Restin-like protein 2
          Length = 705

 Score = 67.4 bits (163), Expect = 5e-11
 Identities = 32/80 (40%), Positives = 44/80 (55%), Gaps = 6/80 (7%)
 Frame = +3

Query: 447 AESLKISDRCE------VRVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVD 608
           A S   + RCE       RV     + G I++ GTT+F PGYW G+  ++P GKN+G+V 
Sbjct: 472 ATSTANNSRCEGELRLGERVLVVGQRLGTIRFFGTTNFAPGYWYGIELEKPHGKNDGSVG 531

Query: 609 GKFYFECPEKYGAFIKPQHI 668
           G  YF C  +YG F  P  +
Sbjct: 532 GVQYFSCSPRYGIFAPPSRV 551

 Score = 62.4 bits (150), Expect = 2e-09
 Identities = 29/65 (44%), Positives = 37/65 (56%)
 Frame = +3

Query: 456 LKISDRCEVRVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPE 635
           LK+ DR  +       K G +++ GTT F  G W G+  DEP GKNNG+V    YF+C  
Sbjct: 283 LKLGDRVVIA----GQKVGTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGKVQYFKCAP 338

Query: 636 KYGAF 650
           KYG F
Sbjct: 339 KYGIF 343

 Score = 56.2 bits (134), Expect = 1e-07
 Identities = 24/73 (32%), Positives = 42/73 (57%)
 Frame = +3

Query: 453 SLKISDRCEVRVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECP 632
           S+K+ +  +V + + S + G ++Y+G T F  G W+G+      GKN+G+V  K YF C 
Sbjct: 620 SVKLHEGSQVLLTS-SNEMGTVRYVGPTDFASGIWLGLELRSAKGKNDGSVGDKRYFTCK 678

Query: 633 EKYGAFIKPQHIT 671
             +G  ++P  +T
Sbjct: 679 PNHGVLVRPSRVT 691
>sp|Q66HD5|RSNL2_RAT Restin-like protein 2
          Length = 599

 Score = 67.4 bits (163), Expect = 5e-11
 Identities = 26/53 (49%), Positives = 35/53 (66%)
 Frame = +3

Query: 510 GQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHI 668
           G IK+ GTT+F PGYW G+  ++P GKN+G+V G  YF C  +YG F  P  +
Sbjct: 499 GTIKFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRV 551

 Score = 63.5 bits (153), Expect = 7e-10
 Identities = 31/72 (43%), Positives = 39/72 (54%)
 Frame = +3

Query: 456 LKISDRCEVRVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPE 635
           LK+ DR  +       K G +++ GTT F  G W G+  DEP GKNNG+V    YF+C  
Sbjct: 283 LKLGDRVVIA----GQKVGTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGRVQYFKCAP 338

Query: 636 KYGAFIKPQHIT 671
           KYG F     IT
Sbjct: 339 KYGIFAPLSKIT 350
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 90,032,179
Number of Sequences: 369166
Number of extensions: 1791878
Number of successful extensions: 3904
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3780
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3892
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8486520240
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)