Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_023_F20
(859 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9D1E6|TBCB_MOUSE Tubulin-specific chaperone B (Tubulin ... 206 7e-53
sp|Q5E951|TBCB_BOVIN Tubulin-specific chaperone B (Tubulin ... 204 3e-52
sp|Q99426|TBCB_HUMAN Tubulin-specific chaperone B (Tubulin ... 201 2e-51
sp|Q20728|YXHK_CAEEL Hypothetical protein F53F4.3 in chromo... 176 6e-44
sp|Q10235|ALP11_SCHPO Cell polarity protein alp11 (Altered ... 94 6e-19
sp|Q01397|DYNA_NEUCR Dynactin, 150 kDa isoform (150 kDa dyn... 70 1e-11
sp|Q9VJE5|CL190_DROME Restin homolog (Cytoplasmic linker pr... 69 1e-11
sp|Q8CI96|RSNL2_MOUSE Restin-like protein 2 67 5e-11
sp|Q8N3C7|RSNL2_HUMAN Restin-like protein 2 67 5e-11
sp|Q66HD5|RSNL2_RAT Restin-like protein 2 67 5e-11
>sp|Q9D1E6|TBCB_MOUSE Tubulin-specific chaperone B (Tubulin folding cofactor B)
(Cytoskeleton associated protein 1)
(Cytoskeleton-associated protein CKAPI)
Length = 244
Score = 206 bits (524), Expect = 7e-53
Identities = 105/238 (44%), Positives = 151/238 (63%), Gaps = 3/238 (1%)
Frame = +3
Query: 3 ISLFITNNATSVQCEKRYPCNLTVGEFKAKLELVTGCDHRHVKLQVFTEDDEPFCWLDDN 182
+ +FI+++ S + EKRY +LT+ EFK KLELV G ++L+++ DD+ + LD
Sbjct: 11 VMVFISSSLNSFRSEKRYSRSLTIAEFKCKLELVVGSPASCMELELYGADDKFYSKLDQE 70
Query: 183 MKALGDYPIKDKMRVHATDPN-LKGGEFETLDESQPLYEISFEEYAKREDTVLAFKRRMK 359
LG YP+ D R+H D + ++ GE+E + + + YEIS E Y +R++TV +F +R K
Sbjct: 71 DALLGSYPVDDGCRIHVIDHSGVRLGEYEDVSKVEK-YEISPEAYERRQNTVRSFMKRSK 129
Query: 360 LGQFREVDPXXXXXXXXXXXXXLQDEKDKAESLKISDRCEVRVPNQSAKRGQIKYIGTTH 539
LG + E L +EK +A ++ + RCEVR P+ S +RG + Y+G T
Sbjct: 130 LGPYNE---ELRAQQEAEAAQRLSEEKAQASAISVGSRCEVRAPDHSLRRGTVMYVGLTD 186
Query: 540 FKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHITAGDFPE--LGLDDL 707
FKPGYWVGV YDEP GKN+G+V+GK YFEC KYGAF+KP +T GDFPE GLD++
Sbjct: 187 FKPGYWVGVRYDEPLGKNDGSVNGKRYFECQAKYGAFVKPSAVTVGDFPEEDYGLDEM 244
>sp|Q5E951|TBCB_BOVIN Tubulin-specific chaperone B (Tubulin folding cofactor B)
(Cytoskeleton associated protein 1)
(Cytoskeleton-associated protein CKAPI)
Length = 244
Score = 204 bits (519), Expect = 3e-52
Identities = 102/238 (42%), Positives = 153/238 (64%), Gaps = 3/238 (1%)
Frame = +3
Query: 3 ISLFITNNATSVQCEKRYPCNLTVGEFKAKLELVTGCDHRHVKLQVFTEDDEPFCWLDDN 182
+++FI+++ S + +KRY +LTV EFK KL+LV G ++L+++ DD+ C LD +
Sbjct: 11 VNVFISSSLNSFRSQKRYSRSLTVAEFKCKLQLVVGSPASCMELELYGPDDKFCCKLDQD 70
Query: 183 MKALGDYPIKDKMRVHATDPN-LKGGEFETLDESQPLYEISFEEYAKREDTVLAFKRRMK 359
LG YP+ D R+H D + + GE+E + + + YEIS E Y +R+D++ +F +R K
Sbjct: 71 DALLGSYPVDDGCRIHVIDHSGARLGEYEDISKVEK-YEISQEAYEQRQDSIRSFLKRNK 129
Query: 360 LGQFREVDPXXXXXXXXXXXXXLQDEKDKAESLKISDRCEVRVPNQSAKRGQIKYIGTTH 539
LG+F E + L +E+ +A ++ + RCEVR P Q +RG + Y+G T
Sbjct: 130 LGRFNEEE---RAQQEAENSQRLIEEEAQASTIPVGSRCEVRTPGQPPRRGTVMYVGLTD 186
Query: 540 FKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHITAGDFPE--LGLDDL 707
FKPGYW+G+ YDEP GKN+G+V+GK YFEC KYGAF+KP +T GDFPE GLD++
Sbjct: 187 FKPGYWIGIRYDEPLGKNDGSVNGKRYFECQAKYGAFVKPSVVTVGDFPEEDYGLDEM 244
>sp|Q99426|TBCB_HUMAN Tubulin-specific chaperone B (Tubulin folding cofactor B)
(Cytoskeleton associated protein 1)
(Cytoskeleton-associated protein CKAPI)
Length = 244
Score = 201 bits (511), Expect = 2e-51
Identities = 103/238 (43%), Positives = 150/238 (63%), Gaps = 3/238 (1%)
Frame = +3
Query: 3 ISLFITNNATSVQCEKRYPCNLTVGEFKAKLELVTGCDHRHVKLQVFTEDDEPFCWLDDN 182
+++FI+++ + + EKRY +LT+ EFK KLEL+ G ++L+++ DD+ + LD
Sbjct: 11 VTVFISSSLNTFRSEKRYSRSLTIAEFKCKLELLVGSPASCMELELYGVDDKFYSKLDQE 70
Query: 183 MKALGDYPIKDKMRVHATDPN-LKGGEFETLDESQPLYEISFEEYAKREDTVLAFKRRMK 359
LG YP+ D R+H D + + GE+E + + Y IS E Y +R+DTV +F +R K
Sbjct: 71 DALLGSYPVDDGCRIHVIDHSGARLGEYEDVSRVEK-YTISQEAYDQRQDTVRSFLKRSK 129
Query: 360 LGQFREVDPXXXXXXXXXXXXXLQDEKDKAESLKISDRCEVRVPNQSAKRGQIKYIGTTH 539
LG++ E + L +EK +A S+ + RCEVR QS +RG + Y+G T
Sbjct: 130 LGRYNEEE---RAQQEAEAAQRLAEEKAQASSIPVGSRCEVRAAGQSPRRGTVMYVGLTD 186
Query: 540 FKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHITAGDFPE--LGLDDL 707
FKPGYW+GV YDEP GKN+G+V+GK YFEC KYGAF+KP +T GDFPE GLD++
Sbjct: 187 FKPGYWIGVRYDEPLGKNDGSVNGKRYFECQAKYGAFVKPAVVTVGDFPEEDYGLDEI 244
>sp|Q20728|YXHK_CAEEL Hypothetical protein F53F4.3 in chromosome V
Length = 229
Score = 176 bits (447), Expect = 6e-44
Identities = 96/239 (40%), Positives = 140/239 (58%), Gaps = 6/239 (2%)
Frame = +3
Query: 9 LFITNNATSVQCEKRYPCNLTVGEFKAKLELVTGCDHRHVKLQVFTEDDEPFCWLDDNMK 188
L IT NAT EK+YP +++ + K KLELV G +++Q+F DD+ L D K
Sbjct: 7 LEITTNATDFPMEKKYPAGMSLNDLKKKLELVVGTTVDSMRIQLFDGDDQLKGELTDGAK 66
Query: 189 ALGDYPIKDKMRVHATDPNLKGGEFETLDESQ-PLYEISFEEYAKREDTVLAFKRRMKLG 365
+L D ++D R+HA D + GG + DES YE+S + Y KR D+V A+K++M+
Sbjct: 67 SLKDLGVRDGYRIHAVD--VTGGNEDFKDESMVEKYEMSDDTYGKRTDSVRAWKKKMQEE 124
Query: 366 QFREVDPXXXXXXXXXXXXXLQDEKDK-----AESLKISDRCEVRVPNQSAKRGQIKYIG 530
Q +++E DK A+++ + +RCEV V Q A+RG++ Y+G
Sbjct: 125 Q--------------GSAAPMENESDKLNEEAAKNIMVGNRCEVTVGAQMARRGEVAYVG 170
Query: 531 TTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHITAGDFPELGLDDL 707
T FK G WVGV YDEP GKN+G+V G YF+C KYG F++P + GDFPEL +D++
Sbjct: 171 ATKFKEGVWVGVKYDEPVGKNDGSVAGVRYFDCDPKYGGFVRPVDVKVGDFPELSIDEI 229
>sp|Q10235|ALP11_SCHPO Cell polarity protein alp11 (Altered polarity protein 11)
Length = 234
Score = 93.6 bits (231), Expect = 6e-19
Identities = 70/241 (29%), Positives = 107/241 (44%), Gaps = 13/241 (5%)
Frame = +3
Query: 3 ISLFITNNATSVQCEKRYPCNLTVGEFKAKLELVTGCDHRHVKL-----------QVFTE 149
I+LFI ++S E+R TV + K KL + G ++ KL VFT
Sbjct: 4 ITLFI--KSSSANAERRINPQWTVSQLKTKLVPIVGTPEQYQKLTYEPASSTVPGHVFTS 61
Query: 150 DDEPFCWLDDNMKALGDYPIKDKMRVHATDPNLKGGEFETLDESQPLYEISFEEYAKRED 329
++E + ++ LG ++D H L + +D+ Y + E+Y R D
Sbjct: 62 EEENLDLGEFKLQPLGTIVVEDTRPPHL---RLDFDDLSQVDK----YVMpreQYENRTD 114
Query: 330 TVLAFKRRMKLGQFREVDPXXXXXXXXXXXXXLQDEKDKAESLKISDRCEVRVPNQSAKR 509
+V A+K+R +LG+F + E K E + + R +
Sbjct: 115 SVYAWKKRNQLGRFNP---------DFEASKASRQESLKRELVDLQKNLNSRCCAAGERY 165
Query: 510 GQIKYIGTTHF--KPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHITAGDF 683
G I+YIG WVGV +DEP GKN+GTV GK YF K+G+F++ + GDF
Sbjct: 166 GTIRYIGLVPEINNDNLWVGVEFDEPVGKNDGTVSGKRYFNAKNKHGSFLRSSEVEVGDF 225
Query: 684 P 686
P
Sbjct: 226 P 226
>sp|Q01397|DYNA_NEUCR Dynactin, 150 kDa isoform (150 kDa dynein-associated polypeptide)
(DP-150) (DAP-150) (p150-glued)
Length = 1300
Score = 69.7 bits (169), Expect = 1e-11
Identities = 27/61 (44%), Positives = 41/61 (67%)
Frame = +3
Query: 477 EVRVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIK 656
++ + + S + ++Y+G T F PG WVG+ DEPSGKN+G+V G+ YF C YG F++
Sbjct: 11 KIELADGSGRTAFVRYVGETAFAPGTWVGIELDEPSGKNDGSVQGERYFNCEMGYGMFVR 70
Query: 657 P 659
P
Sbjct: 71 P 71
>sp|Q9VJE5|CL190_DROME Restin homolog (Cytoplasmic linker protein 190) (Microtubule
binding protein 190) (d-CLIP-190)
Length = 1690
Score = 69.3 bits (168), Expect = 1e-11
Identities = 29/51 (56%), Positives = 36/51 (70%)
Frame = +3
Query: 501 AKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFI 653
++ G ++Y+G T F PG W GV DEPSGKN+GTVD YFEC KYG F+
Sbjct: 251 SRPGILRYLGETQFAPGNWCGVELDEPSGKNDGTVDDIRYFECKPKYGVFV 301
Score = 65.1 bits (157), Expect = 2e-10
Identities = 29/54 (53%), Positives = 35/54 (64%)
Frame = +3
Query: 510 GQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHIT 671
GQI +IG THF G W GV DEP+GKN+G V GK YF+C K G F + +T
Sbjct: 137 GQIAFIGDTHFAAGEWAGVVLDEPNGKNDGCVSGKRYFQCEPKRGIFSRLTRLT 190
>sp|Q8CI96|RSNL2_MOUSE Restin-like protein 2
Length = 704
Score = 67.4 bits (163), Expect = 5e-11
Identities = 26/53 (49%), Positives = 35/53 (66%)
Frame = +3
Query: 510 GQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHI 668
G IK+ GTT+F PGYW G+ ++P GKN+G+V G YF C +YG F P +
Sbjct: 498 GTIKFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRV 550
Score = 62.4 bits (150), Expect = 2e-09
Identities = 29/65 (44%), Positives = 37/65 (56%)
Frame = +3
Query: 456 LKISDRCEVRVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPE 635
LK+ DR + K G +++ GTT F G W G+ DEP GKNNG+V YF+C
Sbjct: 283 LKLGDRVVIA----GQKVGTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGRVQYFKCAP 338
Query: 636 KYGAF 650
KYG F
Sbjct: 339 KYGIF 343
Score = 54.3 bits (129), Expect = 4e-07
Identities = 21/58 (36%), Positives = 31/58 (53%)
Frame = +3
Query: 498 SAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHIT 671
S + ++Y+G T F G W+G+ GKN+G V K YF C YG ++P +T
Sbjct: 633 SNEMATVRYVGPTDFASGIWLGLELRSAKGKNDGAVGDKRYFTCKPNYGVLVRPSRVT 690
>sp|Q8N3C7|RSNL2_HUMAN Restin-like protein 2
Length = 705
Score = 67.4 bits (163), Expect = 5e-11
Identities = 32/80 (40%), Positives = 44/80 (55%), Gaps = 6/80 (7%)
Frame = +3
Query: 447 AESLKISDRCE------VRVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVD 608
A S + RCE RV + G I++ GTT+F PGYW G+ ++P GKN+G+V
Sbjct: 472 ATSTANNSRCEGELRLGERVLVVGQRLGTIRFFGTTNFAPGYWYGIELEKPHGKNDGSVG 531
Query: 609 GKFYFECPEKYGAFIKPQHI 668
G YF C +YG F P +
Sbjct: 532 GVQYFSCSPRYGIFAPPSRV 551
Score = 62.4 bits (150), Expect = 2e-09
Identities = 29/65 (44%), Positives = 37/65 (56%)
Frame = +3
Query: 456 LKISDRCEVRVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPE 635
LK+ DR + K G +++ GTT F G W G+ DEP GKNNG+V YF+C
Sbjct: 283 LKLGDRVVIA----GQKVGTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGKVQYFKCAP 338
Query: 636 KYGAF 650
KYG F
Sbjct: 339 KYGIF 343
Score = 56.2 bits (134), Expect = 1e-07
Identities = 24/73 (32%), Positives = 42/73 (57%)
Frame = +3
Query: 453 SLKISDRCEVRVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECP 632
S+K+ + +V + + S + G ++Y+G T F G W+G+ GKN+G+V K YF C
Sbjct: 620 SVKLHEGSQVLLTS-SNEMGTVRYVGPTDFASGIWLGLELRSAKGKNDGSVGDKRYFTCK 678
Query: 633 EKYGAFIKPQHIT 671
+G ++P +T
Sbjct: 679 PNHGVLVRPSRVT 691
>sp|Q66HD5|RSNL2_RAT Restin-like protein 2
Length = 599
Score = 67.4 bits (163), Expect = 5e-11
Identities = 26/53 (49%), Positives = 35/53 (66%)
Frame = +3
Query: 510 GQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPEKYGAFIKPQHI 668
G IK+ GTT+F PGYW G+ ++P GKN+G+V G YF C +YG F P +
Sbjct: 499 GTIKFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRV 551
Score = 63.5 bits (153), Expect = 7e-10
Identities = 31/72 (43%), Positives = 39/72 (54%)
Frame = +3
Query: 456 LKISDRCEVRVPNQSAKRGQIKYIGTTHFKPGYWVGVHYDEPSGKNNGTVDGKFYFECPE 635
LK+ DR + K G +++ GTT F G W G+ DEP GKNNG+V YF+C
Sbjct: 283 LKLGDRVVIA----GQKVGTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGRVQYFKCAP 338
Query: 636 KYGAFIKPQHIT 671
KYG F IT
Sbjct: 339 KYGIFAPLSKIT 350
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 90,032,179
Number of Sequences: 369166
Number of extensions: 1791878
Number of successful extensions: 3904
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3780
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3892
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8486520240
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)