Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_023_F18
(887 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P16967|SSRA_CANFA Translocon-associated protein alpha su... 153 7e-37
sp|Q9CY50|SSRA_MOUSE Translocon-associated protein alpha su... 153 7e-37
sp|P43307|SSRA_HUMAN Translocon-associated protein alpha su... 152 1e-36
sp|Q5R4X4|SSRA_PONPY Translocon-associated protein alpha su... 151 3e-36
sp|P53815|SSRA_RABIT Translocon-associated protein alpha su... 150 3e-36
sp|Q7TPJ0|SSRA_RAT Translocon-associated protein alpha subu... 143 7e-34
sp|P45433|SSRA_ONCMY Translocon-associated protein alpha su... 142 1e-33
sp|P45434|SSRA_ARATH Translocon-associated protein alpha su... 48 4e-05
sp|P32499|NUP2_YEAST Nucleoporin NUP2 (Nuclear pore protein... 33 1.4
sp|P50898|MIG1_KLULA Regulatory protein MIG1 32 3.1
>sp|P16967|SSRA_CANFA Translocon-associated protein alpha subunit precursor (TRAP-alpha)
(Signal sequence receptor alpha subunit) (SSR-alpha)
(PGP35)
Length = 286
Score = 153 bits (386), Expect = 7e-37
Identities = 96/275 (34%), Positives = 143/275 (52%), Gaps = 5/275 (1%)
Frame = +2
Query: 2 SASDSDEEDIVTDDSAAEEAEDKLNEDKIVTEILDSQQDQTEQTFIDGVPVASKNVQAEL 181
+A D E++ +DS E+ +D+ ++ E D +D+ E+ + G P AS + +
Sbjct: 31 AAQDLTEDEETVEDSIIEDEDDEAEVEE--DEPTDLAEDKEEED-VSGEPEASPSADTTI 87
Query: 182 LFTEPPYNKRSSGVGIELPANKPVKMLISFTNTETETNKDYKLVHVEGFLRYPSDYSYYL 361
LF + G + PAN VK L+ FTN TE D+ + ++ RYP DY +Y+
Sbjct: 88 LFVK----------GEDFPANNIVKFLVGFTNKGTE---DFIVESLDASFRYPQDYQFYI 134
Query: 362 QNFTARPLDITVEPKREATVYYSFTPAQQLAGRPFGLTLRIAYHNNDGKMFTSTFFNDTV 541
QNFTA PL+ V P+R+AT YSF PA+ + GRPFGL + + Y + +G +F FN TV
Sbjct: 135 QNFTALPLNTVVPPQRQATFEYSFIPAEPMGGRPFGLVINLNYKDLNGNVFQDAVFNQTV 194
Query: 542 QVIEIEEGFDTESXXXXXXXXXXXXXXXXXXXHLFTVYALKKFKKSGVSK---QVMELGT 712
+IE E+G D E+ L V L + +S K Q +E+GT
Sbjct: 195 TIIEREDGLDGETIFMYMFLAGLG---------LLVVVGLHQLLESRKRKRPIQKVEMGT 245
Query: 713 SNKADLDLQWIPAEHLKV--RQSPTISPRKRKSRR 811
S++ D+D+ WIP E L + SP PRKR +R
Sbjct: 246 SSQNDVDMSWIPQETLNQINKASPRRLPRKRAQKR 280
>sp|Q9CY50|SSRA_MOUSE Translocon-associated protein alpha subunit precursor (TRAP-alpha)
(Signal sequence receptor alpha subunit) (SSR-alpha)
Length = 286
Score = 153 bits (386), Expect = 7e-37
Identities = 96/274 (35%), Positives = 142/274 (51%), Gaps = 5/274 (1%)
Frame = +2
Query: 5 ASDSDEEDIVTDDSAAEEAEDKLNEDKIVTEILDSQQDQTEQTFIDGVPVASKNVQAELL 184
A D E++ + +DS E+ +D+ ++ E D +D+ E+ + P AS + +L
Sbjct: 32 AQDPTEDEEIVEDSIIEDEDDEAEVEE--DEPTDLAEDKEEED-VSSEPEASPSADTTIL 88
Query: 185 FTEPPYNKRSSGVGIELPANKPVKMLISFTNTETETNKDYKLVHVEGFLRYPSDYSYYLQ 364
F + G + PAN VK L+ FTN TE D+ + ++ RYP DY +Y+Q
Sbjct: 89 FVK----------GEDFPANNIVKFLVGFTNKGTE---DFIVESLDASFRYPQDYQFYIQ 135
Query: 365 NFTARPLDITVEPKREATVYYSFTPAQQLAGRPFGLTLRIAYHNNDGKMFTSTFFNDTVQ 544
NFTA PL+ V P+R+AT YSF PA+ + GRPFGL + + Y + +G +F FN TV
Sbjct: 136 NFTALPLNTVVPPQRQATFEYSFIPAEPMGGRPFGLVINLNYKDLNGNVFQDAVFNQTVT 195
Query: 545 VIEIEEGFDTESXXXXXXXXXXXXXXXXXXXHLFTVYALKKFKKSGVSK---QVMELGTS 715
VIE E+G D E+ L V L + +S K Q +E+GTS
Sbjct: 196 VIEREDGLDGETIFMYMFLAGLG---------LLVVVGLHQLLESRKRKRPIQKVEMGTS 246
Query: 716 NKADLDLQWIPAEHLKV--RQSPTISPRKRKSRR 811
++ D+D+ WIP E L + SP PRKR +R
Sbjct: 247 SQNDVDMSWIPQETLNQINKASPRRQPRKRAQKR 280
>sp|P43307|SSRA_HUMAN Translocon-associated protein alpha subunit precursor (TRAP-alpha)
(Signal sequence receptor alpha subunit) (SSR-alpha)
Length = 286
Score = 152 bits (384), Expect = 1e-36
Identities = 94/271 (34%), Positives = 142/271 (52%), Gaps = 2/271 (0%)
Frame = +2
Query: 5 ASDSDEEDIVTDDSAAEEAEDKLNEDKIVTEILDSQQDQTEQTFIDGVPVASKNVQAELL 184
A D E++ +DS E+ +D+ ++ E D +D+ E+ + G P AS + +L
Sbjct: 32 AQDLTEDEETVEDSIIEDEDDEAEVEE--DEPTDLVEDKEEED-VSGEPEASPSADTTIL 88
Query: 185 FTEPPYNKRSSGVGIELPANKPVKMLISFTNTETETNKDYKLVHVEGFLRYPSDYSYYLQ 364
F + G + PAN VK L+ FTN TE D+ + ++ RYP DY +Y+Q
Sbjct: 89 FVK----------GEDFPANNIVKFLVGFTNKGTE---DFIVESLDASFRYPQDYQFYIQ 135
Query: 365 NFTARPLDITVEPKREATVYYSFTPAQQLAGRPFGLTLRIAYHNNDGKMFTSTFFNDTVQ 544
NFTA PL+ V P+R+AT YSF PA+ + GRPFGL + + Y + +G +F FN TV
Sbjct: 136 NFTALPLNTVVPPQRQATFEYSFIPAEPMGGRPFGLVINLNYKDLNGNVFQDAVFNQTVT 195
Query: 545 VIEIEEGFDTESXXXXXXXXXXXXXXXXXXXHLFTVYALKKFKKSGVSKQVMELGTSNKA 724
VIE E+G D E+ + ++ L + +K Q +E+GTS++
Sbjct: 196 VIEREDGLDGETIFMYMFLAGLGLLV------IVGLHQLLESRKRKRPIQKVEMGTSSQN 249
Query: 725 DLDLQWIPAEHLKV--RQSPTISPRKRKSRR 811
D+D+ WIP E L + SP PRKR +R
Sbjct: 250 DVDMSWIPQETLNQINKASPRRLPRKRAQKR 280
>sp|Q5R4X4|SSRA_PONPY Translocon-associated protein alpha subunit precursor (TRAP-alpha)
(Signal sequence receptor alpha subunit) (SSR-alpha)
Length = 291
Score = 151 bits (381), Expect = 3e-36
Identities = 94/276 (34%), Positives = 143/276 (51%), Gaps = 7/276 (2%)
Frame = +2
Query: 5 ASDSDEEDIVTDDSAAEEAEDKLNEDKIVTEILDSQQDQTEQTFIDGVPVASKNVQAELL 184
A D E++ +DS E+ +D+ ++ E D +D+ E+ + G P AS + +L
Sbjct: 32 AQDLTEDEETVEDSIIEDEDDEAEVEE--DEPTDLVEDKEEED-VSGEPEASPSADTTIL 88
Query: 185 FTEPPYNKRSSGVGIELPANKPVKMLISFTNTETETNKDYKLVHVEGFLRYPSDYSYYLQ 364
F + G + PAN VK L+ FTN TE D+ + ++ RYP DY +Y+Q
Sbjct: 89 FVK----------GEDFPANNIVKFLVGFTNKGTE---DFIVESLDASFRYPQDYQFYIQ 135
Query: 365 NFTARPLDITVEPKREATVYYSFTPAQQLAGRPFGLTLRIAYHNNDGKMFTSTFFNDTVQ 544
NFTA PL+ V P+R+AT YSF PA+ + GRPFGL + + Y + +G +F FN TV
Sbjct: 136 NFTALPLNTVVPPQRQATFEYSFIPAEPMGGRPFGLVINLNYKDLNGNVFQDAVFNQTVT 195
Query: 545 VIEIEEGFDTESXXXXXXXXXXXXXXXXXXXHLFTVYALKKFKKSGVSKQVMELGTSNKA 724
VIE E+G D E+ + ++ L + +K Q +E+GTS++
Sbjct: 196 VIEREDGLDGETIFMYMFLAGLGLLV------IVGLHQLLESRKRKRPVQKVEMGTSSQN 249
Query: 725 DLDLQWIPAEHL-------KVRQSPTISPRKRKSRR 811
D+D+ WIP E L + + SP PRKR +R
Sbjct: 250 DVDMSWIPQETLNQIMQSRRDKASPRRLPRKRAQKR 285
>sp|P53815|SSRA_RABIT Translocon-associated protein alpha subunit precursor (TRAP-alpha)
(Signal sequence receptor alpha subunit) (SSR-alpha)
Length = 286
Score = 150 bits (380), Expect = 3e-36
Identities = 95/274 (34%), Positives = 142/274 (51%), Gaps = 5/274 (1%)
Frame = +2
Query: 5 ASDSDEEDIVTDDSAAEEAEDKLNEDKIVTEILDSQQDQTEQTFIDGVPVASKNVQAELL 184
A D E++ +DS E+ +D+ ++ E D +D+ E+ + G P AS + +L
Sbjct: 32 AQDLSEDEETVEDSVIEDEDDEAEVEE--DEPTDLAEDREEED-VSGEPEASPSADTTIL 88
Query: 185 FTEPPYNKRSSGVGIELPANKPVKMLISFTNTETETNKDYKLVHVEGFLRYPSDYSYYLQ 364
F + G + PAN V+ L+ FTN TE D+ + ++ RYP DY +Y+Q
Sbjct: 89 FVK----------GEDFPANNIVRFLVGFTNKGTE---DFIVESLDASFRYPQDYQFYIQ 135
Query: 365 NFTARPLDITVEPKREATVYYSFTPAQQLAGRPFGLTLRIAYHNNDGKMFTSTFFNDTVQ 544
NFTA PL+ V P+R+AT YSF PA+ + GRPFGL + + Y + +G +F FN TV
Sbjct: 136 NFTALPLNTIVPPQRQATFEYSFIPAEPMGGRPFGLVINLNYKDFNGNVFQDAVFNQTVT 195
Query: 545 VIEIEEGFDTESXXXXXXXXXXXXXXXXXXXHLFTVYALKKFKKSGVSK---QVMELGTS 715
VIE E+G D ++ L V L + +S K Q +E+GTS
Sbjct: 196 VIEREDGLDGQTIFMYMSLAGLG---------LLVVVGLHQLLESRNRKRPIQKVEMGTS 246
Query: 716 NKADLDLQWIPAEHLKV--RQSPTISPRKRKSRR 811
++ D+D+ WIP E L + SP PRKR +R
Sbjct: 247 SQNDVDMSWIPQETLNQINKASPRRLPRKRPQKR 280
>sp|Q7TPJ0|SSRA_RAT Translocon-associated protein alpha subunit precursor (TRAP-alpha)
(Signal sequence receptor alpha subunit) (SSR-alpha)
(Liver regeneration-related protein LRRG137)
Length = 319
Score = 143 bits (360), Expect = 7e-34
Identities = 87/255 (34%), Positives = 131/255 (51%), Gaps = 3/255 (1%)
Frame = +2
Query: 5 ASDSDEEDIVTDDSAAEEAEDKLNEDKIVTEILDSQQDQTEQTFIDGVPVASKNVQAELL 184
A D E++ +D E+ +D+ ++ E D +++ E+ + P AS + +L
Sbjct: 32 AQDLTEDEETVEDPIIEDEDDEAEVEE--DEPTDLAEEKEEEEDVSSEPEASPSADTTIL 89
Query: 185 FTEPPYNKRSSGVGIELPANKPVKMLISFTNTETETNKDYKLVHVEGFLRYPSDYSYYLQ 364
F + G + PAN VK L+ FTN TE D+ + ++ RYP DY +Y+Q
Sbjct: 90 FVK----------GEDFPANNIVKFLVGFTNKGTE---DFIVESLDASFRYPQDYQFYIQ 136
Query: 365 NFTARPLDITVEPKREATVYYSFTPAQQLAGRPFGLTLRIAYHNNDGKMFTSTFFNDTVQ 544
NFTA PL+ V P+R+AT YSF PA+ + GRPFGL + + Y + +G +F FN TV
Sbjct: 137 NFTALPLNTIVPPQRQATFEYSFIPAEPMGGRPFGLVINLNYKDLNGNVFQDAVFNQTVT 196
Query: 545 VIEIEEGFDTESXXXXXXXXXXXXXXXXXXXHLFTVYALKKFKKSGVSK---QVMELGTS 715
VIE E+G D E+ L V L + +S K Q +E+GTS
Sbjct: 197 VIEREDGLDGETIFMYMFLAGLG---------LLVVVGLHQLLESRKRKRPIQKVEMGTS 247
Query: 716 NKADLDLQWIPAEHL 760
++ D+D+ WIP E L
Sbjct: 248 SQNDVDMSWIPQETL 262
>sp|P45433|SSRA_ONCMY Translocon-associated protein alpha subunit precursor (TRAP-alpha)
(Signal sequence receptor alpha subunit) (SSR-alpha)
Length = 288
Score = 142 bits (359), Expect = 1e-33
Identities = 89/277 (32%), Positives = 133/277 (48%), Gaps = 8/277 (2%)
Frame = +2
Query: 2 SASDSDEEDIVTDDSAAEEAEDKLNEDKIVTEILDSQQDQTEQTFIDGVPVAS----KNV 169
SA EDI D + EE E++ ED+++ E +TE + V +
Sbjct: 27 SADSESAEDIFPDSTVDEEEEEE--EDEVLVEEDQVPGSETEDDIDEDAAVGDVTSHPDA 84
Query: 170 QAELLFTEPPYNKRSSGVGIELPANKPVKMLISFTNTETETNKDYKLVHVEGFLRYPSDY 349
++F G E PAN+ VK L+ FTN + ++D+ + +E RYP D+
Sbjct: 85 DTTIVFV----------TGEEFPANEIVKFLVGFTN---KGSQDFTVHSLEASFRYPQDF 131
Query: 350 SYYLQNFTARPLDITVEPKREATVYYSFTPAQQLAGRPFGLTLRIAYHNNDGKMFTSTFF 529
+Y+QNFTA PL V+P+++A+ YSF PAQ +AGRPFGL + + Y +++G F + +
Sbjct: 132 QFYIQNFTALPLSTVVQPQKQASFEYSFIPAQPMAGRPFGLVILLNYQDSEGNGFQTAIY 191
Query: 530 NDTVQVIEIEEGFDTESXXXXXXXXXXXXXXXXXXXHLFTVYALKKFKKSGVSKQVMELG 709
N TV ++E+EEG D E+ + K+F +E G
Sbjct: 192 NQTVTIVELEEGLDGETIFMYIFLTGLVVLAVFGMYQVLESRTRKRFPVK------VETG 245
Query: 710 TSNKADLDLQWIPAEHL----KVRQSPTISPRKRKSR 808
T +D+ WIP E L K SP SPRKR R
Sbjct: 246 TGGMNGVDISWIPQETLNIMSKASASPKASPRKRTKR 282
>sp|P45434|SSRA_ARATH Translocon-associated protein alpha subunit precursor (TRAP-alpha)
(Signal sequence receptor alpha subunit) (SSR-alpha)
Length = 258
Score = 47.8 bits (112), Expect = 4e-05
Identities = 46/185 (24%), Positives = 79/185 (42%), Gaps = 3/185 (1%)
Frame = +2
Query: 8 SDSDEEDIVTDDSAAEEAEDKLNEDKIVTEILDSQQDQTEQTF--IDGVPVASKNVQAEL 181
SD+++ + DD E +D + ED D D +F ++ V V KN A+L
Sbjct: 26 SDAEDHSSLVDDVVGENTDDAVEED-------DHDLDMNLSSFPGVETVCVFPKN-SAKL 77
Query: 182 LFTEPPYNKRSSGVGIELPANKPVKMLISFTNTETETNKDYKLVHVEGFLRYPSDYSYYL 361
+ PA + ++L+ N E ++ + + P D+ +
Sbjct: 78 V-----------------PAGEETELLVGLKN---EGKTRVGVMGIRASVHLPYDHKLLV 117
Query: 362 QNFTARPLD-ITVEPKREATVYYSFTPAQQLAGRPFGLTLRIAYHNNDGKMFTSTFFNDT 538
QN T L+ ++ +AT Y F +Q L F L I Y + +GK + S F+N T
Sbjct: 118 QNLTMLRLNNASIPTSLQATFPYIFAVSQYLQPGAFDLVGYIIY-DVEGKPYQSVFYNGT 176
Query: 539 VQVIE 553
++V+E
Sbjct: 177 IEVVE 181
>sp|P32499|NUP2_YEAST Nucleoporin NUP2 (Nuclear pore protein NUP2) (p95)
Length = 720
Score = 32.7 bits (73), Expect = 1.4
Identities = 30/102 (29%), Positives = 51/102 (50%), Gaps = 11/102 (10%)
Frame = +2
Query: 35 TDDSAAEEAEDKLNEDKIVTEILDSQQ--DQTEQTFIDGVPVASKNVQAELLFT---EPP 199
T +S E++ D + + + + S+ D ++ +F GVP +SKN ++ +F+ P
Sbjct: 334 TSNSKPEKSSDSNDSNPSFSFSIPSKNTPDASKPSFSFGVPNSSKNETSKPVFSFGAATP 393
Query: 200 YNKRSS----GVGIELPANKPVKMLISFTNT--ETETNKDYK 307
K +S +E P++KP LIS T E E+ KD K
Sbjct: 394 SAKEASQEDDNNNVEKPSSKPAFNLISNAGTEKEKESKKDSK 435
>sp|P50898|MIG1_KLULA Regulatory protein MIG1
Length = 474
Score = 31.6 bits (70), Expect = 3.1
Identities = 21/80 (26%), Positives = 36/80 (45%), Gaps = 8/80 (10%)
Frame = +2
Query: 104 DSQQDQTEQTFIDGVPVASKNVQAELLFTEPPYNKRSSGVGIELPANKPVKMLIS----- 268
+S + + FID +P+ S + L FT N SSG+ + PA++ +S
Sbjct: 199 ESDKASSSFKFIDKLPLTSSSSSPSLSFTSHSINNSSSGLLLPRPASRAKLSALSSLQRM 258
Query: 269 ---FTNTETETNKDYKLVHV 319
N+E+ + LVH+
Sbjct: 259 TPLSQNSESYNHSQQNLVHL 278
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 89,287,248
Number of Sequences: 369166
Number of extensions: 1690450
Number of successful extensions: 5101
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4659
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5022
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8886314050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)