Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_023_E11
(783 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q63083|NUCB1_RAT Nucleobindin 1 precursor (CALNUC) (Bone... 132 1e-30
sp|Q02819|NUCB1_MOUSE Nucleobindin 1 precursor (CALNUC) 131 2e-30
sp|Q02818|NUCB1_HUMAN Nucleobindin 1 precursor (CALNUC) 130 4e-30
sp|P80303|NUCB2_HUMAN Nucleobindin 2 precursor (DNA-binding... 129 9e-30
sp|P81117|NUCB2_MOUSE Nucleobindin 2 precursor (DNA-binding... 127 3e-29
sp|Q9JI85|NUCB2_RAT Nucleobindin 2 precursor (DNA-binding p... 126 6e-29
sp|P39931|SS120_YEAST Protein SSP120 precursor 42 0.002
sp|Q25410|MIPR_LYMST Putative molluscan insulin-related pep... 37 0.047
sp|Q9Y6T7|DGKB_HUMAN Diacylglycerol kinase, beta (Diglyceri... 37 0.047
sp|P49621|DGKB_RAT Diacylglycerol kinase, beta (Diglyceride... 37 0.061
>sp|Q63083|NUCB1_RAT Nucleobindin 1 precursor (CALNUC) (Bone 63 kDa calcium-binding
protein)
Length = 459
Score = 132 bits (331), Expect = 1e-30
Identities = 69/165 (41%), Positives = 104/165 (63%), Gaps = 1/165 (0%)
Frame = +1
Query: 115 EDAQNKWLEEQKTKHSQHKAINEPASIEQLKTVWQYDDRMNEDSFDPRTFFYLHDVNGDG 294
++A+ K L+EQ+ +H +H +N P S QLK VW+ D ++ + F+P+TFF LHD+N DG
Sbjct: 199 KEAERK-LQEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDG 257
Query: 295 NLDVAELETLMQRELDKAYDPNDNATDVIERYHEMSRMRKKFLTHVDLDQNHLISLEEFM 474
LD ELE L +EL+K YDP + D+ E E RMR+ + +VD +Q+ L++LEEF+
Sbjct: 258 VLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFL 317
Query: 475 QWTKSGEANEKKE-WKTQEEMDPNYSKEEFDKYYQDYIRRHPELH 606
T+ E E E WKT EM P Y++EE ++ ++ R EL+
Sbjct: 318 ASTQRKEFGETAEGWKT-VEMYPAYTEEELKRFEEELAAREAELN 361
>sp|Q02819|NUCB1_MOUSE Nucleobindin 1 precursor (CALNUC)
Length = 459
Score = 131 bits (330), Expect = 2e-30
Identities = 68/165 (41%), Positives = 104/165 (63%), Gaps = 1/165 (0%)
Frame = +1
Query: 115 EDAQNKWLEEQKTKHSQHKAINEPASIEQLKTVWQYDDRMNEDSFDPRTFFYLHDVNGDG 294
++A+ K L+EQ+ +H +H +N P S QLK VW+ D ++ + F+P+TFF LHD+N DG
Sbjct: 199 KEAERK-LQEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDG 257
Query: 295 NLDVAELETLMQRELDKAYDPNDNATDVIERYHEMSRMRKKFLTHVDLDQNHLISLEEFM 474
LD ELE L +EL+K YDP + D+ E E RMR+ + +VD +Q+ L++LEEF+
Sbjct: 258 VLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFL 317
Query: 475 QWTKSGEANEKKE-WKTQEEMDPNYSKEEFDKYYQDYIRRHPELH 606
T+ E + E WKT EM P Y++EE ++ ++ R EL+
Sbjct: 318 ASTQRKEFGDTGEGWKT-VEMSPAYTEEELKRFEEELAAREAELN 361
>sp|Q02818|NUCB1_HUMAN Nucleobindin 1 precursor (CALNUC)
Length = 461
Score = 130 bits (327), Expect = 4e-30
Identities = 66/164 (40%), Positives = 101/164 (61%)
Frame = +1
Query: 115 EDAQNKWLEEQKTKHSQHKAINEPASIEQLKTVWQYDDRMNEDSFDPRTFFYLHDVNGDG 294
++A+ K LEEQ+ +H +H +N P S QLK VW+ D ++ + F+P+TFF LHD+N DG
Sbjct: 200 KEAERK-LEEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDG 258
Query: 295 NLDVAELETLMQRELDKAYDPNDNATDVIERYHEMSRMRKKFLTHVDLDQNHLISLEEFM 474
LD ELE L +EL+K YDP + D+ E E RMR+ + +VD +Q+ L++LEEF+
Sbjct: 259 VLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFL 318
Query: 475 QWTKSGEANEKKEWKTQEEMDPNYSKEEFDKYYQDYIRRHPELH 606
T+ E + E EM P Y++EE ++ ++ R EL+
Sbjct: 319 ASTQRKEFGDTGEGWETVEMHPAYTEEELRRFEEELAAREAELN 362
>sp|P80303|NUCB2_HUMAN Nucleobindin 2 precursor (DNA-binding protein NEFA) (Gastric cancer
antigen Zg4)
Length = 420
Score = 129 bits (324), Expect = 9e-30
Identities = 64/160 (40%), Positives = 96/160 (60%)
Frame = +1
Query: 91 TFSDAKLLEDAQNKWLEEQKTKHSQHKAINEPASIEQLKTVWQYDDRMNEDSFDPRTFFY 270
T ++ K E+ EE K KH H +N P S +QLK VW+ D ++ + FDP+TFF
Sbjct: 194 TLNEEKRKEEESK--FEEMKKKHENHPKVNHPGSKDQLKEVWEETDGLDPNDFDPKTFFK 251
Query: 271 LHDVNGDGNLDVAELETLMQRELDKAYDPNDNATDVIERYHEMSRMRKKFLTHVDLDQNH 450
LHDVN DG LD ELE L +EL+K YDP + D++E E RMR+ + VD +++
Sbjct: 252 LHDVNSDGFLDEQELEALFTKELEKVYDPKNEEDDMVEMEEERLRMREHVMNEVDTNKDR 311
Query: 451 LISLEEFMQWTKSGEANEKKEWKTQEEMDPNYSKEEFDKY 570
L++LEEF++ T+ E E W+T ++ +++EE +Y
Sbjct: 312 LVTLEEFLKATEKKEFLEPDSWETLDQQQ-FFTEEELKEY 350
>sp|P81117|NUCB2_MOUSE Nucleobindin 2 precursor (DNA-binding protein NEFA)
Length = 420
Score = 127 bits (320), Expect = 3e-29
Identities = 69/187 (36%), Positives = 104/187 (55%)
Frame = +1
Query: 10 QIDRKNDESYKLHXXXXXXXXXXXXXXTFSDAKLLEDAQNKWLEEQKTKHSQHKAINEPA 189
Q DR E +K + T S+ K E+ EE K KH H +N P
Sbjct: 168 QYDRTRHEEFKKYEMMKEHERREYLK-TLSEEKRKEEESK--FEEMKRKHEDHPKVNHPG 224
Query: 190 SIEQLKTVWQYDDRMNEDSFDPRTFFYLHDVNGDGNLDVAELETLMQRELDKAYDPNDNA 369
S +QLK VW+ D ++ + FDP+TFF LHDVN DG LD ELE L REL+K Y+P +
Sbjct: 225 SKDQLKEVWEETDGLDPNDFDPKTFFKLHDVNNDGFLDEQELEALFTRELEKVYNPQNAE 284
Query: 370 TDVIERYHEMSRMRKKFLTHVDLDQNHLISLEEFMQWTKSGEANEKKEWKTQEEMDPNYS 549
D+IE E RMR+ ++ +D +++ L++LEEF++ T+ E E W+T ++ ++
Sbjct: 285 DDMIEMEEERLRMREHVMSEIDNNKDRLVTLEEFLRATEKKEFLEPDSWETLDQQQ-LFT 343
Query: 550 KEEFDKY 570
++E +Y
Sbjct: 344 EDELKEY 350
>sp|Q9JI85|NUCB2_RAT Nucleobindin 2 precursor (DNA-binding protein NEFA)
Length = 420
Score = 126 bits (317), Expect = 6e-29
Identities = 67/187 (35%), Positives = 102/187 (54%)
Frame = +1
Query: 10 QIDRKNDESYKLHXXXXXXXXXXXXXXTFSDAKLLEDAQNKWLEEQKTKHSQHKAINEPA 189
Q DR E +K + + + E+A+ E K KH H +N P
Sbjct: 168 QYDRTRHEEFKKYEMMKEHERREYLKTLSEEKRKEEEAK---FAEMKRKHEDHPKVNHPG 224
Query: 190 SIEQLKTVWQYDDRMNEDSFDPRTFFYLHDVNGDGNLDVAELETLMQRELDKAYDPNDNA 369
S +QLK VW+ D ++ + FDP+TFF LHDVN DG LD ELE L +ELDK Y+P +
Sbjct: 225 SKDQLKEVWEETDGLDPNDFDPKTFFKLHDVNNDGFLDEQELEALFTKELDKVYNPQNAE 284
Query: 370 TDVIERYHEMSRMRKKFLTHVDLDQNHLISLEEFMQWTKSGEANEKKEWKTQEEMDPNYS 549
D+IE E RMR+ + +D +++ L++LEEF++ T+ E E W+T ++ ++
Sbjct: 285 DDMIEMEEERLRMREHVMNEIDNNKDRLVTLEEFLRATEKKEFLEPDSWETLDQQQ-LFT 343
Query: 550 KEEFDKY 570
+EE +Y
Sbjct: 344 EEELKEY 350
>sp|P39931|SS120_YEAST Protein SSP120 precursor
Length = 234
Score = 42.0 bits (97), Expect = 0.002
Identities = 29/97 (29%), Positives = 46/97 (47%), Gaps = 4/97 (4%)
Frame = +1
Query: 214 WQYDDRMNEDSFDPRTFFYLHDVNGDGNL---DVAELETLMQRELDKAYDPNDNATDVIE 384
W D + P TFF LHD+ G L D+ L L + E+ A D + +
Sbjct: 44 WHMDHEHQLKDYTPETFFALHDIKKKGFLDENDILSLYGLNREEIVGAGDGMGQHDESEK 103
Query: 385 RYHEMS-RMRKKFLTHVDLDQNHLISLEEFMQWTKSG 492
+EM+ R+ + +D+D N I+ EE++Q+ K G
Sbjct: 104 IDNEMAKRVVSLIMRLLDVDDNTKITKEEYLQFAKRG 140
>sp|Q25410|MIPR_LYMST Putative molluscan insulin-related peptide(s) receptor precursor
[Contains: Putative molluscan insulin-related peptide(s)
receptor alpha chain; Putative molluscan insulin-related
peptide(s) receptor beta chain]
Length = 1607
Score = 37.4 bits (85), Expect = 0.047
Identities = 26/89 (29%), Positives = 39/89 (43%), Gaps = 9/89 (10%)
Frame = +1
Query: 355 PNDNATDVIERYHEMSRMRKKF---------LTHVDLDQNHLISLEEFMQWTKSGEANEK 507
PN N T YH++ K + L H + I EE + S +N
Sbjct: 660 PNGNITHYKIVYHKLELNEKSYEQRNYCRDPLVHQKKKEKVKIE-EEGKKINNSANSNCC 718
Query: 508 KEWKTQEEMDPNYSKEEFDKYYQDYIRRH 594
K K++EEMD K E + Y++DY+ +H
Sbjct: 719 KCPKSKEEMDTESRKREIEMYFEDYLHKH 747
>sp|Q9Y6T7|DGKB_HUMAN Diacylglycerol kinase, beta (Diglyceride kinase) (DGK-beta) (DAG
kinase beta) (90 kDa diacylglycerol kinase)
Length = 804
Score = 37.4 bits (85), Expect = 0.047
Identities = 22/71 (30%), Positives = 39/71 (54%)
Frame = +1
Query: 265 FYLHDVNGDGNLDVAELETLMQRELDKAYDPNDNATDVIERYHEMSRMRKKFLTHVDLDQ 444
F L+D +G+G LD +ELE ++ + + A + T++ HEM + +D D
Sbjct: 158 FRLYDTDGNGFLDSSELENIISQMMHVAEYLEWDVTELNPILHEM-------MEEIDYDH 210
Query: 445 NHLISLEEFMQ 477
+ +SLEE++Q
Sbjct: 211 DGTVSLEEWIQ 221
>sp|P49621|DGKB_RAT Diacylglycerol kinase, beta (Diglyceride kinase) (DGK-beta) (DAG
kinase beta) (90 kDa diacylglycerol kinase)
Length = 801
Score = 37.0 bits (84), Expect = 0.061
Identities = 22/71 (30%), Positives = 40/71 (56%)
Frame = +1
Query: 265 FYLHDVNGDGNLDVAELETLMQRELDKAYDPNDNATDVIERYHEMSRMRKKFLTHVDLDQ 444
F L+D +G+G LD +ELE ++ + + A + T++ HEM + +D D+
Sbjct: 157 FRLYDTDGNGFLDSSELENIIGQMMHVAEYLEWDVTELNPILHEM-------MEEIDYDR 209
Query: 445 NHLISLEEFMQ 477
+ +SLEE++Q
Sbjct: 210 DGTVSLEEWIQ 220
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 74,278,898
Number of Sequences: 369166
Number of extensions: 1345098
Number of successful extensions: 4941
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4684
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4929
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7357347200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)