Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_023_D18
(872 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O60637|TSN3_HUMAN Tetraspanin-3 (Tspan-3) (Transmembrane... 75 2e-13
sp|Q80WR1|TSN18_MOUSE Tetraspanin-18 (Tspan-18) 74 4e-13
sp|Q9QY33|TSN3_MOUSE Tetraspanin-3 (Tspan-3) (Transmembrane... 74 4e-13
sp|Q9QZA6|CD151_RAT Platelet endothelial tetraspan antigen ... 73 1e-12
sp|O60635|TSN1_HUMAN Tetraspanin-1 (Tspan-1) (Tetraspan NET... 72 2e-12
sp|Q61451|CD53_MOUSE Leukocyte surface antigen CD53 (Cell s... 72 2e-12
sp|Q5RC27|TSN1_PONPY Tetraspanin-1 (Tspan-1) 71 3e-12
sp|O35566|C151_MOUSE Platelet-endothelial tetraspan antigen... 71 4e-12
sp|P24485|CD53_RAT Leukocyte surface antigen CD53 (Cell sur... 71 4e-12
sp|P19397|CD53_HUMAN Leukocyte surface antigen CD53 (Cell s... 70 1e-11
>sp|O60637|TSN3_HUMAN Tetraspanin-3 (Tspan-3) (Transmembrane 4 superfamily member 8)
(Tetraspanin TM4-A)
Length = 253
Score = 75.1 bits (183), Expect = 2e-13
Identities = 52/194 (26%), Positives = 93/194 (47%), Gaps = 8/194 (4%)
Frame = +3
Query: 150 VLVAAGILIFLTAIFGCIGTIKESRCFIIMFXXXXXXXXXXXXXSGTLLFIYKKQAESAV 329
V++A G L+F+ + GC TI+ESRC + F L ++Y+ + E+ V
Sbjct: 56 VIIAVGALLFIIGLIGCCATIRESRCGLATFVIILLLVFVTEVVVVVLGYVYRAKVENEV 115
Query: 330 TTHLQKEFTNKYGVD-EPYTRLWDEFQPEHKCCGIDSGADYKSTKWYNSPNQDFHRDGEV 506
+QK + G + + +R D Q + CCGI + +D+++T W+ ++ V
Sbjct: 116 DRSIQKVYKTYNGTNPDAASRAIDYVQRQLHCCGIHNYSDWENTDWFKET-----KNQSV 170
Query: 507 PLSCCTMHGDGHLNVTQCHKS--RPEDQGPYIYNTGCWEIMQKSV-SLVLH--WSSI--A 665
PLSCC + C+ S P D +Y GC ++ K + +++H W+++ A
Sbjct: 171 PLSCC------RETASNCNGSLAHPSD----LYAEGCEALVVKKLQEIMMHVIWAALAFA 220
Query: 666 VFILCGILLFIVIL 707
L G+L ++L
Sbjct: 221 AIQLLGMLCACIVL 234
>sp|Q80WR1|TSN18_MOUSE Tetraspanin-18 (Tspan-18)
Length = 248
Score = 74.3 bits (181), Expect = 4e-13
Identities = 51/237 (21%), Positives = 97/237 (40%), Gaps = 5/237 (2%)
Frame = +3
Query: 24 IFSYSFLVLLSILCG-GFGGWLISQEHWTILLFGTQTIQSMG-WVLVAAGILIFLTAIFG 197
+F ++F V L C G G W++ + T + + G ++++A G L+FL G
Sbjct: 13 MFVFNFFVFLGGACLLGVGIWVLVDPTGFREIVATNPLLTTGAYIVLAMGGLLFLLGFLG 72
Query: 198 CIGTIKESRCFIIMFXXXXXXXXXXXXXSGTLLFIYKKQAESAVTTHLQKEFTNKYGVD- 374
C G ++E+RC ++ F + L FI++ E KE T Y D
Sbjct: 73 CCGAVRENRCLLLFFFLFILIIFLVELSAAILAFIFR---EHLTREFFTKELTKHYQGDN 129
Query: 375 --EPYTRLWDEFQPEHKCCGIDSGADYKSTKWYNSPNQDFHRDGEVPLSCCTMHGDGHLN 548
+ ++ W+ CCG++ D+K + D EVP +CC
Sbjct: 130 DTDVFSATWNSVMITFGCCGVNGPEDFKLASVFRLLTLDTE---EVPKACCRREPQTRDG 186
Query: 549 VTQCHKSRPEDQGPYIYNTGCWEIMQKSVSLVLHWSSIAVFILCGILLFIVILSFVM 719
V + + P+I GC+ ++ + ++ + + I LF+++ + +
Sbjct: 187 VVLSREECQLGRNPFINKQGCYTVILNTFETYVYLAGAFAIGVLAIELFLMVFAMCL 243
>sp|Q9QY33|TSN3_MOUSE Tetraspanin-3 (Tspan-3) (Transmembrane 4 superfamily member 8)
(Tetraspanin TM4-A) (OSP-associated protein 1) (OAP-1)
Length = 253
Score = 74.3 bits (181), Expect = 4e-13
Identities = 52/194 (26%), Positives = 90/194 (46%), Gaps = 8/194 (4%)
Frame = +3
Query: 150 VLVAAGILIFLTAIFGCIGTIKESRCFIIMFXXXXXXXXXXXXXSGTLLFIYKKQAESAV 329
V++A G L+F+ + GC TI+ESRC + F L ++Y+ + E+ V
Sbjct: 56 VIIAVGALLFIIGLIGCCATIRESRCGLATFVFILLLVFVTEVVVVVLGYVYRAKVENEV 115
Query: 330 TTHLQKEFTNKYGVD-EPYTRLWDEFQPEHKCCGIDSGADYKSTKWYNSPNQDFHRDGEV 506
+QK + G + + +R D Q + CCGI + +D+++T W+ ++ V
Sbjct: 116 DRSIQKVYKTYNGTNSDAASRAIDYVQRQLHCCGIHNYSDWENTDWFKET-----KNQSV 170
Query: 507 PLSCCTMHGDGHLNVTQCHKS--RPEDQGPYIYNTGCWEIMQKSVSLVLH---WSSI--A 665
PLSCC C+ S P D +Y GC ++ K + +L W+++ A
Sbjct: 171 PLSCC------RETAKSCNGSLANPSD----LYAEGCEALVVKKLQEILMHVIWAALAFA 220
Query: 666 VFILCGILLFIVIL 707
L G+L ++L
Sbjct: 221 AIQLLGMLCACIVL 234
>sp|Q9QZA6|CD151_RAT Platelet endothelial tetraspan antigen 3 (CD151 antigen)
Length = 253
Score = 72.8 bits (177), Expect = 1e-12
Identities = 43/171 (25%), Positives = 81/171 (47%), Gaps = 5/171 (2%)
Frame = +3
Query: 24 IFSYS---FLVLLSILCGGFGGWLISQEHWTILLFGTQTIQSMGWVLVAAGILIFLTAIF 194
+F+Y+ +L L+++ G W ++ + I L + T + ++LV AG+++ +T +
Sbjct: 20 LFTYNCCFWLAGLAVMAVGI--WTLALKSDYISLLASSTYLATAYILVVAGVVVMVTGVL 77
Query: 195 GCIGTIKESRCFIIMFXXXXXXXXXXXXXSGTLLFIYKKQAESAVTTHLQKEFTNKY--G 368
GC T KE R + ++ +G L ++Y +Q + + +L+ +Y
Sbjct: 78 GCCATFKERRNLLRLYFILLLIIFLLEIIAGILAYVYYQQLNTELKENLKDTMIKRYHQS 137
Query: 369 VDEPYTRLWDEFQPEHKCCGIDSGADYKSTKWYNSPNQDFHRDGEVPLSCC 521
E T D+ Q E CCG ++ D++ ++W S D VP SCC
Sbjct: 138 GHEGVTNAVDKLQQEFHCCGSNNSRDWRDSEWIRSGEAD---SRVVPDSCC 185
>sp|O60635|TSN1_HUMAN Tetraspanin-1 (Tspan-1) (Tetraspan NET-1) (Tetraspanin TM4-C)
Length = 241
Score = 72.4 bits (176), Expect = 2e-12
Identities = 47/188 (25%), Positives = 77/188 (40%), Gaps = 9/188 (4%)
Frame = +3
Query: 48 LLSILCGG----FGGWLISQEHWTILLFGTQTIQSM-----GWVLVAAGILIFLTAIFGC 200
LL LCG G W+ + +FG + +M G+ L+AAG+++F GC
Sbjct: 16 LLIFLCGAALLAVGIWVSIDGASFLKIFGPLSSSAMQFVNVGYFLIAAGVVVFALGFLGC 75
Query: 201 IGTIKESRCFIIMFXXXXXXXXXXXXXSGTLLFIYKKQAESAVTTHLQKEFTNKYGVDEP 380
G ES+C ++ F + + +Y AE +T + YG E
Sbjct: 76 YGAKTESKCALVTFFFILLLIFIAEVAAAVVALVYTTMAEHFLTLLVVPAIKKDYGSQED 135
Query: 381 YTRLWDEFQPEHKCCGIDSGADYKSTKWYNSPNQDFHRDGEVPLSCCTMHGDGHLNVTQC 560
+T++W+ KCCG + D++ + + F + P CC + N T C
Sbjct: 136 FTQVWNTTMKGLKCCGFTNYTDFEDSPY-------FKENSAFPPFCCNDNVTNTANET-C 187
Query: 561 HKSRPEDQ 584
K + DQ
Sbjct: 188 TKQKAHDQ 195
>sp|Q61451|CD53_MOUSE Leukocyte surface antigen CD53 (Cell surface glycoprotein CD53)
Length = 219
Score = 72.0 bits (175), Expect = 2e-12
Identities = 42/155 (27%), Positives = 75/155 (48%), Gaps = 6/155 (3%)
Frame = +3
Query: 9 LKSINIFSYSFLV--LLSILCG----GFGGWLISQEHWTILLFGTQTIQSMGWVLVAAGI 170
+ S+ + Y + LL +CG GFG + + Q + +L F ++G +LV G
Sbjct: 3 MSSLKLLKYVLFIFNLLFWVCGCCILGFGIYFLVQNTYGVL-FRNLPFLTLGNILVIVGS 61
Query: 171 LIFLTAIFGCIGTIKESRCFIIMFXXXXXXXXXXXXXSGTLLFIYKKQAESAVTTHLQKE 350
+I + A GC+G+IKE++C ++ F LLF+Y+++ + V L
Sbjct: 62 IIMVVAFLGCMGSIKENKCLLMSFFVLLLIILLAEVTIAILLFVYEQKLNTLVAEGLNDS 121
Query: 351 FTNKYGVDEPYTRLWDEFQPEHKCCGIDSGADYKS 455
+ Y D + WD Q + +CCG++ +D+ S
Sbjct: 122 IQH-YHSDNSTMKAWDFIQTQLQCCGVNGSSDWTS 155
>sp|Q5RC27|TSN1_PONPY Tetraspanin-1 (Tspan-1)
Length = 241
Score = 71.2 bits (173), Expect = 3e-12
Identities = 50/188 (26%), Positives = 78/188 (41%), Gaps = 9/188 (4%)
Frame = +3
Query: 48 LLSILCGG----FGGWLISQEHWTILLFGTQTIQSM-----GWVLVAAGILIFLTAIFGC 200
LL LCG G W+ + +FG + +M G+ L+AAG+++F GC
Sbjct: 16 LLIFLCGAALLAVGIWVSIDGASFLKIFGPLSSSAMQFVNVGYFLIAAGVVVFALGFLGC 75
Query: 201 IGTIKESRCFIIMFXXXXXXXXXXXXXSGTLLFIYKKQAESAVTTHLQKEFTNKYGVDEP 380
G ES+C ++ F + + +Y AE +T + YG E
Sbjct: 76 YGAKTESKCALMTFFFILLLIFIAEVAAAVVALVYTTMAEHFLTLLVVPAIKKDYGSQED 135
Query: 381 YTRLWDEFQPEHKCCGIDSGADYKSTKWYNSPNQDFHRDGEVPLSCCTMHGDGHLNVTQC 560
+T++W+ KCCG + D++ + Y N F P CC + N T C
Sbjct: 136 FTQVWNTTMKGLKCCGFTNYTDFEDSP-YLKENHAF------PPFCCNDNITNTANDT-C 187
Query: 561 HKSRPEDQ 584
K + EDQ
Sbjct: 188 TKQKAEDQ 195
>sp|O35566|C151_MOUSE Platelet-endothelial tetraspan antigen 3 (PETA-3) (GP27) (Membrane
glycoprotein SFA-1) (CD151 antigen)
Length = 253
Score = 70.9 bits (172), Expect = 4e-12
Identities = 42/171 (24%), Positives = 81/171 (47%), Gaps = 5/171 (2%)
Frame = +3
Query: 24 IFSYS---FLVLLSILCGGFGGWLISQEHWTILLFGTQTIQSMGWVLVAAGILIFLTAIF 194
+F+Y+ +L L+++ G W ++ + I L + T + ++LV AG+++ +T +
Sbjct: 20 LFTYNCCFWLAGLAVMAVGI--WTLALKSDYISLLASSTYLATAYILVVAGVVVMVTGVL 77
Query: 195 GCIGTIKESRCFIIMFXXXXXXXXXXXXXSGTLLFIYKKQAESAVTTHLQKEFTNKY--G 368
GC T KE R + ++ +G L ++Y +Q + + +L+ +Y
Sbjct: 78 GCCATFKERRNLLRLYFILLLIIFLLEIIAGILAYVYYQQLNTELKENLKDTMVKRYHQS 137
Query: 369 VDEPYTRLWDEFQPEHKCCGIDSGADYKSTKWYNSPNQDFHRDGEVPLSCC 521
E + D+ Q E CCG ++ D++ ++W S D VP SCC
Sbjct: 138 GHEGVSSAVDKLQQEFHCCGSNNSQDWQDSEWIRSGEAD---SRVVPDSCC 185
>sp|P24485|CD53_RAT Leukocyte surface antigen CD53 (Cell surface glycoprotein CD53)
(Leukocyte antigen MRC OX-44)
Length = 219
Score = 70.9 bits (172), Expect = 4e-12
Identities = 44/145 (30%), Positives = 71/145 (48%), Gaps = 1/145 (0%)
Frame = +3
Query: 24 IFSYSFLVLLSILCG-GFGGWLISQEHWTILLFGTQTIQSMGWVLVAAGILIFLTAIFGC 200
+F ++FL + C GFG L+ Q + IL F ++G VLV G +I + A GC
Sbjct: 13 LFFFNFLFWVCGCCILGFGIHLLVQNTYGIL-FRNLPFLTLGNVLVIVGSIIMVVAFLGC 71
Query: 201 IGTIKESRCFIIMFXXXXXXXXXXXXXSGTLLFIYKKQAESAVTTHLQKEFTNKYGVDEP 380
+G+IKE++C ++ F LLF+Y+K+ + V L + Y D
Sbjct: 72 MGSIKENKCLLMSFFVLLLLILLAEVTLAILLFVYEKKINTLVAEGLNDSIQH-YHSDNS 130
Query: 381 YTRLWDEFQPEHKCCGIDSGADYKS 455
WD Q + +CCG++ +D+ S
Sbjct: 131 TRMAWDFIQSQLQCCGVNGSSDWIS 155
>sp|P19397|CD53_HUMAN Leukocyte surface antigen CD53 (Cell surface glycoprotein CD53)
(Tetraspanin-25) (Tspan-25)
Length = 219
Score = 69.7 bits (169), Expect = 1e-11
Identities = 43/155 (27%), Positives = 75/155 (48%), Gaps = 6/155 (3%)
Frame = +3
Query: 9 LKSINIFSYS--FLVLLSILCG----GFGGWLISQEHWTILLFGTQTIQSMGWVLVAAGI 170
+ S+ + Y F LL +CG GFG +L+ ++ +L ++ ++G V V G
Sbjct: 3 MSSLKLLKYVLFFFNLLFWICGCCILGFGIYLLIHNNFGVLFHNLPSL-TLGNVFVIVGS 61
Query: 171 LIFLTAIFGCIGTIKESRCFIIMFXXXXXXXXXXXXXSGTLLFIYKKQAESAVTTHLQKE 350
+I + A GC+G+IKE++C ++ F LLF+Y+++ V L +
Sbjct: 62 IIMVVAFLGCMGSIKENKCLLMSFFILLLIILLAEVTLAILLFVYEQKLNEYVAKGL-TD 120
Query: 351 FTNKYGVDEPYTRLWDEFQPEHKCCGIDSGADYKS 455
++Y D WD Q +CCGI+ +D+ S
Sbjct: 121 SIHRYHSDNSTKAAWDSIQSFLQCCGINGTSDWTS 155
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 98,879,848
Number of Sequences: 369166
Number of extensions: 2106284
Number of successful extensions: 5773
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5537
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5744
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8646143400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)