Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_023_C20
(898 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9Y673|ALG5_HUMAN Dolichyl-phosphate beta-glucosyltransf... 172 9e-43
sp|Q9DB25|ALG5_MOUSE Dolichyl-phosphate beta-glucosyltransf... 171 3e-42
sp|P40350|ALG5_YEAST Dolichyl-phosphate beta-glucosyltransf... 105 2e-22
sp|Q6D2F0|ARNC_ERWCT Undecaprenyl-phosphate 4-deoxy-4-forma... 69 2e-11
sp|Q7N3Q6|ARNC_PHOLL Undecaprenyl-phosphate 4-deoxy-4-forma... 65 3e-10
sp|O60762|DPM1_HUMAN Dolichol-phosphate mannosyltransferase... 64 4e-10
sp|Q9WU83|DPM1_CRIGR Dolichol-phosphate mannosyltransferase... 63 1e-09
sp|O70152|DPM1_MOUSE Dolichol-phosphate mannosyltransferase... 62 2e-09
sp|Q8XDZ5|ARNC_ECO57 Undecaprenyl-phosphate 4-deoxy-4-forma... 62 2e-09
sp|Q8D342|ARNC_WIGBR Undecaprenyl-phosphate 4-deoxy-4-forma... 62 2e-09
>sp|Q9Y673|ALG5_HUMAN Dolichyl-phosphate beta-glucosyltransferase
(DolP-glucosyltransferase)
Length = 324
Score = 172 bits (437), Expect = 9e-43
Identities = 90/164 (54%), Positives = 118/164 (71%), Gaps = 1/164 (0%)
Frame = +3
Query: 33 KVFPRLKDSGTINLSVIIPAYNEETRLPVMIDEALQYLEKC-KTEKSLSYEIIIVDDGSN 209
+ P + DS T LSV++P+YNEE RLPVM+DEAL YLEK K + + +YE+I+VDDGS
Sbjct: 54 ETLPSIWDSPTKQLSVVVPSYNEEKRLPVMMDEALSYLEKRQKRDPAFTYEVIVVDDGSK 113
Query: 210 DRTTDIALEYSQKYTTNVVRVLTLDKNRGKGGAVRLGMLSARGKELLFADADGATLFXXX 389
D+T+ +A +Y QKY ++ VRV+TL KNRGKGGA+R+G+ S+RG+++L ADADGAT F
Sbjct: 114 DQTSKVAFKYCQKYGSDKVRVITLVKNRGKGGAIRMGIFSSRGEKILMADADGATKFPDV 173
Query: 390 XXXXXXXXIQLNRSDCNMALVCGSRAHLEKESIVKRSIFRTILM 521
L MA+ CGSRAHLEKESI +RS FRT+LM
Sbjct: 174 EKLEKGLN-DLQPWPNQMAIACGSRAHLEKESIAQRSYFRTLLM 216
Score = 135 bits (339), Expect = 2e-31
Identities = 60/91 (65%), Positives = 73/91 (80%)
Frame = +2
Query: 521 DTQCGFKLFSRPAARLLFGNLHVERWAFDVDILYLAAHFRIPIGEVCVTWTEKEGSKLVP 700
DTQCGFKLF+R AA F +LHVERWAFDV++LY+A F+IPI E+ V WTE EGSKLVP
Sbjct: 233 DTQCGFKLFTREAASRTFSSLHVERWAFDVELLYIAQFFKIPIAEIAVNWTEIEGSKLVP 292
Query: 701 VFSWLQMAKDILLIRLRYMLGAWKIEPQHKL 793
+SWLQM KD+L IRLRY+ GAW++E K+
Sbjct: 293 FWSWLQMGKDLLFIRLRYLTGAWRLEQTRKM 323
>sp|Q9DB25|ALG5_MOUSE Dolichyl-phosphate beta-glucosyltransferase
(DolP-glucosyltransferase)
Length = 324
Score = 171 bits (433), Expect = 3e-42
Identities = 91/164 (55%), Positives = 118/164 (71%), Gaps = 1/164 (0%)
Frame = +3
Query: 33 KVFPRLKDSGTINLSVIIPAYNEETRLPVMIDEALQYLEKC-KTEKSLSYEIIIVDDGSN 209
+ P + DS T LSV++P+YNEE RLPVM+DEAL YLEK K + + +YE+I+VDDGS
Sbjct: 54 EALPSIWDSPTKQLSVVVPSYNEEKRLPVMMDEALNYLEKRQKHDCTFTYEVIVVDDGSE 113
Query: 210 DRTTDIALEYSQKYTTNVVRVLTLDKNRGKGGAVRLGMLSARGKELLFADADGATLFXXX 389
D+T+ +AL+Y QKY ++ VRV+TL +NRGKGGAVR+G+ S+RG+++L ADADGAT F
Sbjct: 114 DQTSKVALKYCQKYGSDKVRVITLVRNRGKGGAVRMGVFSSRGEKILMADADGATKFPDV 173
Query: 390 XXXXXXXXIQLNRSDCNMALVCGSRAHLEKESIVKRSIFRTILM 521
L MA+ CGSRAHLEKESI +RS FRT LM
Sbjct: 174 EKLEKGLS-DLQPWPEQMAIACGSRAHLEKESIAQRSYFRTFLM 216
Score = 127 bits (318), Expect = 5e-29
Identities = 57/90 (63%), Positives = 70/90 (77%)
Frame = +2
Query: 521 DTQCGFKLFSRPAARLLFGNLHVERWAFDVDILYLAAHFRIPIGEVCVTWTEKEGSKLVP 700
DTQCGFKL +R AA F +LH+ERWAFDV++LY+A +IPI EV V WTE EGSKLVP
Sbjct: 233 DTQCGFKLLTREAAARTFSSLHIERWAFDVELLYIAQCLQIPIAEVAVNWTEIEGSKLVP 292
Query: 701 VFSWLQMAKDILLIRLRYMLGAWKIEPQHK 790
+SWLQM KD+L IRLRY+ GAW+++ K
Sbjct: 293 FWSWLQMGKDLLFIRLRYLTGAWRLKQTRK 322
>sp|P40350|ALG5_YEAST Dolichyl-phosphate beta-glucosyltransferase
(DolP-glucosyltransferase)
Length = 334
Score = 105 bits (261), Expect = 2e-22
Identities = 69/181 (38%), Positives = 100/181 (55%), Gaps = 11/181 (6%)
Frame = +3
Query: 12 DPNEQIYKVFPRL---KDSGTINLSVIIPAYNEETRLPVMIDEALQYLEKCKTEKSLSYE 182
+ ++ + P L +D I LSV+IP+YNE R+ +M+ +A+ +L K + +E
Sbjct: 52 EKGHEVSRALPNLNEHQDDEEIFLSVVIPSYNETGRILLMLTDAISFL---KEKYGSRWE 108
Query: 183 IIIVDDGSNDRTTDIALEYSQ---KYTTNVVRVLTLDKNRGKGGAVRLGMLSARGKELLF 353
I+IVDDGS D TT L+ + K R++ +NRGKGGAVR G L RGK LF
Sbjct: 109 IVIVDDGSTDNTTQYCLKICKEQFKLNYEQFRIIKFSQNRGKGGAVRQGFLHIRGKYGLF 168
Query: 354 ADADGATLF-XXXXXXXXXXXIQLNRSDCNM---ALVCGSRAHL-EKESIVKRSIFRTIL 518
ADADGA+ F I+ + +D A+ GSRAH+ E+++KRS+ R L
Sbjct: 169 ADADGASKFSDVEKLIDAISKIETSSTDLKTTKPAVAIGSRAHMVNTEAVIKRSMIRNCL 228
Query: 519 M 521
M
Sbjct: 229 M 229
Score = 88.6 bits (218), Expect = 2e-17
Identities = 41/84 (48%), Positives = 58/84 (69%)
Frame = +2
Query: 521 DTQCGFKLFSRPAARLLFGNLHVERWAFDVDILYLAAHFRIPIGEVCVTWTEKEGSKLVP 700
DTQCGFKLF+R A +F LH E W FDV+IL LA RI I E+ ++W E +GSK+
Sbjct: 246 DTQCGFKLFNRAAILKIFPYLHTEGWIFDVEILILAIRKRIQIEEIPISWHEVDGSKMAL 305
Query: 701 VFSWLQMAKDILLIRLRYMLGAWK 772
++MAKD+++IR+ Y+LG ++
Sbjct: 306 AIDSIKMAKDLVIIRMAYLLGIYR 329
>sp|Q6D2F0|ARNC_ERWCT Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase
(Undecaprenyl-phosphate Ara4FN transferase) (Ara4FN
transferase)
Length = 327
Score = 68.9 bits (167), Expect = 2e-11
Identities = 39/99 (39%), Positives = 58/99 (58%)
Frame = +3
Query: 69 NLSVIIPAYNEETRLPVMIDEALQYLEKCKTEKSLSYEIIIVDDGSNDRTTDIALEYSQK 248
N+SV+IP YNEE LPV+I+ L K +EII+VDDGSNDR+ ++ E +
Sbjct: 7 NVSVVIPVYNEEESLPVLIERTLAACRKI----GKPWEIILVDDGSNDRSAELLTEAASD 62
Query: 249 YTTNVVRVLTLDKNRGKGGAVRLGMLSARGKELLFADAD 365
+++ VL L++N G+ A+ G A G ++ DAD
Sbjct: 63 PEKHIIAVL-LNRNYGQHSAIMAGFQQAVGDVVITLDAD 100
>sp|Q7N3Q6|ARNC_PHOLL Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase
(Undecaprenyl-phosphate Ara4FN transferase) (Ara4FN
transferase) (Polymyxin resistance protein pmrF)
Length = 325
Score = 65.1 bits (157), Expect = 3e-10
Identities = 32/98 (32%), Positives = 61/98 (62%)
Frame = +3
Query: 72 LSVIIPAYNEETRLPVMIDEALQYLEKCKTEKSLSYEIIIVDDGSNDRTTDIALEYSQKY 251
+SV+IP YNEE LP++++ L ++ E YE+I+VDDGS+D++ +I ++ +++
Sbjct: 9 VSVVIPIYNEEESLPLLLERTLAACKQLTQE----YELILVDDGSSDKSAEILIQAAEQP 64
Query: 252 TTNVVRVLTLDKNRGKGGAVRLGMLSARGKELLFADAD 365
+++ +L L++N G+ A+ G G ++ DAD
Sbjct: 65 ENHIIAIL-LNRNYGQHSAIMAGFNQVNGDLIITLDAD 101
>sp|O60762|DPM1_HUMAN Dolichol-phosphate mannosyltransferase (Dolichol-phosphate mannose
synthase) (Dolichyl-phosphate
beta-D-mannosyltransferase) (Mannose-P-dolichol
synthase) (MPD synthase) (DPM synthase)
Length = 260
Score = 64.3 bits (155), Expect = 4e-10
Identities = 36/97 (37%), Positives = 55/97 (56%)
Frame = +3
Query: 75 SVIIPAYNEETRLPVMIDEALQYLEKCKTEKSLSYEIIIVDDGSNDRTTDIALEYSQKYT 254
SV++P YNE LP+++ L K +E ++YEIII+DDGS D T D+A + + Y
Sbjct: 28 SVLLPTYNERENLPLIV----WLLVKSFSESGINYEIIIIDDGSPDGTRDVAEQLEKIYG 83
Query: 255 TNVVRVLTLDKNRGKGGAVRLGMLSARGKELLFADAD 365
++ + + +K G G A GM A G ++ DAD
Sbjct: 84 SDRILLRpreKKLGLGTAYIHGMKHATGNYIIIMDAD 120
>sp|Q9WU83|DPM1_CRIGR Dolichol-phosphate mannosyltransferase (Dolichol-phosphate mannose
synthase) (Dolichyl-phosphate
beta-D-mannosyltransferase) (Mannose-P-dolichol
synthase) (MPD synthase) (DPM synthase)
Length = 266
Score = 62.8 bits (151), Expect = 1e-09
Identities = 37/108 (34%), Positives = 59/108 (54%)
Frame = +3
Query: 42 PRLKDSGTINLSVIIPAYNEETRLPVMIDEALQYLEKCKTEKSLSYEIIIVDDGSNDRTT 221
P+ + S SV++P YNE LP+++ L K +E S++YEIII+DDGS D T
Sbjct: 23 PQGRSSRRDKYSVLLPTYNERENLPLIV----WLLVKSFSESSINYEIIIIDDGSPDGTR 78
Query: 222 DIALEYSQKYTTNVVRVLTLDKNRGKGGAVRLGMLSARGKELLFADAD 365
++A + + Y + + + +K G G A G+ A G ++ DAD
Sbjct: 79 EVAEQLEKIYGPDRILLRpreKKLGLGTAYIHGIKHATGNYVIIMDAD 126
>sp|O70152|DPM1_MOUSE Dolichol-phosphate mannosyltransferase (Dolichol-phosphate mannose
synthase) (Dolichyl-phosphate
beta-D-mannosyltransferase) (Mannose-P-dolichol
synthase) (MPD synthase) (DPM synthase)
Length = 260
Score = 62.4 bits (150), Expect = 2e-09
Identities = 36/108 (33%), Positives = 60/108 (55%)
Frame = +3
Query: 42 PRLKDSGTINLSVIIPAYNEETRLPVMIDEALQYLEKCKTEKSLSYEIIIVDDGSNDRTT 221
P+ + S SV++P YNE LP+++ L K +E +++YEIII+DDGS D T
Sbjct: 17 PQGRSSRQDKYSVLLPTYNERENLPLIV----WLLVKSFSESAINYEIIIIDDGSPDGTR 72
Query: 222 DIALEYSQKYTTNVVRVLTLDKNRGKGGAVRLGMLSARGKELLFADAD 365
++A + ++ Y + + + +K G G A G+ A G ++ DAD
Sbjct: 73 EVAEQLAEIYGPDRILLRpreKKLGLGTAYIHGIKHATGNYVIIMDAD 120
>sp|Q8XDZ5|ARNC_ECO57 Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase
(Undecaprenyl-phosphate Ara4FN transferase) (Ara4FN
transferase)
Length = 322
Score = 62.4 bits (150), Expect = 2e-09
Identities = 35/98 (35%), Positives = 55/98 (56%)
Frame = +3
Query: 72 LSVIIPAYNEETRLPVMIDEALQYLEKCKTEKSLSYEIIIVDDGSNDRTTDIALEYSQKY 251
+SV+IP YNE+ LP +I E E YEI+++DDGS+D + I +E SQ
Sbjct: 10 VSVVIPVYNEQESLPELIRRTTTACESLGKE----YEILLIDDGSSDNSAHILVEASQAE 65
Query: 252 TTNVVRVLTLDKNRGKGGAVRLGMLSARGKELLFADAD 365
+++V +L L++N G+ A+ G G ++ DAD
Sbjct: 66 NSHIVSIL-LNRNYGQHSAIMAGFSHVTGDLIITLDAD 102
>sp|Q8D342|ARNC_WIGBR Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase
(Undecaprenyl-phosphate Ara4FN transferase) (Ara4FN
transferase)
Length = 323
Score = 62.0 bits (149), Expect = 2e-09
Identities = 36/98 (36%), Positives = 55/98 (56%)
Frame = +3
Query: 72 LSVIIPAYNEETRLPVMIDEALQYLEKCKTEKSLSYEIIIVDDGSNDRTTDIALEYSQKY 251
LS+IIP YNE+ L +I + K K + YEIII+DDGSND++ +I + + K
Sbjct: 7 LSIIIPVYNEQDSLIELIKRTVNTCSKLK----IKYEIIIIDDGSNDKSINILEKEALKQ 62
Query: 252 TTNVVRVLTLDKNRGKGGAVRLGMLSARGKELLFADAD 365
+ +V + L KN G+ A+ G + G ++ DAD
Sbjct: 63 NSKIVAIF-LKKNYGQHSAIMAGFKHSSGDLVITMDAD 99
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 101,610,161
Number of Sequences: 369166
Number of extensions: 2071772
Number of successful extensions: 5573
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5328
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5523
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9030416440
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)