Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_023_C11
(465 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q90512|ODO2_FUGRU Dihydrolipoyllysine-residue succinyltr... 91 1e-18
sp|P36957|ODO2_HUMAN Dihydrolipoyllysine-residue succinyltr... 83 3e-16
sp|Q9N0F1|ODO2_PIG Dihydrolipoyllysine-residue succinyltran... 82 6e-16
sp|Q01205|ODO2_RAT Dihydrolipoyllysine-residue succinyltran... 79 4e-15
sp|Q9D2G2|ODO2_MOUSE Dihydrolipoyllysine-residue succinyltr... 78 9e-15
sp|O94681|ODO2_SCHPO Probable dihydrolipoyllysine-residue s... 69 4e-12
sp|P52993|ODO2_RALEU Dihydrolipoyllysine-residue succinyltr... 65 7e-11
sp|P19262|ODO2_YEAST Dihydrolipoyllysine-residue succinyltr... 62 6e-10
sp|P20708|ODO2_AZOVI Dihydrolipoyllysine-residue succinyltr... 61 1e-09
sp|P11961|ODP2_BACST Dihydrolipoyllysine-residue acetyltran... 60 2e-09
>sp|Q90512|ODO2_FUGRU Dihydrolipoyllysine-residue succinyltransferase component of
2-oxoglutarate dehydrogenase complex, mitochondrial
precursor (Dihydrolipoamide succinyltransferase
component of 2-oxoglutarate dehydrogenase complex) (E2)
(E2K)
Length = 409
Score = 90.9 bits (224), Expect = 1e-18
Identities = 51/101 (50%), Positives = 62/101 (61%), Gaps = 1/101 (0%)
Frame = +3
Query: 132 REIGTSLFLFEDKI-IKTPPFAESVTEGDVRWSKKPGDTVVVDEVLGEIETDKTAIPIHS 308
R TS+ +D + +KTP FAESVTEGDVRW K GD+V DEV+ EIETDKT++ + S
Sbjct: 27 RYFRTSVVHRDDLVTVKTPAFAESVTEGDVRWEKAVGDSVTEDEVVCEIETDKTSVQVPS 86
Query: 309 PFQGTILELIVSDGDKVIANQDLVKISLGAVTETKKDVVIE 431
P G I EL+V DG KV L K+ GA E V E
Sbjct: 87 PAAGVIEELLVPDGGKVEGGTPLFKLRKGAAAEAAPSSVTE 127
>sp|P36957|ODO2_HUMAN Dihydrolipoyllysine-residue succinyltransferase component of
2-oxoglutarate dehydrogenase complex, mitochondrial
precursor (Dihydrolipoamide succinyltransferase
component of 2-oxoglutarate dehydrogenase complex) (E2)
(E2K)
Length = 453
Score = 82.8 bits (203), Expect = 3e-16
Identities = 42/83 (50%), Positives = 56/83 (67%)
Frame = +3
Query: 111 NKIYTIKREIGTSLFLFEDKIIKTPPFAESVTEGDVRWSKKPGDTVVVDEVLGEIETDKT 290
N +++++ T++ + +KTP FAESVTEGDVRW K GDTV DEV+ EIETDKT
Sbjct: 52 NSVFSVRFFRTTAVCKDDLVTVKTPAFAESVTEGDVRWEKAVGDTVAEDEVVCEIETDKT 111
Query: 291 AIPIHSPFQGTILELIVSDGDKV 359
++ + SP G I L+V DG KV
Sbjct: 112 SVQVPSPANGVIEALLVPDGGKV 134
>sp|Q9N0F1|ODO2_PIG Dihydrolipoyllysine-residue succinyltransferase component of
2-oxoglutarate dehydrogenase complex, mitochondrial
precursor (Dihydrolipoamide succinyltransferase
component of 2-oxoglutarate dehydrogenase complex) (E2)
(E2K) (E2o) (PE2o)
Length = 455
Score = 82.0 bits (201), Expect = 6e-16
Identities = 40/62 (64%), Positives = 46/62 (74%)
Frame = +3
Query: 174 IKTPPFAESVTEGDVRWSKKPGDTVVVDEVLGEIETDKTAIPIHSPFQGTILELIVSDGD 353
+KTP FAESVTEGDVRW K GDTV DEV+ EIETDKT++ + SP G I L+V DG
Sbjct: 74 VKTPAFAESVTEGDVRWEKAVGDTVAEDEVVCEIETDKTSVQVPSPANGVIEALLVPDGG 133
Query: 354 KV 359
KV
Sbjct: 134 KV 135
>sp|Q01205|ODO2_RAT Dihydrolipoyllysine-residue succinyltransferase component of
2-oxoglutarate dehydrogenase complex, mitochondrial
precursor (Dihydrolipoamide succinyltransferase
component of 2-oxoglutarate dehydrogenase complex) (E2)
(E2K)
Length = 454
Score = 79.3 bits (194), Expect = 4e-15
Identities = 40/85 (47%), Positives = 56/85 (65%)
Frame = +3
Query: 105 NRNKIYTIKREIGTSLFLFEDKIIKTPPFAESVTEGDVRWSKKPGDTVVVDEVLGEIETD 284
N + +++++ T++ + ++TP FAESVTEGDVRW K GD V DEV+ EIETD
Sbjct: 51 NNSSVFSVRFFQTTAVCKNDVITVQTPAFAESVTEGDVRWEKAVGDAVAEDEVVCEIETD 110
Query: 285 KTAIPIHSPFQGTILELIVSDGDKV 359
KT++ + SP G I L+V DG KV
Sbjct: 111 KTSVQVPSPANGIIEALLVPDGGKV 135
>sp|Q9D2G2|ODO2_MOUSE Dihydrolipoyllysine-residue succinyltransferase component of
2-oxoglutarate dehydrogenase complex, mitochondrial
precursor (Dihydrolipoamide succinyltransferase
component of 2-oxoglutarate dehydrogenase complex) (E2)
(E2K)
Length = 454
Score = 78.2 bits (191), Expect = 9e-15
Identities = 38/62 (61%), Positives = 45/62 (72%)
Frame = +3
Query: 174 IKTPPFAESVTEGDVRWSKKPGDTVVVDEVLGEIETDKTAIPIHSPFQGTILELIVSDGD 353
++TP FAESVTEGDVRW K GD V DEV+ EIETDKT++ + SP G I L+V DG
Sbjct: 74 VQTPAFAESVTEGDVRWEKAVGDAVAEDEVVCEIETDKTSVQVPSPANGIIEALLVPDGG 133
Query: 354 KV 359
KV
Sbjct: 134 KV 135
>sp|O94681|ODO2_SCHPO Probable dihydrolipoyllysine-residue succinyltransferase component
of 2-oxoglutarate dehydrogenase complex, mitochondrial
precursor (E2) (Probable dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex)
Length = 452
Score = 69.3 bits (168), Expect = 4e-12
Identities = 33/76 (43%), Positives = 48/76 (63%), Gaps = 1/76 (1%)
Frame = +3
Query: 174 IKTPPFAESVTEGDV-RWSKKPGDTVVVDEVLGEIETDKTAIPIHSPFQGTILELIVSDG 350
IKTPPF ES+TEG + +W K+PG+ V DE + +ETDK P+ +P G + E +V +G
Sbjct: 45 IKTPPFPESITEGTLAQWLKQPGEYVNKDEEIASVETDKIDAPVTAPDAGVLKEQLVKEG 104
Query: 351 DKVIANQDLVKISLGA 398
D + +QD+ I A
Sbjct: 105 DTITIDQDIAVIDTSA 120
>sp|P52993|ODO2_RALEU Dihydrolipoyllysine-residue succinyltransferase component of
2-oxoglutarate dehydrogenase complex (E2)
(Dihydrolipoamide succinyltransferase component of
2-oxoglutarate dehydrogenase complex)
Length = 416
Score = 65.1 bits (157), Expect = 7e-11
Identities = 31/76 (40%), Positives = 47/76 (61%), Gaps = 1/76 (1%)
Frame = +3
Query: 174 IKTPPFAESVTEGDV-RWSKKPGDTVVVDEVLGEIETDKTAIPIHSPFQGTILELIVSDG 350
+K P +ESV E + W KKPG+ V DE+L EIETDK + + +P G + ++ +DG
Sbjct: 6 VKVPQLSESVAEATMLNWKKKPGEAVAQDEILIEIETDKVVLEVPAPSAGVLSIIVKNDG 65
Query: 351 DKVIANQDLVKISLGA 398
D V+A++ + KI A
Sbjct: 66 DTVVADEIIAKIDTEA 81
Score = 30.4 bits (67), Expect = 2.0
Identities = 12/22 (54%), Positives = 17/22 (77%)
Frame = +3
Query: 228 KKPGDTVVVDEVLGEIETDKTA 293
K GDTVV DE++ +I+T+ TA
Sbjct: 62 KNDGDTVVADEIIAKIDTEATA 83
>sp|P19262|ODO2_YEAST Dihydrolipoyllysine-residue succinyltransferase component of
2-oxoglutarate dehydrogenase complex, mitochondrial
precursor (E2) (Dihydrolipoamide succinyltransferase
component of 2-oxoglutarate dehydrogenase complex)
Length = 463
Score = 62.0 bits (149), Expect = 6e-10
Identities = 36/100 (36%), Positives = 54/100 (54%), Gaps = 1/100 (1%)
Frame = +3
Query: 159 FEDKIIKTPPFAESVTEGDVR-WSKKPGDTVVVDEVLGEIETDKTAIPIHSPFQGTILEL 335
F+ I+ PP AES+TEG ++ ++K GD + DE+L IETDK I ++SP GT+ +L
Sbjct: 71 FKSTSIEVPPMAESLTEGSLKEYTKNVGDFIKEDELLATIETDKIDIEVNSPVSGTVTKL 130
Query: 336 IVSDGDKVIANQDLVKISLGAVTETKKDVVIETKIEKKPE 455
D V ++L ++ G + E E KPE
Sbjct: 131 NFKPEDTVTVGEELAQVEPG-------EAPAEGSGESKPE 163
>sp|P20708|ODO2_AZOVI Dihydrolipoyllysine-residue succinyltransferase component of
2-oxoglutarate dehydrogenase complex (E2)
(Dihydrolipoamide succinyltransferase component of
2-oxoglutarate dehydrogenase complex)
Length = 399
Score = 61.2 bits (147), Expect = 1e-09
Identities = 30/75 (40%), Positives = 48/75 (64%), Gaps = 1/75 (1%)
Frame = +3
Query: 174 IKTPPFAESVTEGDVR-WSKKPGDTVVVDEVLGEIETDKTAIPIHSPFQGTILELIVSDG 350
IK P F ES+ +G V W KKPG+ V DE++ +IETDK + + + G I E++ ++G
Sbjct: 5 IKAPTFPESIADGTVATWHKKPGEPVKRDELIVDIETDKVVMEVLAEADGVIAEIVKNEG 64
Query: 351 DKVIANQDLVKISLG 395
D V++ + L K++ G
Sbjct: 65 DTVLSGELLGKLTEG 79
>sp|P11961|ODP2_BACST Dihydrolipoyllysine-residue acetyltransferase component of pyruvate
dehydrogenase complex (E2) (Dihydrolipoamide
acetyltransferase component of pyruvate dehydrogenase
complex)
Length = 428
Score = 60.1 bits (144), Expect = 2e-09
Identities = 34/99 (34%), Positives = 51/99 (51%), Gaps = 3/99 (3%)
Frame = +3
Query: 177 KTPPFAESVTEGD-VRWSKKPGDTVVVDEVLGEIETDKTAIPIHSPFQGTILELIVSDGD 353
K P E + EG+ V+W KPGD V D+VL E++ DK + I SP +G +LE++V +G
Sbjct: 6 KLPDIGEGIHEGEIVKWFVKPGDEVNEDDVLCEVQNDKAVVEIPSPVKGKVLEILVPEGT 65
Query: 354 KVIANQDLVKISLGAVTET--KKDVVIETKIEKKPEVIT 464
Q L+ + K E K E+K E ++
Sbjct: 66 VATVGQTLITLDAPGYENMTFKGQEQEEAKKEEKTETVS 104
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 41,088,523
Number of Sequences: 369166
Number of extensions: 745225
Number of successful extensions: 2496
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2438
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2485
length of database: 68,354,980
effective HSP length: 101
effective length of database: 49,696,745
effective search space used: 2633927485
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)