Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_023_B07
(854 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8CFA2|GCST_MOUSE Aminomethyltransferase, mitochondrial ... 252 8e-67
sp|Q9TSZ7|GCST_CANFA Aminomethyltransferase, mitochondrial ... 248 2e-65
sp|P25285|GCST_BOVIN Aminomethyltransferase, mitochondrial ... 247 3e-65
sp|P48728|GCST_HUMAN Aminomethyltransferase, mitochondrial ... 246 8e-65
sp|P28337|GCST_CHICK Aminomethyltransferase, mitochondrial ... 232 1e-60
sp|O14110|GCST_SCHPO Probable aminomethyltransferase, mitoc... 213 5e-55
sp|P54260|GCST_SOLTU Aminomethyltransferase, mitochondrial ... 203 6e-52
sp|O49849|GCST_FLAAN Aminomethyltransferase, mitochondrial ... 201 3e-51
sp|O65396|GCST_ARATH Aminomethyltransferase, mitochondrial ... 200 5e-51
sp|P93256|GCST_MESCR Aminomethyltransferase, mitochondrial ... 200 5e-51
>sp|Q8CFA2|GCST_MOUSE Aminomethyltransferase, mitochondrial precursor (Glycine cleavage
system T protein) (GCVT)
Length = 403
Score = 252 bits (644), Expect = 8e-67
Identities = 134/261 (51%), Positives = 174/261 (66%), Gaps = 2/261 (0%)
Frame = +1
Query: 76 ARYISSIKP--EKTCLYDFHVRHHARMTEFAGYSMPLQYKSHSIIDSHKWVREKCGLFDV 249
+R +SS++ +T LYDFH+ H +M FAG+S+P+QY+ S +DSH R C LFDV
Sbjct: 23 SRPLSSVQDVLRRTPLYDFHLAHGGKMVAFAGWSLPVQYRD-SHVDSHLHTRRHCSLFDV 81
Query: 250 SHMLQFKIHGQKREQFIHGLTVIDMSELEPDYGSLSLFTNPNGGIMDDVIINNCSQPFYY 429
SHMLQ KI G R + + + V D++EL P+ G+LSLFTN GGI+DD+I++N S+ Y
Sbjct: 82 SHMLQTKIFGCDRVKLLESVVVGDIAELRPNQGTLSLFTNEAGGILDDLIVSNTSEGHLY 141
Query: 430 AVSNAGCAAKIKNHILTWEEKYQKMGNDIRIEFVENGLIALQGPLMTKVLQPGLEISLAN 609
VSNAGC K + +++Q G D+ +E VEN L+ALQGP T+VLQ G+ +
Sbjct: 142 VVSNAGCRDKDLALMQDKVKEFQNRGLDVGLEVVENALLALQGPTATQVLQAGVTDDMKK 201
Query: 610 LYFGRGVYCSVFGIPNCRVTRCGYTGEDGVEISVSKESADELAEKLVSHNDVIPVGLGAR 789
L F VFG+ CRVTRCGYTGEDGVEISV A LA L+ + +V GL AR
Sbjct: 202 LPFMTSAVMEVFGVSGCRVTRCGYTGEDGVEISVPATGAVHLATTLLKNPEVKLAGLAAR 261
Query: 790 DTLRLEAGLCLYGNDIDENTT 852
D+LRLEAGLCLYGNDIDE+TT
Sbjct: 262 DSLRLEAGLCLYGNDIDEHTT 282
>sp|Q9TSZ7|GCST_CANFA Aminomethyltransferase, mitochondrial precursor (Glycine cleavage
system T protein) (GCVT)
Length = 403
Score = 248 bits (633), Expect = 2e-65
Identities = 131/249 (52%), Positives = 167/249 (67%)
Frame = +1
Query: 106 KTCLYDFHVRHHARMTEFAGYSMPLQYKSHSIIDSHKWVREKCGLFDVSHMLQFKIHGQK 285
+T LYDFH+ H +M FAG+S+P+QY+ S +DSH R C LFDVSHMLQ KI G
Sbjct: 35 RTPLYDFHLAHGGKMVAFAGWSLPVQYRD-SHVDSHLHTRRHCSLFDVSHMLQTKILGCD 93
Query: 286 REQFIHGLTVIDMSELEPDYGSLSLFTNPNGGIMDDVIINNCSQPFYYAVSNAGCAAKIK 465
R + + L V D++EL P+ G+LSLFTN GGI DD+I+ + S+ + Y VSNAGC K
Sbjct: 94 RVKLMESLVVGDIAELRPNQGTLSLFTNEAGGIEDDLIVTSTSEGYLYVVSNAGCWDKDL 153
Query: 466 NHILTWEEKYQKMGNDIRIEFVENGLIALQGPLMTKVLQPGLEISLANLYFGRGVYCSVF 645
+ + Q MG+D+ +E V+N L+ALQGP T+VLQ G+ L L F VF
Sbjct: 154 ALMQGKVRELQNMGSDVSLEVVDNALLALQGPTATQVLQAGVADDLRKLPFMTSAVMEVF 213
Query: 646 GIPNCRVTRCGYTGEDGVEISVSKESADELAEKLVSHNDVIPVGLGARDTLRLEAGLCLY 825
G+ CRVTRCGYTGEDGVEISV +A LA L+ + +V GL ARD+LRLEAGLCLY
Sbjct: 214 GVSGCRVTRCGYTGEDGVEISVPAAAAVRLAAALLENPEVKLAGLAARDSLRLEAGLCLY 273
Query: 826 GNDIDENTT 852
G+DIDE+TT
Sbjct: 274 GSDIDEHTT 282
>sp|P25285|GCST_BOVIN Aminomethyltransferase, mitochondrial precursor (Glycine cleavage
system T protein) (GCVT)
Length = 397
Score = 247 bits (630), Expect = 3e-65
Identities = 128/249 (51%), Positives = 166/249 (66%)
Frame = +1
Query: 106 KTCLYDFHVRHHARMTEFAGYSMPLQYKSHSIIDSHKWVREKCGLFDVSHMLQFKIHGQK 285
+T LYDFH+ H +M FAG+S+P+QY+ S ++SH R+ C LFDVSHMLQ KI G
Sbjct: 29 RTPLYDFHLAHGGKMVAFAGWSLPVQYRD-SHVNSHLHTRQHCSLFDVSHMLQTKIFGCD 87
Query: 286 REQFIHGLTVIDMSELEPDYGSLSLFTNPNGGIMDDVIINNCSQPFYYAVSNAGCAAKIK 465
R + + L V D++EL+P+ G+LSLFTN GGI+DD+I+ + S+ Y VSNAGC K
Sbjct: 88 RVKLMESLVVGDIAELKPNQGTLSLFTNEAGGILDDLIVTSASEGHLYVVSNAGCREKDL 147
Query: 466 NHILTWEEKYQKMGNDIRIEFVENGLIALQGPLMTKVLQPGLEISLANLYFGRGVYCSVF 645
+ + Q G+D+ +E ++N L+ALQGP +VLQ G+ L L F VF
Sbjct: 148 TLMQDKVRELQNKGSDVALEVMDNALLALQGPTAAQVLQAGVADDLRKLPFMTSAVMEVF 207
Query: 646 GIPNCRVTRCGYTGEDGVEISVSKESADELAEKLVSHNDVIPVGLGARDTLRLEAGLCLY 825
G+ CRVTRCGYTGEDGVEISV A LA L+ + +V GL ARD+LRLEAGLCLY
Sbjct: 208 GVSGCRVTRCGYTGEDGVEISVPAAEAVHLAAALLKNPEVKLAGLAARDSLRLEAGLCLY 267
Query: 826 GNDIDENTT 852
GNDIDE+TT
Sbjct: 268 GNDIDEHTT 276
>sp|P48728|GCST_HUMAN Aminomethyltransferase, mitochondrial precursor (Glycine cleavage
system T protein) (GCVT)
Length = 403
Score = 246 bits (627), Expect = 8e-65
Identities = 129/249 (51%), Positives = 163/249 (65%)
Frame = +1
Query: 106 KTCLYDFHVRHHARMTEFAGYSMPLQYKSHSIIDSHKWVREKCGLFDVSHMLQFKIHGQK 285
+T LYDFH+ H +M FAG+S+P+QY+ S DSH R+ C LFDVSHMLQ KI G
Sbjct: 35 RTPLYDFHLAHGGKMVAFAGWSLPVQYRD-SHTDSHLHTRQHCSLFDVSHMLQTKILGSD 93
Query: 286 REQFIHGLTVIDMSELEPDYGSLSLFTNPNGGIMDDVIINNCSQPFYYAVSNAGCAAKIK 465
R + + L V D++EL P+ G+LSLFTN GGI+DD+I+ N S+ Y VSNAGC K
Sbjct: 94 RVKLMESLVVGDIAELRPNQGTLSLFTNEAGGILDDLIVTNTSEGHLYVVSNAGCWEKDL 153
Query: 466 NHILTWEEKYQKMGNDIRIEFVENGLIALQGPLMTKVLQPGLEISLANLYFGRGVYCSVF 645
+ + Q G D+ +E ++N L+ALQGP +VLQ G+ L L F VF
Sbjct: 154 ALMQDKVRELQNQGRDVGLEVLDNALLALQGPTAAQVLQAGVADDLRKLPFMTSAVMEVF 213
Query: 646 GIPNCRVTRCGYTGEDGVEISVSKESADELAEKLVSHNDVIPVGLGARDTLRLEAGLCLY 825
G+ CRVTRCGYTGEDGVEISV A LA ++ + +V GL ARD+LRLEAGLCLY
Sbjct: 214 GVSGCRVTRCGYTGEDGVEISVPVAGAVHLATAILKNPEVKLAGLAARDSLRLEAGLCLY 273
Query: 826 GNDIDENTT 852
GNDIDE+TT
Sbjct: 274 GNDIDEHTT 282
>sp|P28337|GCST_CHICK Aminomethyltransferase, mitochondrial precursor (Glycine cleavage
system T protein) (GCVT)
Length = 392
Score = 232 bits (591), Expect = 1e-60
Identities = 126/263 (47%), Positives = 165/263 (62%), Gaps = 2/263 (0%)
Frame = +1
Query: 70 LIARYISSIKP--EKTCLYDFHVRHHARMTEFAGYSMPLQYKSHSIIDSHKWVREKCGLF 243
L R++SS ++T L H RM FAG+S+P+QY ++SH R C LF
Sbjct: 10 LARRHLSSAPEGLKQTPLDALHRARGGRMVPFAGWSLPVQY-GRGHLESHLHTRRHCSLF 68
Query: 244 DVSHMLQFKIHGQKREQFIHGLTVIDMSELEPDYGSLSLFTNPNGGIMDDVIINNCSQPF 423
DVSHMLQ +++G+ R +F+ L V D++EL P G+L+L TN G I+DD+I+ N ++
Sbjct: 69 DVSHMLQTRVYGRDRVRFLESLVVGDIAELRPGQGTLTLLTNERGDIVDDLIVTNTAEDH 128
Query: 424 YYAVSNAGCAAKIKNHILTWEEKYQKMGNDIRIEFVENGLIALQGPLMTKVLQPGLEISL 603
Y VSNAGCA K + + + + G D+ +E +QGP M +VLQ GL L
Sbjct: 129 LYVVSNAGCADKDRAVMEGRAAELRAAGGDVHLEVSGQRAAGVQGPSMAQVLQAGLPDDL 188
Query: 604 ANLYFGRGVYCSVFGIPNCRVTRCGYTGEDGVEISVSKESADELAEKLVSHNDVIPVGLG 783
L F +VFG+P CRVTRCGYTGEDGVEISV A ELAE+L+ +V P GL
Sbjct: 189 TKLTFMTSTATTVFGVPGCRVTRCGYTGEDGVEISVPAGRAVELAERLLGCPEVWPAGLA 248
Query: 784 ARDTLRLEAGLCLYGNDIDENTT 852
ARD+LRLEAGLCLYGNDIDE+TT
Sbjct: 249 ARDSLRLEAGLCLYGNDIDESTT 271
>sp|O14110|GCST_SCHPO Probable aminomethyltransferase, mitochondrial precursor (Glycine
cleavage system T protein) (GCVT)
Length = 387
Score = 213 bits (542), Expect = 5e-55
Identities = 116/263 (44%), Positives = 165/263 (62%), Gaps = 8/263 (3%)
Frame = +1
Query: 88 SSIKPEKTCLYDFHVRHHARMTEFAGYSMPLQYKSHSIIDSHKWVREKCGLFDVSHMLQF 267
SS+K +T LYD H++ A + FAG+SMP+QYK +I SHKW RE GLFDVSHM+Q+
Sbjct: 20 SSLK--RTPLYDLHLKEGATIVPFAGFSMPVQYKGQTISASHKWTREHSGLFDVSHMVQW 77
Query: 268 KIHGQKREQFIHGLTVIDMSELEPDYGSLSLFTNPNGGIMDDVIINNCSQPFYYAVSNAG 447
+ G+ ++ +T + EL+P + +LS FTN GGI+DD II+ + YY V+NA
Sbjct: 78 FVRGENATAYLESITPSSLKELKPFHSTLSAFTNETGGIIDDTIISKQDENTYYIVTNAA 137
Query: 448 CA----AKIKNHILTWEEKYQKMGNDIRIEFVE-NGLIALQGPLMTKVLQ---PGLEISL 603
C+ A +K HI W+ + +E V+ LIA+QGP V+Q P ++ S+
Sbjct: 138 CSEKDEANLKKHIENWK--------GVELERVQGRALIAIQGPETASVVQKLIPNVDFSV 189
Query: 604 ANLYFGRGVYCSVFGIPNCRVTRCGYTGEDGVEISVSKESADELAEKLVSHNDVIPVGLG 783
L FG+ Y G+ C +R GYTGEDG E+S+ +E + + A L++ V P+GLG
Sbjct: 190 --LKFGQSAYVDFKGV-KCLFSRSGYTGEDGFEVSIPEEVSVDFASTLLADTRVRPIGLG 246
Query: 784 ARDTLRLEAGLCLYGNDIDENTT 852
ARDTLRLEAG+CLYG+DID+ T+
Sbjct: 247 ARDTLRLEAGMCLYGSDIDDTTS 269
>sp|P54260|GCST_SOLTU Aminomethyltransferase, mitochondrial precursor (Glycine cleavage
system T protein) (GCVT)
Length = 406
Score = 203 bits (516), Expect = 6e-52
Identities = 121/271 (44%), Positives = 160/271 (59%), Gaps = 5/271 (1%)
Frame = +1
Query: 55 QISLKLIAR--YISSIKPEKTCLYDFHVRHHARMTEFAGYSMPLQYKSHSIIDSHKWVRE 228
Q K I R + S +KT LYDFHV + +M FAG+SMP+QYK SI+DS RE
Sbjct: 18 QADKKTIGRRCFASDADLKKTVLYDFHVVNGGKMVPFAGWSMPIQYKD-SIMDSTVNCRE 76
Query: 229 KCGLFDVSHMLQFKIHGQKREQFIHGLTVIDMSELEPDYGSLSLFTNPNGGIMDDVIINN 408
LFDVSHM + G+ F+ L + D++ L P GSL++FTN GG +DD ++
Sbjct: 77 NGSLFDVSHMCGLSLKGKDTIPFLEKLVIADVAGLAPGTGSLTVFTNEKGGAIDDSVVTK 136
Query: 409 CSQPFYYAVSNAGCAAKIKNHILTWEEKYQKMGNDIRIEF-VENGLIALQGPLMTKVLQP 585
+ Y V NAGC K HI + ++ G D+ E L+ALQGPL VLQ
Sbjct: 137 VTNDHIYLVVNAGCRDKDLAHIEEHMKSFKSKGGDVSWHIHDERSLLALQGPLAAPVLQY 196
Query: 586 GLEISLANLYFGRGVYCSVFGIPNCRVTRCGYTGEDGVEISVSKESADELAEKLV--SHN 759
+ L+ +YFG + G P C +TR GYTGEDG EISV E+A +LA+ L+ S
Sbjct: 197 LTKDDLSKMYFGEFRVLDINGAP-CFLTRTGYTGEDGFEISVPSENALDLAKALLEKSEG 255
Query: 760 DVIPVGLGARDTLRLEAGLCLYGNDIDENTT 852
+ GLGARD+LRLEAGLCLYGND++++TT
Sbjct: 256 KIRLTGLGARDSLRLEAGLCLYGNDMEQHTT 286
>sp|O49849|GCST_FLAAN Aminomethyltransferase, mitochondrial precursor (Glycine cleavage
system T protein) (GCVT)
Length = 407
Score = 201 bits (510), Expect = 3e-51
Identities = 122/271 (45%), Positives = 158/271 (58%), Gaps = 5/271 (1%)
Frame = +1
Query: 55 QISLKLIAR--YISSIKPEKTCLYDFHVRHHARMTEFAGYSMPLQYKSHSIIDSHKWVRE 228
Q K I R Y S +KT LYDFHV H +M FAG+SMP+QYK SI+DS RE
Sbjct: 18 QSDKKTIVRRCYASEADLKKTVLYDFHVAHGGKMVPFAGWSMPIQYKD-SIMDSTINCRE 76
Query: 229 KCGLFDVSHMLQFKIHGQKREQFIHGLTVIDMSELEPDYGSLSLFTNPNGGIMDDVIINN 408
LFDVSHM + G+ F+ L V D++ L P GSL++FTN GG +DD +I
Sbjct: 77 NGSLFDVSHMCGLSLKGKDCVAFLEKLVVADVAGLAPGTGSLTVFTNEKGGAIDDSVITK 136
Query: 409 CSQPFYYAVSNAGCAAKIKNHILTWEEKYQKMGNDIRIE-FVENGLIALQGPLMTKVLQP 585
+ Y V NAGC K HI + ++ G D+ + E L+ALQGPL LQ
Sbjct: 137 VTDDHIYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIYDERSLLALQGPLAGSTLQH 196
Query: 586 GLEISLANLYFGRGVYCSVFGIPNCRVTRCGYTGEDGVEISVSKESADELAEKLV--SHN 759
+ L+ +YFG + G C +TR GYTGEDG EISV E+A +LA+ ++ S
Sbjct: 197 LTKEDLSKMYFGDFRIIDING-SKCFLTRTGYTGEDGFEISVPSENAVDLAKAILEKSEG 255
Query: 760 DVIPVGLGARDTLRLEAGLCLYGNDIDENTT 852
V GLGARD+LRLEAGLCLYGND++++ T
Sbjct: 256 KVRLTGLGARDSLRLEAGLCLYGNDMEQHIT 286
>sp|O65396|GCST_ARATH Aminomethyltransferase, mitochondrial precursor (Glycine cleavage
system T protein) (GCVT)
Length = 408
Score = 200 bits (508), Expect = 5e-51
Identities = 120/269 (44%), Positives = 158/269 (58%), Gaps = 5/269 (1%)
Frame = +1
Query: 55 QISLKLIAR--YISSIKPEKTCLYDFHVRHHARMTEFAGYSMPLQYKSHSIIDSHKWVRE 228
Q K+++R + S +KT LYDFHV H +M FAG+SMP+QYK SI+DS RE
Sbjct: 19 QSDKKVVSRRYFASEADLKKTALYDFHVAHGGKMVPFAGWSMPIQYKD-SIMDSTVNCRE 77
Query: 229 KCGLFDVSHMLQFKIHGQKREQFIHGLTVIDMSELEPDYGSLSLFTNPNGGIMDDVIINN 408
LFDV+HM + G+ F+ L V D++ L P GSL++FTN GG +DD +I
Sbjct: 78 NGSLFDVAHMCGLSLKGKDCVPFLETLVVADVAGLAPGTGSLTVFTNEKGGAIDDSVITK 137
Query: 409 CSQPFYYAVSNAGCAAKIKNHILTWEEKYQKMGNDIRIEF-VENGLIALQGPLMTKVLQP 585
+ Y V NAGC K HI + ++ G D+ E L+ALQGPL VLQ
Sbjct: 138 VTDEHIYLVVNAGCRDKDLAHIEEHMKAFKSKGGDVSWHIHDERSLLALQGPLAAPVLQH 197
Query: 586 GLEISLANLYFGRGVYCSVFGIPNCRVTRCGYTGEDGVEISVSKESADELAEKLV--SHN 759
+ L+ LYFG + G C +TR GYTGEDG EISV E A +LA+ ++ S
Sbjct: 198 LTKEDLSKLYFGNFQILDING-STCFLTRTGYTGEDGFEISVPDEHAVDLAKAILEKSEG 256
Query: 760 DVIPVGLGARDTLRLEAGLCLYGNDIDEN 846
V GLGARD+LRLEAGLCLYGND++++
Sbjct: 257 KVRLTGLGARDSLRLEAGLCLYGNDMEQH 285
>sp|P93256|GCST_MESCR Aminomethyltransferase, mitochondrial precursor (Glycine cleavage
system T protein) (GCVT)
Length = 408
Score = 200 bits (508), Expect = 5e-51
Identities = 120/271 (44%), Positives = 159/271 (58%), Gaps = 5/271 (1%)
Frame = +1
Query: 55 QISLKLIAR--YISSIKPEKTCLYDFHVRHHARMTEFAGYSMPLQYKSHSIIDSHKWVRE 228
Q K+IAR + S +KT LYDFHV + +M FAG+SMP+QYK SI+DS RE
Sbjct: 19 QAEKKVIARRCFASEADLKKTALYDFHVANGGKMVPFAGWSMPIQYKD-SIMDSTINCRE 77
Query: 229 KCGLFDVSHMLQFKIHGQKREQFIHGLTVIDMSELEPDYGSLSLFTNPNGGIMDDVIINN 408
LFDV+HM + G+ F+ L V D++ L P G+LS+ TN GG +DD +I
Sbjct: 78 NGSLFDVAHMCGLSLKGKDCIPFLEKLVVGDIAGLAPGTGTLSVLTNEKGGAIDDTVITK 137
Query: 409 CSQPFYYAVSNAGCAAKIKNHILTWEEKYQKMGNDIRIEF-VENGLIALQGPLMTKVLQP 585
+ Y V NAGC K HI + ++ G D+ E L+ALQGPL VLQ
Sbjct: 138 VTDDHIYLVVNAGCREKDLAHIEEHMKAFKAKGGDVSWHIHDERSLLALQGPLAAPVLQH 197
Query: 586 GLEISLANLYFGRGVYCSVFGIPNCRVTRCGYTGEDGVEISVSKESADELAEKLV--SHN 759
+ L+ YFG+ + + G P C +TR GYTGEDG EISV E A +LA+ ++ S
Sbjct: 198 LTKEDLSKFYFGQFTFLDINGFP-CYLTRTGYTGEDGFEISVPNEYAVDLAKAMLEKSEG 256
Query: 760 DVIPVGLGARDTLRLEAGLCLYGNDIDENTT 852
V G GARD+LRLEAGLCLYGND++++ T
Sbjct: 257 KVRLTGRGARDSLRLEAGLCLYGNDLEQHIT 287
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 95,936,579
Number of Sequences: 369166
Number of extensions: 2023011
Number of successful extensions: 5775
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5276
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5445
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8438301375
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)