Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_023_A14
(895 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q19425|YSPK_CAEEL Hypothetical protein F13H10.3 in chrom... 140 4e-33
sp|P0AAD4|TYRP_ECOLI Tyrosine-specific transport protein (T... 47 9e-05
sp|Q99624|S38A3_HUMAN System N amino acid transporter 1 (SN... 44 5e-04
sp|Q9DCP2|S38A3_MOUSE System N amino acid transporter 1 (SN... 44 6e-04
sp|Q9JHZ9|S38A3_RAT System N amino acid transporter 1 (SN1)... 44 6e-04
sp|P31122|SOTB_ECOLI Sugar efflux transporter 36 0.17
sp|Q7UCG7|SOTB_SHIFL Probable sugar efflux transporter 36 0.17
sp|P58529|SOTB_ECO57 Probable sugar efflux transporter 36 0.17
sp|Q8FHE6|SOTB_ECOL6 Probable sugar efflux transporter 36 0.17
sp|Q5L5E1|G6PI_CHLAB Glucose-6-phosphate isomerase (GPI) (P... 36 0.17
>sp|Q19425|YSPK_CAEEL Hypothetical protein F13H10.3 in chromosome IV
Length = 615
Score = 140 bits (354), Expect = 4e-33
Identities = 74/156 (47%), Positives = 101/156 (64%), Gaps = 9/156 (5%)
Frame = +1
Query: 136 RYRYYLRLGVP----VIPDHVLPSRFFVLLPSQTTPP--GTQSSFITIFSIWNTMMGTSL 297
RYR Y RL +PDHVLP F +LP + G Q S +TIFSIWNTMMGTSL
Sbjct: 122 RYRLYNRLDPGGEHLTMPDHVLPPNLFSILPFEELKDVSGKQGSIVTIFSIWNTMMGTSL 181
Query: 298 LSMPWAIQQSGFALGIFLMILVAGLAFYTAYLVLKS---TENLPLEPGRTAFTSDFSDAC 468
L+MPWA+QQ+G LGI +M+ +A + FYTAY+V++S ++L ++P ++FSD C
Sbjct: 182 LAMPWALQQAGLVLGIIIMLSMAAICFYTAYIVIESPKRLQDLSVDP----LLAEFSDVC 237
Query: 469 KFYLGRPGEIVSIIFSFLALLSGLAVYYVLLCNVLY 576
K GR GE +++FS L+ G+ VY+VL+ N LY
Sbjct: 238 KSLFGRIGEYCAVVFSVCVLIGGVIVYWVLMSNFLY 273
Score = 30.4 bits (67), Expect = 7.0
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = +2
Query: 815 FDKLWNQTHTVPFWLLIVVLPLISIKS 895
FDK W TVP +L + PL++ KS
Sbjct: 339 FDKFWTLRGTVPIYLAFALFPLMNFKS 365
>sp|P0AAD4|TYRP_ECOLI Tyrosine-specific transport protein (Tyrosine permease)
sp|P0AAD5|TYRP_SHIFL Tyrosine-specific transport protein (Tyrosine permease)
Length = 403
Score = 46.6 bits (109), Expect = 9e-05
Identities = 30/100 (30%), Positives = 53/100 (53%)
Frame = +1
Query: 256 TIFSIWNTMMGTSLLSMPWAIQQSGFALGIFLMILVAGLAFYTAYLVLKSTENLPLEPGR 435
++F + T +G +L+MP A GF++ + L+I + L YTA L+L+ +++P + G
Sbjct: 8 SVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLWALMCYTALLLLEVYQHVPADTG- 66
Query: 436 TAFTSDFSDACKFYLGRPGEIVSIIFSFLALLSGLAVYYV 555
K YLGR G+ ++ FS + L+ L Y+
Sbjct: 67 ------LGTLAKRYLGRYGQWLT-GFSMMFLMYALTAAYI 99
>sp|Q99624|S38A3_HUMAN System N amino acid transporter 1 (SN1) (N-system amino acid
transporter 1) (Solute carrier family 38 member 3)
Length = 504
Score = 44.3 bits (103), Expect = 5e-04
Identities = 38/155 (24%), Positives = 74/155 (47%), Gaps = 18/155 (11%)
Frame = +1
Query: 214 PSQTTPPGTQSSFITIFSIWNTMMGTSLLSMPWAIQQSGFALGIFLMILVAGLAFYTAYL 393
P T G S +++F++ N +MG+ +L + +A+ +G L +FL+ VA L+ Y+ +L
Sbjct: 57 PHFTDFEGKTSFGMSVFNLSNAIMGSGILGLAYAMANTGIILFLFLLTAVALLSSYSIHL 116
Query: 394 VLKSTENLPL----EPGRTAFTSDFSDACKFYLGRPGEIVSIIFSFLALLSGLAVYYVLL 561
+LKS+ + + + G AF G PG++ + + L + ++ Y ++
Sbjct: 117 LLKSSGVVGIRAYEQLGYRAF------------GTPGKLAAALAITLQNIGAMSSYLYII 164
Query: 562 CNVLYCAVN------------YLNG--LISLNSIT 624
+ L + Y+NG L+ L S+T
Sbjct: 165 KSELPLVIQTFLNLEEKTSDWYMNGNYLVILVSVT 199
>sp|Q9DCP2|S38A3_MOUSE System N amino acid transporter 1 (SN1) (N-system amino acid
transporter 1) (Solute carrier family 38 member 3)
(mNAT)
Length = 505
Score = 43.9 bits (102), Expect = 6e-04
Identities = 22/65 (33%), Positives = 41/65 (63%)
Frame = +1
Query: 214 PSQTTPPGTQSSFITIFSIWNTMMGTSLLSMPWAIQQSGFALGIFLMILVAGLAFYTAYL 393
P T G S +++F++ N +MG+ +L + +A+ +G L +FL+ VA L+ Y+ +L
Sbjct: 56 PHFTDFEGKTSFGMSVFNLSNAIMGSGILGLAYAMANTGIILFLFLLTAVALLSSYSIHL 115
Query: 394 VLKST 408
+LKS+
Sbjct: 116 LLKSS 120
>sp|Q9JHZ9|S38A3_RAT System N amino acid transporter 1 (SN1) (N-system amino acid
transporter 1) (Solute carrier family 38 member 3)
Length = 504
Score = 43.9 bits (102), Expect = 6e-04
Identities = 22/65 (33%), Positives = 41/65 (63%)
Frame = +1
Query: 214 PSQTTPPGTQSSFITIFSIWNTMMGTSLLSMPWAIQQSGFALGIFLMILVAGLAFYTAYL 393
P T G S +++F++ N +MG+ +L + +A+ +G L +FL+ VA L+ Y+ +L
Sbjct: 56 PHFTDFEGKTSFGMSVFNLSNAIMGSGILGLAYAMANTGIILFLFLLTAVALLSSYSIHL 115
Query: 394 VLKST 408
+LKS+
Sbjct: 116 LLKSS 120
>sp|P31122|SOTB_ECOLI Sugar efflux transporter
Length = 396
Score = 35.8 bits (81), Expect = 0.17
Identities = 28/99 (28%), Positives = 47/99 (47%), Gaps = 5/99 (5%)
Frame = +1
Query: 292 SLLSMPWAIQQSGFALGIFLMILVAGLAFYTAYLVLKSTENLPLEPGRTAFTSDFSDACK 471
SL S+P ++ + I+L+ +V A YTAY ++ P F+++F+ A
Sbjct: 198 SLKSLPLLFRRPAL-MSIYLLTVVVVTAHYTAYSYIE-----PFVQNIAGFSANFATALL 251
Query: 472 FYLGRPGEIVSIIF-----SFLALLSGLAVYYVLLCNVL 573
LG G I S+IF + + L A+ +L+C L
Sbjct: 252 LLLGGAGIIGSVIFGKLGNQYASALVSTAIALLLVCLAL 290
>sp|Q7UCG7|SOTB_SHIFL Probable sugar efflux transporter
Length = 396
Score = 35.8 bits (81), Expect = 0.17
Identities = 28/99 (28%), Positives = 47/99 (47%), Gaps = 5/99 (5%)
Frame = +1
Query: 292 SLLSMPWAIQQSGFALGIFLMILVAGLAFYTAYLVLKSTENLPLEPGRTAFTSDFSDACK 471
SL S+P ++ + I+L+ +V A YTAY ++ P F+++F+ A
Sbjct: 198 SLKSLPLLFRRPAL-MSIYLLTVVVVTAHYTAYSYIE-----PFVQNIAGFSANFATALL 251
Query: 472 FYLGRPGEIVSIIF-----SFLALLSGLAVYYVLLCNVL 573
LG G I S+IF + + L A+ +L+C L
Sbjct: 252 LLLGGAGIIGSVIFGKLGNQYASALVSTAIALLLVCLAL 290
>sp|P58529|SOTB_ECO57 Probable sugar efflux transporter
Length = 396
Score = 35.8 bits (81), Expect = 0.17
Identities = 28/99 (28%), Positives = 47/99 (47%), Gaps = 5/99 (5%)
Frame = +1
Query: 292 SLLSMPWAIQQSGFALGIFLMILVAGLAFYTAYLVLKSTENLPLEPGRTAFTSDFSDACK 471
SL S+P ++ + I+L+ +V A YTAY ++ P F+++F+ A
Sbjct: 198 SLKSLPLLFRRPAL-MSIYLLTVVVVTAHYTAYSYIE-----PFVQNIAGFSANFATALL 251
Query: 472 FYLGRPGEIVSIIF-----SFLALLSGLAVYYVLLCNVL 573
LG G I S+IF + + L A+ +L+C L
Sbjct: 252 LLLGGAGIIGSVIFGKLGNQYASALVSTAIALLLVCLAL 290
>sp|Q8FHE6|SOTB_ECOL6 Probable sugar efflux transporter
Length = 396
Score = 35.8 bits (81), Expect = 0.17
Identities = 28/99 (28%), Positives = 47/99 (47%), Gaps = 5/99 (5%)
Frame = +1
Query: 292 SLLSMPWAIQQSGFALGIFLMILVAGLAFYTAYLVLKSTENLPLEPGRTAFTSDFSDACK 471
SL S+P ++ + I+L+ +V A YTAY ++ P F+++F+ A
Sbjct: 198 SLKSLPLLFRRPAL-MSIYLLTVVVVTAHYTAYSYIE-----PFVQNIAGFSANFATALL 251
Query: 472 FYLGRPGEIVSIIF-----SFLALLSGLAVYYVLLCNVL 573
LG G I S+IF + + L A+ +L+C L
Sbjct: 252 LLLGGAGIIGSVIFGKLGNQYASALVSTAIALLLVCLAL 290
>sp|Q5L5E1|G6PI_CHLAB Glucose-6-phosphate isomerase (GPI) (Phosphoglucose isomerase)
(PGI) (Phosphohexose isomerase) (PHI)
Length = 530
Score = 35.8 bits (81), Expect = 0.17
Identities = 36/135 (26%), Positives = 61/135 (45%), Gaps = 14/135 (10%)
Frame = +1
Query: 61 YSAYSANPDNVSDSEISVNWSASL----SRYRYYLRLGV--PVIPDHVLPSRF-----FV 207
Y + +PDN ++ ++ S +L S+ L V I DH L F+
Sbjct: 174 YFVSNIDPDNAAEVLQEIDCSKTLVVTVSKSGTTLETAVNEEFIADHFLKQGLHFRDHFI 233
Query: 208 LLPSQTTPPGTQSSFITIFSIWNTMMGT-SLLSMPWAIQQSGFALG--IFLMILVAGLAF 378
+ + +P +S ++ +F IW+++ G S SM + GFA G +F +L A
Sbjct: 234 AVTCEGSPMDDRSKYLEVFHIWDSIGGRYSSTSMVGGVVL-GFAYGFDVFFQLLEGAAAM 292
Query: 379 YTAYLVLKSTENLPL 423
A L + +ENLP+
Sbjct: 293 DLAALAPQMSENLPM 307
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 98,441,125
Number of Sequences: 369166
Number of extensions: 1948983
Number of successful extensions: 4882
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4726
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4879
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8982382310
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)