Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_022_P23
(539 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q57862|Y419_METJA Hypothetical protein MJ0419 34 0.20
sp|Q9CG19|GADC_LACLA Glutamate/gamma-aminobutyrate antiporter 33 0.34
sp|Q9M3J4|NU5C_SPIOL NAD(P)H-quinone oxidoreductase chain 5... 32 0.75
sp|Q8D1U8|KUP_WIGBR Low affinity potassium transport system... 32 0.98
sp|P55013|S12A2_SQUAC Solute carrier family 12 member 2 (Bu... 31 1.7
sp|Q99N05|M4A4D_MOUSE Membrane-spanning 4-domains subfamily... 31 2.2
sp|Q58389|Y979_METJA Hypothetical protein MJ0979 31 2.2
sp|P53109|YGR0_YEAST Hypothetical 65.8 kDa protein in SUT1-... 30 2.9
sp|P08006|OPPC_SALTY Oligopeptide transport system permease... 30 3.7
sp|Q9D7W4|TSN17_MOUSE Tetraspanin-17 (Tspan-17) (Transmembr... 30 3.7
>sp|Q57862|Y419_METJA Hypothetical protein MJ0419
Length = 354
Score = 34.3 bits (77), Expect = 0.20
Identities = 29/121 (23%), Positives = 56/121 (46%), Gaps = 10/121 (8%)
Frame = +2
Query: 32 KHKGFGITFTVIGALAIILGIVTV---------SSNASNIWFILVNGIVLTIAGSMSIYS 184
KH FGI ++G +A+ ++ V S N++F ++ + L + ++ + S
Sbjct: 73 KHYNFGIFLMIVGLIAVTSDLIWVKDVPLFNPLSRKFLNVYFTTLSHLFL-VGWAIVVAS 131
Query: 185 SSHPTSRNLVITLIFEIISCVVYILSF-IGSIVLIIFVSIAAKDDNVDKKAVVIIYGFLG 361
S+ + L+ T+IF I+ + +L + +VL+I V N ++ YG L
Sbjct: 132 SNIDKKKILLYTIIFSIL---IMLLGYRTNVLVLLISVGAILYYKNKISNREILKYGILV 188
Query: 362 F 364
F
Sbjct: 189 F 189
>sp|Q9CG19|GADC_LACLA Glutamate/gamma-aminobutyrate antiporter
Length = 503
Score = 33.5 bits (75), Expect = 0.34
Identities = 29/105 (27%), Positives = 48/105 (45%), Gaps = 2/105 (1%)
Frame = +2
Query: 131 ILVNGIVLTIAGSMSIYSSSHPTSRNLVITLIFEIISCVVYILSFIGSIVLIIFVSIAAK 310
+++ GI++T+ G++ + LV + +I V Y+L FIG VLI +
Sbjct: 335 VMIQGIIVTLWGAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFIGYFVLIYKKQNLKR 394
Query: 311 DDNVDKKAV--VIIYGFLGFWLFCAGLMTILLIISVVYINKDYKY 439
NV K V II G +GF L L + + + N+ + Y
Sbjct: 395 TYNVPGKRVGKTIIAG-IGFLLSIFALFISFVPPASIAKNETHTY 438
>sp|Q9M3J4|NU5C_SPIOL NAD(P)H-quinone oxidoreductase chain 5, chloroplast (NAD(P)H
dehydrogenase, chain 5) (NADH-plastoquinone
oxidoreductase chain 5)
Length = 742
Score = 32.3 bits (72), Expect = 0.75
Identities = 12/54 (22%), Positives = 29/54 (53%)
Frame = +2
Query: 224 IFEIISCVVYILSFIGSIVLIIFVSIAAKDDNVDKKAVVIIYGFLGFWLFCAGL 385
+F +I ++Y++SFIG I +++ ++A ++ + LG+ + G+
Sbjct: 283 LFVVIPYIMYVISFIGIITVLLGATLALAQKDIKRSLAYYTMSQLGYMMLALGM 336
>sp|Q8D1U8|KUP_WIGBR Low affinity potassium transport system protein kup (Kup system
potassium uptake protein)
Length = 625
Score = 32.0 bits (71), Expect = 0.98
Identities = 38/146 (26%), Positives = 65/146 (44%), Gaps = 16/146 (10%)
Frame = +2
Query: 53 TFTVIGALAIILGIVTVSSNASNIWFILVNGIVLTIAGSMSIYSSSHPTSRNLVITLIFE 232
TFT AII+ I++ + S+I+++ + L ++ I+ +S SR + I I
Sbjct: 292 TFT----FAIIISIISSQNIISSIFYLTRQAVRLGYLPNIKIFYTSEVKSRRIYIPCINW 347
Query: 233 I--ISCVVYILSF-----------IGSIV---LIIFVSIAAKDDNVDKKAVVIIYGFLGF 364
I +S V+ I F IGSI+ L F S+ +K ++ I
Sbjct: 348 IFYLSAVIQISIFKNLHNLILIYGIGSIITMSLTTFFSLLFFKKKFEKFKILKI------ 401
Query: 365 WLFCAGLMTILLIISVVYINKDYKYI 442
L+TIL++ ++I+ YK I
Sbjct: 402 ----TFLLTILILEFFIFISNSYKII 423
>sp|P55013|S12A2_SQUAC Solute carrier family 12 member 2 (Bumetanide-sensitive
sodium-(potassium)-chloride cotransporter 1) (NA-K-CL
symporter) (NKCC)
Length = 1191
Score = 31.2 bits (69), Expect = 1.7
Identities = 31/135 (22%), Positives = 52/135 (38%), Gaps = 27/135 (20%)
Frame = +2
Query: 35 HKGFGITFTVIGALAIILGIVTVSSNASNIWFILVNGIVLTIAGSMSIYSSSHPTSRNLV 214
H G G+ VIG ++ I +S++A I NG V G+ + S S
Sbjct: 286 HAGIGLALLVIGTATVVTTITGLSTSA-----ITTNGFVRG-GGAYYLISRSLGPEFGGA 339
Query: 215 ITLIFEIISCV---VYILSF------------------------IGSIVLIIFVSIAAKD 313
I LIF + V +Y++ F IGS+ +++ I+
Sbjct: 340 IGLIFAFANAVAVAMYVVGFAETVRDLLVEHNALMIDEMSDIRIIGSVTIVVLFGISVAG 399
Query: 314 DNVDKKAVVIIYGFL 358
+ KA +++ G L
Sbjct: 400 MEWEAKAQIVLLGIL 414
>sp|Q99N05|M4A4D_MOUSE Membrane-spanning 4-domains subfamily A member 4D
Length = 225
Score = 30.8 bits (68), Expect = 2.2
Identities = 21/93 (22%), Positives = 43/93 (46%), Gaps = 6/93 (6%)
Frame = +2
Query: 29 GKHKGFGITFTVIGALAIILGIVTVSSNASNIWFILV------NGIVLTIAGSMSIYSSS 190
G+ K G +I + LGI+ + + S + ++ I+ +GS+SI +
Sbjct: 39 GEPKVLGAIQVMIAFINFSLGIIIILNRVSERFMSVLLLAPFWGSIMFIFSGSLSIAAGV 98
Query: 191 HPTSRNLVITLIFEIISCVVYILSFIGSIVLII 289
PT ++ +L IS V+ + + I ++ +I
Sbjct: 99 KPTKAMIISSLSVNTISSVLAVAASIIGVISVI 131
>sp|Q58389|Y979_METJA Hypothetical protein MJ0979
Length = 197
Score = 30.8 bits (68), Expect = 2.2
Identities = 31/127 (24%), Positives = 56/127 (44%), Gaps = 6/127 (4%)
Frame = +2
Query: 50 ITFTVIGALAIILGIVTVSSNASNIWFILVNGIVLTI-AGSMSIYSSSHPTSRNLVITLI 226
I V+G +A+ L +T+S++ NI GI L++ + S + N+ + ++
Sbjct: 11 IALIVVGIIALFLPWLTISASTINI--KTDEGIHLSVNLAPFRVSSDIKSDTNNIFVEMM 68
Query: 227 FEIIS-----CVVYILSFIGSIVLIIFVSIAAKDDNVDKKAVVIIYGFLGFWLFCAGLMT 391
+ V +S I II + + VDKKAVV+ G G + CA +
Sbjct: 69 MPYVKQYFDMAVKEKMSTFMMIFGIIPIILYIASIFVDKKAVVVGAGIAG--ITCASIFV 126
Query: 392 ILLIISV 412
+L + +
Sbjct: 127 VLFTVGL 133
>sp|P53109|YGR0_YEAST Hypothetical 65.8 kDa protein in SUT1-RCK1 intergenic region
Length = 570
Score = 30.4 bits (67), Expect = 2.9
Identities = 24/78 (30%), Positives = 37/78 (47%)
Frame = +2
Query: 254 ILSFIGSIVLIIFVSIAAKDDNVDKKAVVIIYGFLGFWLFCAGLMTILLIISVVYINKDY 433
I++ IG + I F+ A DDNV K +I+ F F F ++ + L+I + + Y
Sbjct: 142 IITVIGLLHGIFFIIKWAIDDNVSLKQKLILKTF-NFAGFIISILVLFLLICSIGPMRRY 200
Query: 434 KYIRQQ*FFQTVRNCVNI 487
Y F V N VN+
Sbjct: 201 NYR----LFYIVHNLVNV 214
>sp|P08006|OPPC_SALTY Oligopeptide transport system permease protein oppC
Length = 302
Score = 30.0 bits (66), Expect = 3.7
Identities = 25/81 (30%), Positives = 40/81 (49%), Gaps = 3/81 (3%)
Frame = +2
Query: 68 GALAIILGIVTVSSNASNIWFILVNGIVLTIAGSMSIYSSSHPTS---RNLVITLIFEII 238
G +++++GI LV IV T+ GS+S Y S R L I F +
Sbjct: 101 GRISLMVGIAAA----------LVAVIVGTLYGSLSGYLGGKIDSVMMRLLEILNSFPFM 150
Query: 239 SCVVYILSFIGSIVLIIFVSI 301
V+ +++F G +L+IFV+I
Sbjct: 151 FFVILLVTFFGQNILLIFVAI 171
>sp|Q9D7W4|TSN17_MOUSE Tetraspanin-17 (Tspan-17) (Transmembrane 4 superfamily member 17)
(Tetraspan protein SB134) (F-box only protein 23)
Length = 270
Score = 30.0 bits (66), Expect = 3.7
Identities = 23/98 (23%), Positives = 40/98 (40%), Gaps = 14/98 (14%)
Frame = +2
Query: 41 GFGITFTVIGALAIILGIVT-----VSSNAS---------NIWFILVNGIVLTIAGSMSI 178
GF I F V+GAL + +G+ V SN S +W +V G V+++ G
Sbjct: 23 GFNIVFWVLGALFLAIGLWAWGEKGVLSNISALTDLGGLDPVWLFVVVGGVMSVLGFAGC 82
Query: 179 YSSSHPTSRNLVITLIFEIISCVVYILSFIGSIVLIIF 292
+ N + F + +++ L I+ +F
Sbjct: 83 IGA---LRENTFLLKFFSVFLGLIFFLELAAGILAFVF 117
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57,263,904
Number of Sequences: 369166
Number of extensions: 1088766
Number of successful extensions: 3320
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3315
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 3685690500
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)