Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_022_P17
(723 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 129 6e-30
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 129 8e-30
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 124 2e-28
sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinas... 113 4e-25
sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinas... 112 8e-25
sp|P27636|CDC15_YEAST Cell division control protein 15 112 8e-25
sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinas... 112 1e-24
sp|Q13153|PAK1_HUMAN Serine/threonine-protein kinase PAK 1 ... 112 1e-24
sp|Q01389|BCK1_YEAST Serine/threonine-protein kinase BCK1/S... 111 2e-24
sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (S... 110 3e-24
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 129 bits (325), Expect = 6e-30
Identities = 85/242 (35%), Positives = 129/242 (53%), Gaps = 1/242 (0%)
Frame = +1
Query: 1 HPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHS 180
HP I++ G R + I L+++ GS+ S L K GA E + ++ Q+L L++LH+
Sbjct: 130 HPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRTYTNQLLLGLEYLHN 189
Query: 181 QNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVI 360
I+HRD+KG IL+DN IKL F ++K + ++ + A+S GT W APEVI
Sbjct: 190 HAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATIS----GAKSMKGTPYWMAPEVI 245
Query: 361 NGEGYGSSADIWSFGAVMVELITGKAPLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALK 537
G+ SADIWS G ++E++TGKAP AI+ +GT K P S
Sbjct: 246 LQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKS-HPPIPDNISSDAN 304
Query: 538 SFVDPCLDPEPLNRPTVKDLMKHEFLNSAMEAD*FT*RVDIFSWIN*NNTYMLPK*INNC 717
F+ CL EP RPT +L+KH F+ + + D+ S+++ N+ LP + N
Sbjct: 305 DFLLKCLQQEPNLRPTASELLKHPFVTGKQKE---SASKDLTSFMD-NSCSPLPSELTNI 360
Query: 718 SS 723
+S
Sbjct: 361 TS 362
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 129 bits (324), Expect = 8e-30
Identities = 90/242 (37%), Positives = 128/242 (52%), Gaps = 1/242 (0%)
Frame = +1
Query: 1 HPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHS 180
HP I++ G R I L+++ GS+ S L K G E + +++Q+L L++LH+
Sbjct: 131 HPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYTRQLLLGLEYLHN 190
Query: 181 QNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVI 360
I+HRD+KG IL+DN IKL F ++K + ++ T A+S GT W APEVI
Sbjct: 191 HAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMT----GAKSMKGTPYWMAPEVI 246
Query: 361 NGEGYGSSADIWSFGAVMVELITGKAPLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALK 537
G+ SADIWS G ++E++TGKAP AI+ +GT K SDA K
Sbjct: 247 LQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPDTLSSDA-K 305
Query: 538 SFVDPCLDPEPLNRPTVKDLMKHEFLNSAMEAD*FT*RVDIFSWIN*NNTYMLPK*INNC 717
F+ CL P RPT +L+KH F+ + T D+ S +N N + LP INN
Sbjct: 306 DFLLKCLQEVPNLRPTASELLKHPFVMGKHKESAST---DLGSVLN-NLSTPLPLQINNT 361
Query: 718 SS 723
S
Sbjct: 362 KS 363
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 124 bits (312), Expect = 2e-28
Identities = 75/206 (36%), Positives = 114/206 (55%), Gaps = 1/206 (0%)
Frame = +1
Query: 1 HPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHS 180
HP I++ G R + I ++++ GS+ S L K G+ EP II ++KQ+L L++LH+
Sbjct: 130 HPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYTKQLLLGLEYLHN 189
Query: 181 QNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVI 360
I+HRD+KG IL+DN I+L F ++K + ++ + A+S GT W APEVI
Sbjct: 190 NGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATV----NGAKSMKGTPYWMAPEVI 245
Query: 361 NGEGYGSSADIWSFGAVMVELITGKAPLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALK 537
G+ SADIWS G ++E+ TGK P F A+ +G + K P S K
Sbjct: 246 LQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIG-RTKAHPPIPEDLSPEAK 304
Query: 538 SFVDPCLDPEPLNRPTVKDLMKHEFL 615
F+ CL EP R + +L++H F+
Sbjct: 305 DFLMKCLHKEPSLRLSATELLQHPFV 330
>sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 618
Score = 113 bits (283), Expect = 4e-25
Identities = 72/208 (34%), Positives = 110/208 (52%), Gaps = 2/208 (0%)
Frame = +1
Query: 1 HPRILKCNGFFRSV--KASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFL 174
H RI++ G R K I ++YM GS++ L GA E ++++QILE + +L
Sbjct: 414 HERIVQYYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGALTENGTRKYTRQILEGVHYL 473
Query: 175 HSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPE 354
HS ILHRD+KG IL D+ ++KL F ++K + +S GT W +PE
Sbjct: 474 HSNMILHRDIKGANILRDSTGNVKLGDFGASKRL---QTICLSGTGMKSVTGTPYWMSPE 530
Query: 355 VINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLKCSDAL 534
VI+G+GYG ADIWS +VE++T K P + AI+++ TQ P SD
Sbjct: 531 VISGQGYGRKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIATQ-PTNPKLPPHVSDYT 589
Query: 535 KSFVDPCLDPEPLNRPTVKDLMKHEFLN 618
+ F+ L RP+ +L++H F++
Sbjct: 590 RDFLKRIFVEAKL-RPSADELLRHMFVH 616
>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 112 bits (281), Expect = 8e-25
Identities = 73/207 (35%), Positives = 111/207 (53%), Gaps = 3/207 (1%)
Frame = +1
Query: 1 HPRILKCNGFFR--SVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFL 174
H RI++ G R + K I ++YM GS++ L GA E ++++QILE + +L
Sbjct: 421 HERIVQYYGCLRDRAEKTLTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYL 480
Query: 175 HSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPD-ASSETFRNHVAESNHGTANWTAP 351
HS I+HRD+KG IL D+ ++KL F ++K S T V GT W +P
Sbjct: 481 HSNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGMRSVT----GTPYWMSP 536
Query: 352 EVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLKCSDA 531
EVI+GEGYG AD+WS G +VE++T K P + AI+++ TQ P S+
Sbjct: 537 EVISGEGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQ-PTNPQLPSHISEH 595
Query: 532 LKSFVDPCLDPEPLNRPTVKDLMKHEF 612
+ F+ + E RP+ ++L+ H F
Sbjct: 596 GRDFLRR-IFVEARQRPSAEELLTHHF 621
>sp|P27636|CDC15_YEAST Cell division control protein 15
Length = 974
Score = 112 bits (281), Expect = 8e-25
Identities = 69/208 (33%), Positives = 99/208 (47%), Gaps = 1/208 (0%)
Frame = +1
Query: 1 HPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQ-KEPSIIRFSKQILEALDFLH 177
H I+K +GF R YI L+Y ANGSLR + ++ E + Q L L +LH
Sbjct: 79 HNNIVKYHGFIRKSYELYILLEYCANGSLRRLISRSSTGLSENESKTYVTQTLLGLKYLH 138
Query: 178 SQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEV 357
+ ++HRD+K ILL + +KL F T N A + GT NW APE+
Sbjct: 139 GEGVIHRDIKAANILLSADNTVKLADF---------GVSTIVNSSALTLAGTLNWMAPEI 189
Query: 358 INGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLKCSDALK 537
+ G + +DIWS GA +VE++T P L Y + + + P S+ LK
Sbjct: 190 LGNRGASTLSDIWSLGATVVEMLTKNPPYHNLTDANIYYAV---ENDTYYPPSSFSEPLK 246
Query: 538 SFVDPCLDPEPLNRPTVKDLMKHEFLNS 621
F+ C RPT L+KH ++NS
Sbjct: 247 DFLSKCFVKNMYKRPTADQLLKHVWINS 274
>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 112 bits (279), Expect = 1e-24
Identities = 71/206 (34%), Positives = 110/206 (53%), Gaps = 2/206 (0%)
Frame = +1
Query: 1 HPRILKCNGFFR--SVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFL 174
H RI++ G R + K I ++YM GS++ L GA E ++++QILE + +L
Sbjct: 421 HERIVQYYGCLRDRAEKILTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYL 480
Query: 175 HSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPE 354
HS I+HRD+KG IL D+ ++KL F ++K + S GT W +PE
Sbjct: 481 HSNMIVHRDIKGANILRDSAGNVKLGDFGASKRL---QTICMSGTGIRSVTGTPYWMSPE 537
Query: 355 VINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLKCSDAL 534
VI+GEGYG AD+WS G +VE++T K P + AI+++ TQ P S+
Sbjct: 538 VISGEGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQ-PTNPQLPSHISEHG 596
Query: 535 KSFVDPCLDPEPLNRPTVKDLMKHEF 612
+ F+ + E RP+ ++L+ H F
Sbjct: 597 RDFLRR-IFVEARQRPSAEELLTHHF 621
>sp|Q13153|PAK1_HUMAN Serine/threonine-protein kinase PAK 1 (p21-activated kinase 1)
(PAK-1) (P65-PAK) (Alpha-PAK)
Length = 545
Score = 112 bits (279), Expect = 1e-24
Identities = 63/192 (32%), Positives = 102/192 (53%), Gaps = 1/192 (0%)
Frame = +1
Query: 52 YIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDN 231
++ ++Y+A GSL + + E I ++ L+AL+FLHS ++HRD+K ILL
Sbjct: 341 WVVMEYLAGGSLTDVVTET-CMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGM 399
Query: 232 NLDIKLCGF-MSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGA 408
+ +KL F A++ P+ S + + GT W APEV+ + YG DIWS G
Sbjct: 400 DGSVKLTDFGFCAQITPEQSKRS-------TMVGTPYWMAPEVVTRKAYGPKVDIWSLGI 452
Query: 409 VMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTV 588
+ +E+I G+ P +A+Y + T E P K S + F++ CLD + R +
Sbjct: 453 MAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLDMDVEKRGSA 512
Query: 589 KDLMKHEFLNSA 624
K+L++H+FL A
Sbjct: 513 KELLQHQFLKIA 524
>sp|Q01389|BCK1_YEAST Serine/threonine-protein kinase BCK1/SLK1/SSP31
Length = 1478
Score = 111 bits (277), Expect = 2e-24
Identities = 68/209 (32%), Positives = 107/209 (51%), Gaps = 5/209 (2%)
Frame = +1
Query: 1 HPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHS 180
H I++ GF + L+Y+A GS+ S + G EP I + Q+L+ L +LHS
Sbjct: 1237 HLNIVQYLGFENKNNIYSLFLEYVAGGSVGSLIRMYGRFDEPLIKHLTTQVLKGLAYLHS 1296
Query: 181 QNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVI 360
+ ILHRD+K +LLD + K+ F ++ D S + + GT W APE++
Sbjct: 1297 KGILHRDMKADNLLLDQDGICKISDFGISRKSKDIYSNS-----DMTMRGTVFWMAPEMV 1351
Query: 361 N-GEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKI----EFAYPLKCS 525
+ +GY + DIWS G +++E+ GK P L A++++G K E PL S
Sbjct: 1352 DTKQGYSAKVDIWSLGCIVLEMFAGKRPWSNLEVVAAMFKIGKSKSAPPIPEDTLPL-IS 1410
Query: 526 DALKSFVDPCLDPEPLNRPTVKDLMKHEF 612
++F+D C + P RPT +L+ H F
Sbjct: 1411 QIGRNFLDACFEINPEKRPTANELLSHPF 1439
>sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (STE20-like kinase MST3) (MST-3)
(Mammalian STE20-like protein kinase 3)
Length = 443
Score = 110 bits (276), Expect = 3e-24
Identities = 71/206 (34%), Positives = 102/206 (49%), Gaps = 2/206 (0%)
Frame = +1
Query: 4 PRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQ 183
P + K G + +I ++Y+ GS L G E I ++IL+ LD+LHS+
Sbjct: 92 PYVTKYYGSYLKDTKLWIIMEYLGGGSALDLLEP-GPLDETQIATILREILKGLDYLHSE 150
Query: 184 NILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVIN 363
+HRD+K +LL + ++KL F A D + RN GT W APEVI
Sbjct: 151 KKIHRDIKAANVLLSEHGEVKLADFGVAGQLTDTQIK--RNTFV----GTPFWMAPEVIK 204
Query: 364 GEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRL--GTQKKIEFAYPLKCSDALK 537
Y S ADIWS G +EL G+ P L + ++ + +E Y S LK
Sbjct: 205 QSAYDSKADIWSLGITAIELARGEPPHSELHPMKVLFLIPKNNPPTLEGNY----SKPLK 260
Query: 538 SFVDPCLDPEPLNRPTVKDLMKHEFL 615
FV+ CL+ EP RPT K+L+KH+F+
Sbjct: 261 EFVEACLNKEPSFRPTAKELLKHKFI 286
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 89,880,675
Number of Sequences: 369166
Number of extensions: 1880101
Number of successful extensions: 7900
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5965
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6329
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6462248555
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)