Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_022_P15
(654 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P04634|LIPG_RAT Gastric triacylglycerol lipase precursor... 136 4e-32
sp|P07098|LIPG_HUMAN Gastric triacylglycerol lipase precurs... 135 7e-32
sp|P80035|LIPG_CANFA Gastric triacylglycerol lipase precurs... 132 6e-31
sp|Q9CPP7|LIPG_MOUSE Gastric triacylglycerol lipase precurs... 129 5e-30
sp|Q29458|LIPG_BOVIN Gastric triacylglycerol lipase precurs... 129 9e-30
sp|P38571|LICH_HUMAN Lysosomal acid lipase/cholesteryl este... 123 5e-28
sp|Q64194|LICH_RAT Lysosomal acid lipase/cholesteryl ester ... 121 1e-27
sp|Q9Z0M5|LICH_MOUSE Lysosomal acid lipase/cholesteryl este... 111 1e-24
sp|O46107|LIP1_DROME Lipase 1 precursor (DmLip1) 84 3e-16
sp|O46108|LIP3_DROME Lipase 3 precursor (DmLip3) 75 2e-13
>sp|P04634|LIPG_RAT Gastric triacylglycerol lipase precursor (Gastric lipase) (GL)
(Lingual lipase)
Length = 395
Score = 136 bits (343), Expect = 4e-32
Identities = 66/160 (41%), Positives = 99/160 (61%), Gaps = 1/160 (0%)
Frame = +3
Query: 12 LFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISHTPA 191
+FG + ++ FL + +C +E L CS +F+ G D KNLN SR VY+ H PA
Sbjct: 224 MFGKKMFLPHTYFDDFLGTEVCSREVLDLLCSNTLFIFCGFDKKNLNVSRFDVYLGHNPA 283
Query: 192 GTSVMNIVHYAQSVDNGNFQMYDFGMEG-NIKRYGTPNPPQFDVKPVGIPTAIFSGGNDT 368
GTSV + +H+AQ V +G FQ +++G N+ Y PP++DV + +P A+++GGND
Sbjct: 284 GTSVQDFLHWAQLVRSGKFQAFNWGSPSQNMLHYNQKTPPEYDVSAMTVPVAVWNGGNDI 343
Query: 369 LADPEDVSRLLGLVGGDVKFQKFISYYNHMDFVWGENAWQ 488
LADP+DV+ LL + ++ F K I YNH+DF+W +A Q
Sbjct: 344 LADPQDVAMLLPKL-SNLLFHKEILAYNHLDFIWAMDAPQ 382
>sp|P07098|LIPG_HUMAN Gastric triacylglycerol lipase precursor (Gastric lipase) (GL)
Length = 398
Score = 135 bits (341), Expect = 7e-32
Identities = 67/160 (41%), Positives = 99/160 (61%), Gaps = 1/160 (0%)
Frame = +3
Query: 12 LFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISHTPA 191
+FG ++ +FLA+ +C +E L CS +F++ G D KN N SRL VY+SH PA
Sbjct: 225 IFGDKIFYPHNFFDQFLATEVCSREMLNLLCSNALFIICGFDSKNFNTSRLDVYLSHNPA 284
Query: 192 GTSVMNIVHYAQSVDNGNFQMYDFGME-GNIKRYGTPNPPQFDVKPVGIPTAIFSGGNDT 368
GTSV N+ H+ Q+V +G FQ YD+G N Y PP ++V + +P A+++GG D
Sbjct: 285 GTSVQNMFHWTQAVKSGKFQAYDWGSPVQNRMHYDQSQPPYYNVTAMNVPIAVWNGGKDL 344
Query: 369 LADPEDVSRLLGLVGGDVKFQKFISYYNHMDFVWGENAWQ 488
LADP+DV LL + ++ + K I +YNH+DF+W +A Q
Sbjct: 345 LADPQDVGLLLPKL-PNLIYHKEIPFYNHLDFIWAMDAPQ 383
>sp|P80035|LIPG_CANFA Gastric triacylglycerol lipase precursor (Gastric lipase) (GL)
Length = 398
Score = 132 bits (333), Expect = 6e-31
Identities = 65/160 (40%), Positives = 100/160 (62%), Gaps = 1/160 (0%)
Frame = +3
Query: 12 LFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISHTPA 191
+FG+ + +FLA+ +C +E + CS +F++ G D NLN SRL VY+SH PA
Sbjct: 225 IFGNKIFYPHHFFDQFLATEVCSRETVDLLCSNALFIICGFDTMNLNMSRLDVYLSHNPA 284
Query: 192 GTSVMNIVHYAQSVDNGNFQMYDFGME-GNIKRYGTPNPPQFDVKPVGIPTAIFSGGNDT 368
GTSV N++H++Q+V +G FQ +D+G N+ Y PP +++ + +P A+++GGND
Sbjct: 285 GTSVQNVLHWSQAVKSGKFQAFDWGSPVQNMMHYHQSMPPYYNLTDMHVPIAVWNGGNDL 344
Query: 369 LADPEDVSRLLGLVGGDVKFQKFISYYNHMDFVWGENAWQ 488
LADP DV LL + + +K I YNH+DF+W +A Q
Sbjct: 345 LADPHDVDLLLSKLPNLIYHRK-IPPYNHLDFIWAMDAPQ 383
>sp|Q9CPP7|LIPG_MOUSE Gastric triacylglycerol lipase precursor (Gastric lipase) (GL)
Length = 395
Score = 129 bits (325), Expect = 5e-30
Identities = 63/160 (39%), Positives = 98/160 (61%), Gaps = 1/160 (0%)
Frame = +3
Query: 12 LFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISHTPA 191
+FG+ M ++ + +FL + +C +E L CS +F+ G D KNLN SR VY+ H PA
Sbjct: 224 IFGNKMFMPHNYLDQFLGTEVCSRELLDLLCSNALFIFCGFDKKNLNVSRFDVYLGHNPA 283
Query: 192 GTSVMNIVHYAQSVDNGNFQMYDFGME-GNIKRYGTPNPPQFDVKPVGIPTAIFSGGNDT 368
GTS ++ H+AQ +G Q Y++G N+ Y PP +DV + +P A+++GG+D
Sbjct: 284 GTSTQDLFHWAQLAKSGKLQAYNWGSPLQNMLHYNQKTPPYYDVSAMTVPIAVWNGGHDI 343
Query: 369 LADPEDVSRLLGLVGGDVKFQKFISYYNHMDFVWGENAWQ 488
LADP+DV+ LL + ++ + K I YNH+DF+W +A Q
Sbjct: 344 LADPQDVAMLLPKL-PNLLYHKEILPYNHLDFIWAMDAPQ 382
>sp|Q29458|LIPG_BOVIN Gastric triacylglycerol lipase precursor (Gastric lipase) (GL)
(Pregastric esterase) (PGE)
Length = 397
Score = 129 bits (323), Expect = 9e-30
Identities = 66/172 (38%), Positives = 98/172 (56%), Gaps = 1/172 (0%)
Frame = +3
Query: 12 LFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISHTPA 191
+FG ++ + +FL +C +E L C +F + G D KN N SRL VYI+H PA
Sbjct: 224 IFGDKMFYPHTFLEQFLGVEMCSRETLDVLCKNALFAITGVDNKNFNMSRLDVYIAHNPA 283
Query: 192 GTSVMNIVHYAQSVDNGNFQMYDFGME-GNIKRYGTPNPPQFDVKPVGIPTAIFSGGNDT 368
GTSV N +H+ Q+V +G FQ +D+G N+ Y P PP +++ + +P A++S ND
Sbjct: 284 GTSVQNTLHWRQAVKSGKFQAFDWGAPYQNLMHYHQPTPPIYNLTAMNVPIAVWSADNDL 343
Query: 369 LADPEDVSRLLGLVGGDVKFQKFISYYNHMDFVWGENAWQNYLPRCVEFHQE 524
LADP+DV LL + ++ + K I YNH+DF+W +A Q V E
Sbjct: 344 LADPQDVDFLLSKL-SNLIYHKEIPNYNHLDFIWAMDAPQEVYNEIVSLMAE 394
>sp|P38571|LICH_HUMAN Lysosomal acid lipase/cholesteryl ester hydrolase precursor (LAL)
(Acid cholesteryl ester hydrolase) (Sterol esterase)
(Lipase A) (Cholesteryl esterase)
Length = 399
Score = 123 bits (308), Expect = 5e-28
Identities = 65/164 (39%), Positives = 101/164 (61%), Gaps = 2/164 (1%)
Frame = +3
Query: 9 NLFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISHTP 188
+LFG + S K+L + +C L + C + FLL G + +NLN SR+ VY +H+P
Sbjct: 226 DLFGDKEFLPQSAFLKWLGTHVCTHVILKELCGNLCFLLCGFNERNLNMSRVDVYTTHSP 285
Query: 189 AGTSVMNIVHYAQSVDNGNFQMYDFGMEG-NIKRYGTPNPPQFDVKPVGIPTAIFSGGND 365
AGTSV N++H++Q+V FQ +D+G N Y PP ++VK + +PTA++SGG+D
Sbjct: 286 AGTSVQNMLHWSQAVKFQKFQAFDWGSSAKNYFHYNQSYPPTYNVKDMLVPTAVWSGGHD 345
Query: 366 TLADPEDVSRLLGLVGGDVKFQKFISYYNHMDFVWGENA-WQNY 494
LAD DV+ LL + ++ F + I + H+DF+WG +A W+ Y
Sbjct: 346 WLADVYDVNILLTQI-TNLVFHESIPEWEHLDFIWGLDAPWRLY 388
>sp|Q64194|LICH_RAT Lysosomal acid lipase/cholesteryl ester hydrolase precursor (LAL)
(Acid cholesteryl ester hydrolase) (Sterol esterase)
(Lipase A) (Cholesteryl esterase)
Length = 397
Score = 121 bits (304), Expect = 1e-27
Identities = 64/166 (38%), Positives = 101/166 (60%), Gaps = 2/166 (1%)
Frame = +3
Query: 3 LLNLFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISH 182
L +LFG + + S + K+L++ IC + + C+ + FL+ G + KNLN SR+ VY +H
Sbjct: 222 LEDLFGQKQFLPQSAMVKWLSTHICTHVIMKELCANIFFLICGFNEKNLNMSRVDVYTTH 281
Query: 183 TPAGTSVMNIVHYAQSVDNGNFQMYDFG-MEGNIKRYGTPNPPQFDVKPVGIPTAIFSGG 359
PAGTSV N+VH+ Q V Q +D+G + N Y PP + +K + +PTA++SGG
Sbjct: 282 CPAGTSVQNMVHWTQVVKYHKLQAFDWGSSDKNYFHYNQSYPPLYSIKDMQLPTALWSGG 341
Query: 360 NDTLADPEDVSRLLGLVGGDVKFQKFISYYNHMDFVWGENA-WQNY 494
D LAD D++ LL + + + K I ++H+DF+WG +A W+ Y
Sbjct: 342 KDWLADTSDINILLTEI-PTLVYHKNIPEWDHLDFIWGLDAPWRLY 386
>sp|Q9Z0M5|LICH_MOUSE Lysosomal acid lipase/cholesteryl ester hydrolase precursor (LAL)
(Acid cholesteryl ester hydrolase) (Sterol esterase)
(Lipase A) (Cholesteryl esterase)
Length = 397
Score = 111 bits (278), Expect = 1e-24
Identities = 60/166 (36%), Positives = 97/166 (58%), Gaps = 2/166 (1%)
Frame = +3
Query: 3 LLNLFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISH 182
L ++FG + + S + K+L+ +C + + C+ V FLL G + KNLN SR+ VY +H
Sbjct: 222 LKDMFGQKQFLPQSAMLKWLSIHVCTHVIMKELCANVFFLLCGFNEKNLNMSRVDVYTTH 281
Query: 183 TPAGTSVMNIVHYAQSVDNGNFQMYDFG-MEGNIKRYGTPNPPQFDVKPVGIPTAIFSGG 359
PA V N++H+ Q Q +D+G E N Y PP +++K + +PTA++SGG
Sbjct: 282 CPAELLVQNMLHWGQVFKYRKLQAFDWGSSEKNYFHYNQSFPPSYNIKNMRLPTALWSGG 341
Query: 360 NDTLADPEDVSRLLGLVGGDVKFQKFISYYNHMDFVWGENA-WQNY 494
D LAD D++ LL + + + K I ++H+DF+WG +A W+ Y
Sbjct: 342 RDWLADINDITILLTQI-PKLVYHKNIPEWDHLDFIWGLDAPWKLY 386
>sp|O46107|LIP1_DROME Lipase 1 precursor (DmLip1)
Length = 439
Score = 84.0 bits (206), Expect = 3e-16
Identities = 48/151 (31%), Positives = 74/151 (49%), Gaps = 3/151 (1%)
Frame = +3
Query: 27 RVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISHTPAGTSVM 206
R M N E F+FL E + C E +F + G + N PV + H PAG +
Sbjct: 254 REMFNGE-FRFLCRMT---EETERLCIEAVFGIVGRNWNEFNRKMFPVILGHYPAGVAAK 309
Query: 207 NIVHYAQSVDNGNFQMYDFGMEGNIKRYGTPNPPQFDVKPVGIPTAIFSGGNDTLADPED 386
+ H+ Q + +G F Y + N++ Y PP++++ V +PT ++ ND L P+D
Sbjct: 310 QVKHFIQIIKSGRFAPYSYSSNKNMQLYRDHLPPRYNLSLVTVPTFVYYSTNDLLCHPKD 369
Query: 387 VSRL---LGLVGGDVKFQKFISYYNHMDFVW 470
V + LG V G K+ +NHMDF+W
Sbjct: 370 VESMCDDLGNVTG--KYLVPQKEFNHMDFLW 398
>sp|O46108|LIP3_DROME Lipase 3 precursor (DmLip3)
Length = 394
Score = 74.7 bits (182), Expect = 2e-13
Identities = 43/132 (32%), Positives = 69/132 (52%), Gaps = 5/132 (3%)
Frame = +3
Query: 102 CSEVIFLLAGHDIKNLNESRLPVYISHTPAGTSVMNIVHYAQSVDNGNFQMYDFGMEGNI 281
C+ IFL+ G+D + L+ L + +PAG SV +H+ Q ++G F+ +D+ N
Sbjct: 251 CANEIFLIGGYDTEQLDYELLEHIKATSPAGASVNQNLHFCQEYNSGKFRKFDYTALRNP 310
Query: 282 KRYGTPNPPQFDVKPVGIPTAIFSGGNDTLADPEDVSRL---LGLVGGD--VKFQKFISY 446
YG+ PP + +K P ++ G ND + D DV +L L + D V F+K
Sbjct: 311 YEYGSYFPPDYKLKNAKAPVLLYYGANDWMCDVSDVRKLRDELPNMALDYLVPFEK---- 366
Query: 447 YNHMDFVWGENA 482
+ H+DF+WG A
Sbjct: 367 WAHLDFIWGTEA 378
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,742,575
Number of Sequences: 369166
Number of extensions: 1934003
Number of successful extensions: 4938
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4740
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4919
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5413810770
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)