Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_022_O08
(744 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q02253|MMSA_RAT Methylmalonate-semialdehyde dehydrogenas... 263 4e-70
sp|Q02252|MMSA_HUMAN Methylmalonate-semialdehyde dehydrogen... 261 1e-69
sp|Q07536|MMSA_BOVIN Methylmalonate-semialdehyde dehydrogen... 257 2e-68
sp|P52713|MMSA_CAEEL Probable methylmalonate-semialdehyde d... 254 1e-67
sp|P28810|MMSA_PSEAE Methylmalonate-semialdehyde dehydrogen... 174 2e-43
sp|P42412|MMSA_BACSU Probable methylmalonate-semialdehyde d... 171 2e-42
sp|P42236|ALDH1_BACSU Probable aldehyde dehydrogenase ycbD 119 1e-26
sp|P24549|AL1A1_MOUSE Retinal dehydrogenase 1 (RalDH1) (RAL... 115 1e-25
sp|P51647|AL1A1_RAT Retinal dehydrogenase 1 (RalDH1) (RALDH... 114 2e-25
sp|Q8MI17|AL1A1_RABIT Retinal dehydrogenase 1 (RalDH1) (RAL... 114 4e-25
>sp|Q02253|MMSA_RAT Methylmalonate-semialdehyde dehydrogenase [acylating],
mitochondrial precursor (MMSDH) (Malonate-semialdehyde
dehydrogenase [acylating])
Length = 535
Score = 263 bits (672), Expect = 4e-70
Identities = 120/196 (61%), Positives = 161/196 (82%)
Frame = +3
Query: 126 STGIPKTKLFINGEFVESQATEWIDVLNPATNEVVTLVPKSTNSEMESAVKSCIKAFESW 305
S+ +P KLFI+G+FVES++ +WID+ NPATNEVV VP+ST +EME+AV +C +AF +W
Sbjct: 34 SSSVPTVKLFIDGKFVESKSDKWIDIHNPATNEVVGRVPQSTKAEMEAAVAACKRAFPAW 93
Query: 306 SLRSVLNRQQYMFKLQELIKRDLKKIAKNITIQQGKTFGDAEGDVLRGLQVVEHCCSITN 485
+ S+L+RQQ + + Q+LIK +LK+IA+ IT++QGKT DAEGDV RGLQVVEH CS+T+
Sbjct: 94 ADTSILSRQQVLLRYQQLIKENLKEIARLITLEQGKTLADAEGDVFRGLQVVEHACSVTS 153
Query: 486 LQLGETMPGVAKDLDIVSYKEPIGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSER 665
L LGETMP + KD+D+ SY+ P+GV AGI PFNFPAMIPLWMFP+++VCGNT ++KPSER
Sbjct: 154 LMLGETMPSITKDMDLYSYRLPLGVCAGIAPFNFPAMIPLWMFPMAMVCGNTFLMKPSER 213
Query: 666 DPGAAMMLMELCVEAG 713
PGA M+L +L ++G
Sbjct: 214 VPGATMLLAKLLQDSG 229
>sp|Q02252|MMSA_HUMAN Methylmalonate-semialdehyde dehydrogenase [acylating],
mitochondrial precursor (MMSDH) (Malonate-semialdehyde
dehydrogenase [acylating])
Length = 535
Score = 261 bits (668), Expect = 1e-69
Identities = 118/196 (60%), Positives = 160/196 (81%)
Frame = +3
Query: 126 STGIPKTKLFINGEFVESQATEWIDVLNPATNEVVTLVPKSTNSEMESAVKSCIKAFESW 305
S+ +P KLFI G+FVES++ +WID+ NPATNEV+ VP++T +EM++A+ SC +AF +W
Sbjct: 34 SSSVPTVKLFIGGKFVESKSDKWIDIHNPATNEVIGRVPQATKAEMDAAIASCKRAFPAW 93
Query: 306 SLRSVLNRQQYMFKLQELIKRDLKKIAKNITIQQGKTFGDAEGDVLRGLQVVEHCCSITN 485
+ SVL+RQQ + + Q+LIK +LK+IAK IT++QGKT DAEGDV RGLQVVEH CS+T+
Sbjct: 94 ADTSVLSRQQVLLRYQQLIKENLKEIAKLITLEQGKTLADAEGDVFRGLQVVEHACSVTS 153
Query: 486 LQLGETMPGVAKDLDIVSYKEPIGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSER 665
L +GETMP + KD+D+ SY+ P+GV AGI PFNFPAMIPLWMFP+++VCGNT ++KPSER
Sbjct: 154 LMMGETMPSITKDMDLYSYRLPLGVCAGIAPFNFPAMIPLWMFPMAMVCGNTFLMKPSER 213
Query: 666 DPGAAMMLMELCVEAG 713
PGA M+L +L ++G
Sbjct: 214 VPGATMLLAKLLQDSG 229
>sp|Q07536|MMSA_BOVIN Methylmalonate-semialdehyde dehydrogenase [acylating],
mitochondrial precursor (MMSDH) (Malonate-semialdehyde
dehydrogenase [acylating])
Length = 537
Score = 257 bits (657), Expect = 2e-68
Identities = 115/196 (58%), Positives = 159/196 (81%)
Frame = +3
Query: 126 STGIPKTKLFINGEFVESQATEWIDVLNPATNEVVTLVPKSTNSEMESAVKSCIKAFESW 305
S+ +P KLFI+G+F+ES++ +WID+ NPATNEV+ VP+ST +EM++AV SC + F +W
Sbjct: 36 SSSVPTVKLFIDGKFIESKSDKWIDIHNPATNEVIGRVPESTKAEMDAAVSSCKRTFPAW 95
Query: 306 SLRSVLNRQQYMFKLQELIKRDLKKIAKNITIQQGKTFGDAEGDVLRGLQVVEHCCSITN 485
+ S+L+RQQ + + Q+LIK +LK+IA+ I ++QGKT DAEGDV RGLQVVEH CS+T+
Sbjct: 96 ADTSILSRQQVLLRYQQLIKENLKEIARLIMLEQGKTLADAEGDVFRGLQVVEHACSVTS 155
Query: 486 LQLGETMPGVAKDLDIVSYKEPIGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSER 665
L LG+TMP + KD+D+ SY+ P+GV AGI PFNFPAMIPLWMFP+++VCGNT ++KPSER
Sbjct: 156 LMLGDTMPSITKDMDLYSYRLPLGVCAGIAPFNFPAMIPLWMFPMAMVCGNTFLMKPSER 215
Query: 666 DPGAAMMLMELCVEAG 713
PGA M+L +L ++G
Sbjct: 216 VPGATMLLAKLFQDSG 231
>sp|P52713|MMSA_CAEEL Probable methylmalonate-semialdehyde dehydrogenase [acylating],
mitochondrial precursor (MMSDH) (Malonate-semialdehyde
dehydrogenase [acylating])
Length = 523
Score = 254 bits (650), Expect = 1e-67
Identities = 119/192 (61%), Positives = 156/192 (81%)
Frame = +3
Query: 138 PKTKLFINGEFVESQATEWIDVLNPATNEVVTLVPKSTNSEMESAVKSCIKAFESWSLRS 317
P KL+I+G+ VES+ T+++++ NPATNEV+ +VP +T +EM++AV S AF +W S
Sbjct: 28 PTVKLWIDGQAVESKTTDFVELTNPATNEVIAMVPNATQAEMQAAVDSAKNAFNTWKNTS 87
Query: 318 VLNRQQYMFKLQELIKRDLKKIAKNITIQQGKTFGDAEGDVLRGLQVVEHCCSITNLQLG 497
L RQQ MFKLQ LIKRD+KK+A++ITI+QGKT DAEGDV RGLQVVEH CS+ +L +G
Sbjct: 88 PLTRQQCMFKLQALIKRDMKKLAESITIEQGKTLPDAEGDVSRGLQVVEHACSVPSLMMG 147
Query: 498 ETMPGVAKDLDIVSYKEPIGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGA 677
ET+P V++D+D SY+ P+GVTAGI PFNFPAMIPLWMFP++L GNTM++KPSE+DPGA
Sbjct: 148 ETLPNVSRDMDTHSYRIPLGVTAGICPFNFPAMIPLWMFPVALATGNTMVIKPSEQDPGA 207
Query: 678 AMMLMELCVEAG 713
A +L+EL EAG
Sbjct: 208 AQLLVELAKEAG 219
>sp|P28810|MMSA_PSEAE Methylmalonate-semialdehyde dehydrogenase [acylating] (MMSDH)
Length = 497
Score = 174 bits (441), Expect = 2e-43
Identities = 86/186 (46%), Positives = 121/186 (65%)
Frame = +3
Query: 156 INGEFVESQATEWIDVLNPATNEVVTLVPKSTNSEMESAVKSCIKAFESWSLRSVLNRQQ 335
I G FVE + I V NP N + + ++ ++E AV S + F SW V R +
Sbjct: 9 IAGAFVEGLGAQRIPVSNPLDNSTLAEIACASAEQVEQAVASARETFASWKETPVSERAR 68
Query: 336 YMFKLQELIKRDLKKIAKNITIQQGKTFGDAEGDVLRGLQVVEHCCSITNLQLGETMPGV 515
M + Q L+K ++AK ++ + GKTF DA+GDV RG++VVEH C++ +L +GET+ V
Sbjct: 69 VMLRYQALLKEHHDELAKIVSSELGKTFEDAKGDVWRGIEVVEHACNVPSLLMGETVENV 128
Query: 516 AKDLDIVSYKEPIGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGAAMMLME 695
A+++D S +P+GV GITPFNFPAMIPLWMFPL++ CGN ILKPSE+ P ++ L E
Sbjct: 129 ARNIDTYSITQPLGVCVGITPFNFPAMIPLWMFPLAIACGNAFILKPSEQVPLTSVRLAE 188
Query: 696 LCVEAG 713
L +EAG
Sbjct: 189 LFLEAG 194
>sp|P42412|MMSA_BACSU Probable methylmalonate-semialdehyde dehydrogenase [acylating]
(MMSDH)
Length = 487
Score = 171 bits (433), Expect = 2e-42
Identities = 83/193 (43%), Positives = 128/193 (66%)
Frame = +3
Query: 135 IPKTKLFINGEFVESQATEWIDVLNPATNEVVTLVPKSTNSEMESAVKSCIKAFESWSLR 314
I K K +INGE+VES+ ++ DV+NPAT EV+ VP ST +++ A ++ +AF++WS
Sbjct: 4 IRKLKNYINGEWVESKTDQYEDVVNPATKEVLCQVPISTKEDIDYAAQTAAEAFKTWSKV 63
Query: 315 SVLNRQQYMFKLQELIKRDLKKIAKNITIQQGKTFGDAEGDVLRGLQVVEHCCSITNLQL 494
+V R + +F Q+L+ + +++A ITI+ GK +A G+V RG++ VE +L +
Sbjct: 64 AVPRRARILFNFQQLLSQHKEELAHLITIENGKNTKEALGEVGRGIENVEFAAGAPSLMM 123
Query: 495 GETMPGVAKDLDIVSYKEPIGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPG 674
G+++ +A D++ +Y+ PIGV GI PFNFP M+P WMFP+++ GNT ILKPSER P
Sbjct: 124 GDSLASIATDVEAANYRYPIGVVGGIAPFNFPMMVPCWMFPMAIALGNTFILKPSERTPL 183
Query: 675 AAMMLMELCVEAG 713
L+EL +AG
Sbjct: 184 LTEKLVELFEKAG 196
>sp|P42236|ALDH1_BACSU Probable aldehyde dehydrogenase ycbD
Length = 488
Score = 119 bits (297), Expect = 1e-26
Identities = 62/188 (32%), Positives = 109/188 (57%), Gaps = 1/188 (0%)
Frame = +3
Query: 153 FINGEFVESQATEWIDVLNPA-TNEVVTLVPKSTNSEMESAVKSCIKAFESWSLRSVLNR 329
FINGE+V+SQ+ + + V NPA N++V V ST ++E AV + +A +W + R
Sbjct: 13 FINGEWVKSQSGDMVKVENPADVNDIVGYVQNSTAEDVERAVTAANEAKTAWRKLTGAER 72
Query: 330 QQYMFKLQELIKRDLKKIAKNITIQQGKTFGDAEGDVLRGLQVVEHCCSITNLQLGETMP 509
QY++K +++++ L++IA T + GKT +A+G+ RG+ ++ + + G+ +P
Sbjct: 73 GQYLYKTADIMEQRLEEIAACATREMGKTLPEAKGETARGIAILRYYAGEGMRKTGDVIP 132
Query: 510 GVAKDLDIVSYKEPIGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKPSERDPGAAMML 689
KD + + + P+GV I+P+NFP IP+W +LV GNT+++KP+ +
Sbjct: 133 STDKDALMFTTRVPLGVVGVISPWNFPVAIPIWKMAPALVYGNTVVIKPATETAVTCAKI 192
Query: 690 MELCVEAG 713
+ EAG
Sbjct: 193 IACFEEAG 200
>sp|P24549|AL1A1_MOUSE Retinal dehydrogenase 1 (RalDH1) (RALDH 1) (Aldehyde dehydrogenase
family 1 member A1) (Aldehyde dehydrogenase, cytosolic)
(ALHDII) (ALDH-E1)
Length = 501
Score = 115 bits (288), Expect = 1e-25
Identities = 71/200 (35%), Positives = 113/200 (56%), Gaps = 6/200 (3%)
Frame = +3
Query: 135 IPKTKLFINGEFVESQATEWIDVLNPATNEVVTLVPKSTNSEMESAVKSCIKAFE---SW 305
I TK+FIN E+ S + + VLNPAT EV+ V + ++++ AVK+ +AF+ W
Sbjct: 18 IQHTKIFINNEWHNSVSGKKFPVLNPATEEVICHVEEGDKADVDKAVKAARQAFQIGSPW 77
Query: 306 SLRSVLNRQQYMFKLQELIKRDLKKIAKNITIQQGKTFGDAEGDVLRG-LQVVEHCCSIT 482
R + + KL +L++RD +A + GK F +A L G ++ +++C
Sbjct: 78 RTMDASERGRLLNKLADLMERDRLLLATMEALNGGKVFANAYLSDLGGCIKALKYCAGWA 137
Query: 483 NLQLGETMPGVAKDLDIVSY--KEPIGVTAGITPFNFPAMIPLWMFPLSLVCGNTMILKP 656
+ G+T+P D DI +Y +EPIGV I P+NFP ++ +W +L CGNT+++KP
Sbjct: 138 DKIHGQTIPS---DGDIFTYTRREPIGVCGQIIPWNFPMLMFIWKIGPALSCGNTVVVKP 194
Query: 657 SERDPGAAMMLMELCVEAGF 716
+E+ P A+ L L EAGF
Sbjct: 195 AEQTPLTALHLASLIKEAGF 214
>sp|P51647|AL1A1_RAT Retinal dehydrogenase 1 (RalDH1) (RALDH 1) (Aldehyde dehydrogenase
family 1 member A1) (Aldehyde dehydrogenase, cytosolic)
(ALHDII) (ALDH-E1)
Length = 501
Score = 114 bits (286), Expect = 2e-25
Identities = 71/205 (34%), Positives = 116/205 (56%), Gaps = 6/205 (2%)
Frame = +3
Query: 120 LLSTGIPKTKLFINGEFVESQATEWIDVLNPATNEVVTLVPKSTNSEMESAVKSCIKAFE 299
L + I TK+FIN E+ +S + + VLNPAT EV+ V + ++++ AVK+ +AF+
Sbjct: 13 LANLKIQHTKIFINNEWHDSVSGKKFPVLNPATEEVICHVEEGDKADVDKAVKAARQAFQ 72
Query: 300 ---SWSLRSVLNRQQYMFKLQELIKRDLKKIAKNITIQQGKTFGDAEGDVLRG-LQVVEH 467
W R + + KL +L++RD +A I GK F +A L G ++ +++
Sbjct: 73 IGSPWRTMDASERGRLLNKLADLMERDRLLLATIEAINGGKVFANAYLSDLGGSIKALKY 132
Query: 468 CCSITNLQLGETMPGVAKDLDIVSY--KEPIGVTAGITPFNFPAMIPLWMFPLSLVCGNT 641
C + G+T+P D DI ++ +EPIGV I P+NFP ++ +W +L CGNT
Sbjct: 133 CAGWADKIHGQTIPS---DGDIFTFTRREPIGVCGQIIPWNFPLLMFIWKIGPALSCGNT 189
Query: 642 MILKPSERDPGAAMMLMELCVEAGF 716
+++KP+E+ P A+ + L EAGF
Sbjct: 190 VVVKPAEQTPLTALHMASLIKEAGF 214
>sp|Q8MI17|AL1A1_RABIT Retinal dehydrogenase 1 (RalDH1) (RALDH 1) (Aldehyde dehydrogenase
family 1 member A1) (Aldehyde dehydrogenase, cytosolic)
(ALHDII) (ALDH-E1)
Length = 496
Score = 114 bits (284), Expect = 4e-25
Identities = 71/205 (34%), Positives = 113/205 (55%), Gaps = 6/205 (2%)
Frame = +3
Query: 120 LLSTGIPKTKLFINGEFVESQATEWIDVLNPATNEVVTLVPKSTNSEMESAVKSCIKAFE 299
L + I TK+FIN E+ +S + + VLNPAT E + L+ + ++++ AVK+ +AF+
Sbjct: 8 LTNLKIQYTKIFINNEWHDSVSGKKFPVLNPATEEQICLIEEGDKADVDKAVKAARQAFQ 67
Query: 300 ---SWSLRSVLNRQQYMFKLQELIKRDLKKIAKNITIQQGKTFGDAEGDVLRG-LQVVEH 467
W R + ++KL +LI+RD +A ++ GK F +A L G ++ + +
Sbjct: 68 IGSPWRTMDASERGRLLYKLADLIERDRLLLATMESLNAGKLFPNAYLMDLGGCIKTLRY 127
Query: 468 CCSITNLQLGETMPGVAKDLDIVSYK--EPIGVTAGITPFNFPAMIPLWMFPLSLVCGNT 641
C + G TMP D D Y EP+GV I P+NFP ++ +W +L CGNT
Sbjct: 128 CAGWADKIQGRTMP---MDGDFFCYTRHEPVGVCGQIIPWNFPLVMLIWKIGPALSCGNT 184
Query: 642 MILKPSERDPGAAMMLMELCVEAGF 716
+I+KP+E+ P A+ + L EAGF
Sbjct: 185 VIVKPAEQTPLTALHVASLIKEAGF 209
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 87,505,453
Number of Sequences: 369166
Number of extensions: 1769058
Number of successful extensions: 5100
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4812
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4969
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6728100400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)