Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_022_O05
(332 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P75973|YMFJ_ECOLI Hypothetical protein ymfF 30 2.3
sp|Q88E14|TRMA_PSEPK tRNA (Uracil-5-)-methyltransferase (tR... 28 6.6
sp|Q00151|VG29_ICHV1 Hypothetical gene 29 protein 28 8.7
>sp|P75973|YMFJ_ECOLI Hypothetical protein ymfF
Length = 94
Score = 29.6 bits (65), Expect = 2.3
Identities = 17/63 (26%), Positives = 27/63 (42%), Gaps = 1/63 (1%)
Frame = -2
Query: 274 RNCSSMMPNLFETTNYGENRAPPTPCDGDQIGGAQDC*YPGYHEPKV-TCRFNLTGNRPV 98
+ C + P E N+ N P PC+G+++ YH+ V R L G R
Sbjct: 33 KTCGAQAPGRTEFMNFMANTLTPLPCNGEKVSRV-------YHDTMVKALRIELDGLRRK 85
Query: 97 III 89
I++
Sbjct: 86 IVM 88
>sp|Q88E14|TRMA_PSEPK tRNA (Uracil-5-)-methyltransferase
(tRNA(M-5-U54)-methyltransferase) (RUMT)
Length = 361
Score = 28.1 bits (61), Expect = 6.6
Identities = 17/55 (30%), Positives = 27/55 (49%), Gaps = 1/55 (1%)
Frame = +1
Query: 127 YK*LLARDILDINNLVHLLSDRHHRAWEARDSRHNSLFQISLASYLSSFVMI-MC 288
+K +L D + ++ L R AW+A + N LFQ+ + L+ MI MC
Sbjct: 69 HKAILIDDFPIASERINALMPRLKAAWQASEELGNRLFQVEFLTTLAGDAMITMC 123
>sp|Q00151|VG29_ICHV1 Hypothetical gene 29 protein
Length = 253
Score = 27.7 bits (60), Expect = 8.7
Identities = 11/27 (40%), Positives = 18/27 (66%)
Frame = +1
Query: 151 ILDINNLVHLLSDRHHRAWEARDSRHN 231
I D+N L H + + HHR+ E+R+ + N
Sbjct: 29 IEDVNALAHRIENIHHRSQESREMQLN 55
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,815,427
Number of Sequences: 369166
Number of extensions: 700158
Number of successful extensions: 1535
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 1517
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1535
length of database: 68,354,980
effective HSP length: 78
effective length of database: 53,945,650
effective search space used: 1726260800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)