Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_022_N20
(448 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P00940|TPIS_CHICK Triosephosphate isomerase (TIM) (Trios... 207 8e-54
sp|Q589R5|TPIS_ORYLA Triosephosphate isomerase (TIM) (Trios... 205 4e-53
sp|P48501|TPIS_SCHMA Triosephosphate isomerase (TIM) (Trios... 198 4e-51
sp|P54714|TPIS_CANFA Triosephosphate isomerase (TIM) (Trios... 195 4e-50
sp|Q60HC9|TPIS_MACFA Triosephosphate isomerase (TIM) (Trios... 193 1e-49
sp|P60174|TPIS_HUMAN Triosephosphate isomerase (TIM) (Trios... 193 1e-49
sp|P00941|TPIS_LATCH Triosephosphate isomerase (TIM) (Trios... 193 2e-49
sp|P00939|TPIS_RABIT Triosephosphate isomerase (TIM) (Trios... 192 4e-49
sp|P48500|TPIS_RAT Triosephosphate isomerase (TIM) (Triose-... 192 4e-49
sp|P17751|TPIS_MOUSE Triosephosphate isomerase (TIM) (Trios... 188 5e-48
>sp|P00940|TPIS_CHICK Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 248
Score = 207 bits (527), Expect = 8e-54
Identities = 102/139 (73%), Positives = 112/139 (80%)
Frame = +2
Query: 32 RKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKIGVSA 211
RKFFVGGNWKMNGDK L ++ ++L AKL TEVV PSIYLDF RQKL AKIGV+A
Sbjct: 4 RKFFVGGNWKMNGDKKSLGELIHTLNGAKLSADTEVVCGAPSIYLDFARQKLDAKIGVAA 63
Query: 212 QNCYKIPKGAFTGEISPAMIKDNMCDWVILGHSERRHVFGESDHLIGEKVKHALESGLSI 391
QNCYK+PKGAFTGEISPAMIKD WVILGHSERRHVFGESD LIG+KV HAL GL +
Sbjct: 64 QNCYKVPKGAFTGEISPAMIKDIGAAWVILGHSERRHVFGESDELIGQKVAHALAEGLGV 123
Query: 392 IPCIGEKLDERESNKTNEV 448
I CIGEKLDERE+ T +V
Sbjct: 124 IACIGEKLDEREAGITEKV 142
>sp|Q589R5|TPIS_ORYLA Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 248
Score = 205 bits (521), Expect = 4e-53
Identities = 98/140 (70%), Positives = 115/140 (82%)
Frame = +2
Query: 29 QRKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKIGVS 208
QR+FFVGGNWKMNG+K+ L ++ ++L A LHE TEVV A PSIYLDF R L ++I V+
Sbjct: 3 QRRFFVGGNWKMNGNKESLEELMSTLNTASLHEDTEVVCAAPSIYLDFARSGLDSRISVA 62
Query: 209 AQNCYKIPKGAFTGEISPAMIKDNMCDWVILGHSERRHVFGESDHLIGEKVKHALESGLS 388
AQNCYK+ KGAFTGEISPAMIKD DWVILGHSERRHVFGE+D LIG+KV HALES LS
Sbjct: 63 AQNCYKVAKGAFTGEISPAMIKDCGADWVILGHSERRHVFGENDELIGQKVAHALESDLS 122
Query: 389 IIPCIGEKLDERESNKTNEV 448
+I CIGEKL+ERE+ T +V
Sbjct: 123 VIACIGEKLEEREAGTTEDV 142
>sp|P48501|TPIS_SCHMA Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 253
Score = 198 bits (504), Expect = 4e-51
Identities = 94/143 (65%), Positives = 112/143 (78%)
Frame = +2
Query: 20 MSNQRKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKI 199
MS RKFFVGGNWKMNG +D+ K+ L A + TEV++APPS++L +R+ L +I
Sbjct: 1 MSGSRKFFVGGNWKMNGSRDDNDKLLKLLSEAHFDDNTEVLIAPPSVFLHEIRKSLKKEI 60
Query: 200 GVSAQNCYKIPKGAFTGEISPAMIKDNMCDWVILGHSERRHVFGESDHLIGEKVKHALES 379
V+AQNCYK+ KGAFTGEISPAMI+D CDWVILGHSERR++FGESD LI EKV+HAL
Sbjct: 61 HVAAQNCYKVSKGAFTGEISPAMIRDIGCDWVILGHSERRNIFGESDELIAEKVQHALAE 120
Query: 380 GLSIIPCIGEKLDERESNKTNEV 448
GLS+I CIGE L ERESNKT EV
Sbjct: 121 GLSVIACIGETLSERESNKTEEV 143
>sp|P54714|TPIS_CANFA Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 249
Score = 195 bits (495), Expect = 4e-50
Identities = 95/143 (66%), Positives = 109/143 (76%)
Frame = +2
Query: 20 MSNQRKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKI 199
M+ RKFFVGGNWKMNG K L ++ +L AK+ TEVV APP+ Y+DF RQKL AKI
Sbjct: 1 MAPSRKFFVGGNWKMNGRKKNLGELITTLNAAKVPADTEVVCAPPTAYIDFARQKLDAKI 60
Query: 200 GVSAQNCYKIPKGAFTGEISPAMIKDNMCDWVILGHSERRHVFGESDHLIGEKVKHALES 379
V+AQNCYK+ GAFTGEISP MIKD WV+LGHSERRHVFGESD LIG+KV HAL
Sbjct: 61 AVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAE 120
Query: 380 GLSIIPCIGEKLDERESNKTNEV 448
GL +I CIGEKLDERE+ T +V
Sbjct: 121 GLGVIACIGEKLDEREAGITEKV 143
>sp|Q60HC9|TPIS_MACFA Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
sp|P15426|TPIS_MACMU Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 249
Score = 193 bits (491), Expect = 1e-49
Identities = 94/143 (65%), Positives = 108/143 (75%)
Frame = +2
Query: 20 MSNQRKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKI 199
M+ RKFFVGGNWKMNG K L ++ +L AK+ TEVV APP+ Y+DF RQKL KI
Sbjct: 1 MAPSRKFFVGGNWKMNGRKQNLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKI 60
Query: 200 GVSAQNCYKIPKGAFTGEISPAMIKDNMCDWVILGHSERRHVFGESDHLIGEKVKHALES 379
V+AQNCYK+ GAFTGEISP MIKD WV+LGHSERRHVFGESD LIG+KV HAL
Sbjct: 61 AVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAE 120
Query: 380 GLSIIPCIGEKLDERESNKTNEV 448
GL +I CIGEKLDERE+ T +V
Sbjct: 121 GLGVIACIGEKLDEREAGITEKV 143
>sp|P60174|TPIS_HUMAN Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
sp|P60175|TPIS_PANTR Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 249
Score = 193 bits (491), Expect = 1e-49
Identities = 94/143 (65%), Positives = 108/143 (75%)
Frame = +2
Query: 20 MSNQRKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKI 199
M+ RKFFVGGNWKMNG K L ++ +L AK+ TEVV APP+ Y+DF RQKL KI
Sbjct: 1 MAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKI 60
Query: 200 GVSAQNCYKIPKGAFTGEISPAMIKDNMCDWVILGHSERRHVFGESDHLIGEKVKHALES 379
V+AQNCYK+ GAFTGEISP MIKD WV+LGHSERRHVFGESD LIG+KV HAL
Sbjct: 61 AVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAE 120
Query: 380 GLSIIPCIGEKLDERESNKTNEV 448
GL +I CIGEKLDERE+ T +V
Sbjct: 121 GLGVIACIGEKLDEREAGITEKV 143
>sp|P00941|TPIS_LATCH Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 247
Score = 193 bits (490), Expect = 2e-49
Identities = 94/139 (67%), Positives = 106/139 (76%)
Frame = +2
Query: 32 RKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKIGVSA 211
RKFFVGGNWKMNGDK L ++ +L AK+ T E+V APP YLDF R K+ K GV+A
Sbjct: 3 RKFFVGGNWKMNGDKKSLGELIQTLNAAKVPFTGEIVCAPPEAYLDFARLKVDPKFGVAA 62
Query: 212 QNCYKIPKGAFTGEISPAMIKDNMCDWVILGHSERRHVFGESDHLIGEKVKHALESGLSI 391
QNCYK+ KGAFTGEISPAMIKD WVILGHSERRHVFGESD LIG+KV HAL GL +
Sbjct: 63 QNCYKVSKGAFTGEISPAMIKDCGVTWVILGHSERRHVFGESDELIGQKVSHALSEGLGV 122
Query: 392 IPCIGEKLDERESNKTNEV 448
+ CIGEKLDERE+ T V
Sbjct: 123 VACIGEKLDEREAGITEGV 141
>sp|P00939|TPIS_RABIT Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 248
Score = 192 bits (487), Expect = 4e-49
Identities = 93/139 (66%), Positives = 106/139 (76%)
Frame = +2
Query: 32 RKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKIGVSA 211
RKFFVGGNWKMNG K L ++ +L AK+ TEVV APP+ Y+DF RQKL KI V+A
Sbjct: 4 RKFFVGGNWKMNGRKKNLGELITTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAA 63
Query: 212 QNCYKIPKGAFTGEISPAMIKDNMCDWVILGHSERRHVFGESDHLIGEKVKHALESGLSI 391
QNCYK+ GAFTGEISP MIKD WV+LGHSERRHVFGESD LIG+KV HAL GL +
Sbjct: 64 QNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALSEGLGV 123
Query: 392 IPCIGEKLDERESNKTNEV 448
I CIGEKLDERE+ T +V
Sbjct: 124 IACIGEKLDEREAGITEKV 142
>sp|P48500|TPIS_RAT Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 249
Score = 192 bits (487), Expect = 4e-49
Identities = 94/143 (65%), Positives = 108/143 (75%)
Frame = +2
Query: 20 MSNQRKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKI 199
M+ RKFFVGGNWKMNG K L ++ +L AKL TEVV APP+ Y+DF RQKL KI
Sbjct: 1 MAPSRKFFVGGNWKMNGRKKCLGELICTLNAAKLPADTEVVCAPPTAYIDFARQKLDPKI 60
Query: 200 GVSAQNCYKIPKGAFTGEISPAMIKDNMCDWVILGHSERRHVFGESDHLIGEKVKHALES 379
V+AQNCYK+ GAFTGEISP MIKD WV+LGHSERRH+FGESD LIG+KV HAL
Sbjct: 61 AVAAQNCYKVTNGAFTGEISPGMIKDLGATWVVLGHSERRHIFGESDELIGQKVNHALSE 120
Query: 380 GLSIIPCIGEKLDERESNKTNEV 448
GL +I CIGEKLDERE+ T +V
Sbjct: 121 GLEVIACIGEKLDEREAGITEKV 143
>sp|P17751|TPIS_MOUSE Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 249
Score = 188 bits (477), Expect = 5e-48
Identities = 93/143 (65%), Positives = 107/143 (74%)
Frame = +2
Query: 20 MSNQRKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKI 199
M+ RKFFVGGNWKMNG K L ++ +L A + TEVV APP+ Y+DF RQKL KI
Sbjct: 1 MAPTRKFFVGGNWKMNGRKKCLGELICTLNAANVPAGTEVVCAPPTAYIDFARQKLDPKI 60
Query: 200 GVSAQNCYKIPKGAFTGEISPAMIKDNMCDWVILGHSERRHVFGESDHLIGEKVKHALES 379
V+AQNCYK+ GAFTGEISP MIKD WV+LGHSERRHVFGESD LIG+KV HAL
Sbjct: 61 AVAAQNCYKVTNGAFTGEISPGMIKDLGATWVVLGHSERRHVFGESDELIGQKVSHALAE 120
Query: 380 GLSIIPCIGEKLDERESNKTNEV 448
GL +I CIGEKLDERE+ T +V
Sbjct: 121 GLGVIACIGEKLDEREAGITEKV 143
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 48,841,351
Number of Sequences: 369166
Number of extensions: 919826
Number of successful extensions: 2674
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2486
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2526
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2394311040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)