Planarian EST Database


Dr_sW_022_M12

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_022_M12
         (542 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9PJM5|RL24_CHLMU  50S ribosomal protein L24                    34   0.20 
sp|P28537|RL24_CHLTR  50S ribosomal protein L24                    32   1.00 
sp|O75592|MYCB2_HUMAN  Probable ubiquitin ligase protein MYC...    32   1.00 
sp|Q7TPH6|MYCB2_MOUSE  Probable ubiquitin ligase protein MYC...    32   1.3  
sp|Q6B2C0|MTAL2_YEAST  Mating-type protein ALPHA2 (MATalpha2...    31   2.2  
sp|Q7NB79|MRAW_MYCGA  S-adenosyl-methyltransferase mraW            31   2.2  
sp|P48602|VATA1_DROME  Vacuolar ATP synthase catalytic subun...    30   2.9  
sp|P20646|PPAP_RAT  Prostatic acid phosphatase precursor           30   2.9  
sp|P46023|GR101_LYMST  G-protein coupled receptor GRL101 pre...    30   4.9  
sp|P49916|DNL3_HUMAN  DNA ligase III (Polydeoxyribonucleotid...    29   6.5  
>sp|Q9PJM5|RL24_CHLMU 50S ribosomal protein L24
          Length = 111

 Score = 34.3 bits (77), Expect = 0.20
 Identities = 21/86 (24%), Positives = 45/86 (52%), Gaps = 2/86 (2%)
 Frame = +2

Query: 50  LCLGNTIVNVAKNKPVIYGSIKDGIKETLANDGIID--KNCFMSKDIGEKTWEVNLLYSI 223
           +C+G+T+  +A N     G +   +K+ +  +GI    KN   S++   K   +N+   +
Sbjct: 6   VCVGDTVYVLAGNDKGKQGKVLRCLKDKVVVEGINVRVKNIKRSQE-NPKGKRINIEAPL 64

Query: 224 HVTELELYSDNDPSELRISITSQTKK 301
           H++ + L  DN P+ L + +T + ++
Sbjct: 65  HISNVRLSIDNQPARLFVKVTEKGRE 90
>sp|P28537|RL24_CHLTR 50S ribosomal protein L24
          Length = 111

 Score = 32.0 bits (71), Expect = 1.00
 Identities = 20/86 (23%), Positives = 44/86 (51%), Gaps = 2/86 (2%)
 Frame = +2

Query: 50  LCLGNTIVNVAKNKPVIYGSIKDGIKETLANDGIID--KNCFMSKDIGEKTWEVNLLYSI 223
           +C+G+T+  +A N     G +   +K+ +  +GI    KN   S++   K   +N+   +
Sbjct: 6   VCVGDTVYVLAGNDKGKQGKVLRCLKDKVVVEGINVRVKNIKRSQE-NPKGKRINIEAPL 64

Query: 224 HVTELELYSDNDPSELRISITSQTKK 301
           H++ + L  DN P+ L + +  + ++
Sbjct: 65  HISNVRLSIDNQPARLFVKVREKGRE 90
>sp|O75592|MYCB2_HUMAN Probable ubiquitin ligase protein MYCBP2 (Myc binding protein 2)
            (Protein associated with Myc) (Pam/highwire/rpm-1
            protein)
          Length = 4641

 Score = 32.0 bits (71), Expect = 1.00
 Identities = 28/105 (26%), Positives = 48/105 (45%), Gaps = 7/105 (6%)
 Frame = +2

Query: 2    NFMILLVTGLLPILVELCLG------NTIVNV-AKNKPVIYGSIKDGIKETLANDGIIDK 160
            +F I + +G   +  ELC+        +IV++   ++P + GS+ DG  ET    G  DK
Sbjct: 3680 SFSISIQSGFEAMSQELCIVMCLKDLTSIVDIKTSSRPAMIGSLTDGSTETFWESGDEDK 3739

Query: 161  NCFMSKDIGEKTWEVNLLYSIHVTELELYSDNDPSELRISITSQT 295
            N         K   +N +  I+   + ++ DN   +L   +TS T
Sbjct: 3740 N-------KTKNITINCVKGINARYVSVHVDNS-RDLGNKVTSMT 3776
>sp|Q7TPH6|MYCB2_MOUSE Probable ubiquitin ligase protein MYCBP2 (Myc binding protein 2)
            (Protein associated with Myc) (Pam/highwire/rpm-1
            protein)
          Length = 4711

 Score = 31.6 bits (70), Expect = 1.3
 Identities = 28/105 (26%), Positives = 48/105 (45%), Gaps = 7/105 (6%)
 Frame = +2

Query: 2    NFMILLVTGLLPILVELCLG------NTIVNV-AKNKPVIYGSIKDGIKETLANDGIIDK 160
            +F I + +G   +  ELC+        +IV++   ++P + GS+ DG  ET    G  DK
Sbjct: 3750 SFSISVQSGFEAMSQELCIVMCLKDLTSIVDIKTSSRPAMIGSLTDGSTETFWESGDEDK 3809

Query: 161  NCFMSKDIGEKTWEVNLLYSIHVTELELYSDNDPSELRISITSQT 295
            N         K   +N +  I+   + ++ DN   +L   +TS T
Sbjct: 3810 N-------KTKNITINCVKGINARYVSVHVDNS-RDLGNKVTSMT 3846
>sp|Q6B2C0|MTAL2_YEAST Mating-type protein ALPHA2 (MATalpha2 protein) (Alpha-2 repressor)
          Length = 210

 Score = 30.8 bits (68), Expect = 2.2
 Identities = 21/56 (37%), Positives = 31/56 (55%), Gaps = 3/56 (5%)
 Frame = -3

Query: 282 IEIRNSLGSLSEYNSNSVTWIEYSKL---TSQVFSPISLLIKQFLSIIPSFANVSL 124
           +E+R+ LG LS  N N     E  KL   TSQ+ + I++L+K+  SI    +N  L
Sbjct: 49  VELRDILGFLSRANKNRKISDEEKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQL 104
>sp|Q7NB79|MRAW_MYCGA S-adenosyl-methyltransferase mraW
          Length = 317

 Score = 30.8 bits (68), Expect = 2.2
 Identities = 26/89 (29%), Positives = 43/89 (48%), Gaps = 5/89 (5%)
 Frame = +2

Query: 41  LVELCLGNTIVNVAKNKPVIYGSIKDGIKETLANDGIIDKNCFMSKDIGEKTWEVNLLYS 220
           L++    + +V + KN    YG IKD  +  +A      K  F  K++   T EV  L  
Sbjct: 146 LIQNSTVSELVKIMKN----YGEIKDPYRVVVAL-----KKAFEKKELN--TLEVVELIK 194

Query: 221 IHVTELELYSDNDPSE-----LRISITSQ 292
            HV + ELY++  P+      LRI++ ++
Sbjct: 195 KHVNKAELYANKHPARRYFQALRIAVNNE 223
>sp|P48602|VATA1_DROME Vacuolar ATP synthase catalytic subunit A isoform 1 (V-ATPase A
           subunit 1) (Vacuolar proton pump alpha subunit 1)
           (V-ATPase 69 kDa subunit 1)
          Length = 614

 Score = 30.4 bits (67), Expect = 2.9
 Identities = 22/83 (26%), Positives = 40/83 (48%), Gaps = 12/83 (14%)
 Frame = +2

Query: 92  PVIYGSIKDGIKETLANDGIIDKNCFMSKDIG------EKTWE---VNLLYSIHVTELEL 244
           P I GSI DGI+  L + G++  + ++ K +        + WE   +N+    H+T  +L
Sbjct: 90  PGIMGSIFDGIQRPLRDIGVMTNSIYIPKGVNTTALSRSEMWEFNPLNVRVGSHITGGDL 149

Query: 245 YS---DNDPSELRISITSQTKKT 304
           Y    +N   + R+ +  + K T
Sbjct: 150 YGVVHENTLVKQRMIVAPRAKGT 172
>sp|P20646|PPAP_RAT Prostatic acid phosphatase precursor
          Length = 381

 Score = 30.4 bits (67), Expect = 2.9
 Identities = 15/51 (29%), Positives = 28/51 (54%)
 Frame = +2

Query: 218 SIHVTELELYSDNDPSELRISITSQTKKTKFPVAAKSCANGCKIPIKKDAE 370
           S H+  +ELY DN  + + +   ++T+   +P+    C + C  P++K AE
Sbjct: 311 SCHI--MELYQDNGGTFVEMYYRNETQNEPYPLTLPGCTHSC--PLEKFAE 357
>sp|P46023|GR101_LYMST G-protein coupled receptor GRL101 precursor
          Length = 1115

 Score = 29.6 bits (65), Expect = 4.9
 Identities = 16/45 (35%), Positives = 28/45 (62%)
 Frame = +2

Query: 65  TIVNVAKNKPVIYGSIKDGIKETLANDGIIDKNCFMSKDIGEKTW 199
           T++N+++N+     SI +G    LAN  +ID +  + KDIG+K +
Sbjct: 682 TVLNISRNQ---INSIDNGAFNNLANVRLIDLSGNVIKDIGQKVF 723
>sp|P49916|DNL3_HUMAN DNA ligase III (Polydeoxyribonucleotide synthase [ATP])
          Length = 922

 Score = 29.3 bits (64), Expect = 6.5
 Identities = 13/26 (50%), Positives = 15/26 (57%)
 Frame = +2

Query: 317 AAKSCANGCKIPIKKDAEKVQIEKTG 394
           A K C NG    IK D E+VQ+ K G
Sbjct: 408 AMKKCPNGMFSEIKYDGERVQVHKNG 433
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 50,928,270
Number of Sequences: 369166
Number of extensions: 874509
Number of successful extensions: 2608
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2540
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2605
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 3734833040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)