Planarian EST Database


Dr_sW_022_M05

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_022_M05
         (389 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9BW30|CG38_HUMAN  Protein CGI-38                               57   1e-08
sp|Q9CRB6|CG38_MOUSE  Protein CGI-38 homolog                       55   4e-08
sp|Q7TQD2|P25A_MOUSE  Tubulin polymerization-promoting prote...    52   4e-07
sp|Q27957|P25A_BOVIN  Tubulin polymerization-promoting prote...    51   9e-07
sp|O94811|P25A_HUMAN  Tubulin polymerization-promoting prote...    50   1e-06
sp|P59282|P25B_HUMAN  Protein p25-beta                             48   8e-06
sp|Q9VV43|Y4893_DROME  Hypothetical protein CG4893                 43   3e-04
sp|P91127|YBYK_CAEEL  Hypothetical protein C32E8.3 in chromo...    41   7e-04
sp|Q4L5U2|TRMD_STAHJ  tRNA (Guanine-N(1)-)-methyltransferase...    33   0.26 
sp|P16342|R1AB_CVMA5  Replicase polyprotein 1ab (pp1ab) (ORF...    32   0.58 
>sp|Q9BW30|CG38_HUMAN Protein CGI-38
          Length = 176

 Score = 57.4 bits (137), Expect = 1e-08
 Identities = 36/111 (32%), Positives = 51/111 (45%), Gaps = 1/111 (0%)
 Frame = +3

Query: 54  KTLKKICTDCKIYC-KGFDQNRCDIQFRKHIGNAKKDVDYNDFVRFVEGPMAEAYMSARN 230
           K   K+C DCK+   K       DI F K  G + + ++Y +F + +E    + +     
Sbjct: 33  KNWAKLCKDCKVADGKSVTGTDVDIVFSKVKGKSARVINYEEFKKALEELATKRFKGKSK 92

Query: 231 LAYEDAVQELKTKICQGSPAGHGTTGVSADAATARLTDVKGYTGAHKERFD 383
               DA+ +L   +    PA  G T      A  RLTD   YTG+HKERFD
Sbjct: 93  EEAFDAICQL---VAGKEPANVGVTKAKTGGAVDRLTDTSRYTGSHKERFD 140
>sp|Q9CRB6|CG38_MOUSE Protein CGI-38 homolog
          Length = 176

 Score = 55.5 bits (132), Expect = 4e-08
 Identities = 36/111 (32%), Positives = 50/111 (45%), Gaps = 1/111 (0%)
 Frame = +3

Query: 54  KTLKKICTDCKIYC-KGFDQNRCDIQFRKHIGNAKKDVDYNDFVRFVEGPMAEAYMSARN 230
           K   K+C DCK+   K       DI F K    + + ++Y +F + +E    + +     
Sbjct: 33  KNWAKLCKDCKVADGKAVTGTDVDIVFSKVKAKSARVINYEEFKKALEELATKRFKGKSK 92

Query: 231 LAYEDAVQELKTKICQGSPAGHGTTGVSADAATARLTDVKGYTGAHKERFD 383
               DA+ +L   I    PA  G T      A  RLTD   YTG+HKERFD
Sbjct: 93  EEAFDAICQL---IAGKEPANIGVTKAKTGGAVDRLTDTSKYTGSHKERFD 140
>sp|Q7TQD2|P25A_MOUSE Tubulin polymerization-promoting protein (TPPP)
          Length = 218

 Score = 52.0 bits (123), Expect = 4e-07
 Identities = 36/123 (29%), Positives = 57/123 (46%), Gaps = 6/123 (4%)
 Frame = +3

Query: 33  GSTTCTDKTL-----KKICTDCKIYC-KGFDQNRCDIQFRKHIGNAKKDVDYNDFVRFVE 194
           G T  T K +      K+C DC +   K       DI F K  G + + + +  F   +E
Sbjct: 61  GDTRATGKEMHGKNWSKLCKDCHVIDGKNVTVTDVDIVFSKIKGKSCRTITFEQFQEALE 120

Query: 195 GPMAEAYMSARNLAYEDAVQELKTKICQGSPAGHGTTGVSADAATARLTDVKGYTGAHKE 374
               + +   ++ + E+AV+E+   I   +P   G T   +    +RLTD   +TG+HKE
Sbjct: 121 ELAKKRF---KDKSSEEAVREVHRLIEGRAPVISGVTKAVSSPTVSRLTDTSKFTGSHKE 177

Query: 375 RFD 383
           RFD
Sbjct: 178 RFD 180
>sp|Q27957|P25A_BOVIN Tubulin polymerization-promoting protein (TPPP) (25 kDa
           brain-specific protein) (p25-alpha)
          Length = 218

 Score = 50.8 bits (120), Expect = 9e-07
 Identities = 32/111 (28%), Positives = 54/111 (48%), Gaps = 1/111 (0%)
 Frame = +3

Query: 54  KTLKKICTDCKIYC-KGFDQNRCDIQFRKHIGNAKKDVDYNDFVRFVEGPMAEAYMSARN 230
           K   K+C DC++   +       DI F K  G + + + +  F   +E    + +   ++
Sbjct: 73  KNWSKLCRDCQVIDGRSVTVTDVDIVFSKIKGKSCRTITFEQFKEALEELAKKRF---KD 129

Query: 231 LAYEDAVQELKTKICQGSPAGHGTTGVSADAATARLTDVKGYTGAHKERFD 383
            + E+AV+E+   I   +P   G T   +    +RLTD   +TG+HKERFD
Sbjct: 130 KSAEEAVREVHKLIEGKAPIISGVTKAISSPTVSRLTDTSKFTGSHKERFD 180
>sp|O94811|P25A_HUMAN Tubulin polymerization-promoting protein (TPPP) (25 kDa
           brain-specific protein) (p25-alpha) (p24) (p25)
          Length = 219

 Score = 50.4 bits (119), Expect = 1e-06
 Identities = 32/111 (28%), Positives = 54/111 (48%), Gaps = 1/111 (0%)
 Frame = +3

Query: 54  KTLKKICTDCKIYC-KGFDQNRCDIQFRKHIGNAKKDVDYNDFVRFVEGPMAEAYMSARN 230
           K   K+C DC++   +       DI F K  G + + + +  F   +E    + +   ++
Sbjct: 74  KNWSKLCKDCQVIDGRNVTVTDVDIVFSKIKGKSCRTITFEQFQEALEELAKKRF---KD 130

Query: 231 LAYEDAVQELKTKICQGSPAGHGTTGVSADAATARLTDVKGYTGAHKERFD 383
            + E+AV+E+   I   +P   G T   +    +RLTD   +TG+HKERFD
Sbjct: 131 KSSEEAVREVHRLIEGKAPIISGVTKAISSPTVSRLTDTTKFTGSHKERFD 181
>sp|P59282|P25B_HUMAN Protein p25-beta
          Length = 170

 Score = 47.8 bits (112), Expect = 8e-06
 Identities = 40/129 (31%), Positives = 55/129 (42%), Gaps = 2/129 (1%)
 Frame = +3

Query: 3   FAAFCDAVKKGSTTCTDKTLKKICTDCKIYC-KGFDQNRCDIQFRKHIGNAKKDVDYNDF 179
           FAAF ++   G T   +K   K+C DC I   K       DI F K      + + +  F
Sbjct: 12  FAAFGESSSSG-TEMNNKNFSKLCKDCGIMDGKTVTSTDVDIVFSKVKAKNARTITFQQF 70

Query: 180 VRFVEGPMAEAYMSARNLAYEDAVQELKTKICQGS-PAGHGTTGVSADAATARLTDVKGY 356
              V+  + +     ++    D V E    + +G  PA  G T  +   A  RLTD   Y
Sbjct: 71  KEAVK-ELGQKRFKGKS---PDEVLENIYGLMEGKDPATTGATKATTVGAVDRLTDTSKY 126

Query: 357 TGAHKERFD 383
           TG HKE FD
Sbjct: 127 TGTHKELFD 135
>sp|Q9VV43|Y4893_DROME Hypothetical protein CG4893
          Length = 192

 Score = 42.7 bits (99), Expect = 3e-04
 Identities = 40/132 (30%), Positives = 58/132 (43%), Gaps = 4/132 (3%)
 Frame = +3

Query: 3   FAAFCDAVKKG---STTCTDKTLKKICTDCKIYCKGFDQNRCDIQFRKHIGNAKKDVDYN 173
           F+ F D+   G   + + +DK +K+     K+  K        I F+K         DYN
Sbjct: 44  FSKFGDSKSDGKLITLSQSDKWMKQ----AKVIDKKITTTDTGIHFKKFKAMKISLSDYN 99

Query: 174 DFVRFVEGPMAEAYMSARNLAYEDAVQELKTKICQ-GSPAGHGTTGVSADAATARLTDVK 350
            F+  +          A+    E  + E+K K+   G+P     +   A AA  RLTD  
Sbjct: 100 KFLDDL----------AKTKKVE--LSEIKQKLASCGAPGVVSVSAGKAAAAVDRLTDTS 147

Query: 351 GYTGAHKERFDA 386
            YTG+HKERFDA
Sbjct: 148 KYTGSHKERFDA 159
>sp|P91127|YBYK_CAEEL Hypothetical protein C32E8.3 in chromosome I
          Length = 180

 Score = 41.2 bits (95), Expect = 7e-04
 Identities = 23/49 (46%), Positives = 29/49 (59%)
 Frame = +3

Query: 243 DAVQELKTKICQGSPAGHGTTGVSADAATARLTDVKGYTGAHKERFDAE 389
           DA+ E   K+   +P+  G    +A    +RLTD   YTGAHKERFDAE
Sbjct: 100 DAITEKLAKL--EAPSVGGAAKANAAGVYSRLTDHTKYTGAHKERFDAE 146
>sp|Q4L5U2|TRMD_STAHJ tRNA (Guanine-N(1)-)-methyltransferase (M1G-methyltransferase)
           (tRNA [GM37] methyltransferase)
          Length = 245

 Score = 32.7 bits (73), Expect = 0.26
 Identities = 21/48 (43%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
 Frame = -3

Query: 345 HLSNEQLPHQQKL-RLYHGRQDYLDRF*FSILVLRLHKPNFLHSYKLQ 205
           H + EQ  H+QKL R YH R D LDR+      L      F+ SYK Q
Sbjct: 199 HANIEQWRHEQKLIRTYHKRPDLLDRY-----PLTEDDEKFIESYKKQ 241
>sp|P16342|R1AB_CVMA5 Replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) [Includes:
            Replicase polyprotein 1a (pp1a) (ORF1a)] [Contains: p28;
            p65; p210 (Papain-like proteinases 1/2)
            (PL1-PRO/PL2-PRO); Peptide HD2 (p44); 3C-like proteinase
            (3CL-PRO) (3CLp) (M-PRO) (p27); Unknown protein 1; p10;
            p22; p12; Growth factor-like peptide (GFL) (p15);
            RNA-directed RNA polymerase (RdRp) (Pol) (p100); Helicase
            (Hel) (p67); Unknown protein 2; p35; Unknown protein 3]
          Length = 7176

 Score = 31.6 bits (70), Expect = 0.58
 Identities = 13/45 (28%), Positives = 25/45 (55%)
 Frame = -1

Query: 326  CRISRNSGCTMAGRTTLTDFSFQFLYCVFISQISCTHISFSHWSF 192
            CR++ +  C M+GR +LT  S+Q   C  +  ++  + +   +SF
Sbjct: 3392 CRVTSSDFCVMSGRMSLTVMSYQMQGCQLVLTVTLQNPNTPKYSF 3436
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,910,422
Number of Sequences: 369166
Number of extensions: 809979
Number of successful extensions: 2932
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2899
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2931
length of database: 68,354,980
effective HSP length: 96
effective length of database: 50,620,420
effective search space used: 1670473860
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)