Planarian EST Database


Dr_sW_022_M03

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_022_M03
         (457 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P47830|RL27A_XENLA  60S ribosomal protein L27a (L22)           191   5e-49
sp|P18445|RL27A_RAT  60S ribosomal protein L27a                   187   9e-48
sp|Q5REY2|RL27A_PONPY  60S ribosomal protein L27a >gi|117301...   187   1e-47
sp|Q5R1X0|RL27A_PANTR  60S ribosomal protein L27a                 185   4e-47
sp|P14115|RL27A_MOUSE  60S ribosomal protein L27a (L29)           185   5e-47
sp|O01358|RL27A_OSCBR  60S ribosomal protein L27a (Ribosomal...   172   4e-43
sp|P41092|RL27A_DROME  60S ribosomal protein L27a                 159   4e-39
sp|P48160|RL27A_DICDI  60S ribosomal protein L27a                 157   1e-38
sp|Q27021|RL27A_TENMO  60S ribosomal protein L27a                 157   1e-38
sp|Q9BI14|R27A3_ENTHI  60S ribosomal protein L27a-3               153   2e-37
>sp|P47830|RL27A_XENLA 60S ribosomal protein L27a (L22)
          Length = 148

 Score =  191 bits (486), Expect = 5e-49
 Identities = 91/143 (63%), Positives = 107/143 (74%)
 Frame = +1

Query: 4   KKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRK 183
           +KTRKL                      NAGG HHHRI+FDK+HPGYFGKVGMRHYHL+K
Sbjct: 6   RKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHYHLKK 65

Query: 184 NRTFCPSINIDKIWSLVSEKTYETYKDNAEGKAPVIDCLKAGYFKVLGNGVLPKVPVIVK 363
           N++FCP+IN+DK+W+LVSE+T   +  N EG AP+ID + AGY+KVLG G LPK PVIVK
Sbjct: 66  NQSFCPTINLDKLWTLVSEQTRLNHAKNLEGPAPIIDAVHAGYYKVLGKGKLPKQPVIVK 125

Query: 364 AKFFSRQAEEKIKSVGGACVLVA 432
           AKFFSR+AEEKIKSVGGACVL A
Sbjct: 126 AKFFSRKAEEKIKSVGGACVLGA 148
>sp|P18445|RL27A_RAT 60S ribosomal protein L27a
          Length = 148

 Score =  187 bits (475), Expect = 9e-48
 Identities = 87/143 (60%), Positives = 106/143 (74%)
 Frame = +1

Query: 4   KKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRK 183
           +KTRKL                      NAGG HHHRI+FDK+HPGYFGKVGMRHYHL++
Sbjct: 6   RKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHYHLKR 65

Query: 184 NRTFCPSINIDKIWSLVSEKTYETYKDNAEGKAPVIDCLKAGYFKVLGNGVLPKVPVIVK 363
           N++FCP++N+DK+W+LVSE+T      N  G AP+ID +++GY+KVLG G LPK PVIVK
Sbjct: 66  NQSFCPTVNLDKLWTLVSEQTRVNAAKNKNGVAPIIDVVRSGYYKVLGKGKLPKQPVIVK 125

Query: 364 AKFFSRQAEEKIKSVGGACVLVA 432
           AKFFSR+AEEKIK VGGACVLVA
Sbjct: 126 AKFFSRRAEEKIKGVGGACVLVA 148
>sp|Q5REY2|RL27A_PONPY 60S ribosomal protein L27a
 sp|P46776|RL27A_HUMAN 60S ribosomal protein L27a
          Length = 148

 Score =  187 bits (474), Expect = 1e-47
 Identities = 87/143 (60%), Positives = 107/143 (74%)
 Frame = +1

Query: 4   KKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRK 183
           +KTRKL                      NAGG HHHRI+FDK+HPGYFGKVGM+HYHL++
Sbjct: 6   RKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGLHHHRINFDKYHPGYFGKVGMKHYHLKR 65

Query: 184 NRTFCPSINIDKIWSLVSEKTYETYKDNAEGKAPVIDCLKAGYFKVLGNGVLPKVPVIVK 363
           N++FCP++N+DK+W+LVSE+T      N  G AP+ID +++GY+KVLG G LPK PVIVK
Sbjct: 66  NQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVK 125

Query: 364 AKFFSRQAEEKIKSVGGACVLVA 432
           AKFFSR+AEEKIKSVGGACVLVA
Sbjct: 126 AKFFSRRAEEKIKSVGGACVLVA 148
>sp|Q5R1X0|RL27A_PANTR 60S ribosomal protein L27a
          Length = 148

 Score =  185 bits (470), Expect = 4e-47
 Identities = 86/142 (60%), Positives = 106/142 (74%)
 Frame = +1

Query: 4   KKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRK 183
           +KTRKL                      NAGG HHHRI+FDK+HPGYFGKVGM+HYHL++
Sbjct: 6   RKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGLHHHRINFDKYHPGYFGKVGMKHYHLKR 65

Query: 184 NRTFCPSINIDKIWSLVSEKTYETYKDNAEGKAPVIDCLKAGYFKVLGNGVLPKVPVIVK 363
           N++FCP++N+DK+W+LVSE+T      N  G AP+ID +++GY+KVLG G LPK PVIVK
Sbjct: 66  NQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVK 125

Query: 364 AKFFSRQAEEKIKSVGGACVLV 429
           AKFFSR+AEEKIKSVGGACVLV
Sbjct: 126 AKFFSRRAEEKIKSVGGACVLV 147
>sp|P14115|RL27A_MOUSE 60S ribosomal protein L27a (L29)
          Length = 148

 Score =  185 bits (469), Expect = 5e-47
 Identities = 87/143 (60%), Positives = 105/143 (73%)
 Frame = +1

Query: 4   KKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRK 183
           +KTRKL                      NAGG HHHRI+FDK+HPGYFGKVGMRHYHL++
Sbjct: 6   RKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHYHLKR 65

Query: 184 NRTFCPSINIDKIWSLVSEKTYETYKDNAEGKAPVIDCLKAGYFKVLGNGVLPKVPVIVK 363
           N++FCP++N+DK W+LVSE+T      N  G AP+ID +++GY+KVLG G LPK PVIVK
Sbjct: 66  NQSFCPTVNLDKPWTLVSEQTRVNAAKNKTGVAPIIDVVRSGYYKVLGKGKLPKQPVIVK 125

Query: 364 AKFFSRQAEEKIKSVGGACVLVA 432
           AKFFSR+AEEKIK VGGACVLVA
Sbjct: 126 AKFFSRRAEEKIKGVGGACVLVA 148
>sp|O01358|RL27A_OSCBR 60S ribosomal protein L27a (Ribosomal protein RPL-27)
          Length = 145

 Score =  172 bits (435), Expect = 4e-43
 Identities = 84/143 (58%), Positives = 104/143 (72%)
 Frame = +1

Query: 4   KKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRK 183
           +KTRKL                      NAGGQHHHRI+ DK+HPGYFGKVGMR +HL K
Sbjct: 6   RKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGQHHHRINRDKYHPGYFGKVGMRVFHLNK 65

Query: 184 NRTFCPSINIDKIWSLVSEKTYETYKDNAEGKAPVIDCLKAGYFKVLGNGVLPKVPVIVK 363
           N  +CP++N+D++W+LV E+  +  K +AE KAPVIDC+KAGYFKVLG G+LPK P+IVK
Sbjct: 66  NHHYCPTVNVDRLWALVPEE--QKTKVSAE-KAPVIDCVKAGYFKVLGKGLLPKQPLIVK 122

Query: 364 AKFFSRQAEEKIKSVGGACVLVA 432
           AKFFS +AE KIK+ GGAC+LVA
Sbjct: 123 AKFFSHEAENKIKAAGGACILVA 145
>sp|P41092|RL27A_DROME 60S ribosomal protein L27a
          Length = 149

 Score =  159 bits (401), Expect = 4e-39
 Identities = 76/143 (53%), Positives = 94/143 (65%)
 Frame = +1

Query: 4   KKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRK 183
           KKTRKL                      NAGG HHHRI+FDK+HPGYFGKVGMR++HLR+
Sbjct: 7   KKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRNFHLRR 66

Query: 184 NRTFCPSINIDKIWSLVSEKTYETYKDNAEGKAPVIDCLKAGYFKVLGNGVLPKVPVIVK 363
              F P IN+DK+WSLV  + +   +     KAPVID +K GY+K+LG G LP  PVIVK
Sbjct: 67  QHKFRPEINLDKLWSLVGAEKFAELEKEKSTKAPVIDLVKFGYYKLLGRGHLPARPVIVK 126

Query: 364 AKFFSRQAEEKIKSVGGACVLVA 432
           AK+FS++AE+KIK  GG C+L A
Sbjct: 127 AKYFSKKAEDKIKKAGGVCLLSA 149
>sp|P48160|RL27A_DICDI 60S ribosomal protein L27a
          Length = 148

 Score =  157 bits (397), Expect = 1e-38
 Identities = 67/115 (58%), Positives = 94/115 (81%)
 Frame = +1

Query: 88  NAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSINIDKIWSLVSEKTYETYKDN 267
           NAGG  HHRI+FDK+HPGYFGK+GMRH+HL +N+  CP+++++KIW+LV E   ++    
Sbjct: 34  NAGGLTHHRINFDKYHPGYFGKLGMRHFHLLRNQYHCPTVSLEKIWTLVPESVRKSLAAK 93

Query: 268 AEGKAPVIDCLKAGYFKVLGNGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 432
            +G APV+D  + G+FKVLG+G+LP  P+IVKA++FS+ AE+KIK+VGGAC+LVA
Sbjct: 94  NDGTAPVVDVTQKGFFKVLGHGILPTQPIIVKARYFSKVAEKKIKAVGGACILVA 148
>sp|Q27021|RL27A_TENMO 60S ribosomal protein L27a
          Length = 148

 Score =  157 bits (396), Expect = 1e-38
 Identities = 75/143 (52%), Positives = 97/143 (67%)
 Frame = +1

Query: 4   KKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRK 183
           KKTRKL                      NAGG HHHRI+FDK+HPGYFGK+GMR+YHLR+
Sbjct: 6   KKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKLGMRNYHLRR 65

Query: 184 NRTFCPSINIDKIWSLVSEKTYETYKDNAEGKAPVIDCLKAGYFKVLGNGVLPKVPVIVK 363
           N  + P+IN+DK+W+LVSE+T   Y+++ EGKAPVID +KAGY+K+LG G       ++K
Sbjct: 66  NSKWAPAINLDKLWTLVSEQTRSKYQNHPEGKAPVIDIVKAGYYKLLGKGACLTNLSLLK 125

Query: 364 AKFFSRQAEEKIKSVGGACVLVA 432
             F   + E+KIK+VGGACVL A
Sbjct: 126 PSFSLNRLEDKIKAVGGACVLSA 148
>sp|Q9BI14|R27A3_ENTHI 60S ribosomal protein L27a-3
          Length = 149

 Score =  153 bits (386), Expect = 2e-37
 Identities = 70/116 (60%), Positives = 88/116 (75%), Gaps = 1/116 (0%)
 Frame = +1

Query: 88  NAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSINIDKIWSLVSEKTYETYKDN 267
           NAGGQHH +  F  FHP YFGK GMR +HL+ N+ +CPSIN+D +WSLV +   E YK+ 
Sbjct: 34  NAGGQHHRKTWFTTFHPDYFGKHGMRVFHLKANKYYCPSINVDSLWSLVGKDVQEQYKNA 93

Query: 268 AEG-KAPVIDCLKAGYFKVLGNGVLPKVPVIVKAKFFSRQAEEKIKSVGGACVLVA 432
             G + PVIDC+K GYFKVLG G LPK PVIV+A++FS +A++KIK+VGGAC L A
Sbjct: 94  KTGEEVPVIDCVKHGYFKVLGKGFLPKQPVIVRARYFSEKAQQKIKAVGGACELTA 149
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 46,435,430
Number of Sequences: 369166
Number of extensions: 831433
Number of successful extensions: 2293
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2192
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2253
length of database: 68,354,980
effective HSP length: 101
effective length of database: 49,696,745
effective search space used: 2484837250
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)