Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_022_L19
(621 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P46467|VPS4B_MOUSE Vacuolar sorting protein 4b (SKD1 pro... 236 3e-62
sp|O75351|VPS4B_HUMAN Vacuolar sorting protein 4b (SKD1 pro... 234 1e-61
sp|Q8VEJ9|VPS4A_MOUSE Vacuolar sorting protein 4a 226 4e-59
sp|Q9UN37|VPS4A_HUMAN Vacuolar sorting protein 4a (SKD2 pro... 224 2e-58
sp|Q09803|VPS4_SCHPO Suppressor protein of bem1/bed5 double... 182 8e-46
sp|P52917|VPS4_YEAST Vacuolar protein sorting-associated pr... 178 1e-44
sp|Q5XIK7|KATL1_RAT Katanin p60 ATPase-containing subunit A... 92 1e-18
sp|Q8K0T4|KATL1_MOUSE Katanin p60 ATPase-containing subunit... 91 2e-18
sp|Q9BW62|KATL1_HUMAN Katanin p60 ATPase-containing subunit... 89 9e-18
sp|P39955|SAP1_YEAST SAP1 protein 86 8e-17
>sp|P46467|VPS4B_MOUSE Vacuolar sorting protein 4b (SKD1 protein)
Length = 444
Score = 236 bits (603), Expect = 3e-62
Identities = 119/177 (67%), Positives = 140/177 (79%)
Frame = +1
Query: 19 DNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAGARAEMFKLSMGNKPGQHDLTPQN 198
DND +L+L ATNIPW LDSAIRRRFEKRIYI LPEA ARA MF+L +G+ Q+ LT +
Sbjct: 268 DNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEAHARAAMFRLHLGST--QNSLTEAD 325
Query: 199 FSELGHLSEGYSGADISIAVRDALMVPVRKVQTATHFRRVRGPSPTDPSVIVSDLLTPCS 378
F ELG ++GYSGADISI VRDALM PVRKVQ+ATHF++VRGPS DP+ IV+DLLTPCS
Sbjct: 326 FQELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNCIVNDLLTPCS 385
Query: 379 PGSPGAIEMNWQTVDSEKLLEPCVTFADMKLALSRNKPTVHQKDLEKLIQFTTDFGQ 549
PG PGAIEM W V +KLLEP V+ DM +LS KPTV+++DL KL +FT DFGQ
Sbjct: 386 PGDPGAIEMTWMDVPGDKLLEPVVSMWDMLRSLSSTKPTVNEQDLLKLKKFTEDFGQ 442
>sp|O75351|VPS4B_HUMAN Vacuolar sorting protein 4b (SKD1 protein)
Length = 444
Score = 234 bits (597), Expect = 1e-61
Identities = 118/177 (66%), Positives = 137/177 (77%)
Frame = +1
Query: 19 DNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAGARAEMFKLSMGNKPGQHDLTPQN 198
DND +L+L ATNIPW LDSAIRRRFEKRIYI LPE ARA MFKL +G Q+ LT +
Sbjct: 268 DNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTT--QNSLTEAD 325
Query: 199 FSELGHLSEGYSGADISIAVRDALMVPVRKVQTATHFRRVRGPSPTDPSVIVSDLLTPCS 378
F ELG ++GYSGADISI VRDALM PVRKVQ+ATHF++VRGPS DP+ +V DLLTPCS
Sbjct: 326 FRELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCS 385
Query: 379 PGSPGAIEMNWQTVDSEKLLEPCVTFADMKLALSRNKPTVHQKDLEKLIQFTTDFGQ 549
PG PGAIEM W V +KLLEP V+ +DM +LS KPTV++ DL KL +FT DFGQ
Sbjct: 386 PGDPGAIEMTWMDVPGDKLLEPVVSMSDMLRSLSNTKPTVNEHDLLKLKKFTEDFGQ 442
>sp|Q8VEJ9|VPS4A_MOUSE Vacuolar sorting protein 4a
Length = 437
Score = 226 bits (576), Expect = 4e-59
Identities = 112/177 (63%), Positives = 136/177 (76%)
Frame = +1
Query: 19 DNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAGARAEMFKLSMGNKPGQHDLTPQN 198
+ND L+L ATNIPW LDSAIRRRFEKRIYI LPE ARA+MF+L +G+ P H+LT N
Sbjct: 261 NNDGTLVLGATNIPWVLDSAIRRRFEKRIYIPLPEEAARAQMFRLHLGSTP--HNLTDAN 318
Query: 199 FSELGHLSEGYSGADISIAVRDALMVPVRKVQTATHFRRVRGPSPTDPSVIVSDLLTPCS 378
EL +EGYSGADISI VRD+LM PVRKVQ+ATHF++V GPS T+PSV++ DLLTPCS
Sbjct: 319 IHELARKTEGYSGADISIIVRDSLMQPVRKVQSATHFKKVCGPSRTNPSVMIDDLLTPCS 378
Query: 379 PGSPGAIEMNWQTVDSEKLLEPCVTFADMKLALSRNKPTVHQKDLEKLIQFTTDFGQ 549
PG PGAIEM W V +KLLEP V +DM +L+ +PTV+ DL K+ +F+ DFGQ
Sbjct: 379 PGDPGAIEMTWMDVPGDKLLEPVVCMSDMLRSLATTRPTVNADDLLKVKKFSEDFGQ 435
>sp|Q9UN37|VPS4A_HUMAN Vacuolar sorting protein 4a (SKD2 protein) (hVPS4) (VPS4-1)
Length = 437
Score = 224 bits (570), Expect = 2e-58
Identities = 110/177 (62%), Positives = 136/177 (76%)
Frame = +1
Query: 19 DNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAGARAEMFKLSMGNKPGQHDLTPQN 198
+ND L+L ATNIPW LDSAIRRRFEKRIYI LPE ARA+MF+L +G+ P H+LT N
Sbjct: 261 NNDGTLVLGATNIPWVLDSAIRRRFEKRIYIPLPEEAARAQMFRLHLGSTP--HNLTDAN 318
Query: 199 FSELGHLSEGYSGADISIAVRDALMVPVRKVQTATHFRRVRGPSPTDPSVIVSDLLTPCS 378
EL +EGYSGADISI VRD+LM PVRKVQ+ATHF++V GPS T+PS+++ DLLTPCS
Sbjct: 319 IHELARKTEGYSGADISIIVRDSLMQPVRKVQSATHFKKVCGPSRTNPSMMIDDLLTPCS 378
Query: 379 PGSPGAIEMNWQTVDSEKLLEPCVTFADMKLALSRNKPTVHQKDLEKLIQFTTDFGQ 549
PG PGA+EM W V +KLLEP V +DM +L+ +PTV+ DL K+ +F+ DFGQ
Sbjct: 379 PGDPGAMEMTWMDVPGDKLLEPVVCMSDMLRSLATTRPTVNADDLLKVKKFSEDFGQ 435
>sp|Q09803|VPS4_SCHPO Suppressor protein of bem1/bed5 double mutants
Length = 432
Score = 182 bits (461), Expect = 8e-46
Identities = 94/176 (53%), Positives = 119/176 (67%)
Frame = +1
Query: 19 DNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAGARAEMFKLSMGNKPGQHDLTPQN 198
D VL+L ATNIPW LDSAIRRRFEKRIYI LP A ARA MF+L++G P +LT Q+
Sbjct: 262 DESGVLVLGATNIPWTLDSAIRRRFEKRIYIPLPNAHARARMFELNVGKIPS--ELTSQD 319
Query: 199 FSELGHLSEGYSGADISIAVRDALMVPVRKVQTATHFRRVRGPSPTDPSVIVSDLLTPCS 378
F EL +++GYSG+DISI VRDA+M PVR++ TATHF+ V L+TPCS
Sbjct: 320 FKELAKMTDGYSGSDISIVVRDAIMEPVRRIHTATHFKEVYDNKSN------RTLVTPCS 373
Query: 379 PGSPGAIEMNWQTVDSEKLLEPCVTFADMKLALSRNKPTVHQKDLEKLIQFTTDFG 546
PG P A E +W V+ E ++EP +T D A+ + KPT++ D+EK QFT DFG
Sbjct: 374 PGDPDAFESSWLEVNPEDIMEPKLTVRDFYSAVRKVKPTLNAGDIEKHTQFTKDFG 429
>sp|P52917|VPS4_YEAST Vacuolar protein sorting-associated protein VPS4 (END13 protein)
(DOA4-independent degradation protein 6)
Length = 437
Score = 178 bits (451), Expect = 1e-44
Identities = 91/177 (51%), Positives = 125/177 (70%)
Frame = +1
Query: 19 DNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAGARAEMFKLSMGNKPGQHDLTPQN 198
D+ VL+L ATNIPW LDSAIRRRFE+RIYI LP+ AR MF++++G+ P LT ++
Sbjct: 266 DSQGVLVLGATNIPWQLDSAIRRRFERRIYIPLPDLAARTTMFEINVGDTPCV--LTKED 323
Query: 199 FSELGHLSEGYSGADISIAVRDALMVPVRKVQTATHFRRVRGPSPTDPSVIVSDLLTPCS 378
+ LG ++EGYSG+DI++ V+DALM P+RK+Q+ATHF+ V S D + LTPCS
Sbjct: 324 YRTLGAMTEGYSGSDIAVVVKDALMQPIRKIQSATHFKDV---STEDDE---TRKLTPCS 377
Query: 379 PGSPGAIEMNWQTVDSEKLLEPCVTFADMKLALSRNKPTVHQKDLEKLIQFTTDFGQ 549
PG GAIEM+W +++++L EP +T D A+ +PTV++ DL K QFT DFGQ
Sbjct: 378 PGDDGAIEMSWTDIEADELKEPDLTIKDFLKAIKSTRPTVNEDDLLKQEQFTRDFGQ 434
>sp|Q5XIK7|KATL1_RAT Katanin p60 ATPase-containing subunit A-like 1 (Katanin p60 subunit
A-like 1) (p60 katanin-like 1)
Length = 488
Score = 91.7 bits (226), Expect = 1e-18
Identities = 60/172 (34%), Positives = 89/172 (51%)
Frame = +1
Query: 31 VLILAATNIPWALDSAIRRRFEKRIYIALPEAGARAEMFKLSMGNKPGQHDLTPQNFSEL 210
V++LAATN PW +D A+RRR EKRIYI LP A RAE+ K+S+ D+ + ++
Sbjct: 350 VMVLAATNFPWDIDEALRRRLEKRIYIPLPTAKGRAELLKISLREVELDPDI---HLEDI 406
Query: 211 GHLSEGYSGADISIAVRDALMVPVRKVQTATHFRRVRGPSPTDPSVIVSDLLTPCSPGSP 390
+EGYSGADI+ RDA ++ +R RR+ G SP + +
Sbjct: 407 AEKTEGYSGADITNICRDASLMAMR--------RRINGLSPEEIRAL------------- 445
Query: 391 GAIEMNWQTVDSEKLLEPCVTFADMKLALSRNKPTVHQKDLEKLIQFTTDFG 546
S++ L+ VT D++LAL + +V DLEK ++ +FG
Sbjct: 446 -----------SKEELQMPVTRGDLELALKKIAKSVSAADLEKYEKWMVEFG 486
>sp|Q8K0T4|KATL1_MOUSE Katanin p60 ATPase-containing subunit A-like 1 (Katanin p60 subunit
A-like 1) (p60 katanin-like 1)
Length = 488
Score = 90.9 bits (224), Expect = 2e-18
Identities = 60/172 (34%), Positives = 89/172 (51%)
Frame = +1
Query: 31 VLILAATNIPWALDSAIRRRFEKRIYIALPEAGARAEMFKLSMGNKPGQHDLTPQNFSEL 210
V++LAATN PW +D A+RRR EKRIYI LP A RAE+ K+S+ D+ + ++
Sbjct: 350 VMVLAATNFPWDIDEALRRRLEKRIYIPLPTAKGRAELLKISLREVELDPDV---HLEDI 406
Query: 211 GHLSEGYSGADISIAVRDALMVPVRKVQTATHFRRVRGPSPTDPSVIVSDLLTPCSPGSP 390
+EGYSGADI+ RDA ++ +R RR+ G SP + +
Sbjct: 407 ADKTEGYSGADITNICRDASLMAMR--------RRINGLSPEEIRAL------------- 445
Query: 391 GAIEMNWQTVDSEKLLEPCVTFADMKLALSRNKPTVHQKDLEKLIQFTTDFG 546
S++ L+ VT D++LAL + +V DLEK ++ +FG
Sbjct: 446 -----------SKEELQMPVTRGDLELALKKIAKSVSAADLEKYEKWMVEFG 486
>sp|Q9BW62|KATL1_HUMAN Katanin p60 ATPase-containing subunit A-like 1 (Katanin p60 subunit
A-like 1) (p60 katanin-like 1)
Length = 490
Score = 89.0 bits (219), Expect = 9e-18
Identities = 60/172 (34%), Positives = 88/172 (51%)
Frame = +1
Query: 31 VLILAATNIPWALDSAIRRRFEKRIYIALPEAGARAEMFKLSMGNKPGQHDLTPQNFSEL 210
V++LAATN PW +D A+RRR EKRIYI LP A RAE+ K+++ D+ ++ +E
Sbjct: 352 VMVLAATNFPWDIDEALRRRLEKRIYIPLPTAKGRAELLKINLREVELDPDIQLEDIAE- 410
Query: 211 GHLSEGYSGADISIAVRDALMVPVRKVQTATHFRRVRGPSPTDPSVIVSDLLTPCSPGSP 390
EGYSGADI+ RDA ++ +R RR+ G SP + +
Sbjct: 411 --KIEGYSGADITNVCRDASLMAMR--------RRINGLSPEEIRAL------------- 447
Query: 391 GAIEMNWQTVDSEKLLEPCVTFADMKLALSRNKPTVHQKDLEKLIQFTTDFG 546
S++ L+ VT D +LAL + +V DLEK ++ +FG
Sbjct: 448 -----------SKEELQMPVTKGDFELALKKIAKSVSAADLEKYEKWMVEFG 488
>sp|P39955|SAP1_YEAST SAP1 protein
Length = 897
Score = 85.9 bits (211), Expect = 8e-17
Identities = 43/91 (47%), Positives = 61/91 (67%)
Frame = +1
Query: 13 EADNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAGARAEMFKLSMGNKPGQHDLTP 192
+ D+ VL+LAATN+PW++D A RRRF +R YI LPE R FK + ++ +H LT
Sbjct: 756 DEDDTRVLVLAATNLPWSIDEAARRRFVRRQYIPLPEDQTRHVQFKKLLSHQ--KHTLTE 813
Query: 193 QNFSELGHLSEGYSGADISIAVRDALMVPVR 285
+F EL ++EGYSG+DI+ +DA M P+R
Sbjct: 814 SDFDELVKITEGYSGSDITSLAKDAAMGPLR 844
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 76,713,402
Number of Sequences: 369166
Number of extensions: 1669432
Number of successful extensions: 6169
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5665
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6054
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 4877307000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)