Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_022_L02
(839 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q26499|IM23_SCHHA 23 kDa integral membrane protein (Sh23) 64 7e-10
sp|P27591|IM23_SCHJA 23 kDa integral membrane protein (SJ23) 63 9e-10
sp|Q28709|CD63_RABIT CD63 antigen 61 4e-09
sp|P08962|CD63_HUMAN CD63 antigen (Melanoma-associated anti... 60 7e-09
sp|O70401|TSN6_MOUSE Tetraspanin-6 (Tspan-6) (Transmembrane... 60 7e-09
sp|P61171|CD151_MACMU Platelet-endothelial tetraspan antige... 60 1e-08
sp|P48509|CD151_HUMAN Platelet-endothelial tetraspan antige... 59 2e-08
sp|O43657|TSN6_HUMAN Tetraspanin-6 (Tspan-6) (Transmembrane... 58 3e-08
sp|O35566|C151_MOUSE Platelet-endothelial tetraspan antigen... 58 3e-08
sp|P41731|CD63_MOUSE CD63 antigen 58 4e-08
>sp|Q26499|IM23_SCHHA 23 kDa integral membrane protein (Sh23)
Length = 218
Score = 63.5 bits (153), Expect = 7e-10
Identities = 49/213 (23%), Positives = 83/213 (38%), Gaps = 4/213 (1%)
Frame = +1
Query: 22 MRCLRVITAVFTGMFMIFSVALITIGILCKSLWSDYLLILQSTMGSVPXXXXXXXXXXXX 201
MRCL+ V + ++ S+ LI G + +S Y L + P
Sbjct: 8 MRCLKSCVFVLNIICLLCSLVLIGAGAYVEVKFSQYGDNLHKVWQAAPIAIIVVGVIILI 67
Query: 202 XTIVGLFGTIMKNWILLVVYSGLLAVIIFVEVGAAITCGVFQTQLNFTVKLGMKNVMSNN 381
+ +G G I +N +L +Y+ L +++ E+ AAI V++ +++ + M +
Sbjct: 68 VSFLGCCGAIKENVCMLYMYAFFLIILLIAELAAAIVAVVYKDRIDSEIDALMTGALDKP 127
Query: 382 TM----VMDAVQNKFSCCGINGYQDYXXXXXXXXXXXXXXXXXXXXXXXGCLPVFVSVSS 549
T MD +Q+ F CCG G QDY GC+PVF +
Sbjct: 128 TPEITEFMDLIQSSFHCCGAKGPQDY------GPNIPASCRGETTVYHEGCVPVFGAFLK 181
Query: 550 XXXXXXXXXXXXXXXFETLSIIVIARLYNLLKQ 648
F+ LSI++ L +K+
Sbjct: 182 RNLVIVACVAFGVCFFQLLSIVIACCLGRQIKE 214
>sp|P27591|IM23_SCHJA 23 kDa integral membrane protein (SJ23)
Length = 218
Score = 63.2 bits (152), Expect = 9e-10
Identities = 38/146 (26%), Positives = 65/146 (44%), Gaps = 4/146 (2%)
Frame = +1
Query: 22 MRCLRVITAVFTGMFMIFSVALITIGILCKSLWSDYLLILQSTMGSVPXXXXXXXXXXXX 201
MRCL+ + + ++ S+ LI G + +S Y L + P
Sbjct: 8 MRCLKSCVFILNIICLLCSLVLIGAGAYVEVKFSQYEANLHKVWQAAPIAIIVVGVVILI 67
Query: 202 XTIVGLFGTIMKNWILLVVYSGLLAVIIFVEVGAAITCGVFQTQLNFTVKLGMKNVMSNN 381
+ +G G I +N +L +Y+ L V++ E+ AAI V++ +++ + M + N
Sbjct: 68 VSFLGCCGAIKENVCMLYMYAFFLIVLLIAELVAAIVAVVYKDKIDDEINTLMTGALENP 127
Query: 382 ----TMVMDAVQNKFSCCGINGYQDY 447
T MD +Q F CCG+ G DY
Sbjct: 128 NEEITATMDKIQTSFHCCGVKGPDDY 153
>sp|Q28709|CD63_RABIT CD63 antigen
Length = 238
Score = 60.8 bits (146), Expect = 4e-09
Identities = 38/148 (25%), Positives = 72/148 (48%), Gaps = 6/148 (4%)
Frame = +1
Query: 22 MRCLRVITAVFTGMFMIFSVALITIGILCKSLWSDYLLILQSTMGSVPXXXXXXXXXXXX 201
M+C++ + V F +V LI +G+ + + S + + +P
Sbjct: 7 MKCVKFLLYVLLLAFCACAVGLIAVGVGAQLVLSQTITHGATPGSLLPVVIIAVGAFLFL 66
Query: 202 XTIVGLFGTIMKNWILLVVYSGLLAVIIFVEVGAAITCGVFQ----TQLNFTVKLGMKNV 369
VG GT +N+ L++ ++ L++I+ VEV AAI VF+ ++ N + M+N
Sbjct: 67 VAFVGCCGTCKENYCLMITFAIFLSLIMLVEVAAAIAGYVFRDKVMSEFNKDFRQQMQNY 126
Query: 370 MSNN--TMVMDAVQNKFSCCGINGYQDY 447
++N +++D +Q F+CCG Y D+
Sbjct: 127 STDNQTALILDRMQKDFTCCGAANYTDW 154
>sp|P08962|CD63_HUMAN CD63 antigen (Melanoma-associated antigen ME491)
(Lysosome-associated membrane glycoprotein 3) (LAMP-3)
(Ocular melanoma-associated antigen) (OMA81H)
(Granulophysin) (Tetraspanin-30) (Tspan-30)
Length = 238
Score = 60.1 bits (144), Expect = 7e-09
Identities = 42/149 (28%), Positives = 72/149 (48%), Gaps = 7/149 (4%)
Frame = +1
Query: 22 MRCLRVITAVFTGMFMIFSVALITIGILCKSLWSDYLLILQSTMGSV-PXXXXXXXXXXX 198
M+C++ + V F +V LI +G+ + + S +I +T GS+ P
Sbjct: 7 MKCVKFLLYVLLLAFCACAVGLIAVGVGAQLVLSQ-TIIQGATPGSLLPVVIIAVGVFLF 65
Query: 199 XXTIVGLFGTIMKNWILLVVYSGLLAVIIFVEVGAAITCGVFQ----TQLNFTVKLGMKN 366
VG G +N+ L++ ++ L++I+ VEV AAI VF+ ++ N + M+N
Sbjct: 66 LVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAAAIAGYVFRDKVMSEFNNNFRQQMEN 125
Query: 367 VMSNN--TMVMDAVQNKFSCCGINGYQDY 447
NN ++D +Q F CCG Y D+
Sbjct: 126 YPKNNHTASILDRMQADFKCCGAANYTDW 154
>sp|O70401|TSN6_MOUSE Tetraspanin-6 (Tspan-6) (Transmembrane 4 superfamily member 6)
Length = 245
Score = 60.1 bits (144), Expect = 7e-09
Identities = 37/147 (25%), Positives = 65/147 (44%), Gaps = 7/147 (4%)
Frame = +1
Query: 28 CLRVITAVFTGMFMIFSVALITIGILCKSLWSDYLLILQSTMGSVPXXXXXXXXXXXXXT 207
CL+ + ++T +F I V L+ +GI K +Y +L +VP
Sbjct: 16 CLKSVLLIYTFIFWITGVILLAVGIWGKVSLENYFSLLNEKATNVPFVLIGTGTVIILLG 75
Query: 208 IVGLFGTIMKNWILLVVYSGLLAVIIFVEVGAAITCGVFQTQLNFTVKLGMKNVMSN--- 378
G F T + +L +Y+ L +I VE+ AAI VF+ ++ + K +N +
Sbjct: 76 TFGCFATCRTSAWMLKLYAMFLTLIFLVELVAAIVGFVFRHEIKNSFKSNYENALKEYNS 135
Query: 379 ----NTMVMDAVQNKFSCCGINGYQDY 447
+ +D +Q+ CCG+ Y D+
Sbjct: 136 TGDYRSEAVDKIQSTLHCCGVTNYGDW 162
>sp|P61171|CD151_MACMU Platelet-endothelial tetraspan antigen 3 (PETA-3) (CD151 antigen)
sp|P61170|CD151_CERAE Platelet-endothelial tetraspan antigen 3 (PETA-3) (CD151 antigen)
Length = 253
Score = 59.7 bits (143), Expect = 1e-08
Identities = 40/150 (26%), Positives = 69/150 (46%), Gaps = 10/150 (6%)
Frame = +1
Query: 28 CLRVITAVFTGMFMIFSVALITIGILCKSLWSDYLLILQS-TMGSVPXXXXXXXXXXXXX 204
CL+ + + F + +A++ +GI +L SDY+ +L S T +
Sbjct: 15 CLKYLLFTYNCCFWLAGLAVMAVGIWTLALKSDYISLLASGTYLATAYILVVAGAVVMVT 74
Query: 205 TIVGLFGTIMKNWILLVVYSGLLAVIIFVEVGAAITCGVFQTQLNFTVKLGMKNVMSNN- 381
++G T + LL +Y LL +I +E+ A + V+ QLN +K +K+ M+
Sbjct: 75 GVLGCCATFKERRNLLRLYFILLLIIFLLEIIAGVLAYVYYQQLNTELKENLKDTMAKRY 134
Query: 382 --------TMVMDAVQNKFSCCGINGYQDY 447
T +D +Q +F CCG N QD+
Sbjct: 135 HQPGHEAVTSAVDQLQQEFHCCGSNNSQDW 164
>sp|P48509|CD151_HUMAN Platelet-endothelial tetraspan antigen 3 (PETA-3) (GP27) (Membrane
glycoprotein SFA-1) (CD151 antigen) (Tetraspanin-24)
(Tspan-24)
Length = 253
Score = 58.5 bits (140), Expect = 2e-08
Identities = 40/150 (26%), Positives = 68/150 (45%), Gaps = 10/150 (6%)
Frame = +1
Query: 28 CLRVITAVFTGMFMIFSVALITIGILCKSLWSDYLLILQS-TMGSVPXXXXXXXXXXXXX 204
CL+ + + F + +A++ +GI +L SDY+ +L S T +
Sbjct: 15 CLKYLLFTYNCCFWLAGLAVMAVGIWTLALKSDYISLLASGTYLATAYILVVAGTVVMVT 74
Query: 205 TIVGLFGTIMKNWILLVVYSGLLAVIIFVEVGAAITCGVFQTQLNFTVKLGMKNVMSNN- 381
++G T + LL +Y LL +I +E+ A I + QLN +K +K+ M+
Sbjct: 75 GVLGCCATFKERRNLLRLYFILLLIIFLLEIIAGILAYAYYQQLNTELKENLKDTMTRRY 134
Query: 382 --------TMVMDAVQNKFSCCGINGYQDY 447
T +D +Q +F CCG N QD+
Sbjct: 135 HQSGHEAVTSAVDQLQQEFHCCGSNNSQDW 164
>sp|O43657|TSN6_HUMAN Tetraspanin-6 (Tspan-6) (Transmembrane 4 superfamily member 6)
(T245 protein) (Tetraspanin TM4-D) (A15 homolog)
Length = 245
Score = 58.2 bits (139), Expect = 3e-08
Identities = 35/147 (23%), Positives = 64/147 (43%), Gaps = 7/147 (4%)
Frame = +1
Query: 28 CLRVITAVFTGMFMIFSVALITIGILCKSLWSDYLLILQSTMGSVPXXXXXXXXXXXXXT 207
C + + ++T +F I V L+ +GI K +Y +L +VP
Sbjct: 16 CFKSVLLIYTFIFWITGVILLAVGIWGKVSLENYFSLLNEKATNVPFVLIATGTVIILLG 75
Query: 208 IVGLFGTIMKNWILLVVYSGLLAVIIFVEVGAAITCGVFQTQLNFTVKLGMKNVMSN--- 378
G F T + +L +Y+ L ++ VE+ AAI VF+ ++ + K + +
Sbjct: 76 TFGCFATCRASAWMLKLYAMFLTLVFLVELVAAIVGFVFRHEIKNSFKNNYEKALKQYNS 135
Query: 379 ----NTMVMDAVQNKFSCCGINGYQDY 447
+ +D +QN CCG+ Y+D+
Sbjct: 136 TGDYRSHAVDKIQNTLHCCGVTDYRDW 162
>sp|O35566|C151_MOUSE Platelet-endothelial tetraspan antigen 3 (PETA-3) (GP27) (Membrane
glycoprotein SFA-1) (CD151 antigen)
Length = 253
Score = 58.2 bits (139), Expect = 3e-08
Identities = 40/150 (26%), Positives = 68/150 (45%), Gaps = 10/150 (6%)
Frame = +1
Query: 28 CLRVITAVFTGMFMIFSVALITIGILCKSLWSDYL-LILQSTMGSVPXXXXXXXXXXXXX 204
CL+ + + F + +A++ +GI +L SDY+ L+ ST +
Sbjct: 15 CLKYLLFTYNCCFWLAGLAVMAVGIWTLALKSDYISLLASSTYLATAYILVVAGVVVMVT 74
Query: 205 TIVGLFGTIMKNWILLVVYSGLLAVIIFVEVGAAITCGVFQTQLNFTVKLGMKNVMSNN- 381
++G T + LL +Y LL +I +E+ A I V+ QLN +K +K+ M
Sbjct: 75 GVLGCCATFKERRNLLRLYFILLLIIFLLEIIAGILAYVYYQQLNTELKENLKDTMVKRY 134
Query: 382 --------TMVMDAVQNKFSCCGINGYQDY 447
+ +D +Q +F CCG N QD+
Sbjct: 135 HQSGHEGVSSAVDKLQQEFHCCGSNNSQDW 164
>sp|P41731|CD63_MOUSE CD63 antigen
Length = 238
Score = 57.8 bits (138), Expect = 4e-08
Identities = 39/149 (26%), Positives = 74/149 (49%), Gaps = 7/149 (4%)
Frame = +1
Query: 22 MRCLRVITAVFTGMFMIFSVALITIGILCKSLWSDYLLILQSTMGSV-PXXXXXXXXXXX 198
M+C++ + V F +V LI IG+ + + + ++T GS+ P
Sbjct: 7 MKCVKFLLYVLLLAFCACAVGLIAIGVAVQVVLKQ-AITHETTAGSLLPVVIIAVGAFLF 65
Query: 199 XXTIVGLFGTIMKNWILLVVYSGLLAVIIFVEVGAAITCGVFQTQL----NFTVKLGMKN 366
VG G +N+ L++ ++ L++I+ VEV AI VF+ Q+ N + + M+N
Sbjct: 66 LVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAVAIAGYVFRDQVKSEFNKSFQQQMQN 125
Query: 367 VMSNN--TMVMDAVQNKFSCCGINGYQDY 447
+ +N ++D +Q + +CCG + Y D+
Sbjct: 126 YLKDNKTATILDKLQKENNCCGASNYTDW 154
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 98,881,263
Number of Sequences: 369166
Number of extensions: 2044402
Number of successful extensions: 5499
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5246
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5479
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8197207050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)