Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_022_K23
(312 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O08679|MARK2_RAT Serine/threonine-protein kinase MARK2 (... 62 4e-10
sp|Q05512|MARK2_MOUSE Serine/threonine-protein kinase MARK2... 62 4e-10
sp|Q7KZI7|MARK2_HUMAN Serine/threonine-protein kinase MARK2... 62 4e-10
sp|Q8CIP4|MARK4_MOUSE MAP/microtubule affinity-regulating k... 59 3e-09
sp|Q96L34|MARK4_HUMAN MAP/microtubule affinity-regulating k... 59 3e-09
sp|Q8VHF0|MARK3_RAT MAP/microtubule affinity-regulating kin... 58 6e-09
sp|Q03141|MARK3_MOUSE MAP/microtubule affinity-regulating k... 58 6e-09
sp|P27448|MARK3_HUMAN MAP/microtubule affinity-regulating k... 58 6e-09
sp|O94547|SRK1_SCHPO Serine/threonine-protein kinase srk1 (... 57 1e-08
sp|Q8ICR0|CDPK2_PLAF7 Calcium-dependent protein kinase 2 (P... 57 1e-08
>sp|O08679|MARK2_RAT Serine/threonine-protein kinase MARK2 (MAP/microtubule
affinity-regulating kinase 2) (ELKL Motif Kinase) (EMK1)
Length = 722
Score = 62.0 bits (149), Expect = 4e-10
Identities = 35/106 (33%), Positives = 58/106 (54%), Gaps = 4/106 (3%)
Frame = +3
Query: 6 GNKSSESLIEQ----KYKILNNVGEGGFCVVMRGLNHITNEAVAIKKIDKSKPVYKKFKG 173
G S+ S EQ Y++L +G+G F V + +T + VA+K IDK
Sbjct: 37 GRNSATSADEQPHIGNYRLLKTIGKGNFAKVKLARHILTGKEVAVKIIDK---------- 86
Query: 174 TEAKTKMNNIMIREAAVMQALDHPNLMKLIELIDDGKHIYLVLQFA 311
T+ + + RE +M+ L+HPN++KL E+I+ K +YLV+++A
Sbjct: 87 TQLNSSSLQKLFREVRIMKVLNHPNIVKLFEVIETEKTLYLVMEYA 132
>sp|Q05512|MARK2_MOUSE Serine/threonine-protein kinase MARK2 (MAP/microtubule
affinity-regulating kinase 2) (ELKL Motif Kinase) (EMK1)
Length = 776
Score = 62.0 bits (149), Expect = 4e-10
Identities = 35/106 (33%), Positives = 58/106 (54%), Gaps = 4/106 (3%)
Frame = +3
Query: 6 GNKSSESLIEQ----KYKILNNVGEGGFCVVMRGLNHITNEAVAIKKIDKSKPVYKKFKG 173
G S+ S EQ Y++L +G+G F V + +T + VA+K IDK
Sbjct: 37 GRNSATSADEQPHIGNYRLLKTIGKGNFAKVKLARHILTGKEVAVKIIDK---------- 86
Query: 174 TEAKTKMNNIMIREAAVMQALDHPNLMKLIELIDDGKHIYLVLQFA 311
T+ + + RE +M+ L+HPN++KL E+I+ K +YLV+++A
Sbjct: 87 TQLNSSSLQKLFREVRIMKVLNHPNIVKLFEVIETEKTLYLVMEYA 132
>sp|Q7KZI7|MARK2_HUMAN Serine/threonine-protein kinase MARK2 (MAP/microtubule
affinity-regulating kinase 2) (ELKL motif kinase) (EMK1)
(PAR1 homolog)
Length = 788
Score = 62.0 bits (149), Expect = 4e-10
Identities = 35/106 (33%), Positives = 58/106 (54%), Gaps = 4/106 (3%)
Frame = +3
Query: 6 GNKSSESLIEQ----KYKILNNVGEGGFCVVMRGLNHITNEAVAIKKIDKSKPVYKKFKG 173
G S+ S EQ Y++L +G+G F V + +T + VA+K IDK
Sbjct: 37 GRNSATSADEQPHIGNYRLLKTIGKGNFAKVKLARHILTGKEVAVKIIDK---------- 86
Query: 174 TEAKTKMNNIMIREAAVMQALDHPNLMKLIELIDDGKHIYLVLQFA 311
T+ + + RE +M+ L+HPN++KL E+I+ K +YLV+++A
Sbjct: 87 TQLNSSSLQKLFREVRIMKVLNHPNIVKLFEVIETEKTLYLVMEYA 132
>sp|Q8CIP4|MARK4_MOUSE MAP/microtubule affinity-regulating kinase 4
Length = 752
Score = 59.3 bits (142), Expect = 3e-09
Identities = 31/90 (34%), Positives = 50/90 (55%)
Frame = +3
Query: 42 YKILNNVGEGGFCVVMRGLNHITNEAVAIKKIDKSKPVYKKFKGTEAKTKMNNIMIREAA 221
Y++L +G+G F V + +T VAIK IDK T+ + RE
Sbjct: 59 YRLLRTIGKGNFAKVKLARHILTGREVAIKIIDK----------TQLNPSSLQKLFREVR 108
Query: 222 VMQALDHPNLMKLIELIDDGKHIYLVLQFA 311
+M+ L+HPN++KL E+I+ K +YLV+++A
Sbjct: 109 IMKGLNHPNIVKLFEVIETEKTLYLVMEYA 138
>sp|Q96L34|MARK4_HUMAN MAP/microtubule affinity-regulating kinase 4 (MAP/microtubule
affinity-regulating kinase-like 1)
Length = 752
Score = 59.3 bits (142), Expect = 3e-09
Identities = 31/90 (34%), Positives = 50/90 (55%)
Frame = +3
Query: 42 YKILNNVGEGGFCVVMRGLNHITNEAVAIKKIDKSKPVYKKFKGTEAKTKMNNIMIREAA 221
Y++L +G+G F V + +T VAIK IDK T+ + RE
Sbjct: 59 YRLLRTIGKGNFAKVKLARHILTGREVAIKIIDK----------TQLNPSSLQKLFREVR 108
Query: 222 VMQALDHPNLMKLIELIDDGKHIYLVLQFA 311
+M+ L+HPN++KL E+I+ K +YLV+++A
Sbjct: 109 IMKGLNHPNIVKLFEVIETEKTLYLVMEYA 138
>sp|Q8VHF0|MARK3_RAT MAP/microtubule affinity-regulating kinase 3
Length = 797
Score = 58.2 bits (139), Expect = 6e-09
Identities = 30/90 (33%), Positives = 50/90 (55%)
Frame = +3
Query: 42 YKILNNVGEGGFCVVMRGLNHITNEAVAIKKIDKSKPVYKKFKGTEAKTKMNNIMIREAA 221
Y++L +G+G F V + +T VAIK IDK T+ + RE
Sbjct: 56 YRLLKTIGKGNFAKVKLARHILTGREVAIKIIDK----------TQLNPTSLQKLFREVR 105
Query: 222 VMQALDHPNLMKLIELIDDGKHIYLVLQFA 311
+M+ L+HPN++KL E+I+ K +YL++++A
Sbjct: 106 IMKILNHPNIVKLFEVIETEKTLYLIMEYA 135
>sp|Q03141|MARK3_MOUSE MAP/microtubule affinity-regulating kinase 3 (MPK-10) (ELKL motif
kinase 2)
Length = 753
Score = 58.2 bits (139), Expect = 6e-09
Identities = 30/90 (33%), Positives = 50/90 (55%)
Frame = +3
Query: 42 YKILNNVGEGGFCVVMRGLNHITNEAVAIKKIDKSKPVYKKFKGTEAKTKMNNIMIREAA 221
Y++L +G+G F V + +T VAIK IDK T+ + RE
Sbjct: 56 YRLLKTIGKGNFAKVKLARHILTGREVAIKIIDK----------TQLNPTSLQKLFREVR 105
Query: 222 VMQALDHPNLMKLIELIDDGKHIYLVLQFA 311
+M+ L+HPN++KL E+I+ K +YL++++A
Sbjct: 106 IMKILNHPNIVKLFEVIETEKTLYLIMEYA 135
>sp|P27448|MARK3_HUMAN MAP/microtubule affinity-regulating kinase 3 (Cdc25C-associated
protein kinase 1) (cTAK1) (C-TAK1) (Serine/threonine
protein kinase p78) (Ser/Thr protein kinase PAR-1)
(Protein kinase STK10)
Length = 776
Score = 58.2 bits (139), Expect = 6e-09
Identities = 30/90 (33%), Positives = 50/90 (55%)
Frame = +3
Query: 42 YKILNNVGEGGFCVVMRGLNHITNEAVAIKKIDKSKPVYKKFKGTEAKTKMNNIMIREAA 221
Y++L +G+G F V + +T VAIK IDK T+ + RE
Sbjct: 56 YRLLKTIGKGNFAKVKLARHILTGREVAIKIIDK----------TQLNPTSLQKLFREVR 105
Query: 222 VMQALDHPNLMKLIELIDDGKHIYLVLQFA 311
+M+ L+HPN++KL E+I+ K +YL++++A
Sbjct: 106 IMKILNHPNIVKLFEVIETEKTLYLIMEYA 135
>sp|O94547|SRK1_SCHPO Serine/threonine-protein kinase srk1 (Sty1-regulated kinase 1)
Length = 580
Score = 57.4 bits (137), Expect = 1e-08
Identities = 26/92 (28%), Positives = 56/92 (60%)
Frame = +3
Query: 36 QKYKILNNVGEGGFCVVMRGLNHITNEAVAIKKIDKSKPVYKKFKGTEAKTKMNNIMIRE 215
++Y +L +G+G F V + +++ T E VAIK + +++P + + ++ +++E
Sbjct: 122 EQYTLLQKMGDGAFSNVYKAIHNRTGEKVAIKVVQRAQPNTDPRDPRKRQGVESHNILKE 181
Query: 216 AAVMQALDHPNLMKLIELIDDGKHIYLVLQFA 311
+M+ + HPN+++L+E I ++ YLVL+ A
Sbjct: 182 VQIMRRVKHPNIIQLLEFIQTPEYYYLVLELA 213
>sp|Q8ICR0|CDPK2_PLAF7 Calcium-dependent protein kinase 2 (PfCDPK2)
Length = 509
Score = 57.0 bits (136), Expect = 1e-08
Identities = 30/94 (31%), Positives = 53/94 (56%), Gaps = 2/94 (2%)
Frame = +3
Query: 30 IEQKYKILNNVGEGGFCVVMRGLNHITNEAVAIKKIDKSKPVYKKFKGTEAKTKMNNI-- 203
+E KY I +G+G + V +G++ +TN+ AIK+ E K ++ NI
Sbjct: 68 LEDKYIIDEKLGQGTYGCVYKGIDKVTNQLYAIKE--------------EKKDRLKNINR 113
Query: 204 MIREAAVMQALDHPNLMKLIELIDDGKHIYLVLQ 305
+E +M+ LDHPN++KL E ++ +IYL+++
Sbjct: 114 FFQEIEIMKKLDHPNIVKLYETYENDNYIYLIME 147
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,667,478
Number of Sequences: 369166
Number of extensions: 485148
Number of successful extensions: 2914
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2614
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2650
length of database: 68,354,980
effective HSP length: 72
effective length of database: 55,054,060
effective search space used: 1706675860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)