Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_022_K22
(742 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P34732|SEC18_CANAL Vesicular-fusion protein SEC18 34 0.47
sp|P58132|RPOC2_ASTLO DNA-directed RNA polymerase beta'' ch... 34 0.47
sp|Q8K9K0|FLGK_BUCAP Flagellar hook-associated protein 1 (H... 33 1.0
sp|P03456|HEMA_IATKP Hemagglutinin precursor [Contains: Hem... 32 1.4
sp|Q8K9E8|Y389_BUCAP Hypothetical UPF0169 protein BUsg389 32 1.4
sp|P34771|RK23_ASTLO Plastid 50S ribosomal protein L23 31 3.0
sp|P40233|CKI1_SCHPO Casein kinase I homolog cki1 31 3.0
sp|P23643|VPS3_YEAST Vacuolar protein sorting-associated pr... 31 4.0
sp|P34050|VP43_NPVAC 43 KD PROTEIN 31 4.0
sp|P36083|YKH5_YEAST Hypothetical 52.1 kDa protein in SMY1-... 30 5.2
>sp|P34732|SEC18_CANAL Vesicular-fusion protein SEC18
Length = 794
Score = 33.9 bits (76), Expect = 0.47
Identities = 24/78 (30%), Positives = 38/78 (48%), Gaps = 7/78 (8%)
Frame = +1
Query: 265 LIDKVIDKLNNMKDPSFIFGMKLLSK-------NWNMIFNNKFCEWFSKLISDKSDVRKC 423
LI + + K+ N K+P + G ++LSK N +F + E+ KL + SD+
Sbjct: 325 LIARKLSKMLNGKEPKIVNGPEMLSKYVGASEENIRNLFKDAEAEY--KLKGEDSDLHVI 382
Query: 424 LFTIKDSIHALNRSGKQD 477
+F DS+ SGK D
Sbjct: 383 IFDELDSVFKQRGSGKSD 400
>sp|P58132|RPOC2_ASTLO DNA-directed RNA polymerase beta'' chain (PEP) (Plastid-encoded RNA
polymerase beta'' subunit) (RNA polymerase beta''
subunit)
Length = 817
Score = 33.9 bits (76), Expect = 0.47
Identities = 44/158 (27%), Positives = 69/158 (43%), Gaps = 8/158 (5%)
Frame = +1
Query: 16 ISKIPNYVIPETYFNNVMTVFNKLDGQQASINNDFLT--NFVAYINRAYPSKKSEYIIEI 189
+ KI + + + Y N FN L +N +F+T F + KK Y I
Sbjct: 440 VKKIKSNISGKKYINKKNKKFNNL---YWILNANFITFNKFYHKLTDKLNFKKKSYTISK 496
Query: 190 WFYCVCHDINNEDLNSIMDSEVSQQLIDKVIDKLNNMK------DPSFIFGMKLLSKNWN 351
N +D N S+ + QL + + LNNM+ + FIF ++L+KN
Sbjct: 497 ---------NMQDKNK--KSKKNLQLKISIKNILNNMEKKKSKTNKKFIFINEILNKNKT 545
Query: 352 MIFNNKFCEWFSKLISDKSDVRKCLFTIKDSIHALNRS 465
+I N E K+I++K + K F +KD I NR+
Sbjct: 546 IILNKPKKE---KIINNKWKIGK--FILKDEITNKNRN 578
>sp|Q8K9K0|FLGK_BUCAP Flagellar hook-associated protein 1 (HAP1)
Length = 544
Score = 32.7 bits (73), Expect = 1.0
Identities = 43/208 (20%), Positives = 86/208 (41%), Gaps = 6/208 (2%)
Frame = +1
Query: 19 SKIPNYVIPETYFNNVMTVFNKLDGQ-QASINNDFLTNFVAYINRAYPSKKSEYIIEIWF 195
+KI Y+ E F +F L Q +SI ND + N N IE
Sbjct: 83 TKIEEYLKLEDLFGEKSNIFTLLINQLYSSIENDIVNNHGNVFNEN---------IENNL 133
Query: 196 YCVCHDINNEDLN-SIMDSEVSQQLIDKVIDK---LNNMKDPSFIFGMKLLSKNWNMIFN 363
+ H++ N D S ++ +V + + +K+ +N + D + ++ N I
Sbjct: 134 NTIIHELKNFDEKLSFLERDVKESIKEKIKQANILINKIYDTNIDIRFFPTAQLPNRI-- 191
Query: 364 NKFCEWFSKLISDKSDVRKCLFTIKDSIHALNRSGKQDIFDSFYLLAICVMSYLNKKSLF 543
+ F + KLI + +D+ ++S + + +C++ NKK+L
Sbjct: 192 DSFIDKRDKLIDELNDIIGVKVIKENSTFKVCLNN-----------GMCIIDDYNKKNLM 240
Query: 544 TCSKNVYEHFLK-NYFPDIKRGLLKVDN 624
T + + + ++ YF D ++ L K+++
Sbjct: 241 TLTSDTDDKYISVGYFDDNEQRLKKIEH 268
>sp|P03456|HEMA_IATKP Hemagglutinin precursor [Contains: Hemagglutinin HA1 chain;
Hemagglutinin HA2 chain]
Length = 565
Score = 32.3 bits (72), Expect = 1.4
Identities = 34/134 (25%), Positives = 59/134 (44%)
Frame = +1
Query: 208 HDINNEDLNSIMDSEVSQQLIDKVIDKLNNMKDPSFIFGMKLLSKNWNMIFNNKFCEWFS 387
H N+E D + +Q+ IDK+ +K+NN+ D +++ + ++ N++F
Sbjct: 368 HHSNSEGTGMAADQKSTQEAIDKITNKVNNIVDK--------MNREFEVV-NHEF----- 413
Query: 388 KLISDKSDVRKCLFTIKDSIHALNRSGKQDIFDSFYLLAICVMSYLNKKSLFTCSKNVYE 567
S+V K + I D I I D + A ++ N+K+L NV
Sbjct: 414 ------SEVEKRINMINDKI-------DDQIEDLWAYNAELLVLLENQKTLDEHDSNV-- 458
Query: 568 HFLKNYFPDIKRGL 609
KN F ++KR L
Sbjct: 459 ---KNLFDEVKRRL 469
>sp|Q8K9E8|Y389_BUCAP Hypothetical UPF0169 protein BUsg389
Length = 243
Score = 32.3 bits (72), Expect = 1.4
Identities = 29/105 (27%), Positives = 49/105 (46%), Gaps = 10/105 (9%)
Frame = +1
Query: 301 KDPSFIFGMKLLS--------KNWNMIFNNKFCEWFSKLISDKSDVRKCLFTIK--DSIH 450
K+ FIF + LS KN+N+I NN + K + +K + K +F +K ++ H
Sbjct: 4 KNTVFIFMILFLSFAIYSKNLKNYNLILNNYLYQKCRKELKEK-NFYKAIFDLKKIENNH 62
Query: 451 ALNRSGKQDIFDSFYLLAICVMSYLNKKSLFTCSKNVYEHFLKNY 585
A+N F++ + + +Y T KN+ E F+K Y
Sbjct: 63 AIN-------FNNDKIKMNLIYAYYKVSDFNTAEKNI-EEFIKKY 99
>sp|P34771|RK23_ASTLO Plastid 50S ribosomal protein L23
Length = 98
Score = 31.2 bits (69), Expect = 3.0
Identities = 20/86 (23%), Positives = 43/86 (50%)
Frame = +1
Query: 181 IEIWFYCVCHDINNEDLNSIMDSEVSQQLIDKVIDKLNNMKDPSFIFGMKLLSKNWNMIF 360
+ I+ + + + + N ++ + +DK ++KL +IF +K+ S N I
Sbjct: 5 VRIFLPFLKYQVFTDKTNDLLKYNIYVFDVDKKLNKLQIKNIIEYIFNIKIYSIN-TYIK 63
Query: 361 NNKFCEWFSKLISDKSDVRKCLFTIK 438
NNK+C F+K+ K++ ++ +K
Sbjct: 64 NNKYC-CFNKIKGLKTNYKRAFIRLK 88
>sp|P40233|CKI1_SCHPO Casein kinase I homolog cki1
Length = 446
Score = 31.2 bits (69), Expect = 3.0
Identities = 17/59 (28%), Positives = 28/59 (47%)
Frame = +1
Query: 193 FYCVCHDINNEDLNSIMDSEVSQQLIDKVIDKLNNMKDPSFIFGMKLLSKNWNMIFNNK 369
FY H N ++ D + Q L KV+++LN +D +F +WN++ N K
Sbjct: 253 FYKYMHYARNLAFDATPDYDYLQGLFSKVLERLNTTEDENF---------DWNLLNNGK 302
>sp|P23643|VPS3_YEAST Vacuolar protein sorting-associated protein VPS3
Length = 1011
Score = 30.8 bits (68), Expect = 4.0
Identities = 33/140 (23%), Positives = 61/140 (43%), Gaps = 9/140 (6%)
Frame = +1
Query: 313 FIFGMKLLSKNW------NMIFNNKFCEWFSKLISDKSDVRKCLFTIKDSIHALNRSG-- 468
++FG K+ S+ W N++ K KL + ++ K L +K+ + N++G
Sbjct: 558 YMFGWKVYSEIWCFHGLINIVERLKSL----KLTNKCENILKMLLMMKNELKKKNKTGLL 613
Query: 469 KQDIFDSFYLLAICVMSY-LNKKSLFTCSKNVYEHFLKNYFPDIKRGLLKVDNKFKLKWT 645
D D + I + L KK T + F + + +I R + D+K
Sbjct: 614 TNDFDDIMKTIDITLFKLRLEKKETITV-----DMFERESYDEIIREINLHDDKLPRIEL 668
Query: 646 MVECLISSAEYEKALSVIKE 705
++E EY KAL++++E
Sbjct: 669 LIEIYKEKGEYLKALNLLRE 688
>sp|P34050|VP43_NPVAC 43 KD PROTEIN
Length = 363
Score = 30.8 bits (68), Expect = 4.0
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 2/49 (4%)
Frame = +1
Query: 43 PETYFNNVMTVFNKLDGQQASIN--NDFLTNFVAYINRAYPSKKSEYII 183
P+ YF+ +F KLD +IN + LT+F Y Y +K+++ +
Sbjct: 23 PKRYFHTNKVIFEKLDSYATNINRCRELLTDFFDYCLPKYYRRKNKFTL 71
>sp|P36083|YKH5_YEAST Hypothetical 52.1 kDa protein in SMY1-MUD2 intergenic region
Length = 450
Score = 30.4 bits (67), Expect = 5.2
Identities = 23/69 (33%), Positives = 31/69 (44%)
Frame = +1
Query: 52 YFNNVMTVFNKLDGQQASINNDFLTNFVAYINRAYPSKKSEYIIEIWFYCVCHDINNEDL 231
Y N FN L N + V Y+N Y KKS Y IE + C+ H N D+
Sbjct: 49 YGNRRKLTFNFLKHTNMENTNYDVITSVGYLNEKYGLKKSHY-IEKFIKCI-HRKINIDV 106
Query: 232 NSIMDSEVS 258
+ I D+ V+
Sbjct: 107 SKITDAYVN 115
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 79,181,198
Number of Sequences: 369166
Number of extensions: 1557709
Number of successful extensions: 4202
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4037
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4198
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6679696800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)