Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_022_K19
(508 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P06872|TRY2_CANFA Anionic trypsin precursor 81 1e-15
sp|P00762|TRY1_RAT Anionic trypsin I precursor (Pretrypsino... 80 2e-15
sp|P08426|TRY3_RAT Cationic trypsin III precursor (Pretryps... 79 6e-15
sp|Q29463|TRY2_BOVIN Anionic trypsin precursor 78 1e-14
sp|P00763|TRY2_RAT Anionic trypsin II precursor (Pretrypsin... 78 1e-14
sp|P07146|TRY2_MOUSE Anionic trypsin II precursor (Pretryps... 77 2e-14
sp|P35033|TRY3_SALSA Trypsin III precursor 77 3e-14
sp|P32822|TRYB_RAT Trypsin V-B precursor 76 4e-14
sp|P32821|TRYA_RAT Trypsin V-A precursor 76 4e-14
sp|Q03238|GRAM_RAT Granzyme M precursor (Met-ase) (Natural ... 76 4e-14
>sp|P06872|TRY2_CANFA Anionic trypsin precursor
Length = 247
Score = 81.3 bits (199), Expect = 1e-15
Identities = 53/169 (31%), Positives = 86/169 (50%), Gaps = 1/169 (0%)
Frame = +1
Query: 1 DADWKIINGSRTVLHEYPEIVSITQDGEHVCGGTLLNGNTVLTAAHCIWEMRYWTKEWLE 180
D D KI+ G + P VS+ G H CGG+L++ V++AAHC
Sbjct: 19 DDDDKIVGGYTCEENSVPYQVSLNA-GYHFCGGSLISDQWVVSAAHCYKS---------- 67
Query: 181 YHQVKVVIGEYNVNQLEGTEKIYQVTKTIMHPSYKREKAYVDGNDVAVIKFNG-FISNYD 357
+++V +GEYN++ LEG E+ K I HP+Y +++ ND+ +IK + + N
Sbjct: 68 --RIQVRLGEYNIDVLEGNEQFINSAKVIRHPNY---NSWILDNDIMLIKLSSPAVLNAR 122
Query: 358 VEYIKYVKLDTNSISKPGDRCVVVGWGSMLPDGEDTAEYLQKADLNIVS 504
V I + + + PG +C++ GWG+ L G + E LQ D I++
Sbjct: 123 VATISLPR----ACAAPGTQCLISGWGNTLSSGTNYPELLQCLDAPILT 167
>sp|P00762|TRY1_RAT Anionic trypsin I precursor (Pretrypsinogen I)
Length = 246
Score = 80.5 bits (197), Expect = 2e-15
Identities = 51/167 (30%), Positives = 84/167 (50%), Gaps = 1/167 (0%)
Frame = +1
Query: 7 DWKIINGSRTVLHEYPEIVSITQDGEHVCGGTLLNGNTVLTAAHCIWEMRYWTKEWLEYH 186
D KI+ G H P VS+ G H CGG+L+N V++AAHC
Sbjct: 21 DDKIVGGYTCPEHSVPYQVSLNS-GYHFCGGSLINDQWVVSAAHCYKS------------ 67
Query: 187 QVKVVIGEYNVNQLEGTEKIYQVTKTIMHPSYKREKAYVDGNDVAVIKFNGFIS-NYDVE 363
+++V +GE+N+N LEG E+ K I HP+Y ++ ND+ +IK + + N V
Sbjct: 68 RIQVRLGEHNINVLEGDEQFINAAKIIKHPNY---SSWTLNNDIMLIKLSSPVKLNARVA 124
Query: 364 YIKYVKLDTNSISKPGDRCVVVGWGSMLPDGEDTAEYLQKADLNIVS 504
+ ++ + G +C++ GWG+ L +G + + LQ D ++S
Sbjct: 125 PVAL----PSACAPAGTQCLISGWGNTLSNGVNNPDLLQCVDAPVLS 167
>sp|P08426|TRY3_RAT Cationic trypsin III precursor (Pretrypsinogen III)
Length = 247
Score = 79.0 bits (193), Expect = 6e-15
Identities = 54/169 (31%), Positives = 81/169 (47%), Gaps = 1/169 (0%)
Frame = +1
Query: 1 DADWKIINGSRTVLHEYPEIVSITQDGEHVCGGTLLNGNTVLTAAHCIWEMRYWTKEWLE 180
D D KI+ G + P VS+ G H CGG+L+N V++AAHC
Sbjct: 20 DDDDKIVGGYTCQKNSLPYQVSLNA-GYHFCGGSLINSQWVVSAAHCYKS---------- 68
Query: 181 YHQVKVVIGEYNVNQLEGTEKIYQVTKTIMHPSYKREKAYVDGNDVAVIKFNGFIS-NYD 357
+++V +GE+N++ +EG E+ K I HPSY A ND+ +IK N + N
Sbjct: 69 --RIQVRLGEHNIDVVEGGEQFIDAAKIIRHPSY---NANTFDNDIMLIKLNSPATLNSR 123
Query: 358 VEYIKYVKLDTNSISKPGDRCVVVGWGSMLPDGEDTAEYLQKADLNIVS 504
V + + S G +C+V GWG+ L G + LQ D ++S
Sbjct: 124 VSTVSLPR----SCGSSGTKCLVSGWGNTLSSGTNYPSLLQCLDAPVLS 168
>sp|Q29463|TRY2_BOVIN Anionic trypsin precursor
Length = 247
Score = 78.2 bits (191), Expect = 1e-14
Identities = 52/161 (32%), Positives = 82/161 (50%), Gaps = 1/161 (0%)
Frame = +1
Query: 1 DADWKIINGSRTVLHEYPEIVSITQDGEHVCGGTLLNGNTVLTAAHCIWEMRYWTKEWLE 180
D D KI+ G + P VS+ G H CGG+L+N V++AAHC +
Sbjct: 19 DDDDKIVGGYTCAENSVPYQVSLNA-GYHFCGGSLINDQWVVSAAHCY-----------Q 66
Query: 181 YHQVKVVIGEYNVNQLEGTEKIYQVTKTIMHPSYKREKAYVDGNDVAVIKFN-GFISNYD 357
YH ++V +GEYN++ LEG E+ +K I HP Y ++ ND+ +IK + + N
Sbjct: 67 YH-IQVRLGEYNIDVLEGGEQFIDASKIIRHPKY---SSWTLDNDILLIKLSTPAVINAR 122
Query: 358 VEYIKYVKLDTNSISKPGDRCVVVGWGSMLPDGEDTAEYLQ 480
V + L ++ + G C++ GWG+ L G + + LQ
Sbjct: 123 VSTL----LLPSACASAGTECLISGWGNTLSSGVNYPDLLQ 159
>sp|P00763|TRY2_RAT Anionic trypsin II precursor (Pretrypsinogen II)
Length = 246
Score = 78.2 bits (191), Expect = 1e-14
Identities = 51/164 (31%), Positives = 81/164 (49%), Gaps = 1/164 (0%)
Frame = +1
Query: 1 DADWKIINGSRTVLHEYPEIVSITQDGEHVCGGTLLNGNTVLTAAHCIWEMRYWTKEWLE 180
D D KI+ G + P VS+ G H CGG+L+N V++AAHC
Sbjct: 19 DDDDKIVGGYTCQENSVPYQVSLNS-GYHFCGGSLINDQWVVSAAHCYKS---------- 67
Query: 181 YHQVKVVIGEYNVNQLEGTEKIYQVTKTIMHPSYKREKAYVDGNDVAVIKFNGFIS-NYD 357
+++V +GE+N+N LEG E+ K I HP++ R+ ND+ +IK + + N
Sbjct: 68 --RIQVRLGEHNINVLEGNEQFVNAAKIIKHPNFDRKTL---NNDIMLIKLSSPVKLNAR 122
Query: 358 VEYIKYVKLDTNSISKPGDRCVVVGWGSMLPDGEDTAEYLQKAD 489
V + +S + G +C++ GWG+ L G + + LQ D
Sbjct: 123 VATVAL----PSSCAPAGTQCLISGWGNTLSSGVNEPDLLQCLD 162
>sp|P07146|TRY2_MOUSE Anionic trypsin II precursor (Pretrypsinogen II)
Length = 246
Score = 77.4 bits (189), Expect = 2e-14
Identities = 51/168 (30%), Positives = 84/168 (50%), Gaps = 1/168 (0%)
Frame = +1
Query: 1 DADWKIINGSRTVLHEYPEIVSITQDGEHVCGGTLLNGNTVLTAAHCIWEMRYWTKEWLE 180
D D KI+ G P VS+ G H CGG+L+N V++AAHC
Sbjct: 19 DDDDKIVGGYTCRESSVPYQVSLNA-GYHFCGGSLINDQWVVSAAHCY------------ 65
Query: 181 YHQVKVVIGEYNVNQLEGTEKIYQVTKTIMHPSYKREKAYVDGNDVAVIKFNGFIS-NYD 357
++++V +GE+N+N LEG E+ K I HP+Y ++ ND+ +IK ++ N
Sbjct: 66 KYRIQVRLGEHNINVLEGNEQFVDSAKIIRHPNY---NSWTLDNDIMLIKLASPVTLNAR 122
Query: 358 VEYIKYVKLDTNSISKPGDRCVVVGWGSMLPDGEDTAEYLQKADLNIV 501
V + +S + G +C++ GWG+ L +G + + LQ D ++
Sbjct: 123 VASVPL----PSSCAPAGTQCLISGWGNTLSNGVNNPDLLQCVDAPVL 166
>sp|P35033|TRY3_SALSA Trypsin III precursor
Length = 238
Score = 76.6 bits (187), Expect = 3e-14
Identities = 54/169 (31%), Positives = 83/169 (49%)
Frame = +1
Query: 1 DADWKIINGSRTVLHEYPEIVSITQDGEHVCGGTLLNGNTVLTAAHCIWEMRYWTKEWLE 180
D D KI+ G + S+ Q G H CGG+L++ V++AAHC
Sbjct: 11 DEDDKIVGGYECRKNSASYQASL-QSGYHFCGGSLISSTWVVSAAHCYKS---------- 59
Query: 181 YHQVKVVIGEYNVNQLEGTEKIYQVTKTIMHPSYKREKAYVDGNDVAVIKFNGFISNYDV 360
+++V +GE+N+ EGTE+ K IMHPSY ND+ +IK + S
Sbjct: 60 --RIQVRLGEHNIAVNEGTEQFIDSVKVIMHPSYNSRNL---DNDIMLIKLSKPASLN-- 112
Query: 361 EYIKYVKLDTNSISKPGDRCVVVGWGSMLPDGEDTAEYLQKADLNIVST 507
Y+ V L +S + G RC+V GWG++ + + L+ DL I+S+
Sbjct: 113 SYVSTVAL-PSSCASSGTRCLVSGWGNLSGSSSNYPDTLRCLDLPILSS 160
>sp|P32822|TRYB_RAT Trypsin V-B precursor
Length = 246
Score = 76.3 bits (186), Expect = 4e-14
Identities = 57/170 (33%), Positives = 85/170 (50%), Gaps = 2/170 (1%)
Frame = +1
Query: 1 DADWKIINGSRTVLHEYPEIVSITQDGEHVCGGTLLNGNTVLTAAHCIWEMRYWTKEWLE 180
D D +I+ G H P VS+ G H+CGG+L+ VL+AAHC
Sbjct: 20 DNDDRIVGGYTCQEHSVPYQVSLNA-GSHICGGSLITDQWVLSAAHC------------- 65
Query: 181 YH-QVKVVIGEYNVNQLEGTEKIYQVTKTIMHPSYKREKAYVDGNDVAVIKFNGFIS-NY 354
YH Q++V +GE+N+ ++EG E+ K I+HP Y +K VD ND+ +IK + N
Sbjct: 66 YHPQLQVRLGEHNIYEIEGAEQFIDAAKMILHPDY--DKWTVD-NDIMLIKLKSPATLNS 122
Query: 355 DVEYIKYVKLDTNSISKPGDRCVVVGWGSMLPDGEDTAEYLQKADLNIVS 504
V I + + G C+V GWG +L G ++ LQ D ++S
Sbjct: 123 KVSTIPLPQYCPTA----GTECLVSGWG-VLKFGFESPSVLQCLDAPVLS 167
>sp|P32821|TRYA_RAT Trypsin V-A precursor
Length = 246
Score = 76.3 bits (186), Expect = 4e-14
Identities = 57/170 (33%), Positives = 85/170 (50%), Gaps = 2/170 (1%)
Frame = +1
Query: 1 DADWKIINGSRTVLHEYPEIVSITQDGEHVCGGTLLNGNTVLTAAHCIWEMRYWTKEWLE 180
D D +I+ G H P VS+ G H+CGG+L+ VL+AAHC
Sbjct: 20 DNDDRIVGGYTCQEHSVPYQVSLNA-GSHICGGSLITDQWVLSAAHC------------- 65
Query: 181 YH-QVKVVIGEYNVNQLEGTEKIYQVTKTIMHPSYKREKAYVDGNDVAVIKFNGFIS-NY 354
YH Q++V +GE+N+ ++EG E+ K I+HP Y +K VD ND+ +IK + N
Sbjct: 66 YHPQLQVRLGEHNIYEIEGAEQFIDAAKMILHPDY--DKWTVD-NDIMLIKLKSPATLNS 122
Query: 355 DVEYIKYVKLDTNSISKPGDRCVVVGWGSMLPDGEDTAEYLQKADLNIVS 504
V I + + G C+V GWG +L G ++ LQ D ++S
Sbjct: 123 KVSTIPLPQYCPTA----GTECLVSGWG-VLKFGFESPSVLQCLDAPVLS 167
>sp|Q03238|GRAM_RAT Granzyme M precursor (Met-ase) (Natural killer cell granular
protease) (RNK-Met-1)
Length = 258
Score = 76.3 bits (186), Expect = 4e-14
Identities = 51/167 (30%), Positives = 82/167 (49%), Gaps = 2/167 (1%)
Frame = +1
Query: 13 KIINGSRTVLHEYPEIVSITQDGEHVCGGTLLNGNTVLTAAHCIWEMRYWTKEWLEYHQV 192
+II G V H P +VS+ H+CGG L++ VLTAAHC+ E Q+
Sbjct: 20 QIIGGREAVPHSRPYMVSLQNTKSHMCGGVLVHQKWVLTAAHCLSE---------PLQQL 70
Query: 193 KVVIGEYNVNQLEGTEKIYQVTKTIMHPSYKREKAYVDGNDVAVIKFNGFISNYDVEYIK 372
K+V G ++++ + + + + I HP Y + ND+A++K +G + + +K
Sbjct: 71 KLVFGLHSLHDPQDPGLTFYIKQAIKHPGYNLKYE----NDLALLKLDGRVK--PSKNVK 124
Query: 373 YVKLDTNSISKP--GDRCVVVGWGSMLPDGEDTAEYLQKADLNIVST 507
+ L KP G RC GWG G+ A+ LQ+ DL ++ T
Sbjct: 125 PLALPRKPRDKPAEGSRCSTAGWGITHQRGQ-LAKSLQELDLRLLDT 170
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.316 0.135 0.417
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 54,369,436
Number of Sequences: 369166
Number of extensions: 995660
Number of successful extensions: 3706
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3360
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3451
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3206272875
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)